Ameliorative effects of standardized extract from Trigonella foenum-graecum L.seeds on painful peripheral neuropathy in rats

Objective:To evaluate the effects of the standardized extract of fenugreek(Trigonella foenumgraecum L.Family:Leguminasae) seed(IND01) in animal models of peripheral neuropathy. Methods:IND01 was prepared from fenugreek seeds and standardized by high performance liquid chromatography to a marker compound,trigonelline.The effects of daily oral administration of IND01(50,100 and 200 mg/kg) were studied in rats after partial sciatic nerve ligation(PSNL) and sciatic nerve crush injury(SNCI) during 30-days period.The measurements on thermal hyperalgesia(TH),motor function test(MFT) score and motor nerve conduction velocity (MNCV) were recorded.Results:IND01 offered sustained protection against TH and deranged MFT scores in both models from 7-day onwards.Fifteen days of daily oral administration of IND01 restored MNCV reduction in rats with SNCI but not with PSNL.Conclusions:IND01 was found to be effective in rat models of painful peripheral neuropathy.

Ameliorative properties of Iranian Trigonella foenum-graecum L. seeds and Punica granatum L. peel extracts in streptozotocin-induced experimental diabetic guinea pigs

Objective: To assess the ameliorative properties of Iranian Trigonella foenum-graecum L.(T. foenum-graecum) seeds and Punica granatum L.(P. granatum) peel extracts against streptozotocin-induced diabetes in male guinea pigs.Methods: Sixty guinea pigs were divided into six groups(10 guinea pigs per group).Group 1 consisted of normal animals. Groups 2 and 3 were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract alone, respectively. Group 4 was treated with streptozotocin only; whereas Groups 5 and 6 receiving streptozotocin were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract, respectively.All animals were treated for 30 days, and the body weight, blood and liver biochemical parameters were measured.Results: Guinea pigs exposed to streptozotocin showed an alteration in body weight gain, fasting glucose level, kidney function parameters(blood urea nitrogen and creatinine) as well as decreased serum and hepatic total protein level. In addition, it increased the cholesterol and triglyceride level, while decreasing the hepatic glucose-6 phosphate dehydrogenase activity, glycogen, glutathione content and hepatic catalase activity. Oral treatment with T. foenum-graecum seeds and P. granatum peel extracts revealed significant protective properties with respect to body weight gain and other biochemical parameters studied.Conclusions: The Iranian T. foenum-graecum seeds and P. granatum peel extracts are significantly potent in ameliorating diabetic condition induced by streptozotocin and improving various biochemical parameters in serum and liver of guinea pigs.

Aqueous and organic extracts of Trigonella foenum-graecum L.inhibit the mycelia growth of fungi

Aqueous extracts from various plant parts of fenugreek(3%)(aerial parts:leaves and stems(LS),roots(R),ground seeds(GS)and not ground seeds(NGS))and petroleum ether,ethyl acetate and methanolic fractions of the aerial parts were assayed to determine their antifungal potential against Botrytis cinerea,Fusarium graminearum,Alternaria sp.,Pythium aphanidermatum,and Rhizoctinia solani.All fenugreek plant parts showed antifungal potential and the magnitude of their inhibitory effects was species and plant parts d...

胡芦巴种子化学成分的研究

对胡芦巴 (Trigonellafoenum graecumL .)种子的物理组成及主要化学组成进行了研究 .实验结果表明 ,胡芦巴种子物理组成中 ,种皮占 15 5 2 %,内胚乳占 31 14 %,胚芽、子叶占 5 3 34%;种子千粒重为 14 78%,容重为2 0 0 7%.测定了胡芦巴种子 18种氨基酸和 8种微量金属元素的含量 .粗蛋白含量为 5 4 0 6 %,种子中聚糖含量为8 72 %.

压力对胡卢巴籽超临界萃取的影响

为开发新的烟用香料,对天然香料植物胡卢巴籽进行了超临界流体萃取(SFE)试验,利用GC/MS对萃取物进行了分析,并对其在卷烟中的作用进行了评价。结果表明,在萃取温度50℃和萃取压力25MPa条件下得到的萃取物加香效果最好,且其加香加料效果均好于普通溶剂萃取得到的胡卢巴酊。在该萃取物中鉴定出52种化合物。

