Effects of Periodical Soil Drying and Leaf Water Potential on the Sensitivity of Stomatal Response to Xylem ABA

The study on the changes of stomatal sensitivity in relation to xylem ABA during periodical soil drying and the effect of leaf water status on the stomatal sensitivity has confirmed that xylem ABA concentration is a good indicator of soil water status around roots and the relation between xylem ABA concentration and predawn leaf water potential remained constant during the three consecutive soil drying cycles based on the slopes of the fitted lines. The sensitivity of stomata to xylem ABA increased substantially as the soil drying cycles progressed, and the xylem ABA concentration needed to cause a 50% decrease of stomatal conductance was as low as 550 nmol/L in the next two soil drying cycle, as compared with the 750 nmol/L ABA in the first cycle of soil drying. The results using the split_root system showed that leaf water deficit significantly enhanced the stomatal response to xylem ABA and the xylem ABA concentration needed to cause a 50% decrease in stomatal conductance was 2 to 4 times smaller in the whole_root_drying treatment than those in the semi_root_drying treatment. These results suggested that the sensitivity of stomata to xylem ABA concentration is not a fixed characteristic.

Impact of Climate Change on Maize Potential Productivity and the Potential Productivity Gap in Southwest China

The impact of climate change on maize potential productivity and the potential productivity gap in Southwest China（SWC） are investigated in this paper.We analyze the impact of climate change on the photosynthetic,light-temperature,and climatic potential productivity of maize and their gaps in SWC,by using a crop growth dynamics statistical method.During the maize growing season from 1961 to 2010,minimum temperature increased by 0.20℃ per decade（p 〈 0.01） across SWC.The largest increases in average and minimum temperatures were observed mostly in areas of Yunnan Province.Growing season average sunshine hours decreased by 0.2 h day^（-1） per decade（p 〈 0.01） and total precipitation showed an insignificant decreasing trend across SWC.Photosynthetic potential productivity decreased by 298 kg ha^（-1）per decade（p 〈 0.05）.Both light-temperature and climatic potential productivity decreased（p 〈 0.05） in the northeast of SWC,whereas they increased（p 〈 0.05） in the southwest of SWC.The gap between lighttemperature and climatic potential productivity varied from 12 to 2729 kg ha^（-1）,with the high value areas centered in northern and southwestern SWC.Climatic productivity of these areas reached only 10%-24%of the light-temperature potential productivity,suggesting that there is great potential to increase the maize potential yield by improving water management in these areas.In particular,the gap has become larger in the most recent 10 years.Sensitivity analysis shows that the climatic potential productivity of maize is most sensitive to changes in temperature in SWC.The findings of this study are helpful for quantification of irrigation water requirements so as to achieve maximum yield potentials in SWC.

Development and Application of a Mitochondrial Genetically Encoded Voltage Indicator in Narcosis

Mitochondrial membrane potential(MMP)plays a crucial role in the function of cells and organelles,involving various cellular physiological processes,including energy production,formation of reactive oxygen species(ROS),unfolded protein stress,and cell survival.Currently,there is a lack of genetically encoded fluorescence indicators(GEVIs)for MMP.In our screening of various GEVIs for their potential monitoring MMP,the Accelerated Sensor of Action Potentials(ASAP)demonstrated optimal performance in targeting mitochondria and sensitivity to depolarization in multiple cell types.However,mitochondrial ASAPs also displayed sensitivity to ROS in cardiomyocytes.Therefore,two ASAP mutants resistant to ROS were generated.A double mutant ASAP3-ST exhibited the highest voltage sensitivity but weaker fluorescence.Overall,four GEVIs capable of targeting mitochondria were obtained and named mitochondrial potential indicators 1-4(MPI-1-4).In vivo,fiber photometry experiments utilizing MPI-2 revealed a mitochondrial depolarization during isoflurane-induced narcosis in the M2 cortex.

Adjoint Sensitivity Study on Idealized Explosive Cyclogenesis

The adjoint sensitivity related to explosive cyclogenesis in a conditionally unstable atmosphere is investigated in this study.The PSU/NCAR limited-area,nonhydrostatic primitive equation numerical model MM5 and its adjoint system are employed for numerical simulation and adjoint computation,respectively.To ensure the explosive development of a baroclinic wave,the forecast model is initialized with an idealized condition including an idealized two-dimensional baroclinic jet with a balanced three-dimensional moderateamplitude disturbance,derived from a potential vorticity inversion technique.Firstly,the validity period of the tangent linear model for this idealized baroclinic wave case is discussed,considering different initial moisture distributions and a dry condition.Secondly,the 48-h forecast surface pressure center and the vertical component of the relative vorticity of the cyclone are selected as the response functions for adjoint computation in a dry and moist environment,respectively.The preliminary results show that the validity of the tangent linear assumption for this idealized baroclinic wave case can extend to 48 h with intense moist convection,and the validity period can last even longer in the dry adjoint integration.Adjoint sensitivity analysis indicates that the rapid development of the idealized baroclinic wave is sensitive to the initial wind and temperature perturbations around the steering level in the upstream.Moreover,the moist adjoint sensitivity can capture a secondary high sensitivity center in the upper troposphere,which cannot be depicted in the dry adjoint run.