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NEW ANTIMICROBIAL SENSITIVITY TESTS OF BIOFILM OF STREPTOCOCCUS MUTANS IN ARTIFICIAL MOUTH MODEL
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作者 李鸣宇 汪俊 +1 位作者 刘正 朱彩莲 《Journal of Shanghai Second Medical University(Foreign Language Edition)》 2004年第2期88-91,共4页
Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatograp... Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatography chamber. Using sodium fluoride and Tea polyphenol as antimicrobial agent and Streptococcus mu-tans as target, sensitivity tests were studied. Results The modeling biofilm assay resulted in a MIC of 1. 28mg/ ml for fluoride against S. mutans, which was 32 times the MIC for broth maco-dilution method. The differential resistance of bacteria bioflim to antimicrobial agent relative to planktonic cells was also demonstrated. Conclusion The biofilm artificial mouth model may be useful in oral products test. 展开更多
关键词 biofilm artificial mouth model antimicrobial sensitivity tests
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An Improvement of the Mass Flux Convection Parameterization Scheme and its Sensitivity Tests for Seasonal Prediction over China 被引量:1
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作者 平凡 高守亭 王会军 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2003年第6期978-990,共13页
A modified cumulus parameterization scheme, suitable for use in a seasonal forecast model, is presented. This parameterization scheme is an improvement of the mass flux convection scheme developed by Gregory and Rownt... A modified cumulus parameterization scheme, suitable for use in a seasonal forecast model, is presented. This parameterization scheme is an improvement of the mass flux convection scheme developed by Gregory and Rowntree (1989; 1990). This convection scheme uses a 'bulk' cloud model to present an ensemble of convective clouds, and aims to represent shallow, deep, and mid-level convection. At present, this convection scheme is employed in the NCC T63L20 model (National Climate Center, China Meteorological Administration). Simulation results with this scheme have revealed some deficiencies in the scheme, although to some extent, it improves the accuracy of the simulation. In order to alleviate the deficiencies and reflect the effect of cumulus convection in the actual atmosphere, the scheme is modified and improved. The improvements include (i) the full estimation of the effects of the large-scale convergence in the lower layer upon cumulus convection, (ii) the revision of the initial convective mass flux, and (iii) the regulation of convective-scale downdrafts. A comparison of the results obtained by using the original model and the modified one shows that the improvement and modification of the original convection scheme is successful in simulating the precipitation and general circulation field, because the modified scheme provides a good simulation of the main features of seasonal precipitation in China, and an analysis of the anomaly correlation coefficient between the simulation and the observations confirms the improved results. 展开更多
关键词 cumulus convection parameterization scheme convection heating and moistening numerical simulation sensitivity test
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Application of the extended Fourier amplitude sensitivity testing(FAST)method to inflated,axial stretched,and residually stressed cylinders 被引量:1
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作者 H.ASGHARI H.TOPOL +1 位作者 B.MARKERT J.MERODIO 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2023年第12期2139-2162,共24页
This paper is dedicated to applying the Fourier amplitude sensitivity test(FAST)method to the problem of mixed extension and inflation of a circular cylindrical tube in the presence of residual stresses.The metafuncti... This paper is dedicated to applying the Fourier amplitude sensitivity test(FAST)method to the problem of mixed extension and inflation of a circular cylindrical tube in the presence of residual stresses.The metafunctions and the Ishigami function are considered in the sensitivity analysis(SA).The effects of the input variables on the output variables are investigated,and the most important parameters of the system under the applied pressure and axial force such as the axial stretch and the azimuthal stretch are determined. 展开更多
关键词 sensitivity analysis(SA) Fourier amplitude sensitivity test(FAST)method cylindrical inflation nonlinear elasticity
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THE SENSITIVITY TEST STUDY OF AIR-SEA SURFACE FLUX MODEL 被引量:1
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作者 姚华栋 阎俊岳 +2 位作者 蒋国荣 何金海 吴咏明 《Journal of Tropical Meteorology》 SCIE 2004年第2期201-209,共9页
