Effects of lonicera granules + symptomatic treatment on serum indexes and immune function in children with hand-foot-and-mouth disease

Objective:To investigate the effects of lonicera granules + symptomatic treatment on serum indexes and immune function in children with hand-foot-and-mouth disease.Methods: A total of 120 children with HFMD who were treated between April 2016 and September 2017 were divided into the control group (n=60) and lonicera granules group (n=60) by random number table method. Control group received clinical symptomatic treatment for hand-foot-mouth disease, lonicera granules group received symptomatic treatment combined with lonicera granules therapy, and the treatment lasted for 7 d. The differences in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin were compared between the two groups before treatment and after 7 d of treatment.Results: Before treatment, there was no statistically significant difference in serum levels of inflammatory factors, myocardial enzyme spectrum indexes and immunoglobulin between the two groups. After 7 d of treatment, serum inflammatory cytokines IL-6, IL-10, IL-12 and TNF-α levels of lonicera granules group were lower than those of control group;serum myocardial enzyme spectrum indexesα-HBDH, LDH, AST and cTnⅠ levels were lower than those of control group;serum immunoglobulin IgA, IgG and IgM levels were higher than those of control group.Conclusion: Symptomatic treatment combined with lonicera granules therapy can further inhibit the systemic inflammatory response, protect the myocardial cells and enhance the immunity of children with HFMD.

Intestinal detoxification time of hand-foot-and-mouth disease in children with EV71 infection and the related factors

Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal viruses.Enterovirus 71(EV71)is the primary pathogen that might cause severe symptoms and even death in children with HFMD.This study aimed to investigate the intestinal detoxification time of HFMD children with EV71 infection and its related factors.Methods:Sixty-five HFMD children with EV71 infection were followed up.Their stool samples were collected once every 4 to 7 days.Viral nucleic acids were detected byfl uorescent polymerase chain reaction until the results became negative.The positive rates of viral nucleic acids were analyzed by the Kaplan-Meier method.The Log-rank test and Cox-Mantel test were used to analyze factors affecting the HFMD children with EV71 infection.Results:On the 2nd,4th,6th and 10th week,the positive rates of viral nucleic acids in stool samples of the 65 children were 94.6%,48.1%,17.2%and 0,respectively.Univariate analysis showed that the intestinal detoxification time of the children were related to gender,pre-admission disease course,severity of disease,and use of steroids or gamma globulin(P<0.05).Multivariate analysis showed that the severity of disease was an independent factor affecting the intestinal detoxification time(P<0.05),with a relative risk of 2.418.Conclusions:The longest intestinal detoxification time of HFMD children with EV71 infection was 10 weeks.The severity of disease was an important factor affecting the intestinal detoxification time of HFMD children with EV71 infection.Severe HFMD children with EV71 infection had a longer intestinal detoxification time.

AECOPD病情严重程度与痰中IL-8、TNF-α水平的关系研究

目的探讨AECOPD不同病情严重程度患者痰液中的白介素8(IL-8)、肿瘤坏死因子α(TNF-α)水平的变化。方法选择AECOPD患者并进行病情严重程度分级,收集痰液测定痰液中的IL-8、TNF-α水平,进行统计学分析,以P值<0.05为有统计学意义的标志。结果(1)不同病情严重程度组的IL-8、TNF-α比较的差异具有统计学意义(P<0.05)。(2)病情严重程度分级与IL-8和TNF-α的相关性均具有统计学意义(P<0.05)。结论AECOPD患者病情严重程度不同,其气道炎症程度也不同,且从病情的严重程度大致可推断其气道炎症程度。

CVP监测在重症手足口病患儿抢救中的应用及护理

目的:探讨中心静脉压测定在重症手足口病患儿抢救中的作用。方法:对本科2008年6月~2010年9月共抢救59例EV71患儿中18例监测中心静脉压的患儿,全部采用股静脉穿刺置管的方法,测定中心静脉压,动态监测2 h^10 d。结果:18例监测中心静脉压患儿中痊愈5例,死亡9例,自动出院4例。结论:监测中心静脉压能指导重症手足口病患儿的抢救,纠正重症手足口病患儿内环境紊乱、电解质紊乱,指导扩容,提高抢救成功率,降低病死率。

小麦白粉病不同分级标准的比较研究

本文对４种常用的以株为单位的小麦白粉病成株期分级标准的比较研究表明：４种分级标准的病情指数调查值问，除在病情指数低于６．３时外，均存在显著差异。进一步统计分析表明：不同分级标准的病情指数调查值间分别呈线性关系或幂函数关系。利用数学模型可使按不同标准调查、计算的病指数据相互转换并得以统一，便于各地资料的交流、比较和利用。

我国农村医疗救助的筹资需求与筹资能力研究

利用第三次国家卫生服务调查和民政统计数据对我国农村医疗救助的筹资需求进行测算,测算结果表明:在中长期内,实现农村医疗救助由目前的"大病救助"模式向"综合救助"模式转型,在财政筹资能力上是完全可行的。为保证农村医疗救助的稳步实施,需建立稳定和制度化的医疗救助筹资机制;通过筹资制度的改革,逐步建立一个"中央政府规划和指导、中央和省级财政出资、地方政府实施"的农村医疗救助体系。

浅谈审机辨治

审机辨治关键在于保持证、机、法和方4者的一致性,也就是说,得出的病机结论必须能完整地解释患者的所有证候,治疗法则要完全针对病机,最后,医生的处方要严格遵守治法。这才是正确的诊病过程,只有以病机为核心,才能取得满意的疗效。审机辨治的"辨治"体现在三个方面:一是针对病机确定治则治法。二是根据治则治法选方用药。三是治法方药随着病机的变化而相应改变。此外,审察病机要从患者的实际临床表现出发,千万不能先入为主。病机诊断不是中医治病的唯一凭据,疾病的种类、患者的体质、生存环境等因素也对诊疗有一定影响,临床上"审机辨治"为主、"辨病论治"为辅已成为中医诊病的常规模式。医生在立法处方时,把病机诊断作为首要依据,同时参考病名诊断给予相应的处理。

