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Quality control of Semen Ziziphi Spinosae standard decoction based on determination of multi-components using TOF-MS/MS and UPLC-PDA technology 被引量:7
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作者 Di Wang Qing Li +5 位作者 Ran Liu Huarong Xu Yidi Yin Yifan Wang Huijia Wang Kaishun Bi 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2019年第6期406-413,共8页
A sensitive,fast and comprehensive method for the quality assessment of Semen Ziziphi Spinosae(SZS)standard decoction with characterization of its chemical components was developed and validated.UPLC-Q/TOF-MS/MS syste... A sensitive,fast and comprehensive method for the quality assessment of Semen Ziziphi Spinosae(SZS)standard decoction with characterization of its chemical components was developed and validated.UPLC-Q/TOF-MS/MS system was used to identify thirty-six chemical components of SZS standard decoction which included nucleosides,phenolic acids,alkaloids,and flavonoids.Furthermore,a UPLC-PDA method was validated to simultaneously determine adenosine,protocatechuic acid,magnoflorine,catechin,protocatechin,vicenin II,spinosin,kaempferol-3-rutinoside,and 6'''-feruloylspinosin which represent four species of characteristic compounds.The qualitative method had been validated according to Chinese Pharmacopoeia(2015 edition)in terms of lineary,repeatability,recovery and stability for all analytes,with the results showing good precision,accuracy and stability.In conclusion,the method using UPLC combined with MS and PDA provided a novel way for the standardization and identification of SZS standard decoction,and also offered a basis for qualitative analysis and quality assessment of the preparations for SZS standard decoction. 展开更多
关键词 IDENTIFICATION semen ziziphi spinosae(SZS) Standard decoction Quality assessment UPLC TOF-MS/MS
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Comparative pharmacokinetics of six major compounds in normal and insomnia rats after oral administration of Ziziphi Spinosae Semen aqueous extract 被引量:16
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作者 Chenhui Du Yan Yan +3 位作者 Chenxi Shen Xiaofang Cui Xiangping Pei Xuemei Qin 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2020年第4期385-395,共11页
Ziziphi Spinosae Semen(ZSS),a traditional Chinese medicine,is used in clinics for the treatment of insomnia in China and other Asian countries.Herein,we described for the first time a comparative pharmacokinetics stud... Ziziphi Spinosae Semen(ZSS),a traditional Chinese medicine,is used in clinics for the treatment of insomnia in China and other Asian countries.Herein,we described for the first time a comparative pharmacokinetics study of the six major compounds of ZSS in normal control(NC)and para-chlorophenylalanine(PCPA)-induced insomnia model(IM)rats that were orally administered the aqueous extract of ZSS.An ultra-high-performance liquid chromatography coupled with quadrupole orbitrap mass(UHPLC-Q-Orbitrap-MS)method was developed and validated for the simultaneous determination of coclaurine,magnoflorine,spinosin,6000-feruloylspinosin,jujuboside A(JuA),and jujuboside B(JuB)in ZSS in rat plasma.The established approach was successfully applied to a comparative pharmacokinetic study.The systemic exposures of spinosin and 6000-feruloylspinosin were decreased in the IM group compared to the NC group,while plasma clearance(CL)was significantly increased.The Tmax values of JuA and JuB in IM rats were significantly lower than those in NC rats.The T1/2 of JuA in the IM group was significantly accelerated.The pharmacokinetic parameters of coclaurine and magnoflorine were not evidently affected between the two groups.These results indicate that the pathological state of insomnia altered the plasma pharmacokinetics of spinosin,6000-feruloylspinosin,JuA,and JuB in the ZSS aqueous extract,providing an experimental basis for the role of ZSS in insomnia treatment.The comparative pharmacokinetics-based UHPLC-Q-Orbitrap-MS using full-scan mode can therefore provide a reliable and suitable means for the screening of potentially effective substances applied as quality markers of ZSS. 展开更多
关键词 ziziphi spinosae semen PHARMACOKINETICS INSOMNIA UHPLC-Q-orbitrap-MS Six compounds
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Quality analysis of commercial samples of Ziziphi spinosae semen(suanzaoren) by means of chromatographic fingerprinting assisted by principal component analysis 被引量:17
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作者 Shuai Sun Hailing Liu +2 位作者 Shunjun Xu Yuzhen Yan Peishan Xie 《Journal of Pharmaceutical Analysis》 CAS 2014年第3期217-222,共6页