In this article, a sensitivity test of air-sea surface flux model was carried out with the field observation data of Project “South China Sea Air-Sea Flux Measurement in 2000”. The results show that sensible heat fl... In this article, a sensitivity test of air-sea surface flux model was carried out with the field observation data of Project “South China Sea Air-Sea Flux Measurement in 2000”. The results show that sensible heat fluxes are sensitive to observation errors, increasing the error of model calculation; In contrast, the latent heat flux and momentum flux are not as sensitive to observation errors as the sensible heat, and their calculated results are reliable. The test result also verifies the rationality of the surface flux values calculated and the conclusions can be used to detect errors in observed data. 展开更多
关键词 sea-air interfacial fluxes sensitivity tests sea surface temperature data of gradients
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Methodology Validation of Microbial Limit Test of Bupi Qiangli Ointment
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作者 李江萍 杨亮 蒋玲 《Agricultural Science & Technology》 CAS 2017年第2期365-367,共3页
[Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting i... [Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting in sample recovery test. [Result] The medium dilution method (1:10 test solution, 0.5 ml/plate) could effectively eliminate the inhibition effect of the Bupi Qiangli Ointment, and the recovery of Staphylococcus aureus was greater than 70% in the 3 batches of samples; and the conventional method exhibited the recoveries of E. coil, Bacillus subtilis, Candida albicans and Aspergillus greater than 70% in the 3 batches of samples. [Conclusion] Due to Bupi Qiangli Ointment has strongly antibacterial effect on Staphylococcus au- reus, the medium dilution method was used for bacterial counting, and the conventional method was used for mold and yeast counting; and the conventional method was used for controlled bacterium examination of E. coll. 展开更多
关键词 Bupi Qiangli Ointment microbial limit tests Method validation Medium dilution method
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Isolation and Identification of Pseudomonas solanacearum and Its Drug Sensitivity Test
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作者 Zhu Li Zhang Qiao 《Plant Diseases and Pests》 CAS 2015年第2期23-25,共3页
Ten pathogenic strains were isolated fi-om gingers infected by blast, and were identified by substrate utilization test and biochemical test. The identifica- tion results showed that these ten strains accorded with th... Ten pathogenic strains were isolated fi-om gingers infected by blast, and were identified by substrate utilization test and biochemical test. The identifica- tion results showed that these ten strains accorded with the basic characteristics of Pseudomonas solanacearum. Drug sensitivity test of ten strains was carried out, and prevention agents were screened to provide an experimental basis for the control of ginger blast. 展开更多
关键词 Pseudomonas solanacearum Ginger blast Substrate utilization test Drug sensitivity test
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Drug Sensitivity Test and Regression Verification of Escherichia coli from Rex Rabbit
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作者 Guo Shijin Wang Yanping +4 位作者 Xu Qianqian Zhang Ying Zhang Zhimei Dong Lin Shen Zhiqiang 《Animal Husbandry and Feed Science》 CAS 2017年第2期91-92,107,共3页
[ Objectives] The paper aimed to select drugs reasonably for treatment of rex rabbit colibacillosis, and to isolate the pathogenicity of Escherichia coll. [ Methods ] Pathogen isolation, drug sensitivity test and path... [ Objectives] The paper aimed to select drugs reasonably for treatment of rex rabbit colibacillosis, and to isolate the pathogenicity of Escherichia coll. [ Methods ] Pathogen isolation, drug sensitivity test and pathogen regression test were performed with rex rabbits killed by E. coli in clinic. [ Results] The isolate was E. coli 0-23, susceptible to amikacin and cefotaxime sodium; when the challenge dose was 1.0 mL/rabbit (about one billion E. coli), the test animal would discharge mucous feces. [ Conclusions] The results provided model support for clinical medicine selection against rex rabbit colibacillosis. 展开更多
关键词 COLIBACILLOSIS Drug sensitivity test Regression verification
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Isolation, Identification and Drug Sensitivity Test of a Pathogenic Escherichia coli Strain from Minks with Diarrhea
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作者 Lirong XIAO Qiaoling LI +3 位作者 Qinghui JIA Guisheng GAO Zhaoxing ZHANG Qiumei SHI 《Agricultural Biotechnology》 CAS 2018年第3期81-83,共3页
