Effect of Pelleted Total Mixed Ration on Production Performance and Serum Biochemical Index of Growing Hainan Black Goats

[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.

Effect of Pelleted Total Mixed Ration of Different Particle Sizes on Production Performance and Serum Biochemical Index of Fattening Hainan Black Goats

[Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic backgrounds and similar initial weight of(19.02±0.22) kg were randomly divided into three treatments. Goats were fed with Juncao+concentrate(control group), pel-leted total mixed ration I(particle size 6 mm, experimental group I) and II(particle size 5 mm, experimental group II), respectively. The trial lasted 45 d.[Result](1) The final weight and daily gain of experimental group II were significantly higher than those of control group( P<0.05), and the daily gain of experimental group II was also higher than that of experimental group I(P<0.05);the feed intake of experimental group II was markedly higher than that of control group(P<0.05), but was significantly lower than that of experimental group I(P<0.05). The feed/gain ratio of experimental group II was significantly lower than those of control group and experimental group I(P<0.05).(2) The feed gain cost of experimental group II was markedly lower than that of control group(P<0.05), but the gross profit was significantly higher than those of control group and experimental group I(P<0.05).(3) Compared with the control group, the serum total protein content of experimental group II increased markedly( P<0.05) while the urea nitrogen level decreased(P>0.05).[Conclusion] The pelleted total mixed ration with the particle size of 5 mm obtained better fattening ef-fect, and obviously improved the serum total protein level.

The influence of NO and serum endothelins on prognosis of mixed type stroke patients

Objective To explore the influence of NO and serum endothelins change on prognosis of mixed type stroke patients. Method To test amount of ET and NO,to analysis the influence of NO and ET on prognosis using high effective liquid chromatography and radio immunology method and compared with the control group,then treat with t test. Result Concentrations of serum ET and NO of observe groups were apparently larger than that of control group (P<0.01, P<0.05). Conclusion Serum ET and NO increase apparently in mixed type stroke patients,the higher concentration, the severer disease and deformity of mobility.

Effect of Pelleted Total Mixed Ration with Different Concentrate-roughage Ratios on Production Performance and Serum Biochemical Parameters of Fattening Hainan Black Goats

[Objective] This paper was to study effects of pelleted total mixed ration with different concentrate-roughage ratios on production performance and serum biochemical parameters of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic background and similar initial weight of(14.08±0.13) kg were randomly assigned into three treatments. Goats were fed with Juncao + concentrate(control group), pelleted total mixed ration I(concentrate: roughage 50 ∶50, group Ⅰ) and Ⅱ(concentrate: roughage 45∶55, group Ⅱ), respectively. The test lasted 45 d.[Result] The final weight, daily gain and feed intake of rams in experimental group Ⅰ were significantly higher than those of control group and experimental group Ⅱ(P<0.05);the final weight and daily gain of rams in experimental group Ⅰ were also markedly higher than those of experimental group Ⅱ(P<0.05);the feed/gain of rams in experimental group Ⅰ was significantly lower than those in control group and experimental group Ⅱ(P<0.05). The feed gain cost of rams in experimental group Ⅰ was markedly lower than that in experimental group Ⅱ( P<0.05), but was significantly higher than that in experimental group Ⅰ(P<0.05);the gross profit of rams in experimental group Ⅰ was significantly higher than those in control group and experimental group Ⅱ(P<0.05). Compared with control group and experimental group Ⅱ, the serum total protein and glucose lev-els in experimental group Ⅱ increased significantly(P<0.05), but the urea nitrogen content reduced(P>0.05).[Conclusion] When the concentrateroughage ratio of pelleted total mixed ration was 50∶50, the fattening effect of Hainan black goat was better, and the serum total protein and glucose levels were evidently improved.

Clinical features of severe malaria:Protective effect of mixed plasmodial malaria