胡芦巴中皂苷成分的研究Ⅲ.新皂苷D的分离纯化及化学结构测定

目的对胡芦巴Trigonellafoenum-graecum种子所含的皂苷成分做深入研究。方法采用硅胶H柱色谱、干柱色谱等从总皂苷中分离纯化单一皂苷成分,并应用13C-NMR、FAB-MS、DEPT等光谱及部分水解获得的次生苷的方法测定其化学结构。结果从总皂苷中分离出的一个新皂苷D为六分子糖与薯蓣皂苷元构成的六糖苷,经测定皂苷D的结构为:薯蓣皂苷元-3-O-α-L-鼠李吡喃糖(1→3)-β-D-葡萄吡喃糖(1→4)-α-L-鼠李吡喃糖[(1→3)-α-L-鼠李吡喃糖](1→4)-β-D-葡萄吡喃糖(1→4)-β-D-葡萄吡喃糖苷。结论皂苷D是一个新的六糖皂苷。

栽培密度对胡芦巴生产性能和生物量分配的影响

应用盆栽试验研究了栽培密度对胡芦巴（Trigonella foenum-graecumL.）形态特征和生物量分配的影响.结果表明,胡芦巴株高介于16～23 cm之间,分枝数2～3个,节数为11节左右,6～8节的节间距呈中间长两头短的趋势,复叶长较复叶宽短,顶生小叶长与宽除3株/盆处理外,其他处理下均成长∶宽为3∶2的特征;根冠比为0.55 ～0.61,茎叶比为0.64 ～0.73,干鲜比为0.13 ～0.19,光合产物茎叶根的于重分配比例为13∶ 18∶1至32∶42∶1,生物量测算结果表明,胡芦巴单位面积鲜产为8 306.2～8 324.8 kg/hm2,产干草505.9～512.7kg/hm2,侧根较发达,占总根系的50％ ～ 62％.探明胡芦巴是一种侧根较发达,叶量丰富,适口性较强的牧草,适宜于水土保持和护坡的种植.建议栽培密度566～890株/m2,可适当密植.

胡芦巴对急性化学性肝损伤的保护作用

目的 探讨胡芦巴对急性化学性肝损伤的保护作用及其机理。方法 采用四氯化碳和D 氨基半乳糖所致小鼠急性肝损伤的模型 ,染毒前不同时间、不同剂量给药对小鼠肝功能指标ALT和AST的影响 ,同时观察脂质过氧化水平。结果 胡芦巴有效地抑制CCl4 和D 氨基半乳糖所致小鼠急性肝损伤的血清ALT和AST的升高 ,并呈现良好的剂量 效应关系 ;不同治疗时间、每日给药次数对疗效有很大影响 ,每日 3次给药 ,治疗 3天以上效果较好 ;给药后 ,肝脏MDA水平降低 ,GSH Px升高 ,呈现剂量 反应关系 ,以上各指标与对照组 (不给药组 )均有显著性差异。结论 胡芦巴提取物能有效地保护小鼠急性化学性肝损伤 ,其机理可能是抑制脂质过氧化 ,增加GSH

不同地区胡卢巴种子和苗期特性的初步研究

以宁夏本地、国内其他省份及国外引进的38份胡卢巴（Trigonella foenum-graecumL.）种质资源（以下简称种源）为材料，调查种子形态特征、田间苗期基本形态特征及各阶段的生育时期.结果表明，不同种源胡卢巴种皮颜色主要为黄绿色或金黄色，种子形状为斜方形或卵圆形，种子最小的是58和59种源.各胡卢巴种源全生育期集中在94-95 d，但各阶段所持续的天数有所不同.各种源胡卢巴在苗高、子叶叶缘色、复叶叶色、小叶叶基部色、叶柄色、主茎色、主茎有无毛等性状上均表现不同特点.种源58，59植株细弱，呈匍匐状，小叶为阔卵圆形或倒心形，叶片薄而软；其他种源植株坚挺，直立型，小叶均为长卵圆形，质地较厚.

胡芦巴种子不同溶剂提取物及提取工艺对植物果实致病菌的抑菌作用初探

【目的】探究胡芦巴种子提取物对引起植物果实病变菌的抑菌作用,并筛选最佳溶剂的提取工艺。【方法】以乙醇、乙酸乙酯和水作为不同溶剂对胡芦巴种子进行提取,对引起植物果实病变的苹果炭疽菌、苹果腐烂菌及冬枣腐烂菌进行抑菌实验,通过正交试验筛选最佳溶剂的提取工艺。【结果】胡芦巴种子不同溶剂提取物均对苹果炭疽菌和苹果腐烂菌群具有抑菌作用,其中乙醇提取物抑菌效果高于乙酸乙酯和蒸馏水提取物,胡芦巴种子抑菌作用的最佳提取工艺为,以80%的乙醇提取,固液比为1:15,在60℃下提取2 h的抑菌效果最佳。【结论】胡芦巴种子提取物具有抑菌作用,且提取因素影响抑菌效果的顺序为乙醇浓度>提取时间>提取温度>液固比。