PP1, PP2A and PP2B, belonging to the PPP family of Ser/Thr protein phosphatases, participate in regulating many important physiological processes, such as cell cycle control, regulation of cell growth and division reg...PP1, PP2A and PP2B, belonging to the PPP family of Ser/Thr protein phosphatases, participate in regulating many important physiological processes, such as cell cycle control, regulation of cell growth and division regulation, etc. The sequence homology between them is relatively high, and ter- tiary structure is conserved. Because of the complexity of the structure of PP2A and the diversity of its regulatory subunits, its structure is less well known than those of PP1 and PP2B. The PP2A holoen- zyme consists of a heterodimeric core enzyme, comprising a scaffolding subunit and a catalytic sub- unit, as well as a variable regulatory subunit. In this study, the subunit compositions, similarities and differences between the Ser/Thr protein phsphatases structures are summarized.展开更多
前期研究发现烟草中一个壳寡糖诱导的Ser/Thr蛋白激酶(Oligochitosan induced protein kinase,OIPK)在壳寡糖诱导的烟草抗性反应中起着重要作用.目前在烟草中发现OIPK基因的两个选择性剪接体:OIPKL和OIPKS,其中OIPKL是3个选择性剪接体...前期研究发现烟草中一个壳寡糖诱导的Ser/Thr蛋白激酶(Oligochitosan induced protein kinase,OIPK)在壳寡糖诱导的烟草抗性反应中起着重要作用.目前在烟草中发现OIPK基因的两个选择性剪接体:OIPKL和OIPKS,其中OIPKL是3个选择性剪接体中序列最长的.为进一步研究OIPK在壳寡糖诱导植物抗性中起的作用,本研究采用酵母双杂交方法,以pGBKT7-OIPKL为诱饵质粒,筛选构建在pACT2载体上的烟草cDNA文库,寻找OIPKL的相互作用蛋白.结果表明OIPKL蛋白在酵母中可以和烟草的S-腺苷甲硫氨酸合成酶、泛素激活酶E1、丙酮酸激酶、过氧化物酶相互作用.实验结果为研究壳寡糖诱导抗性机理提供了一定依据.展开更多
基金the National Basic Research Program of China (973) (Grant No. 2004CB719906)the National Natural Science Foundation of China (30470393)
文摘PP1, PP2A and PP2B, belonging to the PPP family of Ser/Thr protein phosphatases, participate in regulating many important physiological processes, such as cell cycle control, regulation of cell growth and division regulation, etc. The sequence homology between them is relatively high, and ter- tiary structure is conserved. Because of the complexity of the structure of PP2A and the diversity of its regulatory subunits, its structure is less well known than those of PP1 and PP2B. The PP2A holoen- zyme consists of a heterodimeric core enzyme, comprising a scaffolding subunit and a catalytic sub- unit, as well as a variable regulatory subunit. In this study, the subunit compositions, similarities and differences between the Ser/Thr protein phsphatases structures are summarized.
文摘前期研究发现烟草中一个壳寡糖诱导的Ser/Thr蛋白激酶(Oligochitosan induced protein kinase,OIPK)在壳寡糖诱导的烟草抗性反应中起着重要作用.目前在烟草中发现OIPK基因的两个选择性剪接体:OIPKL和OIPKS,其中OIPKL是3个选择性剪接体中序列最长的.为进一步研究OIPK在壳寡糖诱导植物抗性中起的作用,本研究采用酵母双杂交方法,以pGBKT7-OIPKL为诱饵质粒,筛选构建在pACT2载体上的烟草cDNA文库,寻找OIPKL的相互作用蛋白.结果表明OIPKL蛋白在酵母中可以和烟草的S-腺苷甲硫氨酸合成酶、泛素激活酶E1、丙酮酸激酶、过氧化物酶相互作用.实验结果为研究壳寡糖诱导抗性机理提供了一定依据.