[Objectives] The study aimed to identify the pathogen that causes diarrhea in minks. [Methods] Liver tissues were aseptically collected from dead minks with diarrhea. By bacterial isolation and culture,morphological o... [Objectives] The study aimed to identify the pathogen that causes diarrhea in minks. [Methods] Liver tissues were aseptically collected from dead minks with diarrhea. By bacterial isolation and culture,morphological observation,biochemical test and pathogenicity test,the isolated strain was identified as pathogenic E. coli. [Results]The pathogen causing diarrhea in minks was confirmed as a pathogenic E. coli strain. Drug sensitivity test indicated that the isolated pathogenic E. coli strain was highly sensitive to ceftazidime,cefotaxime,enrofloxacin,florfenicol and cephradine,moderately sensitive to ampicillin,ciprofloxacin,amikacin,doxycycline,lincomycin and gentamycin,and resistant to amoxycillin,neomycin,spectinomycin,polymyxin and penicillin. [Conclusions] This study provided reference for the prevention and control of abortion in female minks in Qinhuangdao region. 展开更多
关键词 MINK DIARRHEA Pathogenic Escherichia coli Drug sensitivity test
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Validation of Microbial Limit Test Methods of Compound Gangbangui Capsule
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作者 Xiangling QU Xianmei XU +1 位作者 Sibu MA Renhui YANG 《Agricultural Biotechnology》 CAS 2018年第2期105-108,共4页
[Objectives] This study was conducted to establish a method for microbial limit test of Compound Gangbangui Capsule. [Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules,the microbiol... [Objectives] This study was conducted to establish a method for microbial limit test of Compound Gangbangui Capsule. [Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules,the microbiological limits of non-sterile products were verified. [Results]Compound Gangbangui Capsule has a strong inhibitory effect on S. aureus,and the antimicrobial activity was significantly eliminated after increasing the diluent( 1∶ 20); and the recoveries were in the range of 0. 5-2. 0 when the total quantities of aerobic microbes were determined by dilution method( 1∶ 20). When the total quantities of mould and yeast were determined by conventional method( 1∶ 20),the recoveries were from 0. 5 to 2. 0,and Escherichia coli can be detected in experimental groups by the test solution dilution method. [Conclusions]With the plate method,the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent and conventional method,respectively,and control bacteria,E. coli was examined by conventional method. This method has been proved effective for microbial limit test of Compound Gangbangui Capsule,and can effectively control the quality of the preparation. The method is accurate and reliable. 展开更多
关键词 Compound Gangbangui Capsule microbial limit test method Dilution methodHome
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The Effect of the Atmospheric and Cloud Properties on the Retrieval of Fog Microphysics at Night for MSG/SEVIRI: A Sensitivity Test Using LibRadtran
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作者 Aliton Oliveira da Silva Augusto Jose Pereira Filho Hugo Abi Karam 《Atmospheric and Climate Sciences》 2021年第3期441-457,共17页
The simplest way of building a look-up table (LUT) for the retrieval of cloud microphysical properties is to use a standard atmospheric profile and vertically uniform cloud microphysics. Such an assumption has been de... The simplest way of building a look-up table (LUT) for the retrieval of cloud microphysical properties is to use a standard atmospheric profile and vertically uniform cloud microphysics. Such an assumption has been demonstrated to be incoherent with in-cloud observations. This paper aims to show the effect of some atmospheric conditions associated with fog as well as its macro-and microstructure on brightness temperature (BT) for the MSG/ SEVIRI satellite using libRadtran. The sensitivity tests were performed by gradually changing some features from the initial data, such as cloud cover, total water vapor column, thermal inversion intensity, fog depth, fog microstructure, and others. The results revealed that some variables can cause significant variations on BT and, consequently, discrepancies in the retrieval of fog microphysical properties. Also, a variation as high as 0.5<span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"><span style="color:#4F4F4F;font-family:-apple-system, "font-size:16px;white-space:normal;background-color:#FFFFFF;">&#176;</span>C</span><span style="font-family:Verdana;"> was found on BT just by switching uniform to the non-uniform profile of fog microphysics depending on the channel, the droplet size, and optical thickness.</span></span></span></span> 展开更多
关键词 Fog Microphysics MSG/SEVIRI sensitivity test LibRadtran