Objective: To investigate clinically severe malaria patients with Plasmodium falciparum(P. falciparum), Plasmodium vivax(P. vivax) and mixed species infections.Methods: This study was conducted at Dr. Saiful Anwar General Hospital, Malang,Indonesia, from December 2011 to May 2013. Twenty nine patients(mean age of 41 years, 22% female), who suffered from severe malaria according to World Health Organization criteria(major and minor) and other criteria based on previous studies, were selected by consecutive sampling. Blood samples were obtained at admission from peripheral blood for microscopic diagnostic, nested PCR and laboratory examination of blood chemistry. Laboratory results were compared between the groups and correlated to each other.Results: From 29 samples, eight(28%) were diagnosed as P. falciparum mono-infection,12(41%) as P. vivax mono-infection and nine(31%) as mixed infections, confirmed by PCR. Cerebral malaria occurred in P. falciparum or mixed species infection only. Parasitaemia was highest in P. falciparum mono-infection. Mean haemoglobin was significantly lower in P. falciparum than P. vivax infection(P = 0.01). Mean thrombocyte count(77 138/m L) was low in all groups. Mean urea, creatinine, total and direct bilirubin were significantly higher in P. falciparum mono-infection compared to other groups, whereas aspartate aminotransferase and alanine aminotransferase showed no significant differences. Parasitaemia was positively correlated with an increase in urea, creatinine, bilirubin and leucocytosis in all species.Conclusions: Both Plasmodium species can solely or in combination cause severe malaria. Mixed infection was generally more benign than P. falciparum mono-infection and seemed to have some protective effects.

基于不同群体药动学分析工具建立真实世界丙戊酸群体药代动力学模型

目的:基于NONMEM和MONOLIX 2种不同群体药动学分析工具建立真实世界丙戊酸群体药动学模型(PPK),探索可能影响丙戊酸血药浓度的潜在协变量,为癫痫患者使用丙戊酸提供科学依据。方法:回顾性收集2021年1月—2022年12月南京医科大学第二附属医院收治的167例癫痫患者239个丙戊酸稳态血药浓度数据资料,按随机数字表法将其分为模型组(n=111)与验证组(n=56)。2种群体药动学分析工具同时建立适合真实世界的群体药动学模型,采用拟合度优度诊断、可视化预测检验对所得的模型进行内部验证和外部验证,评估模型的预测能力。结果:该群体药动学为一室模型,在使用NONMEM软件建立的最终模型中纳入了联合用药(DDI)-美罗培南和给药方式(ROUTE)作为协变量;而在使用MONOLIX软件建立的最终模型中纳入了联合用药(DDI)-美罗培南和体质量(BW)作为协变量。与此同时,与NONMEM相比较,MONOLIX软件所得到的最终模型具有更好的精密度和准确度。结论:本研究初步构建了适用于癫痫患者给予丙戊酸剂量指导的群体药动学模型。

豆渣混贮对青年期奶牛生长性能、营养物质表观消化率、瘤胃发酵及甲烷排放的影响

本试验以豆渣、麦麸和玉米粉为原料,按鲜重基础8∶1∶1的比例进行混合发酵(混合后水分含量65%),获得豆渣混贮,并用该混贮替代青年期奶牛基础饲粮中的豆粕、麦麸和玉米,探究豆渣混贮对奶牛生长性能、营养物质表观消化率、饲养成本、血清生化指标、瘤胃发酵和甲烷(CH_(4))排放的影响。采用单因素随机试验设计,选取体重[(311.0±29.8)kg]相近的青年期荷斯坦奶牛24头,随机分为对照组和豆渣混贮组,每组12头。结果表明:豆渣混贮具有良好的发酵品质,主要表现为低pH和高乳酸含量。与对照组相比,豆渣混贮组干物质(DM)和有机物(OM)采食量没有显著变化(P>0.05),酸性洗涤纤维(ADF)采食量则显著升高(P<0.05),粗蛋白质(CP)采食量有升高的趋势(P=0.06);豆渣混贮组OM和中性洗涤纤维(NDF)表观消化率显著降低(P<0.05),DM表观消化率呈降低趋势(P=0.07);豆渣混贮组的平均日增重(ADG)显著升高(P<0.05),而饲料费用显著降低(P<0.05);豆渣混贮显著提高了血清中总胆固醇(TC)、甘油三酯(TG)、血氨(BA)和补体4(C4)含量(P<0.05);豆渣混贮对CH4排放量及瘤胃主要发酵参数均无显著影响(P>0.05)。综上所述,豆渣混贮对青年期奶牛生长性能、瘤胃发酵和血清生化指标无不利影响,可作为一种较经济的精料资源用于奶牛生产。

