Mapping of three QTLs for seed setting and analysis on the candidate gene for qSS-1 in rice(Oryza sativa L.)

The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility （seed setting） is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 （indica） and Nipponbare （japonica） and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility （seed setting） and understanding the genetic bases of pollen sterility.

Changes of Seed Germination during the Year

It is possible to conclude that typical course of graph which analyse course of germinability during the year at every cultivar is observable. Germination usually shows a minimum in December, but mainly in January and in February. Maximum of germinability is observed in the spring and summer time. This phenomenon isn’t result of the dormancy or secondary dormancy because during of long term seed storage, germinability has similar curve every year, only number of germinative seeds stepwise decrease every year at every month of storage. Every species or cultivar of crops has a typical course of the germinability during the year. There are also species (crops), that have germination process during the year almost constant i.e., without variation during the year, but it is a minority of genotypes, rather exceptions. The annual course of germination is nearly identical to the annual course of the magnetic declination. From the theoretical practical view, there is possibility (but not certainty!) that in case of testing the seed germination at begin or end of year at genotypes with large variability of germinability during the year, that this phenomenon have negative impact on seed certification by companies producing seeds for cultivation practice in some species.

Factors Influencing the Outcrossed Seed-Setting Obstacles of Photo-thermo Sensitive Genie Male Sterile Rice

Using photo-thermo sensitive genie male rice (PTGMS) Pei' ai 64S, W7415S, W6154S, N26S, Annong S, Nongken 58S, 7001S and 5088S as female parents and conventional indica lines 8258 and U89 as male parents, the factors affecting outcrossed seed-setting were analyzed. The PTGMS had obstacles in outcrossed seed setting influenced by inheritance and environment at varying degrees. Environmental temperature was regarded as the main factor that resulted in the outcrossed seed-setting obstacles. The sensitive stage was at the early stage of grain filling for outcrossed seed setting. There existed remarkable differences at the sensitivity stage, the duration of sensitive period, the sensitive level and the effective level of outcrossed seed-setting obstacles caused by environmental temperature among different PTGMS lines. Therefore, attention should be paid to outcrossed seed-setting obstacles in selection and utilization of PTGMS lines.

Effect of Extreme Natural High Temperature on Seed Setting Rate of Rice(Oryza sativa L.) at Heading and Flowering Stage

The seed setting rates of total 198 rice cultivars （lines） at heading and flowering stage were investigated under the condition of extreme natural high tem- perature in 2013 so as to analyze the effect of extreme natural high temperature on seed setting rate of different rice cultivar （line）. The results showed that the contin- uous high temperature showed certain effects on the seed setting rates of tested materials, and significant differences were shown in seed setting rate among differ- ent rice cultivars （lines）. The seed setting rates differed significantly among indica F1 hybrids derived from different sterile and restorer lines, indicating that the sterile and restorer lines had great effects on heat tolerances of different F~ hybrids. The cor- relation analysis showed that the seed setting rates of conventional indica restorer lines and conventional japonica rice cultivars （lines） were negatively related to the daily highest temperature （P〉0.05）, and the seed setting rates of indica F1 hybrids were positively related to the seed setting rates of their restorer lines. Total four in- dica restorer lines, including Ninghuiguangkangzhan, Shuhui 527, Chenghui 3203 and Xianyin-8, and four new japonica rice cultivars （lines）, including Wuyinjinghui （B2）, Nanjing 4//W3660/Nanjing 44 （B12） and Wuyun 2330/JD6011 （B22） were pre- liminarily screened, and their seed setting rates were all close to the normal level （90%）. The screened rice cultivars （lines） showed higher heat tolerances.

Effect of Enhanced UV-B Radiation on Seed Setting Rate and 1 000-grain Weight of Indica Hybrid Rice Restorer Lines

[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps （control） were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation.

