BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC...BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway.展开更多
BACKGROUND Serpin peptidase inhibitor clade H member 1(SERPINH1)was initially recognized as an oncogene implicated in various human malignancies.Nevertheless,the clinical relevance and functional implications of SERPI...BACKGROUND Serpin peptidase inhibitor clade H member 1(SERPINH1)was initially recognized as an oncogene implicated in various human malignancies.Nevertheless,the clinical relevance and functional implications of SERPINH1 in colorectal cancer(CRC)remain largely elusive.AIM To investigate the effects of SERPINH1 on CRC cells and its specific mechanism.METHODS Quantitative real-time polymerase chain reaction,western blotting analysis,The Cancer Genome Atlas data mining and immunohistochemistry were employed to examine SERPINH1 expression in CRC cell lines and tissues.A series of in-vitro assays were performed to demonstrate the function of SERPINH1 and its possible mechanisms in CRC.RESULTS SERPINH1 demonstrated elevated expression levels in both CRC cells and tissues,manifested at both mRNA and protein tiers.Elevated SERPINH1 levels correlated closely with advanced T stage,lymph node involvement,and distant metastasis,exhibiting a significant association with poorer overall survival among CRC patients.Subsequent investigations unveiled that SERPINH1 overexpression notably bolstered CRC cell proliferation,invasion,and migration in vitro,while conversely,SERPINH1 knockdown elicited the opposite effects.Gene set enrichment analysis underscored a correlation between SERPINH1 upregulation and genes associated with cell cycle regulation.Our findings underscored the capacity of heightened SERPINH1 levels to expedite G1/S phase cell cycle progression via phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin pathway activation,thereby facilitating CRC cell invasion and migration.CONCLUSION These findings imply a crucial involvement of SERPINH1 in the advancement and escalation of CRC,potentially positioning it as a novel candidate for prognostic assessment and therapeutic intervention in CRC management.展开更多
