Background: Post-menopausal bleeding is a warning sign that accounts for about 5% of all outpatient gynaecologic visits and is a common indication for referral to rapid access clinics because of the fear of underlying...Background: Post-menopausal bleeding is a warning sign that accounts for about 5% of all outpatient gynaecologic visits and is a common indication for referral to rapid access clinics because of the fear of underlying malignancy. Endometrial malignancies differ from other malignancies in that early symptomization is common, allowing early cure. Patients and Methods: During the study period, 100 women with post-menopausal bleeding having inclusion criteria were evaluated in Al Hussein University Hospital. For each patient full history, general, abdominal and pelvic examination was performed. Routine pre-operative investigations were done. Patients were divided into four groups: Group 1 included 29 patients with endometrial polyp. Group 2 included 34 patients with endometrial hyperplasia. Group 3 included 21 patients with atrophic endometrium. Group 4 included 16 patients with endometrial carcinoma. Results: As regards the predictive value of BMI, in the study there was a high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. When discussing the predictive value of blood glucose level, in the study there was a high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. It is worth to mention that the predictive value of endometrial thickness, in the study, was with high statistical significance in comparison between the endometrial carcinoma group and all other benign groups providing the highest specificity and sensitivity. At the last the predictive value of uterine artery velocimetry, in the study, was with high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. Conclusion: BMI, blood glucose level, endometrial thickness and uterine artery velocimetry indices, improve the prediction of endometrial carcinoma in women with post-menopausal bleeding.展开更多
Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis...Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), un-correlated linear discriminant analysis (ULDA), etc. Through the comparisons of the results, it can be found that ULDA and LDA combined with multiple scatter correction (MSC) pretreatment method successfully distinguish the patients of lung cancer and healthy people. The method has academic significance and promising clinical application value.展开更多
The mutation pattern in protein is a very important feature and is studied through various approaches including the study on mutation pattern in domains where amino acids are converted into numbers from letters. In th...The mutation pattern in protein is a very important feature and is studied through various approaches including the study on mutation pattern in domains where amino acids are converted into numbers from letters. In this study, we converted the amino acids in human adrenoleukodystrophy protein with its 128 missense mutations into random domain using the amino-acid pair predictability, and then we studied their mutation patterns. The results show 1) the mutations are more likely to target the amino-acid pairs whose actual frequency is larger than their predicted one, 2) the mutations are more likely to form the amino-acid pairs whose actual frequency is smaller than their predicted frequency, 3) mutations are more likely to occur at unpredictable amino-acid pairs, and 4) mutations have the trend to narrow the difference between predicted and actual frequencies of amino-acid pairs.展开更多
Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may...Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may need many decades to collect, although lysostaphin is so important for clinical therapeutics and drug development. In order not to passively wait for the accumulation of new data, in this study 1) the 23,442 mutations in 1408 proteins from databank were used to determine whether the mutations in lysostaphin follow the general mutation trend obtained from the databank, 2) the amino-acid pair predictability was used to explore the underlined mechanism for lysostaphin mutations, and 3) the amino-acid distribution probability was used to associate the mutation with dysfunction of lysostaphin. The results show that the mutations in lysostaphin follow the general trend of mutations in proteins;the underlined mechanism for mutations in lysostaphin is explainable from a viewpoint of randomness, and a mutation with increased distribution probability would have a larger chance to dysfunction lysostaphin. This study provides useful information for future design of anti-S. aureus drug and enzyme engineering.展开更多