Due to the scarcity of resources of Ziziphi spinosae semen (ZSS), many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern ... Due to the scarcity of resources of Ziziphi spinosae semen (ZSS), many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern established in this paper showed three main bioactive compounds in one chromatogram simultaneously. Principal component analysis based on DAD signals could discriminate adulterants and inferiorities. Principal component analysis indicated that all samples could be mainly regrouped into two main clusters according to the first principal component (PC1, redefined as Vicenin II) and the second principal component (PC2, redefined as zizyphusine). PC1 and PC2 could explain 91.42%of the variance. Content of zizyphusine fluctuated more greatly than that of spinosin, and this result was also confirmed by the HPTLC result. Samples with low content of jujubosides and two common adulterants could not be used equivalently with authenticated ones in clinic, while one reference standard extract could substitute the crude drug in pharmaceutical production. Giving special consideration to the well-known bioactive saponins but with low response by end absorption, a fast and cheap HPTLC method for quality control of ZSS was developed and the result obtained was commensurate well with that of HPLC analysis. Samples having similar fingerprints to HPTLC common pattern targeting at saponins could be regarded as authenticated ones. This work provided a faster and cheaper way for quality control of ZSS and laid foundation for establishing a more effective quality control method for ZSS. 展开更多
关键词 ADULTERANT Common pattern Principal component analysis Quality control ziziphi spinosae semen
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Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis 被引量:4
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作者 Hongshuai Yang Qiuyi Liu +6 位作者 Weidong Yin Manjiang Xie Xiang Ji Shuangyu Shao Hong Liang Pengfei Tu Qingying Zhang 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2020年第4期252-259,共8页
A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen(ZSS) and Ziziphi Mauritianae Semen(ZMS) was established, and then it was applied to analyze samples collected from differe... A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen(ZSS) and Ziziphi Mauritianae Semen(ZMS) was established, and then it was applied to analyze samples collected from different locations. A total of 12 common fingerprint peaks were designated in ZSS and ZMS, respectively, and five of which were definitely identified. Of these common peaks, 10 peaks were presented in both ZSS and ZMS, whereas two were characteristic for ZSS and ZMS, respectively. Furthermore, the two characteristic peaks of ZMS were definitely assigned as 6′′′-sinapoylspinosin and frangufoline by HPLC guided purification and structural elucidation. The HPLC fingerprint analysis was proved to be useful in identifying and discriminating ZSS and ZMS, which was beneficial for quality control of ZSS and its products. 展开更多
关键词 ziziphi spinosae semen ziziphi Mauritianae semen Ziziphus jujuba var.spinosa ziziphi mauritianae HPLC fingerprint
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酸枣仁、覆盆子对酒精戒断焦虑征的治疗作用 被引量:5
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作者 邢宇双 梁启超 +4 位作者 郑科文 宋小欣 雷涛 吴宜艳 杨志 《实验室科学》 2017年第5期28-31,35,共5页
研究酸枣仁、覆盆子对酒精戒断焦虑症的治疗作用,并探讨其作用机制。研究方法选取健康SD大鼠随机分组,腹腔注射酒精28天,给药治疗3天,最后一次给药1h后,高架十字迷宫测其焦虑行为,HPLC测海马中去甲肾上腺素的含量。结果与模型组相比,大... 研究酸枣仁、覆盆子对酒精戒断焦虑症的治疗作用,并探讨其作用机制。研究方法选取健康SD大鼠随机分组,腹腔注射酒精28天,给药治疗3天,最后一次给药1h后,高架十字迷宫测其焦虑行为,HPLC测海马中去甲肾上腺素的含量。结果与模型组相比,大剂量覆盆子提取物、大剂量炒制酸枣仁醇提物在高架十字迷宫测试中能显著增加其次数、时间(P<0.05),在HPLC检测中可降低海马中去甲肾上腺素含量(P<0.05),且酸枣仁作用更为显著。由此得出结论,覆盆子提取物、炒制酸枣仁醇提物能减轻酒精戒断时大鼠所表现出的焦虑样行为,其抗焦虑症的作用机制可能与降低大鼠海马组织中去甲肾上腺素含量有关。 展开更多
关键词 覆盆子 酸枣仁 酒精戒断 焦虑
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GSZ Formula Enhances Sleep Quality:Exploring Its Active Ingredients and Mechanism Using a Network Medicine Approach
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作者 Airong Ren Mingxuan Ma +5 位作者 Yongyin Liang Yarong Wang Zhengkun Li Yahui Liu Qing Fan Guozhen Cui 《Clinical Complementary Medicine and Pharmacology》 2024年第1期36-43,共8页