目的 探讨血清Serpin家庭成员1(serpin family A member 1,SERPINA1)、结缔组织生长因子(connective tissue growth factor,CTGF)、总抗氧化能力(total antioxidation capability,T-AOC)在子宫肌瘤患者中的表达情况及其临床意义。方法 ...目的 探讨血清Serpin家庭成员1(serpin family A member 1,SERPINA1)、结缔组织生长因子(connective tissue growth factor,CTGF)、总抗氧化能力(total antioxidation capability,T-AOC)在子宫肌瘤患者中的表达情况及其临床意义。方法 选取子宫肌瘤患者82例纳入子宫肌瘤组,另选取同期健康体检者68例作为健康对照组。比较2组血清SERPINA1、CTGF、T-AOC表达情况,比较不同临床特征子宫肌瘤患者血清SERPINA1、CTGF、T-AOC表达情况,分析血清SERPINA1、CTGF、T-AOC与子宫肌瘤的相关性,分析血清SERPINA1、CTGF、T-AOC检测诊断子宫肌瘤的效能。结果 子宫肌瘤组血清SERPINA1、CTGF水平高于健康对照组,T-AOC水平低于健康对照组(P<0.05);有阴道不规则出血子宫肌瘤患者血清SERPINA1、CTGF水平高于无阴道不规则出血子宫肌瘤患者,T-AOC水平低于无阴道不规则出血子宫肌瘤患者(P<0.05)。Spearman相关系数分析结果显示,血清SERPINA1、CTGF与子宫肌瘤呈明显正相关,T-AOC与子宫肌瘤呈明显负相关(P<0.05)。血清SERPINA1、CTGF、T-AOC联合检测诊断效能明显优于单项检测(P<0.001)。结论 血清SERPINA1、CTGF、T-AOC在子宫肌瘤患者中呈现异常表达,三项指标联合检测诊断子宫肌瘤的效能更高。展开更多
目的分析超声测定子宫动脉血流参数结合潜在标志物血清Serpin家族成员1(SERPINA1)检测在子宫肌瘤诊断中的应用价值。方法选择2018年2月~2022年5月我院收治的67例子宫肌瘤患者作为观察组,103例子宫腺肌病患者作为对照组;对患者行超声检...目的分析超声测定子宫动脉血流参数结合潜在标志物血清Serpin家族成员1(SERPINA1)检测在子宫肌瘤诊断中的应用价值。方法选择2018年2月~2022年5月我院收治的67例子宫肌瘤患者作为观察组,103例子宫腺肌病患者作为对照组;对患者行超声检查记录患者子宫动脉阻力指数(RI)、子宫动脉搏动指数(PI)、子宫动脉收缩期血流速度、子宫动脉舒张期血流速度;检测受试者血清中SERPINA1蛋白水平;采用Logistic回归模型分析存显著性差异各指标联合应用诊断评估模型并绘制ROC曲线分析各指标单独及联合应用对预测子宫肌瘤的价值。结果观察组患者RI低于对照组(0.53±0.13 vs 0.69±0.08),PI高于对照组(1.56±0.21 vs1.25±0.19),差异有统计学意义(P<0.05);观察组患者血中SERPINA1水平高于对照组(138.59±20.28 vs 46.71±4.39),差异有统计学意义(P<0.05);采用子宫动脉血流参数联合SERPINA1对子宫肌瘤患者进行诊断预测模型为Log(P)=0.658×子宫动脉阻力指数+0.617×子宫动脉搏动指数+0.642×SERPINA1+0.809;采用RI、PI、SERPINA1单独应用预测子宫肌瘤的AUC均大于0.85;采用三指标联合应用预测子宫肌瘤的AUC均高于各指标单独应用,差异有统计学意义(P<0.05)。结论采用超声子宫动脉血流参数结合SERPINA1检测可有效诊断和评估子宫肌瘤,具有较高对的临床应用价值。展开更多
人体的抗凝系统主要有抗凝血酶（antithrombin，AT）系统（包括抗凝血酶和肝素，主要抑制具有丝氨酸蛋白酶活性的凝血因子，如：FXa，凝血酶等）和蛋白 C 系统（包括蛋白 C，蛋白 S，血栓调节蛋白，内皮细胞蛋白 C 受体等，主要灭活凝...人体的抗凝系统主要有抗凝血酶（antithrombin，AT）系统（包括抗凝血酶和肝素，主要抑制具有丝氨酸蛋白酶活性的凝血因子，如：FXa，凝血酶等）和蛋白 C 系统（包括蛋白 C，蛋白 S，血栓调节蛋白，内皮细胞蛋白 C 受体等，主要灭活凝血因子Ⅴ和Ⅷ等），而抗凝血酶系统中的抗凝血酶是人体最重要的抗凝因子之一，约占抗凝活性的70％。抗凝血酶是一种丝氨酸蛋白酶（serpins）抑制剂，主要具有抑制丝氨酸蛋白酶活性的凝血酶、FXa 等因子，在人体抗凝系统中发挥着重要作用。展开更多
旨在初步了解丝氨酸蛋白酶抑制剂基因在扩展莫尼茨绦虫生长发育中的作用。采用分子克隆获得扩展莫尼茨绦虫丝氨酸蛋白酶抑制剂serpin基因部分片段,应用MEGA软件对其进行进化分析。同时以beta-Tubulin基因作为内参基因,采用SYBR Green re...旨在初步了解丝氨酸蛋白酶抑制剂基因在扩展莫尼茨绦虫生长发育中的作用。采用分子克隆获得扩展莫尼茨绦虫丝氨酸蛋白酶抑制剂serpin基因部分片段,应用MEGA软件对其进行进化分析。同时以beta-Tubulin基因作为内参基因,采用SYBR Green real-time RT-PCR技术检测该基因在扩展莫尼茨绦虫4个不同发育阶段即头节、幼节、成节、孕节中的表达差异,最终克隆获得749bp的serpin基因片段。进化分析表明,扩展莫尼茨绦虫serpin基因与多方棘球蚴serpin基因有较近的亲缘关系。标准曲线分析显示,serpin和beta-Tubulin基因的Ct值与阳性质粒的浓度均呈良好的线性关系,相关系数均大于0.99,熔解曲线分析表明,产物为特异的单峰,具有较高的特异性。同时试验结果显示,serpin基因在虫体各发育阶段中的表达丰度存在差异,从高到低依次为孕节、头节、成节、幼节。由此初步推测,此基因可能在扩展莫尼茨绦虫虫卵发育为六钩蚴的过程中发挥一定作用。展开更多
文摘BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway.