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Improving quality of life in self-reported gastrointestinal disorders: An open trial of a food elimination diet guided by the ImmunoBloodprint food sensitivity test 被引量:3
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作者 Judi M. Woolger Johanna Lopez +10 位作者 Angelica B. Melillo Eduard Tiozzo Yaima Alonso Soyona Rafatjah Amine Sarabia Susanna M. Leonard Evan G. Long Michael Schoor Jared Tannenbaum Janet Konefal John E. Lewis 《Open Journal of Internal Medicine》 2013年第3期106-113,共8页
Purpose: Irritable bowel syndrome (IBS) and gastrointestinal (GI) disorders are genuine health problems that have serious consequences on a patient’s quality of life (QoL). IBS is a functional disorder characterized ... Purpose: Irritable bowel syndrome (IBS) and gastrointestinal (GI) disorders are genuine health problems that have serious consequences on a patient’s quality of life (QoL). IBS is a functional disorder characterized by chronically recurring symptoms, including abdominal pain or discomfort, altered stool frequency and consistency, and abdominal bloating in the absence of structural or biochemical abnormalities. Studies have indicated that symptoms and disability related to IBS and GI disorders correlate with QoL measures. Additionally, those who respond to therapy have an improvement in QoL. Therefore, we explored an open trial of a food elimination diet guided by the ImmunoBloodprint food sensitivity test and its effect on QoL in patients self-reporting an unresolved GI disorder. Methods: Eighty-four subjects aged 18 and above participated in the study. Subjects eliminated all IgG-mediated reactive foods from their diet for 90 days. QoL was assessed with the Irritable Bowel Syndrome-36 (IBS-36) and the Medical Outcomes Study Short Form-36 (SF-36) at baseline and 30-, 60-, and 90-day follow-up. The data were analyzed with linear mixed models. Results: Subjects had statistically significant improvements in all indicators of QoL according to the IBS-36 and SF-36 from baseline to 90- day follow-up. Conclusions: Subjects with unresolved self-reported GI disorders were able to improve all indicators of QoL in response to eliminating IgG- reactive foods from the diet. Given the inconvenience of keeping track of foods and symptoms to eliminate intolerant foods, a food sensitivity test combined with an elimination diet may be an effective method for improving subjective markers of QoL and health. 展开更多
关键词 GI DISORDERS FOOD sensitivity testing Elimination Diet FOOD INTOLERANCE IBS-36 SF-36
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Validation for Microbial Limit Test Method of Compound Yu E Nose Drops
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作者 Renhui YANG Peixue CAO +1 位作者 Zhen ZENG Xiangling QU 《Agricultural Biotechnology》 CAS 2019年第5期125-128,共4页
[Objectives] This study was conducted to establish a method for microbial limit test of Compound Yu E Nose Drops.[Methods] According to the Chinese Pharmacopoei (General Rules in the fourth part of the 2015 edition),t... [Objectives] This study was conducted to establish a method for microbial limit test of Compound Yu E Nose Drops.[Methods] According to the Chinese Pharmacopoei (General Rules in the fourth part of the 2015 edition),the microbial limit test method for Compound Yu E Nose Drops was verified.[Results] Compound Yu E Nose Drops has a strong inhibitory effect on Staphylococcus aureus and Bacillus subtilis,and the inhibitory activity was significantly eliminated after increasing the diluent (1∶ 20).The recoveries were all in the range of 0.5-2.0 when the total quantities of aerobic microbes were determined by the dilution method (1∶ 20).When the total quantities of mould and yeast were determined by the conventional method,the recoveries were both in the range of 0.5-2.0.When examining control bacteria,Escherichia coli,S.aureus and Pseudomonas aeruginosa can all be detected in the test groups by the test liquid dilution method.[Conclusions] For Compound Yu E Nose Drops,the total quantities of aerobic microbe can be counted by the dilution method;the quantities of mould and yeast can be examined by the conventional plate method;and the conventional method can be used for control microbe examination. 展开更多
关键词 COMPOUND Yu E NOSE DROPS microbial LIMIT test DILUTION method
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Validation of Microbial Limit Test Methods of Tongmai Tangyanming Capsule
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作者 Renhui YANG Yali YAO Xunrong ZHOU 《Agricultural Biotechnology》 CAS 2018年第1期104-108,共5页
