To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were ...To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi.展开更多
为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的...为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。展开更多
基金Supported by the Program for the Scientific Research and Technology Development in Guangxi(Guikehe 415104001-22)the Science and Technology Planning Project of Guangxi(Guikegong 1598006-5-13)+2 种基金the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011)the Fund for Science and Technology Development and Scientific Research of Guangxi Academy of Agricultural Sciences(Guinongke 2015JM23,2017JM01,2017JM03)the Innovation-Driven Development Special Fund Project of Guangxi(Guike AA17202019-1&Guike AA17202019-4)
文摘To provide reference for selenium resource exploitation of soil,many selenium-tolerant strains were isolated from selenium-rich soil in Guangxi. The dilution spread plate method and selenium-added culture method were used to screen the selenium-tolerant strains from the soils which were sampled from the main Selenium-rich areas such as Yongfu,Bama,Yulin Hanshan,Guiping and Tengxian. The results showed that 8 strains with high selenium tolerance were obtained,which could tolerate the selenium concentration above 10 000 μg/m L in solid medium. Among the 8 strains,YLB1-33 showed the highest selenium tolerance,and it could still grow weakly in the solid medium with selenium concentration of 29 000 μg/m L. The sequencing of 16 S rD NA and phylogenetic analysis showed that YLB1-6 was identified as Bacillus cereus,BMB2-1 and TXB1-8 were identified as Bacillus pumilus,GPB2-5 was identified as Bacillus thuringiensis,YLB1-26 and YLB1-33 were identified as Bacillus licheniformis,and YLB1-2 and YFB1-8 were identified as Serratia marcescens. The finding of selenium-tolerance strains had potential application value on promoting the utilization of selenium soil resources and the development of selenium-rich agricultural products in Guangxi.
文摘为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。