基金Supported by Ruian Natural Science Foundation,No.MS2021008.
文摘BACKGROUND Serpin peptidase inhibitor clade H member 1(SERPINH1)was initially recognized as an oncogene implicated in various human malignancies.Nevertheless,the clinical relevance and functional implications of SERPINH1 in colorectal cancer(CRC)remain largely elusive.AIM To investigate the effects of SERPINH1 on CRC cells and its specific mechanism.METHODS Quantitative real-time polymerase chain reaction,western blotting analysis,The Cancer Genome Atlas data mining and immunohistochemistry were employed to examine SERPINH1 expression in CRC cell lines and tissues.A series of in-vitro assays were performed to demonstrate the function of SERPINH1 and its possible mechanisms in CRC.RESULTS SERPINH1 demonstrated elevated expression levels in both CRC cells and tissues,manifested at both mRNA and protein tiers.Elevated SERPINH1 levels correlated closely with advanced T stage,lymph node involvement,and distant metastasis,exhibiting a significant association with poorer overall survival among CRC patients.Subsequent investigations unveiled that SERPINH1 overexpression notably bolstered CRC cell proliferation,invasion,and migration in vitro,while conversely,SERPINH1 knockdown elicited the opposite effects.Gene set enrichment analysis underscored a correlation between SERPINH1 upregulation and genes associated with cell cycle regulation.Our findings underscored the capacity of heightened SERPINH1 levels to expedite G1/S phase cell cycle progression via phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin pathway activation,thereby facilitating CRC cell invasion and migration.CONCLUSION These findings imply a crucial involvement of SERPINH1 in the advancement and escalation of CRC,potentially positioning it as a novel candidate for prognostic assessment and therapeutic intervention in CRC management.