发酵全混合日粮对藏羊营养物质表观消化率、血清生化指标、屠宰性能及肉品质的影响

本文旨在探讨发酵全混合日粮(TMR)对育肥期藏羊营养物质表观消化率、血清生化指标、屠宰性能及肉品质的影响。选用14只5月龄、体重29 kg左右的健康藏羊公羊,随机分成2组,每组7个重复,每个重复1头。对照组饲喂未发酵TMR,试验组饲喂发酵TMR。预试期14 d,正试期70 d。结果表明:1)与对照组相比,试验组藏羊平均日采食量(ADFI)以及中性洗涤纤维(NDF)和半纤维素表观消化率显著提高(P<0.05)。2)与对照组相比,试验组藏羊血清谷丙转氨酶(ALT)活性、甘油三酯(TG)和总胆固醇(TC)含量、总抗氧化能力(T-AOC)以及谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05)。3)与对照组相比,试验组藏羊胴体重和屠宰率显著提高(P<0.05),腹脂重量和指数显著降低(P<0.05)。4)与对照组相比,试验组藏羊背最长肌红度(a^(*))值显著提高(P<0.05),且背最长肌总必需氨基酸含量显著升高(P<0.05),同时背最长肌总非必需氨基酸含量显著下降(P<0.05);其中,试验组背最长肌缬氨酸和异亮氨酸含量显著提高(P<0.05),而背最长肌甲硫氨酸、酪氨酸、天冬氨酸、丝氨酸和谷氨酸含量显著降低(P<0.05)。综上所述,在本试验条件下,对TMR进行发酵处理能够提高藏羊屠宰性能、NDF和半纤维素表观消化率以及机体抗氧化能力,降低腹部脂肪沉积,对背最长肌肉色及营养价值也具有一定的改善作用。

两种不同多个混合纯音频率声治疗慢性主观性耳鸣的疗效分析

目的探讨两种不同多个混合纯音频率声在慢性主观性耳鸣患者中的治疗效果。方法选择2021年4月~2023年4月慢性主观性耳鸣患者100例为对象,用随机数字表法分为两组各50例。两组均采用常规方法治疗,在此基础上,对照组声治疗以耳鸣检测中测得的耳鸣频率为中心频率,由7倍频程的不同频率纯音构成,观察组以耳鸣检测中测得的耳鸣频率为中心频率,由9个1/3频程的不同频率纯音构成,两组均完成3个月治疗,比较两组有效率、耳鸣残障量表(THI)、耳鸣严重程度评估量表(TEQ)、血清细胞黏附因子-1(sVCAM-1)及一氧化氮(NO)水平。结果治疗3个月后,观察组有效率为96.00%,高于对照组84.00%(P<0.05);治疗3个月后,观察组功能性、情感性、灾难性及TEQ评分,sVCAM-1及NO水平低于对照组(P<0.05)。结论由9个1/3频程的不同频率纯音构成的混合音频率声治疗慢性主观性耳鸣有效率较高,能改善患者耳鸣症状,有助于降低sVCAM-1及NO水平。

冷季舍饲条件下两种全混合饲粮对萨藏杂交F1代育成母羊生长性能及血清生化指标的影响

试验旨在探究冷季舍饲条件下两种全混合饲粮对25~35 kg萨藏杂交F1代育成母羊生长性能及血清生化指标的影响。选取体况、表型相近的萨藏杂交F1代育成母羊21只,随机分为3组,每组7个重复,每个重复1只羊。D组(CP 14.26%,ME 12.93 MJ/kg)、G组(CP 15.06%,ME 11.67 MJ/kg)饲喂不同蛋白、能量水平的全混合饲粮,对照组饲喂苜蓿和燕麦干草。预试期7 d,正式试验期60 d。结果显示,G组1~30 d平均日增重、31~60 d增重和平均日增重、1~60 d增重和平均日增重显著高于D组和对照组(P<0.05),31~60 d料重比、1~60 d料重比显著低于D组(P<0.05);D组的1~30 d平均日采食量和料重比、31~60 d、1~60 d平均日采食量显著高于对照组(P<0.05)。G组血清葡萄糖、总胆固醇含量以及乳酸脱氢酶活性显著高于D组和对照组(P<0.05),G组血清谷草转氨酶活性显著高于对照组(P<0.05),D组血清白蛋白和钙含量显著低于对照组(P<0.05),D组血清总胆汁酸和无机磷含量显著高于对照组(P<0.05),D组和G组血清尿素氮含量显著低于对照组(P<0.05)。研究表明,冷季对萨藏杂交F1代育成母羊饲喂全混合饲粮可以提高生长性能,其中G组的饲喂效果较好。