The Application Research of WBC Morphology Combined with NAP in the HFMD Co-Infection of Children

In this manuscript the authors have studied the application value of White blood cell morphology combined with neutrophils alkaline phosphatase staining integral value in treatment of pediatric HFMD combined with infection. They showed that comparing the NAP positive rate of HFMD co-infection with the NAP positive rate of other three groups respectively—reference group, HFMD group and anti-infection HFMD group—the t value is 25.7964, 28.2326, 28.3696 and the values of P are 0.0000;Comparing the NAP integral value of HFMD co-infection with the NAP integral value of other three groups respectively, which are reference group, HFMD group and anti-infection HFMD, the t value is 35.8687, 36.2664, 36.1374 and the values of P are 0.0000. They concluded that it is useful to apply WBC morphological examination combined with the positive rate and integral value of NAP staining to diagnose and treatment HFMD.

Effects of recombinant human interferon α 2b atomization on serum cytokines and peripheral blood immune cells in children with HFMD

Objective:To study the effects of recombinant human interferon 2b atomization on serum cytokines and peripheral blood immune cells in children with HFMD.Methods: Children who were diagnosed with hand-foot-and-mouth disease in Tongji Hospital? Tongji Medical College Huazhong University of Science & Technology between June 2014 and August 2017 were selected as the research subjects and randomly divided into the interferonα2b group who accepted human recombinant interferonα2b atomization combined with conventional symptomatic treatment and the control group who accepted conventional symptomatic treatment. The contents of cytokines in serum and the contents of immune cells in peripheral blood were determined before treatment as well as 3 days and 5 days after treatment. Results: 3 days and 5 days after treatment, IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of both groups of children were greatly lower than those before treatment whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those before treatment, and IL-6, IL-13, TNF-α, CRP, MCP-1, VCAM-1 and ICAM-1 contents in serum as well as CD19+CD24highCD27+B cell contents in peripheral blood of interferonα2b group were significantly lower than those of control group whereas CD3+CD4+T cell, CD3+CD8+T cell and CD16+CD56+NK cell contents in peripheral blood were higher than those of control group.Conclusion: Recombinant human interferonα2b atomization treatment of HFMD can reduce the inflammatory response and improve the immune response.

A Novel Pharmacophore Model Derived from a Class of Capsid Protein Enterovirus 71 Inhibitors

Capsid protein enterovirus 71 （EV71） is one of the major viruses that cause the severe encephalitis and thus result in a high mortality in children less than 5 years of age.In an effort to discover new potent inhibitors against EV71,a novel three-dimensional pharmacophore model was developed on 24 inhibitors with different molecular structures and bioactivities.The best hypothesis （Hypo1） has a high predictive power and consists of four features,namely,one hydrophobic point （HY） and three hydrogen-bond acceptors （HA）.Two key features of the best Hypo1,HY1 and HA3 match well with an important narrow hydrophobic canyon and with the surface of LYS274 in the target EV71 active site,respectively.The more versatile feature,HA1,is firstly found to be very influential on these compounds’ bioactivities,which may interact with the other side of the active site in the EV71 receptor.The application of the model is successful in predicting the activities of 30 known EV71 inhibitors with a correlation coefficient of 0.831.Furthermore,Hypo1 demonstrates a superior screening capability for retrieving inhibitors from the database with a high enrichment factor of 70.This study provides some important clues in search for more potent inhibitors against EV71 infection.

2002年小麦病虫草大发生特点及原因分析

由于去年秋播至小麦成熟期间 ,特殊的气候条件及栽培因素的影响 ,使连云港市 2 0 0 2年小麦病虫草大发生 。

小柴胡汤与胆腑郁热型鼻渊——熊大经教授鼻病辨治经验之“重肝胆”思想浅析

熊大经教授出身中医世家，对于耳鼻咽喉疾病的治疗有着深刻的体会。鼻渊是耳鼻咽喉科的常见病、多发病，胆腑郁热证是主要症型。本文简要介绍经方小柴胡汤与胆腑郁热型鼻渊的关系和运用。浅谈熊大经教授鼻病辨治经验之“重肝胆”思想及对小柴胡汤的发挥。

Advances in quantitative assessment of parkinsonian motor symptoms with wearable devices

Parkinson's disease(PD)is featured with motor disorder and nonmotor manifestations(Li et al.,2017;Zhang et al.2016) under the pathological change of Substantia Nigra(Dong et al.,2017)and non substantia nigra including medial prefrontal cortex(Li et al.,2017).Its primary motor symptoms include tremor(oscillatory movement),bradykinesia(slowness of motion),rigidity(increment of muscle tone),and postural instability,which seriously affect patients'quality of life(Du and Chen,2017).

西藏大骨节病严重区水文地质调查成果浅析

大骨节病是西藏自治区地方病中的主要病种之一,在全区73个县(市/区)中,患大骨节病县共有37个(其中重病区16个)。本文结合西藏大骨节病严重区水文地质调查项目的实施情况,对已实施的9个重病县区的水文地质调查从病区概况、病情特征、调查成果方面进行了介绍和浅析,通过供水安全示范工程的实施,解决了区内14 475人的安全饮水问题,对区内人民身体素质的提高和促进西藏经济发展、维护社会稳定具有重要的意义。