Studies on Seed Setting Stability in Associated with Temperature for a Two-Line Hybrid Rice,Liangyoupeijiu

When being planted in wide areas in southern China (23°23' - 33°23' N, 98°35' - 120°19' E, sea level above 2.7 - 1285 m), Liangyoupeijiu (LYPJ), a two-line hybrid rice combination, showed a seed setting rate of 75.2 - 77.2%, which was lower by 4.3 - 7.5 percent point than that of an indica hybrid rice Shanyou 63 (CK), with similar values of grain yield and coefficients of variation to CK. Sowing during 5-25th of May in Nanjing (32°3' N, 118°48' E), China, LYPJ headed before 4th September, and gave a seed setting rate of 75 - 90%, and grain yield over 1 kg/m2. If the sowing date was delayed to 14 - 15th, June, its heading date would be as late as 17th - 21st September, and seed setting rate would be declined by 10 - 15% in comparison with that on a suitable sowing date. When flowering took place at an average daily temperature range of 13.7 - 28.5℃ for five days, the spikelet fertility (SF) would be increased by 1.9- 10.7%, for each increment of 1℃. The suitable (SF≥90%) and safe (SF≥75%) temperatures for flowering stage were indicated to be 26.5℃ and 22.9℃, respectively. To get a high and stable seed set in cultivating LYPJ, it was recommended that LYPJ would be planted in the areas south of 34癗, and the favorable average daily temperature during flowering stage should be at 26 - 28℃.

Effect of ^(211)At treating pollen and stigma on generative cells and seed setting of rice

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Distribution of Rice Grains on Panicle Axis and Its Relationship with Seed-Setting Ability in Liaoning, China

Studies were conducted with rice varieties developed in recent years in Liaoning Province of China as test materials. Results showed that the varieties tested could be divided into three types： （1） the high-yielding （HYV）, （2） the mediumyielding （MYV）, and （3） the low-yielding （LYV）. The panicle population was in the order of HYV〈 MYV 〈 LYV, while the grain number per panicle and seed-setting rate were just opposite. There was no significant difference in the 1 000-grain weight. Biomass was higher in HYV than in MYV and LYV, while the harvest index was higher in HYV and MYV than in LYV. Yield was inversely correlated with panicle population, but positively correlated with the grain number per panicle, seed-setting rate, the number of primary and secondary branch grains, seed density and harvest index. Yield was most closely linked with the grain number per panicle which was determined chiefly by the number of secondary branch grains and to a lesser degree by seed density and the number of primary branch grains. The seed-setting rate of the secondary branches was positively correlated with that of the whole panicle and yield. The peak value of primary branches for HYV, MYV and LYV was 12-14, 10-13 and 8-12, respectively. LYV had fewer secondary branch grains, while MYV and HYV had more. Such grains distributed mainly in the middle and lower parts of panicle axis for LYV and in the middle and upper parts for MYV and HYV.

Treatments of Pollen with Different Solvents Affect the Seed Setting Rate of Rice(Oryza sativa L.)

This study aimed to investigate the effects of treatments of rice pollen with different solvents on the seed setting rate.Rice pollen was treated with ultrapure water,0.5%dimethyl sulfoxide,ethanol(1%,5%,and 8%),and 0.5%dimethyl sulfoxide+8%ethanol,and the pollen without treatment was taken as the control.Then,crossing was conducted with the treated rice plants as the male parents and plants subjected to emasculation by warm water as the female parents.The seed setting rates of the male and female parents were then recorded.The results showed that the treatments with different solvents significantly decreased the seed setting rates of both male and female parents.The treatment with 1%ethanol resulted in the highest seed setting rates for both male and female parents,while the treatment with 0.5%dimethyl sulfoxide resulted in the lowest seed setting rates for both parents.Although these solvents affected the seed setting rate of rice,they served as intermediate solvents to dissolve chemicals used in the induction of rice haploids.This study layed a technical foundation for subsequent chemical induction of rice haploids.

Floral traits and pollination system of Zygophyllum xanthoxylum in the managed and wild populations in an arid region of Northwest China

Zygophyllum xanthoxylum, which belongs to Sarcozygium of Zygophyllaceae, is one of the ecologically important species in Northwest China. In order to understand the pollination system of Z. xanthoxylum, we investigated the following characteristics of this species in the Urat Desert-grassland Research Station in western Inner Mongolia of China： flowering dynamics, pollen viability, pollen limitation, floral visitors and breeding system. The results showed that the flowering period and flowering peak were different between the wild and managed popula- tions, being longer in the managed population. Z. xanthoxylum was pollen-limited, and pollen limitation was more intense in the wild population than in the managed population. Chalicodoma deserticola （Hymenoptera） was found to be the most frequent pollinator in the wild population, while Anthophora fulvitarsis （Hymenoptera） was the most frequent and effective visitor in the managed population. Out-crossing was dominant in the breeding system and self-pollination just played an assistant role to assure the reproduction of Z. xanthoxylum.

