Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of t...Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of this special phenomenon for the sericin silkworm were analyzed in order to explore the utilization clues in the production of natural sericin and product development.展开更多
Sericin is a major silk protein, and recent studies have shown that sericin has numerous physiological activities against cultured cells. For example, it accelerates mammalian cell proliferation and protects insect ce...Sericin is a major silk protein, and recent studies have shown that sericin has numerous physiological activities against cultured cells. For example, it accelerates mammalian cell proliferation and protects insect cells against cell death. In this study, we examined the activities of sericin prepared from the wild silkworm, Cricula trifenestrata. The results presented here indicated that C. trifenestrata sericin has anti-proliferative activity against feline kidney cells, while sericin from Bombyx mori promoted cell proliferation. The components of the sericin preparation that played a role in inhibition of proliferation were analyzed by fractionation through partition chromatography (Folch method) and reversed-phase chromatography. Our analysis showed that at least two components in the C. trifenestrata sericin preparation promoted the anti-proliferative activity in an additive or even synergistic manner. Therefore, differences in components between insect species may contribute to different activities against cultured cells.展开更多
文摘Sericin silkworm is a kind of gene mutant silkworm that can only synthesis and secrete sericin protein instead of fibroin protein. In this study, according to the knowledge of physiology and heredity, the reasons of this special phenomenon for the sericin silkworm were analyzed in order to explore the utilization clues in the production of natural sericin and product development.
文摘Sericin is a major silk protein, and recent studies have shown that sericin has numerous physiological activities against cultured cells. For example, it accelerates mammalian cell proliferation and protects insect cells against cell death. In this study, we examined the activities of sericin prepared from the wild silkworm, Cricula trifenestrata. The results presented here indicated that C. trifenestrata sericin has anti-proliferative activity against feline kidney cells, while sericin from Bombyx mori promoted cell proliferation. The components of the sericin preparation that played a role in inhibition of proliferation were analyzed by fractionation through partition chromatography (Folch method) and reversed-phase chromatography. Our analysis showed that at least two components in the C. trifenestrata sericin preparation promoted the anti-proliferative activity in an additive or even synergistic manner. Therefore, differences in components between insect species may contribute to different activities against cultured cells.