[ Objectives] This study was conducted to establish a method for microbial limit test of Tongmai Tangyanming Capsule. [ Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules, the microb... [ Objectives] This study was conducted to establish a method for microbial limit test of Tongmai Tangyanming Capsule. [ Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules, the microbiological limits of non-sterile products were verified. [ Results] Tongmai Tangyanming Capsule has a strong inhibitory effect on Bacillus subtilis and Candida albicans. The antimicrobial activity was significantly eliminated after increasing the diluent ( 1 : 20) ; and the recoveries were in the range of 0.5 -2 when the total quantities of aerobic microbes were determined by dilution method (1:20). When the total quantities of mould and yeast were determined by dilution method (1:20), the recoveries were from 0.5 to 2, and Escherichia coli, cholate-tolerant Gram-negative bacteria and Salmonella can be detected by the test solution dilution method. [ Condusions] With the plate method, the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent, and conventional method was used for examination of control bacteria including E. coli, cholatetolerant Gram-negative bacteria and Salmonella. This method has been proved effective for microbial limit test of Tongmai Tangyanming Capsule, and can effectively control the quality of the preparation. The method is accurate and reliable. 展开更多
关键词 Tongmai Tangyanming Capsule microbial limit test method Dilution method
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Establishment of Microbial Limit Test Methods for Two Hospital Preparations
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作者 Renhui YANG Yongmin CHEN +3 位作者 Yufen XIA Yongyue GAO Yuling LUO Xianmei XU 《Agricultural Biotechnology》 CAS 2023年第3期92-94,127,共4页
[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of C... [Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of Chinese Pharmacopoeia, applicability tests were conducted on microbial limit test methods for the above two traditional Chinese medicine preparations by the plate method. [Results] The established methods showed recovery values in the range of 0.5-2.0 for both experimental strains, and the control bacteria could be detected in the experimental group, but not in the negative control group. [Conclusions] The microbial limit test methods were reliable for the two traditional Chinese medicine preparations and could be used for quality control. 展开更多
关键词 Yunpi Granules Bupi Qiangli Paste microbial limit test Method applicability test
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Applicability of Microbial Limit Test Method for Qingyan Zhisou Powder
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作者 Changping TAI Yuanfeng YANG +3 位作者 Yongmin CHEN Yuling LUO Keqin MA Renhui YANG 《Agricultural Biotechnology》 CAS 2023年第2期117-120,共4页
[Objectives]This study was conducted to establish a microbial limit test method for Qingyan Zhisou Powder.[Methods]Applicability tests were carried out according to Microbial Limit Tests of Non-sterile Products,Genera... [Objectives]This study was conducted to establish a microbial limit test method for Qingyan Zhisou Powder.[Methods]Applicability tests were carried out according to Microbial Limit Tests of Non-sterile Products,General Rules 1105-1107 of Part IV of Chinese Pharmacopoeia,2020.[Results]Qingyan Zhisou Powder had a strong bacteriostatic effect on Pseudomonas aeruginosa.After increasing the dilution ratio(1:40),the bacteriostatic effect was obviously eliminated.When the total number of aerobic microbes was determined by the test liquid dilution method(1:40),the recovery values were in the range of 50%-200%;and when using the test liquid(1:10)to determine the total number of mold and yeasts,the recovery ranged from 50%to 200%.Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria were detected by both the routine method and dilution method in experimental groups.[Conclusions]The microbial limit test of Qingyan Zhisou Powder adopted the test liquid dilution method,and the routine method could be used for the test of Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria.The applicability test of the method is feasible,with scientific and accurate results,and the method can effectively control the quality of the preparation and is recommended for popularization. 展开更多
关键词 Qingyan Zhisou Powder microbial limit test Method applicability
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Flow-Slip Stability Behavior of Calcareous Sand Treated by Microbially Induced Carbonate Precipitation Technology
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作者 KOU Hailei HE Xiang +4 位作者 HOU Wangxiang LI Zhendong ZHANG Xixin AN Zhaotun LU Jiaqing 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第5期1381-1389,共9页