Background:Sleep is essential for maintaining human health,and insomnia is a widespread problem.Traditional Chinese medicine(TCM)has been used for centuries to treat sleep disorders,with fewer reported side effects co... Background:Sleep is essential for maintaining human health,and insomnia is a widespread problem.Traditional Chinese medicine(TCM)has been used for centuries to treat sleep disorders,with fewer reported side effects compared to conventional treatments.Objective:This study seeks to investigate the sleep-promoting effects of the GSZ formula,which comprisesγ-aminobutyric acid(GABA),Schisandrae Chinensis Fructus(Wuweizi in Chinese),and Ziziphi Spinosae Semen(Suanzaoren in Chinese).In addition,this study aims to explore the active ingredients and potential mechanisms underlying the sleep-enhancing effects of the formula.Methods:The impact of GSZ on sleep was evaluated using two models,the complete sleep model and the sub-threshold sleep model.Mice were randomly divided into five groups and orally administered GSZ solution(0.33 g/kg/day or 0.99 g/kg/day),positive drug diazepam(2.50 mg/kg)or a control solution for 30 days.Hypno-sis model was established in mice using pentobarbital sodium.Sleep duration and incidence were measured by recording when the righting reflex of mice disappeared for more than 1 min.GABA and dopamine(DA)levels in mouse brain tissue were measured using ELISA kits.The ingredients of the GSZ formula were identified using mass spectrometry,and the targets of these ingredients and disease-related genes were retrieved from public databases.A network medicine approach was used to calculate the shortest path between ingredient targets and disease-related proteins.The expression levels of potential proteins,such as Akt,p-Akt,GSK-3β,and p-GSK-3β,were analyzed using Western blotting based on the predicted results.Results:GSZ significantly prolonged sleep duration and enhanced the sleep rate in mice(P<0.05).Furthermore,it elevated GABA levels and reduced DA levels in the mouse brain(P<0.05).Network medicine analysis suggested that GABA,stearic acid,genistin,and coumestrol may be the most crucial active ingredients for sleep improve-ment.Western blotting analysis demonstrated that GSZ modulated the protein expression levels of p-Akt/Akt and p-GSK-3β/GSK-3β(P<0.05).Conclusion:Our study demonstrated that the GSZ formula could improve sleep,with key ingredients likely being GABA,stearic acid,genistin,and coumestrol.The mechanism might involve the regulation of the Akt/GSK-3βpathway,as revealed by the network medicine analysis and experimental validation.Our current new findings shed light on the potential mechanisms underlying the sleep-enhancing effects of the GSZ formula,which could provide experimental evidence to develop innovative treatments for insomnia. 展开更多
关键词 Sleep quality ziziphi spinosae semen Schisandrae chinensis fructus γ-aminobutyric acid Network medicine
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Simultaneous qualitative identification of all medicinal herbs in Zaoren Anshen capsule by thin-layer chromatography
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作者 Manjiang Xie Jie Wang +2 位作者 Chenguang Dong Pengfei Tu Qingying Zhang 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2022年第7期499-506,共8页
The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TL... The holistic characterization and quality control of all the medicinal herbs of proprietary Chinese medicines(PCMs)are of great significance to ensure their safety,efficacy,and consistency.Thin-layer chromatography(TLC),a simple and classic approach for qualitatively characterizing and examining quality markers of natural products,has been widely used in the characterization and quality control of traditional Chinese medicines.Zaoren Anshen(ZRAS)capsule,prepared from three medicinal herbs of fried Ziziphi Spinosae Semen,Salvia Miltiorrhiza Radix et Rhizoma,and vinegar-processed Schisandrae Chinensis Fructus,is a famous PCM in China for the treatment of insomnia,amnesia,and dizziness in clinical practice.However,no effective method is available so far for simultaneous identification and examination of all the three medicinal herbs of ZRAS capsule.In the present study,we developed a TLC method via twice-development and visualization by UV light or chromogenic agent,which could be used for simultaneous qualitative identification of all the three medicinal herbs of ZRAS capsule in one plate.Moreover,the sample preparation method was optimized.The developed TLC method was rapid,simple,low-cost,and effective,and thus it could be used for quality control of ZRAS capsule. 展开更多
关键词 Zaoren Anshen capsule ziziphi spinosae semen Salviae Miltiorrhizae Radix et Rhizoma Schisandrae Chinensis Fructus Thin-layer chromatography Qualitative identification
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