Distribution of the Classification Traits in the F_2 Progeny of Two Crosses of indica/japonica in Rice (Oryza sativa)

Comparison of seed setting rate, plant height and Cheng's morphological traits were made between the F_2 progeny of indica-japonica hybrids, 02428/Pei'ai 64 and Taichung 65/Teqing. There existed resemblance normal distribution in plant height, and the distribution had no relationship with the seed setting rate and plant height in F_1 progeny, suggesting that there were no gametic selection for the plant height in the F_2 progeny of indica-japoniea hybrids. An average seed setting rate of the F_2 was equal to the F_1 populations in the recombination of 02428/Pei'ai 64 in which there was an excellent cross compatibility, but the remarkable increase in the seed setting rate from the F_1 to F_2 in Taichung 65/Teqing, indicating that gametic selection only took place in the indica-japonica crosses with low cross compatibility while null or little in the hybrids with high compatibility. There were many intermediate types among the leaf pubescence, color of hull at heading, and length of the first and second panicle internode, and these Cheng's morphological traits was distributed normally; there were also a little intermediate types in hull hairness and phenol reaction with bi-peak distribution; while length to width ratio of grain was inclined to the japonica types obviously showing a skewed distribution in the F2 population. Although Cheng's morphological traits in F_2 populations had inconsistent distribution types, Cheng's morphological traits index showed normal distribution, which suggested that intermediate types, i.e. indicalinous or japonicalinous, were primary in F_2 population.

Studies on the Growth Habits and Characteristics of Two Polyploid Indica-Japonica Hybrid Rice with Powerful Heterosis

Based on a series of polyploid indica-japonica hybrid plant lines obtained from a new breeding strategy of using double predominance of wide cross and polyploidization to breed super flee, two polyploid indica-japonica hybrids, PSR073 and PSR120 were studied in their growth periods to show the powerful heterosis in a larger scale and to study the characteristics of polyploid indica-japonica hybrids more elaborately. The leaf age, tiller growth, flowering habits, and agronomic traits of them were observed to analyze their growth habits and characteristics. The results showed that the.agronomic traits of PSR073 and PSR120, such as the plant height, panicle length, grain length, grain width, and 1000-grain weight, all acquired obvious predominance of polyploidy, and that the seed setting rate was more than 83%. No significant difference was observed between the two tetraploids and common diploids in the leaf age, tiller growth, and flowering habits. It was concluded that the characteristics of the two powerful heterosis polyploid hybrids were different from those of the polyploid rice reported earlier. Wide cross and polyploidization had no negative effects on their growth habits and characteristics; on the contrary these had powerful heterosis. This had provided theoretic and practical evidences for their application to agricultural production.

Seasonal change mediates the shift between resource and pollen limitation in Hedysarum scoparium(Fabaceae)

Theory suggests that with sufficient environmental variation, pollen limitation might be observed at some places or times, and resource limitation at others, but there are no empirical data about the effect of seasonal change on the variation of pollen limitation and resource limitation within a flowering season. In this study, we examined pollen and resource limi- tation by comparing fruit set and seed production in natural- and hand-pollinated Hedysarum scoparium flowers in the middle reaches of the Hexi Corridor region, China, in 2010. We also described a role for the first substantial autumn rainfall in mediating a shift between pollen and resource limitation in H. scoparium, but did not analyze this experimentally Our results indicated that H. scoparium was resource limited at peak flowering during the summer, and was pollen limited at peak flowering during the autumn. The seasonal change （summer to autumn） mediated the shift between pollen and resource limitations in H. scoparium. The shift timing depended on the date of the first autumn rainfall in 2010. Changes in the first substantial rainfall in autumn may affect fruiting of H. scoparium, thus affecting population persistence of this species and development/structure of the local ecosystem if such conditions persist.

