The aim of this study was to increase the precision of assessment of the number of round cells observed in the per- oxidase test for detection of seminal leukocytes （granulocytes）. The dilution of semen was reduced ...The aim of this study was to increase the precision of assessment of the number of round cells observed in the per- oxidase test for detection of seminal leukocytes （granulocytes）. The dilution of semen was reduced and the volume of suspension examined was increased for semen samples containing between 0.6 and 6 million round cells per mL. A 1 ＋ 5 （1：6） dilution was compatible with measurable peroxidase activity and a sufficiently clear background for cell assessment. At this dilution, and with assessment of all 18 grids on both sides of the Neubauer-improved counting chamber, only three of the 10 samples （nominal cell concentrations of 1.9 ×10^-6-3.3×10^-6 mL-1） presented 400 round cells or more. As lower seminal dilutions were incompatible with easy detection of round cells or their peroxidase reaction product, it was not pos- sible to provide precise measurements （sampling error 5%） of the suggested lower reference limit of 1 ×10^-6 cells per mL. The results indicate that this poor precision of measuring 1×10^-6 round cells per mL could explain the discrepant reports on the acceptability of the cut-off values for leukocytospermia. Such reference limits need to be established with statistically sound methods.展开更多
目的探讨精液中圆形细胞对宫腔内人工受精(IUI)妊娠率的影响。方法分析2012年1月-2012年12月在我中心行IUI治疗的不孕患者精液情况1293个治疗周期,其中选择标准是处理前的精液液化时间≤60mim,处理后活动精子总数(PTMS)>5×106/m...目的探讨精液中圆形细胞对宫腔内人工受精(IUI)妊娠率的影响。方法分析2012年1月-2012年12月在我中心行IUI治疗的不孕患者精液情况1293个治疗周期,其中选择标准是处理前的精液液化时间≤60mim,处理后活动精子总数(PTMS)>5×106/ml的患者,根据处理前精液中圆形细胞浓度分成3组:Ⅰ组,≤1×106/ml;Ⅱ组,>1×106/ml,≤5×106/ml;Ⅲ组>5×106/ml。比较各组间的精液参数和IUI妊娠率。结果 1293个IUI周期共获得181例妊娠,总周期妊娠率是14.00%。精液中圆细胞浓度大于5×106/ml组妊娠率极显著低于其它两组,(0VS13.4%;0 VS 27.86%,P<0.01)。而在流产率组Ⅰ组Ⅱ均差异不显著(P>0.05)。结论在IUI治疗周期中处理前精液中圆细胞浓度大于5×106/ml时可能会是影响妊娠率的重要因素。展开更多
文摘The aim of this study was to increase the precision of assessment of the number of round cells observed in the per- oxidase test for detection of seminal leukocytes （granulocytes）. The dilution of semen was reduced and the volume of suspension examined was increased for semen samples containing between 0.6 and 6 million round cells per mL. A 1 ＋ 5 （1：6） dilution was compatible with measurable peroxidase activity and a sufficiently clear background for cell assessment. At this dilution, and with assessment of all 18 grids on both sides of the Neubauer-improved counting chamber, only three of the 10 samples （nominal cell concentrations of 1.9 ×10^-6-3.3×10^-6 mL-1） presented 400 round cells or more. As lower seminal dilutions were incompatible with easy detection of round cells or their peroxidase reaction product, it was not pos- sible to provide precise measurements （sampling error 5%） of the suggested lower reference limit of 1 ×10^-6 cells per mL. The results indicate that this poor precision of measuring 1×10^-6 round cells per mL could explain the discrepant reports on the acceptability of the cut-off values for leukocytospermia. Such reference limits need to be established with statistically sound methods.
文摘目的探讨精液中圆形细胞对宫腔内人工受精(IUI)妊娠率的影响。方法分析2012年1月-2012年12月在我中心行IUI治疗的不孕患者精液情况1293个治疗周期,其中选择标准是处理前的精液液化时间≤60mim,处理后活动精子总数(PTMS)>5×106/ml的患者,根据处理前精液中圆形细胞浓度分成3组:Ⅰ组,≤1×106/ml;Ⅱ组,>1×106/ml,≤5×106/ml;Ⅲ组>5×106/ml。比较各组间的精液参数和IUI妊娠率。结果 1293个IUI周期共获得181例妊娠,总周期妊娠率是14.00%。精液中圆细胞浓度大于5×106/ml组妊娠率极显著低于其它两组,(0VS13.4%;0 VS 27.86%,P<0.01)。而在流产率组Ⅰ组Ⅱ均差异不显著(P>0.05)。结论在IUI治疗周期中处理前精液中圆细胞浓度大于5×106/ml时可能会是影响妊娠率的重要因素。