Flow-slip damage commonly destabilizes coastal slopes.Finding a slope stabilization method for calcareous sands in the South China Sea is crucial.Microbially induced calcite precipitation is a promising,eco-friendly m... Flow-slip damage commonly destabilizes coastal slopes.Finding a slope stabilization method for calcareous sands in the South China Sea is crucial.Microbially induced calcite precipitation is a promising,eco-friendly method for soil stabilization.This study investigates the effect of microbial treatments,initial relative density,initial cell pressure,and initial stress ratio on the flow-slip stability of calcareous sand specimens by using constant shear drained tests.These tests lay the foundation to study the mechanical instability of sand slopes.Results show that the microbial-treated specimens maintain stable stresses longer,take longer to reach the instability,and withstand larger volumetric strains.Microbial treatment effectively enhances sand stability under constant shear drainage,with improvements amplified by higher initial relative density and initial cell pressure.In addition,a smaller initial stress ratio reduces shear effects on the specimen and increases resistance to flow slides.Microanalysis reveals that the flow-slip stability of calcareous sand slopes is enhanced by contact cementation,particle coating,void filling,and mutual embedment of calcium carbonate crystals. 展开更多
关键词 clcareous sand microbially induced carbonate precipitation constant shear drained tests flow-slip stability
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Enhancing Infection Control:A Comprehensive Analysis of Microbial Testing and Clinical Accuracy Assessment
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作者 Lingfeng Lu 《Proceedings of Anticancer Research》 2023年第6期90-95,共6页
Objective:To analyze the value of microbiological testing in the treatment of urinary tract infections.Methods:A total of 552 patients with urinary tract infections,diagnosed and treated between January and December 2... Objective:To analyze the value of microbiological testing in the treatment of urinary tract infections.Methods:A total of 552 patients with urinary tract infections,diagnosed and treated between January and December 2021,were selected for this study.They were divided into observation and control groups,each consisting of 276 cases,using the random number table method.The observation group underwent microbial testing,while the control group underwent routine examinations to compare the diagnosis rate,overall treatment effectiveness,and infection status between the two groups.Results:The observation group exhibited significantly improved diagnosis rate,treatment effectiveness,and infection status compared to the control group(P<0.05).Conclusion:The application of microbial testing in the treatment of urinary tract infections demonstrates high clinical applicability. 展开更多
关键词 Urinary tract infection microbial testing Application effect
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Inuence of oxytetracycline on the structure and activity of microbial community in wheat rhizosphere soil 被引量:38
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作者 YANG Qingxiang, ZHANG Jing, ZHU Kongfang, ZHANG Hao College of Life Sciences, Henan Normal University, Xinxiang 453007, China. 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第7期954-959,共6页
The microbial community composition in wheat rhizosphere was analyzed by detecting colony forming units (CFUs) in agar plates. The total CFUs in rhizosphere were 1.04×10^9/g soil with 9.0×10^8/g bacteria, ... The microbial community composition in wheat rhizosphere was analyzed by detecting colony forming units (CFUs) in agar plates. The total CFUs in rhizosphere were 1.04×10^9/g soil with 9.0×10^8/g bacteria, 1.37×10^8/g actinomyces and 3.6×10^6/g fungi. The 10 dominant bacteria were isolated from wheat rhizosphere and were grouped into genus Bacillus according to their full length 16S rRNA gene sequences. Although belonging to the same genus, the isolated strains exhibited different sensitivities to oxytetracycline. When a series of the rhizosphere soil was exposed under various concentrations of oxytetracycline, the microbial community structure was highly affected with significant decline of CFUs of bacteria and actinomyces (22.2% and 31.7% at 10 mg/kg antibiotic, respectively). This inhibition was clearly enhanced with the increase exposure dosage of antibiotic and could not be eliminated during 30 d incubation. There was no obvious influence of this treatment on fungi population. Among the four soil enzymes (alkaline phosphatase, acidic phosphatase, dehydrogenase and urease), only alkaline phospbatase was sensitive to oxytetracycline exposure with 41.3% decline of the enzyme activity at 10 mg/kg antibiotic and further decrease of 64.3%-80.8% when the dosage over 30 mg/kg. 展开更多