文摘目的 探讨血清Serpin家庭成员1(serpin family A member 1,SERPINA1)、结缔组织生长因子(connective tissue growth factor,CTGF)、总抗氧化能力(total antioxidation capability,T-AOC)在子宫肌瘤患者中的表达情况及其临床意义。方法 选取子宫肌瘤患者82例纳入子宫肌瘤组,另选取同期健康体检者68例作为健康对照组。比较2组血清SERPINA1、CTGF、T-AOC表达情况,比较不同临床特征子宫肌瘤患者血清SERPINA1、CTGF、T-AOC表达情况,分析血清SERPINA1、CTGF、T-AOC与子宫肌瘤的相关性,分析血清SERPINA1、CTGF、T-AOC检测诊断子宫肌瘤的效能。结果 子宫肌瘤组血清SERPINA1、CTGF水平高于健康对照组,T-AOC水平低于健康对照组(P<0.05);有阴道不规则出血子宫肌瘤患者血清SERPINA1、CTGF水平高于无阴道不规则出血子宫肌瘤患者,T-AOC水平低于无阴道不规则出血子宫肌瘤患者(P<0.05)。Spearman相关系数分析结果显示,血清SERPINA1、CTGF与子宫肌瘤呈明显正相关,T-AOC与子宫肌瘤呈明显负相关(P<0.05)。血清SERPINA1、CTGF、T-AOC联合检测诊断效能明显优于单项检测(P<0.001)。结论 血清SERPINA1、CTGF、T-AOC在子宫肌瘤患者中呈现异常表达,三项指标联合检测诊断子宫肌瘤的效能更高。
文摘目的分析超声测定子宫动脉血流参数结合潜在标志物血清Serpin家族成员1(SERPINA1)检测在子宫肌瘤诊断中的应用价值。方法选择2018年2月~2022年5月我院收治的67例子宫肌瘤患者作为观察组,103例子宫腺肌病患者作为对照组;对患者行超声检查记录患者子宫动脉阻力指数(RI)、子宫动脉搏动指数(PI)、子宫动脉收缩期血流速度、子宫动脉舒张期血流速度;检测受试者血清中SERPINA1蛋白水平;采用Logistic回归模型分析存显著性差异各指标联合应用诊断评估模型并绘制ROC曲线分析各指标单独及联合应用对预测子宫肌瘤的价值。结果观察组患者RI低于对照组(0.53±0.13 vs 0.69±0.08),PI高于对照组(1.56±0.21 vs1.25±0.19),差异有统计学意义(P<0.05);观察组患者血中SERPINA1水平高于对照组(138.59±20.28 vs 46.71±4.39),差异有统计学意义(P<0.05);采用子宫动脉血流参数联合SERPINA1对子宫肌瘤患者进行诊断预测模型为Log(P)=0.658×子宫动脉阻力指数+0.617×子宫动脉搏动指数+0.642×SERPINA1+0.809;采用RI、PI、SERPINA1单独应用预测子宫肌瘤的AUC均大于0.85;采用三指标联合应用预测子宫肌瘤的AUC均高于各指标单独应用,差异有统计学意义(P<0.05)。结论采用超声子宫动脉血流参数结合SERPINA1检测可有效诊断和评估子宫肌瘤,具有较高对的临床应用价值。
文摘人体的抗凝系统主要有抗凝血酶（antithrombin，AT）系统（包括抗凝血酶和肝素，主要抑制具有丝氨酸蛋白酶活性的凝血因子，如：FXa，凝血酶等）和蛋白 C 系统（包括蛋白 C，蛋白 S，血栓调节蛋白，内皮细胞蛋白 C 受体等，主要灭活凝血因子Ⅴ和Ⅷ等），而抗凝血酶系统中的抗凝血酶是人体最重要的抗凝因子之一，约占抗凝活性的70％。抗凝血酶是一种丝氨酸蛋白酶（serpins）抑制剂，主要具有抑制丝氨酸蛋白酶活性的凝血酶、FXa 等因子，在人体抗凝系统中发挥着重要作用。
文摘旨在初步了解丝氨酸蛋白酶抑制剂基因在扩展莫尼茨绦虫生长发育中的作用。采用分子克隆获得扩展莫尼茨绦虫丝氨酸蛋白酶抑制剂serpin基因部分片段,应用MEGA软件对其进行进化分析。同时以beta-Tubulin基因作为内参基因,采用SYBR Green real-time RT-PCR技术检测该基因在扩展莫尼茨绦虫4个不同发育阶段即头节、幼节、成节、孕节中的表达差异,最终克隆获得749bp的serpin基因片段。进化分析表明,扩展莫尼茨绦虫serpin基因与多方棘球蚴serpin基因有较近的亲缘关系。标准曲线分析显示,serpin和beta-Tubulin基因的Ct值与阳性质粒的浓度均呈良好的线性关系,相关系数均大于0.99,熔解曲线分析表明,产物为特异的单峰,具有较高的特异性。同时试验结果显示,serpin基因在虫体各发育阶段中的表达丰度存在差异,从高到低依次为孕节、头节、成节、幼节。由此初步推测,此基因可能在扩展莫尼茨绦虫虫卵发育为六钩蚴的过程中发挥一定作用。