混合人血清诱发豚鼠过敏性休克猝死模型的建立

目的建立多人混合血清致豚鼠过敏性休克死亡动物模型。方法将健康豚鼠18只随机分为致敏组与生理盐水对照组,将致敏组豚鼠右侧后爪掌皮内注射致敏原免疫3周后,诱发过敏性休克,对照组以生理盐水代替致敏原。结果致敏组豚鼠均出现过敏性休克的典型临床表现及体征,放射免疫法检测血清IgE水平显著增高。结论本实验成功地建立了过敏性休克的豚鼠实验动物模型,进一步证明了异体混合血清的高致敏性,为过敏性休克的临床及法医学诊断及治疗研究提供了一个良好的载体。

不同精粗比全混合日粮饲养技术对南方奶牛采食行为、产奶性能和血清游离氨基酸的影响

研究了具有南方奶牛日粮类型特征的全混合日粮饲养技术不同精粗比(T1,60∶40;T2,55∶45;T3,50∶50;T4,45∶55;T5,40∶60)对奶牛的采食行为、产奶性能和血清游离氨基酸的影响。试验结果表明,在全混合日粮饲养技术下,日粮精粗比的增加,奶牛的干物质采食量从T5组的13.57 kg/d显著降低至T1组的12.70 kg/d(P<0.05),咀嚼时间从T5组的786.50 min/d极显著降低至T1组的607.83 min/d(P<0.01);奶牛的产奶量、4%标准奶产量、乳糖、乳总固形物、总非脂固形物产量均随日粮精粗比增加而显著增加(P<0.05),以60%精料添加量的T1组为最高,分别为13.92,13.25,0.65,1.73和1.20 kg/d;但对奶牛的血清游离氨基酸含量无显著影响(P>0.05)。表明在南方奶牛典型日粮采用全混合日粮饲养技术时,日粮精粗比的增加可提高奶牛的产奶性能,降低奶牛的干物质采食量、咀嚼时间,对奶牛的血清游离氨基酸含量没有显著影响。

不同精粗比饲粮对湖羊肉品质、血液指标和肠道发育的影响

为分析等蛋能比下不同精粗比饲粮对湖羊肉品质、血液指标和肠道发育的影响,本研究将40只体重相近、体况良好且健康的湖羊羔羊随机分为4组,每组10只(公母各半),各试验组羊分别饲喂精粗比为70∶30(试验Ⅰ组)、60∶40(试验Ⅱ组)、50∶50(试验Ⅲ组)和40∶60(试验Ⅳ组)的全混合饲粮,试验期为70 d。结果表明,1)正试期14 d时,试验Ⅳ组羊血清尿素氮(urea nitrogen,BUN)含量显著高于试验Ⅰ组(P<0.05),而血糖(glucose,GLU)含量则显著低于第Ⅲ组(P<0.05);正试期35 d时,试验Ⅳ组羊血清BUN和总胆固醇(total cholesterol,TC)含量分别极显著(P<0.01)和显著(P<0.05)高于试验Ⅰ组;正试期56 d时,试验Ⅳ组湖羊血清BUN含量分别极显著(P<0.01)和显著(P<0.05)高于试验Ⅰ和Ⅱ组,而血糖含量则相反。2)试验Ⅰ组羊肉熟肉率极显著高于第Ⅲ组(P<0.01),而剪切力和失水率则相反;试验Ⅰ组湖羊腿肌粗脂肪比例显著高于试验Ⅳ组(P<0.05),而肉色黄度则相反。3)试验Ⅳ组湖羊十二指肠绒毛高度和绒毛高度/隐窝深度极显著或显著高于试验Ⅰ和Ⅲ组(P<0.01或P<0.05),试验Ⅲ组湖羊空肠绒毛高度和隐窝高度均显著高于试验Ⅰ(P<0.05),试验Ⅳ组湖羊空肠绒毛高度/隐窝深度显著高于试验Ⅰ(P<0.05)。综上所述,在等蛋能比下,提高饲粮精粗比能够提高湖羊肉品质,但精粗比过高不利肠道的发育,从本研究结果来看,精粗比为60∶40全混合饲粮的效果最佳。