关键词 wheat rhizosphere microbial community antibiotic sensitivity OXYTETRACYCLINE
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The microbial content of unexpired pasteurized milk from selected supermarkets in a developing country 被引量:3
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作者 Melisa Anderson Patrice Hinds +3 位作者 Stacyann Hurditt Princena Miller Donovan McGrowder Ruby Alexander-Lindo 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2011年第3期205-211,共7页
Objective:To determine the presence and levels of microbes in unexpired pasteurized milk from randomly selected supermarkets in Kingston,Jamaica.Methods:The quantitative study used a stratified random sampling techniq... Objective:To determine the presence and levels of microbes in unexpired pasteurized milk from randomly selected supermarkets in Kingston,Jamaica.Methods:The quantitative study used a stratified random sampling technique in the selection of the 20 representative milk samples from six(6) supermarkets.Microbiological tests such as methylene blue reduction,standard plate count(SPC),coliform plate count(CPC),purity plate culture,gram staining and biochemical tests were performed to examine the microbes in purchased unexpired pasteurized milk.Results:One sample(BCr016) had a pH of 4.0.a rancid odour and curdled appearance.It decolourized within one hour during the methylene blue reduction test and was classified as class 4 milk.Seven of the samples were sterile with no microbe growth on the plate count agar and violet red bile salt agar(VRBA).The milk samples that appeared to be safe for consumption were all 10,11,12 and 13 days before expiration.The VRBA sample BCr016,had a colony count of 13 400 CFU/ mL.There was the presence of Escherichia coli in sample LCr021 which had a standard plate count of 1 580 SPC/mL and a coliform count of 500 CFU/mL.Enterobacter sp.was present in colonies from BCr016 and all the other milk samples.Conclusions:Unacceptable levels of Entembacter spp.and Escherichia coli were found in most of the samples.Effective measures to ensure safe milk for human consumption such as the phosphatase test and methylene blue reduction test should be routinely performed on each batch of milk processed by dairy plants. 展开更多
关键词 microbial content Pasteurized MILK Supermarkets MICROBE MICROBIOLOGICAL test ESCHERICHIA coli ENTEROBACTER spp MILK Dairy plant Developing country
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Dual pH and microbial-sensitive galactosylated polymeric nanocargoes for multi-level targeting to combat ulcerative colitis
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作者 Mahira Zeeshan Qurat Ul Ain +3 位作者 Benno Weigmann Darren Story Bryan R.Smith Hussain Ali 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2023年第4期124-141,共18页
Ulcerative colitis(UC)is a type of inflammatory bowel disease characterized by inflammation,ulcers and irritation of the mucosal lining.Oral drug delivery in UC encounters challenges because of multifaceted barriers.D... Ulcerative colitis(UC)is a type of inflammatory bowel disease characterized by inflammation,ulcers and irritation of the mucosal lining.Oral drug delivery in UC encounters challenges because of multifaceted barriers.Dexamethasone-loaded galactosylated-PLGA/Eudragit S100/pullulan nanocargoes(Dexa-GP/ES/Pu NCs)have been developed with a dual stimuli-sensitive coating responsive to both colonic pH and microbiota,and an underneath galactosylated-PLGA core(GP).The galactose ligand of the GP preferentially binds to the macrophage galactose type-lectin-C(MGL-2)surface receptor.Therefore,both stimuli and ligand-mediated targeting facilitate nanocargoes to deliver Dexa specifically to the colon with enhanced macrophage uptake.Modified emulsion method coupled with a solvent evaporation coating technique was employed to prepare Dexa-GP/ES/Pu NCs.The nanocargoes were tested using in vitro,ex vivo techniques and dextran sodium sulfate(DSS)induced UC model.Prepared nanocargoes had desired physicochemical properties,drug release,cell uptake and cellular viability.Investigations using a DSS-colitis model showed high localization and mitigation of colitis with downregulation of NF-ĸB and COX-2,and restoration of clinical,histopathological,biochemical indices,antioxidant balance,microbial alterations,FTIR spectra,and epithelial junctions’integrity.Thus,Dexa-GP/ES/Pu NCs found to be biocompatible nanocargoes capable of delivering drugs to the inflamed colon with unique targeting properties for prolonged duration. 展开更多
关键词 Galactosylated nanocargoes pH-sensitive drug delivery PULLULAN microbial sensitive Ulcerative colitis Macrophage galactose type-lectin C
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