This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ra...This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.展开更多
Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a l...Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a lot relationships between CA125 level and liver cirrhosis,this case had a obvious increased CA125 level in the serum,hydrothorax and ascites,following by the liver cirrhosis in a short time,and then died of upper gastrointestinal bleeding.By now we guess that CA125 level could forecast the liver cirrhosis following by hepatic veno-occlusive,which will become the prognosis of the hepatic veno-occlusive.展开更多
Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(m...Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.展开更多
Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflamm...Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflammatory activity of SA extracts by petroleum ether（PE）, ethyl acetate（EtOAc）, and H2O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF--α, and IL-6 production in RAW 264.7 cells（P0.01）, suggesting that this extract possessed potent anti--inflammatory activity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p--hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the Et -OAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti--inflammatory agent.展开更多
文摘This paper aimed to optimize extracting conditions of SOD in Sedum aizoon by response surface method. First,the single factor optimization tests were carried out. Three single factors were time,p H and liquid-solid ratio respectively. By Design expert 8. 0. 5 software,17 kinds of test scheme were designed. The protein yield of each scheme was obtained,and Design expert 8. 0. 5 software was used to conduct variance analysis and interactive analysis among factors,to obtain the corresponding mathematical model. The results showed that in single factor experiments,the optimal time was 1 h,p H was 7. 8,and liquid-solid ratio was 15:1. The interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon was time of 0. 98 h,p H of 7. 79,liquid-solid ratio of 13. 71:1. By the method of pyrogallol autoxidation,the extracted SOD enzyme activity was measured,namely 12 U/g. The results could provide a reference for further utilization and research of Sedum aizoon resource.
文摘Nowadays hepatic veno-occlusive disease is mainly caused by Sedum aizoon in China,and its prognosis dependents on the dosage and courses of the Sedum aizoon treatment but lacks other objective indicators.There are a lot relationships between CA125 level and liver cirrhosis,this case had a obvious increased CA125 level in the serum,hydrothorax and ascites,following by the liver cirrhosis in a short time,and then died of upper gastrointestinal bleeding.By now we guess that CA125 level could forecast the liver cirrhosis following by hepatic veno-occlusive,which will become the prognosis of the hepatic veno-occlusive.
基金The work was supported by the Natural Science Foundation Guidance Project of Liaoning(2018055455)the Young and Middle-aged Key Talent Training Program of Shenyang Pharmaceutical Universitythe Foundation of Education Department of Liaoning Province(2017LQN09).
文摘Fingerprint analysis of Sedum aizoon L.was carried by High-performance liquid chromatography.There were 27 common peaks in the fingerprint of 10 samples.Eight of these common peaks,peaks 1(arbutin),4(gallic acid),10(myricetin-3-O-β-D-glucopyranoside),14(myricetin-3-O-α-L-rhamnopyranoside),19(quercetin-3-O-α-L-rhamnopyranoside),22(kaempferol-3-O-α-L-rhamnopyranoside),24(quercetin)and 26(kaempferol)were identified by reference substances.Moreover,six flavonoid compounds were simultaneously determined by the above method.By comparing with the constituents in the aerial parts,roots and flowers,it’s easy found that the aerial parts of S.aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots,while the flavonoid glycosides largely existing in the flowers.In addition,an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S.aizoon.This study may provide a preliminary reference for the quality control of S.aizoon.
基金Quality guarantee system of Chinese herbal medicines(Grant No.201507002)Key project of science and technology of Fujian province(Grant No.2014Y0053)youth project of Fujian Provincial Health and Family Planning Commission(Grant No.2015--1--77)
文摘Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflammatory activity of SA extracts by petroleum ether（PE）, ethyl acetate（EtOAc）, and H2O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF--α, and IL-6 production in RAW 264.7 cells（P0.01）, suggesting that this extract possessed potent anti--inflammatory activity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p--hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the Et -OAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti--inflammatory agent.