不同精粗比全混合日粮对湖羊生长性能、血清激素浓度和屠宰性能的影响

本试验旨在研究不同精粗比全混合日粮对湖羊生长性能、血清激素浓度和屠宰性能的影响。选择体重相近、体况良好且健康的湖羊羔羊40只,随机分为4组,每组10只(公母各占1/2)。4组试验羊分别饲喂精粗比为70∶30(试验Ⅰ组)、60∶40(试验Ⅱ组)、50∶50(试验Ⅲ组)和40∶60(试验Ⅳ组)的全混合日粮。试验期为70 d,其中预试期为14 d,正试期为56 d。结果显示:1)正试期第14天和第35天,试验Ⅰ组羔羊平均日增重(ADG)极显著高于试验Ⅲ和Ⅳ组(P<0.01);正试期第35天和第56天,试验Ⅱ组羔羊体重(BW)显著高于试验Ⅳ组(P<0.05)。2)正试期第14天,试验Ⅱ组羔羊体斜长以及试验Ⅰ组羔羊胸围和管围均显著高于试验Ⅳ组(P<0.05);正试期第35天,试验Ⅰ组羔羊胸围和管围均显著高于试验Ⅳ组(P<0.05),试验Ⅰ组羔羊血清瘦素(LEP)浓度显著高于试验Ⅱ组(P<0.05);正试期第56天,试验Ⅰ组羔羊血清生长激素(GH)和四碘甲状腺原氨酸(T4)浓度显著高于试验Ⅳ组(P<0.05),试验Ⅱ组羔羊血清三碘甲状腺原氨酸(T3)浓度显著高于试验Ⅲ和Ⅳ组(P<0.05)。3)试验Ⅱ组羔羊宰前活重、胴体重、屠宰率、后腿重和背膘厚均极显著高于试验Ⅳ组(P<0.01),试验Ⅱ组羔羊肋肉厚、心脏和肝脏重量均显著高于试验Ⅳ组(P<0.05)。综上所述,在本试验条件下,育肥湖羊饲喂精粗比为60∶40的全混合日粮可获得最佳的生长性能和屠宰性能。

STD四种病原体抗体检测的临床应用

目的 检测血清中UU Ab、MH Ab、CT Ab和NG Ab ,观察对NGU诊断的临床意义。方法 采用免疫金标渗滤法测定 2 76例临床有尿道感染症状患者的血清。结果 4种病原体抗体单项阳性检出率 :UU Ab为 2 3 .19% ,MH Ab为 2 .5 4% ,CT Ab为 7.97% ,NG Ab为 1.0 9%。多项混合总阳性检出率为 44 .2 0 %。结论 4种病原体抗体阳性检出为泌尿系感染的病因学诊断和评价病情提供了实验室依据。

花生藤替代颗粒型全混合日粮中苜蓿对湖北黑头羊生长性能、瘤胃发酵及血清生化指标的影响

本试验旨在研究花生藤替代颗粒型全混合日粮中苜蓿对湖北黑头羊生长性能、瘤胃发酵和血清生化指标的影响,从而为黑头羊育肥过程中合理使用粗饲料提供依据。选取30只体重[(20.46±3.23) kg]接近、体况良好的8月龄湖北黑头山羊羯羊,随机分为3组,每组10只,分别饲喂以花生藤(Ⅰ组)、苜蓿(Ⅱ组)和苜蓿+花生藤(Ⅲ组)为粗饲料来源的颗粒型全混合日粮,试验期60 d,其中预试期10 d,正试期50 d。试验结束后称重,并采集瘤胃液和血液样品,分别测定瘤胃液挥发性脂肪酸、氨态氮浓度以及血清总蛋白(TP)、白蛋白(ALB)、尿素氮(UN)、葡萄糖(GLU)含量等指标。结果显示:1)与Ⅰ组相比,Ⅱ组和Ⅲ组黑头羊的平均日增重(ADG)极显著增加(P<0.01),料重比(F/G)极显著降低(P<0.01);2)各组黑头羊的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)表观消化率无显著差异(P>0.05),但Ⅱ组和Ⅲ组在数值上均较Ⅰ组有不同程度的提高;3)Ⅱ组和Ⅲ组黑头羊瘤胃液乙酸、总挥发性脂肪酸浓度显著高于Ⅰ组(P<0.05);4)Ⅱ组和Ⅲ组黑头羊血清ALB含量和白球比(A/G)分别极显著(P<0.01)和显著(P<0.05)高于Ⅰ组,Ⅲ组血清UN、高密度脂蛋白胆固醇(HDL-C)含量以及肌酸激酶(CK)活性显著高于Ⅱ组(P<0.05),其他血清生化指标各组之间无显著差异(P>0.05)。由此得出,在能量和蛋白质水平相当的条件下,单纯以花生藤作为颗粒型全混合日粮的粗饲料来源会降低湖北黑头羊的ADG,提高F/G,降低瘤胃液总挥发性脂肪酸和乙酸浓度;以花生藤和苜蓿组合作为颗粒型全混合日粮的粗饲料来源,则湖北黑头羊可获得与以苜蓿为粗饲料来源时相当的养殖效果。

