Objective: To reveal the central mechanism of acupuncture on the central nervous system.Methods: Excitatory amino acids (EAAs) were determined in cerebral cortex, hippocampus and striate body ofleft side by Shimadzu L...Objective: To reveal the central mechanism of acupuncture on the central nervous system.Methods: Excitatory amino acids (EAAs) were determined in cerebral cortex, hippocampus and striate body ofleft side by Shimadzu LC-6A HPLC. Results: Metabolic disorder existed in excitatory amino acids: The contentof Gin, Gin, Asp and Asn in hippocampus and striate body were generally increased (P < 0. 05, or P < 0. 01 ),the content of Asn in cerebral cortex was also increased (P < 0. 01 ). The changes of inhibited amino acids (I-AAs) to certain extent also existed in the three regions but without regularity. Xingnao Kaiqiao (醒脑开窍)acupuncture method could obviously reduce the levels of Gin, Asp, Gin and Asn in hippocampus and striate bodyand the levels of Asn and Gin in cerebral cortex of senescence accelerate mouse P/8 (SAM-P/8). The non-acuPOint acupuncture method did not exert obvious effects on Gln and Asp which was quite different from XingnaoKaiqiao acupuncture effects. Conclusions: (1 ) The hyperrnetabolism of EAAs existed in the brain of SAM-P/8,which could be regarded as the imPOrtant pathological mechanism of disorder in study and memory and cerebralatrophy. (2) Xingnao Kaiqiao acupuncture method could obviously regulate the abnormal metabolism of cerebralEAAs, which could be regarded as one of the mechanisms of acupuncture effects on improving brain function.(3) Acupuncture effect has remarkable specificity of acupoint.展开更多
文摘Objective: To reveal the central mechanism of acupuncture on the central nervous system.Methods: Excitatory amino acids (EAAs) were determined in cerebral cortex, hippocampus and striate body ofleft side by Shimadzu LC-6A HPLC. Results: Metabolic disorder existed in excitatory amino acids: The contentof Gin, Gin, Asp and Asn in hippocampus and striate body were generally increased (P < 0. 05, or P < 0. 01 ),the content of Asn in cerebral cortex was also increased (P < 0. 01 ). The changes of inhibited amino acids (I-AAs) to certain extent also existed in the three regions but without regularity. Xingnao Kaiqiao (醒脑开窍)acupuncture method could obviously reduce the levels of Gin, Asp, Gin and Asn in hippocampus and striate bodyand the levels of Asn and Gin in cerebral cortex of senescence accelerate mouse P/8 (SAM-P/8). The non-acuPOint acupuncture method did not exert obvious effects on Gln and Asp which was quite different from XingnaoKaiqiao acupuncture effects. Conclusions: (1 ) The hyperrnetabolism of EAAs existed in the brain of SAM-P/8,which could be regarded as the imPOrtant pathological mechanism of disorder in study and memory and cerebralatrophy. (2) Xingnao Kaiqiao acupuncture method could obviously regulate the abnormal metabolism of cerebralEAAs, which could be regarded as one of the mechanisms of acupuncture effects on improving brain function.(3) Acupuncture effect has remarkable specificity of acupoint.