Cultivar and Soil Fertilizer Treatment Affect Seed Production of Sweet Yellow Yam (<i>Dioscorea dumetorum</i>) on Highly Acidic Soils of the Western Highlands Region of Cameroon

The popularization of Dioscorea dumetorum (Dioscoreaceae), a nutritious yam species is still marginal due to constraints such as the low interest of research, poor cultural practices, insufficient and expensive seeds, pests, and diseases. The latter pushes producers to use about 50% of their harvest as seed for the next planting season. The lack of a specific fertilizer formulation for yam production on the oxysols of Cameroon is another constraint. This study was aimed at enhancing the availability of quality seeds through the characterization of five yam accessions. One experiment was performed to determine the effect of cultivar and origin of tuber sets on their germination rate. The second concerned the evaluation of cultivars and soil treatment effects on agronomic parameters of yam seedlings. Yam cultivars were subjected to nine fertilizer formulations as follows: T0: no fertilizer;T1: mycorrhizal fungi (MF) + 150 g manure + peanut waste;T2: 25 g chemical fertilizer (20.10.10);T3: MF + peanut waste;T4: 16 g chemical fertilizer (12.6.20);T5: 25 g (20.10.10) +100 g CaO + 150 g manure;T6: 100 g CaO + 150 g manure;T7: 20 g tropicote + 2 g DAP (Diammonium phosphate, a reference starter fertilizer coded N18P46) + 100 g CaO + 150 g manure;and T8: mycorrhiza. These treatments were tested in a factorial design. Results show that Ibo sweet 3 and Mabondji sweet white 1 yam accessions were less affected by anthracnose disease on the aerial parts. Guzang 1 showed the best germination rate and yield, making it appropriate for cultivation in the region. Soil treatments, T2, T5, T7 and T8 significantly increased the yield of yam cultivars. Mycorrhiza treatment alone gave a better response to seed weight, indicating the interest of this biofertilizer as a solution for good seedling production. These fertilizer treatments can be recommended to farmers for more seed production with optimum seed weight (300 g).

Molecular mechanisms controlling seed set in cereal crop species under stress and non-stress conditions

Maximizing seed yield is the ultimate breeding goal in important cereal crop species. Seed set is a key developmental stage in the process of seed formation, which determines grain number, seed mass, and realized yield potential, and can be severely affected by abiotic and biotic stresses. However, seed set can also be substantially reduced by genetic factors even under optimal fertilization conditions. The underlying molecular genetic mechanisms are still obscure. In this review, we elucidate the process of seed set of cereal crop species in detail, including development of floral structures, formation of viable gametes, double fertilization, seed development, and abortion. We discuss how genetic and non-genetic factors affect seed set in different development stages. Finally, we will propose novel strategies to study genetic mechanisms controlling seed set and exploit genetic resources to improve seed set in cereal crop species.

基于Seeds集和成对约束的半监督三支聚类集成

聚类集成使用合适的策略融合多个具有差异性的基聚类成员,能够有效提高聚类结果的稳定性、鲁棒性和准确率。当前聚类集成的研究较少利用已知的先验信息,面对复杂数据时难以刻画对象与类簇之间明确的归属关系。因此,提出一种基于Seeds集和成对约束的半监督三支聚类集成方法。首先,基于已有的标签信息提出一种新的三支标签传播算法构造基聚类成员;其次,提出一种半监督三支聚类集成框架集成基聚类成员,构造出一致性相似矩阵,并利用成对约束信息对该矩阵进行优化调整;最后,将三支谱聚类作为一致性函数对相似矩阵进行聚类,得到最终集成结果。在多个UCI真实数据集上的实验结果表明,与基于类簇的相似分区算法(CSPA)、超图分区算法(HGPA)、元类簇算法(MCLA)、标签传播算法(LPA)、Cop-Kmeans等半监督聚类集成算法相比,所提方法的归一化互信息(NMI)、调整兰德系数(ARI)和F测度在绝大多数据集上取得了最优值,获得了相对更好的聚类集成结果。