三种免疫血清制备方法的比较

目的 :比较评价三种兔抗人全血清制备的方法 ,分析各种方法的优缺点及其适用范围 ,找到一种操作简便效果理想同时又节省抗原的方法。方法 :12只成年雄性家兔随机分三组 ,分别制订不同的免疫方案进行免疫 ,获取兔抗人全血清。结果 :混合免疫法不仅适用于抗原较少的抗血清的制备 ,而且操作方法简便 ,效果理想。结论 :混合免疫法较稳定、免疫效果较好。

日粮中添加不同比例木薯茎叶对海南黑山羊生长性能、血清生化指标和养分表观消化率的影响

试验旨在研究将晒干的木薯茎叶以不同比例与精料混合制成的全混合日粮对海南黑山羊生长性能、血清生化指标和养分表观消化率的影响。选择20只海南黑山羊,分成4组(Ⅰ、Ⅱ、Ⅲ、Ⅳ组),每组5只羊(3公2母),Ⅰ、Ⅱ、Ⅲ、Ⅳ组的精料与木薯茎叶比例分别为3∶7、4∶6、5∶5、6∶4,试验期60d。结果显示,Ⅲ组海南黑山羊的平均日增重(ADG)、平均日采食量(ADFI)均高于其他3组,且Ⅲ组ADG显著高于Ⅰ组(P<0.05);除了Ⅰ组谷丙转氨酶(ALT)显著高于Ⅳ组(P<0.05)外,其他血清生化指标在各组间差异均不显著(P>0.05);Ⅲ组的饲料养分表观消化率均最高,且均呈Ⅰ组到Ⅲ组逐渐升高,Ⅳ组降低的趋势。综合以上结果,海南黑山羊育肥羊日粮中木薯茎叶与精料的适宜比例为5∶5,在此基础上,也可适当降低精料含量,增加木薯茎叶含量,以达到降低饲养成本的目的,但建议木薯茎叶添加比例不宜超过60%。

己烯雌酚人工抗原的合成及其抗体制备

以己烯雌酚 (DES)、琥珀酸酐和牛血清白蛋白 (BSA)等为主要原料 ,采用混合酸酐反应 ,首先合成己烯雌酚半琥珀酸酯 (DES- HS) ,经质谱分析鉴定后 ,再将 DES- HS结合到 BSA上 ,并用 SDS- PAGE对 DES- HS- BSA进行测定。以DES- HS- BSA免疫家兔 6次 ,用琼脂双扩散试验测定抗体。试验结果表明 ,高分辨质谱测得合成的 DES- HS分子量为36 8;BSA- HS- DES的 SDS- PAGE图谱经相关软件分析表明 ,单个 BSA上结合有 32个 DES- HS;琼脂扩散试验结果证实 ,免疫兔血清中含有抗 DES的抗体。本试验为下一步研制 DES残留免疫检测试剂盒及抗

混合谷物粉代餐对肥胖患者血脂变化的影响

目的研究以大豆、燕麦、亚麻籽为主的混合谷物粉作为膳食代餐对肥胖患者血脂变化的影响。方法选取本院体检中心100名男性肥胖患者随机分为观察组和对照组,每组50例。观察组给予每日混合谷物粉(大豆、燕麦、亚麻籽等)两包,替代早餐服用,能量为180 k Cal,50例对照组仅给予正常早餐饮食,研究对象于治疗前测定身高、体重和腰臀比,检测血脂水平,包括三酰甘油(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。疗程为3个月,之后重新测定患者体重及血脂水平。结果服用前,观察组和对照组身高、体重、腰臀比和血脂水平比较差异均无统计学意义(P>0.05)。通过3个月的代餐膳食和饮食控制后,观察组BMI、TG、TC和LDL-C低于对照组和服用前,差异均有统计学意义(P<0.05)。对照组干预前后以上各指标比较差异均无统计学意义(P>0.05)。结论混合谷物粉膳食作为一种高纤维低血糖指数代餐,对肥胖患者起到降低体重、血脂的作用。