The T2/RNase gene family is widespread in eukaryotes,and particular members of this family play critical roles in the gametophytic self-incompatibility(GSI) system in plants.Wild diploid strawberry(Fragaria)species ha...The T2/RNase gene family is widespread in eukaryotes,and particular members of this family play critical roles in the gametophytic self-incompatibility(GSI) system in plants.Wild diploid strawberry(Fragaria)species have diversified their sexual systems via self-incompatible and self-compatible traits,yet how these traits evolved in Fragaria remains elusive.By integrating the published and de novo assembled genomes and the newly generated RNA-seq data,members of the RNase T2 gene family were systematically identified in six Fragaria species,including three self-incompatible species(Fragaria nipponica,Fragaria nubicola,and Fragaria viridis) and three self-compatible species(Fragaria nilgerrensis,Fragaria vesca,and Fragaria iinumae).In total,115 RNase T2 genes were identified in the six Fragaria genomes and can be classified into three classes(Ⅰ-Ⅲ) according to phylogenetic analysis.The identified RNase T2 genes could be divided into 22 homologous gene sets according to amino acid sequence similarity and phylogenetic and syntenic relationships.We found that extensive gene loss and pseudogenization coupled with small-scale duplications mainly accounted for variations in the RNase T2 gene numbers in Fragaria.Multiple copies of homologous genes were mainly generated from tandem and segmental duplication events.Furthermore,we newly identified five S-RNase genes in three self-incompatible Fragaria genomes,including two in F.nipponica,two in F.viridis,and one in F.nubicola,which fit for typical features of a pistil determinant,including highly pistil-specific expression,highly polymorphic proteins and alkaline isoelectric point(pI),while no S-RNase genes were found in all three selfcompatible Fragaria species.Surprisingly,these T2/S-RNase genes contain at least one large intron(>10 kb).This study revealed that the rapid evolution of T2/S-RNase genes within the Fragaria genus could be associated with its sexual mode,and repeated evolution of the self-compatible traits in Fragaria was convergent via losses of S-RNase.展开更多
The region coverage control problem of multiple stratospheric airships system is firstly addressed in this paper.Towards it,we propose a two-layer control framework with the artificial potential field(APF)-based regio...The region coverage control problem of multiple stratospheric airships system is firstly addressed in this paper.Towards it,we propose a two-layer control framework with the artificial potential field(APF)-based region coverage control law and the adaptive tracking control law.The APF-based region coverage control law ensures the coverage task is achieved until every single stratospheric airship ends up performing station keeping where near the respective global minimum point,in which an innovative solution to the local minimum problem is put forward.The adaptive tracking control law is designed to realize motion control using tracking the desired velocity and angular velocity given by coverage control law,with the consideration of several practical control problems as unknown individual differences and external disturbances.To save resources,the combined self-/event-triggered mechanism designed therein significantly reduces the times of state information transmission and control law calculation.The effectiveness of the proposed control framework is verified through simulations.展开更多
William Wordsworth is best known for his poem,The Daffodils.This paper traces the origin of daffodils in Greek mythology,the four important facts in Wordsworth’s life and the poetic theories implied in his poem The D...William Wordsworth is best known for his poem,The Daffodils.This paper traces the origin of daffodils in Greek mythology,the four important facts in Wordsworth’s life and the poetic theories implied in his poem The Daffodils.The most important part shall be the appreciation and analysis of the poem.During this course,readers may find it difficult to understand Wordsworth’s poetic theories of Romanticism.However this is made easier by asking and answering questions and by comparing Chinese and English Romantic poems.展开更多
基金financially supported by the National Key Research and Development Program of China (2018YFD1000107)the open research project of the "Cross-Cooperative Team" of the Germplasm Bank of Wild Species to A.Z.
文摘The T2/RNase gene family is widespread in eukaryotes,and particular members of this family play critical roles in the gametophytic self-incompatibility(GSI) system in plants.Wild diploid strawberry(Fragaria)species have diversified their sexual systems via self-incompatible and self-compatible traits,yet how these traits evolved in Fragaria remains elusive.By integrating the published and de novo assembled genomes and the newly generated RNA-seq data,members of the RNase T2 gene family were systematically identified in six Fragaria species,including three self-incompatible species(Fragaria nipponica,Fragaria nubicola,and Fragaria viridis) and three self-compatible species(Fragaria nilgerrensis,Fragaria vesca,and Fragaria iinumae).In total,115 RNase T2 genes were identified in the six Fragaria genomes and can be classified into three classes(Ⅰ-Ⅲ) according to phylogenetic analysis.The identified RNase T2 genes could be divided into 22 homologous gene sets according to amino acid sequence similarity and phylogenetic and syntenic relationships.We found that extensive gene loss and pseudogenization coupled with small-scale duplications mainly accounted for variations in the RNase T2 gene numbers in Fragaria.Multiple copies of homologous genes were mainly generated from tandem and segmental duplication events.Furthermore,we newly identified five S-RNase genes in three self-incompatible Fragaria genomes,including two in F.nipponica,two in F.viridis,and one in F.nubicola,which fit for typical features of a pistil determinant,including highly pistil-specific expression,highly polymorphic proteins and alkaline isoelectric point(pI),while no S-RNase genes were found in all three selfcompatible Fragaria species.Surprisingly,these T2/S-RNase genes contain at least one large intron(>10 kb).This study revealed that the rapid evolution of T2/S-RNase genes within the Fragaria genus could be associated with its sexual mode,and repeated evolution of the self-compatible traits in Fragaria was convergent via losses of S-RNase.
基金supported by the Postdoctoral Science Foundation of China(Grant No.2020TQ0028)the National Natural Science Foundation of China(No.62173016)Beijing Natural Science Foundation,PRChina(No.4202038)。
文摘The region coverage control problem of multiple stratospheric airships system is firstly addressed in this paper.Towards it,we propose a two-layer control framework with the artificial potential field(APF)-based region coverage control law and the adaptive tracking control law.The APF-based region coverage control law ensures the coverage task is achieved until every single stratospheric airship ends up performing station keeping where near the respective global minimum point,in which an innovative solution to the local minimum problem is put forward.The adaptive tracking control law is designed to realize motion control using tracking the desired velocity and angular velocity given by coverage control law,with the consideration of several practical control problems as unknown individual differences and external disturbances.To save resources,the combined self-/event-triggered mechanism designed therein significantly reduces the times of state information transmission and control law calculation.The effectiveness of the proposed control framework is verified through simulations.
文摘William Wordsworth is best known for his poem,The Daffodils.This paper traces the origin of daffodils in Greek mythology,the four important facts in Wordsworth’s life and the poetic theories implied in his poem The Daffodils.The most important part shall be the appreciation and analysis of the poem.During this course,readers may find it difficult to understand Wordsworth’s poetic theories of Romanticism.However this is made easier by asking and answering questions and by comparing Chinese and English Romantic poems.