播量和行距对箭筈豌豆农艺结实性状及产量的影响

为了探讨豫南本地箭筈豌豆条播种植适宜播量和行距,采用双因素试验设计,分别设3个播量水平和4个行距水平,研究不同播量和行距配置对箭筈豌豆生长发育、鲜草、秸秆及种子产量结实的影响,为完善箭筈豌豆实际生产中的高产栽培技术提供科学理论指导。结果显示,播量一定时,株高、单株鲜干重随行距的增加呈现先增加后减少趋势,且在行距30、40 cm时获得最大值;茎粗和分枝数随行距的增加呈逐渐减少趋势,均在行距20 cm时获得最大值。行距一定时,株高、SPAD值随播量的增加呈逐渐增加趋势;茎粗、分枝数随播量的增加呈逐渐减少趋势。筛选出结实性播量行距最佳配置为:播量18.75 kg/hm^(2)、行距30 cm,播量26.25 kg/hm^(2)、行距30 cm和播量33.75 kg/hm^(2)、行距40 cm。不同播量和行距处理下箭筈豌豆种子、鲜草和秸秆产量差异显著(P<0.05)。种子产量以播量26.25 kg/hm^(2)、行距30 cm最高,达3753 kg/hm^(2);播量33.75 kg/hm^(2)、行距40 cm和播量33.75 kg/hm^(2)、行距30 cm次之,产量分别达3468和3436 kg/hm^(2)。鲜草和秸秆产量均以播量33.75 kg/hm^(2)、行距20 cm最高,达35910和16878 kg/hm^(2);播量33.75 kg/hm^(2)、行距30 cm和播量33.75 kg/hm^(2)、行距40 cm次之,产量分别达26761和13052、23662和12127 kg/hm^(2)。综上,以收获鲜草和秸秆产量为目的,优选播量33.75 kg/hm^(2)和行距20 cm组合配置;收获种子产量优选播量26.25 kg/hm^(2)和行距30 cm组合配置。

不同采集年份大花红景天种子萌发对温度的响应

为确定温度变化对大花红景天(Rhodiola crenulata)种子萌发的影响,本研究以云南省香格里拉市大雪山居群的种子为材料,探究不同采集年份(2014、2015、2016、2019、2020、2021年)种子的萌发对温度(恒温:1、5、10、15、20、25、30、35℃;变温:15℃/5℃、25℃/15℃)的响应机制。结果表明:大花红景天种子萌发温度阈值较宽,1~35℃均可萌发,具有较宽的萌发温度生态位幅度(0.831~0.891),15~25℃萌发效果最佳;变温与相应恒温相比不能显著增加最终萌发率。通过积温模型模拟,种子萌发最低温度(Tb)为−0.49~0.82℃,萌发最适温度(To)为19.38~20.88℃,萌发最高温度(Tc)为34.21~38.76℃,亚适温条件下的种子萌发积温值为40.7~67.11℃·d。综上,大花红景天种子具有“机会主义”萌发行为,萌发最低温度较低且具有较宽的萌发温度窗口,使其可以在早春大量萌发,以保证幼苗拥有较长的生长季。研究结果为保护与利用野生大花红景天资源提供了一些参考。

截干强度对红松结实的影响

为探讨截干强度对红松人工林结实的影响,2017年在通化县三棚林场19年生红松人工林内设置了4个截干强度(截干3轮枝、截干4轮枝、截干5轮枝,CK),2022年和2023年分别对所有处理下结实情况进行调查和分析。结果表明:截干后,红松结实株率、单株平均球果数量和产量与对照相比,均有显著增加(P<0.05),红松单个球果平均质量无明显变化。截干3轮枝时,红松结实株率最高,比CK提高了145.56%;截干5轮枝时,单株平均球果数量和产量最高,比CK分别提高了300.34%和468.04%。

中国沙棘(Hippophae rhamnoides L．ssp．sinensis Rousi)的开花特性及风媒传粉距离的检测

连续两年对雌雄异株的中国沙棘（Hippophae rhamnoides L．ssp．sinensis Rousi）的花期物候、开花式样进行了观察，检测了柱头可授性、花粉活力和花粉一胚珠比，并进行厂人工授粉、套袋实验，检测结实率。结果显示中国沙棘风媒传粉发生在4月下旬或5月上旬，单花花期约7d；传粉盛期在第3天到第5大，柱头的可授性从第2天到第4天或第5天。中国沙棘花先叶开放、花小、无花冠、花药成熟时萼片从两个侧向的纵缝中裂开，形成有利于化粉散布的对流风洞。花粉活力和花粉．胚珠比都很高，具有典型的适应风媒传粉的花部特征和性状。中国沙棘花粉的传播距离受外界环境影响较大，通过重力玻片法检测可以看出，在风速小于3m／s时，主要集中在15-25m之间，当风速大于3m／s时，顺风向可超过85m以外。传粉效率对结实率的影响较大。