采用核磁共振方法检测肝硬化、肝细胞癌(简称肝癌)患者和健康人血清中代谢物,研究3组血清代谢物组的差异。利用偏最小二乘法-判别式分析(partial least square-discriminant analysis,PLS-DA)对NMR谱数据进行模式识别分析,探讨利用基于1...采用核磁共振方法检测肝硬化、肝细胞癌(简称肝癌)患者和健康人血清中代谢物,研究3组血清代谢物组的差异。利用偏最小二乘法-判别式分析(partial least square-discriminant analysis,PLS-DA)对NMR谱数据进行模式识别分析,探讨利用基于1HNMR代谢组学技术诊断肝癌的可行性。结果表明,与健康人相比,肝硬化、肝癌患者血清中脂质(低密度脂蛋白和极低密度脂蛋白)、胆碱、乙酰乙酸等含量减少,谷氨酰胺、丙酮酸、苯丙氨酸、酪氨酸等含量增加。PLS-DA分析结果显示肝癌患者可与健康人、肝硬化患者鉴别开来,肝癌诊断灵敏度达92.1%,假阳性率为5.7%,优于血清甲胎蛋白(alpha-fetoprotein,AFP)检测。研究结果表明,基于1HNMR代谢组学技术结合PLS-DA的方法具有灵敏、准确、重复性好等优点,有助于肝癌早期诊断。展开更多
文摘Background: Post-menopausal bleeding is a warning sign that accounts for about 5% of all outpatient gynaecologic visits and is a common indication for referral to rapid access clinics because of the fear of underlying malignancy. Endometrial malignancies differ from other malignancies in that early symptomization is common, allowing early cure. Patients and Methods: During the study period, 100 women with post-menopausal bleeding having inclusion criteria were evaluated in Al Hussein University Hospital. For each patient full history, general, abdominal and pelvic examination was performed. Routine pre-operative investigations were done. Patients were divided into four groups: Group 1 included 29 patients with endometrial polyp. Group 2 included 34 patients with endometrial hyperplasia. Group 3 included 21 patients with atrophic endometrium. Group 4 included 16 patients with endometrial carcinoma. Results: As regards the predictive value of BMI, in the study there was a high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. When discussing the predictive value of blood glucose level, in the study there was a high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. It is worth to mention that the predictive value of endometrial thickness, in the study, was with high statistical significance in comparison between the endometrial carcinoma group and all other benign groups providing the highest specificity and sensitivity. At the last the predictive value of uterine artery velocimetry, in the study, was with high statistical significance in comparison between the endometrial carcinoma group and all other benign groups. Conclusion: BMI, blood glucose level, endometrial thickness and uterine artery velocimetry indices, improve the prediction of endometrial carcinoma in women with post-menopausal bleeding.
文摘Raman spectroscopy was used to distinguish the serums from lung cancer patients and healthy people, through spectral pretreatment method combined with pattern recognition methods including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), un-correlated linear discriminant analysis (ULDA), etc. Through the comparisons of the results, it can be found that ULDA and LDA combined with multiple scatter correction (MSC) pretreatment method successfully distinguish the patients of lung cancer and healthy people. The method has academic significance and promising clinical application value.
文摘The mutation pattern in protein is a very important feature and is studied through various approaches including the study on mutation pattern in domains where amino acids are converted into numbers from letters. In this study, we converted the amino acids in human adrenoleukodystrophy protein with its 128 missense mutations into random domain using the amino-acid pair predictability, and then we studied their mutation patterns. The results show 1) the mutations are more likely to target the amino-acid pairs whose actual frequency is larger than their predicted one, 2) the mutations are more likely to form the amino-acid pairs whose actual frequency is smaller than their predicted frequency, 3) mutations are more likely to occur at unpredictable amino-acid pairs, and 4) mutations have the trend to narrow the difference between predicted and actual frequencies of amino-acid pairs.
文摘Lysostaphin is widely used in clinical settings against Staphylococcus aureus, but its mutants can abolish its killing activity. The difficulty in studies of mutations in lysostaphin is the shortage of data, which may need many decades to collect, although lysostaphin is so important for clinical therapeutics and drug development. In order not to passively wait for the accumulation of new data, in this study 1) the 23,442 mutations in 1408 proteins from databank were used to determine whether the mutations in lysostaphin follow the general mutation trend obtained from the databank, 2) the amino-acid pair predictability was used to explore the underlined mechanism for lysostaphin mutations, and 3) the amino-acid distribution probability was used to associate the mutation with dysfunction of lysostaphin. The results show that the mutations in lysostaphin follow the general trend of mutations in proteins;the underlined mechanism for mutations in lysostaphin is explainable from a viewpoint of randomness, and a mutation with increased distribution probability would have a larger chance to dysfunction lysostaphin. This study provides useful information for future design of anti-S. aureus drug and enzyme engineering.
文摘目的:探讨芪参还五胶囊对血管性痴呆(vascular dementia,VD)痰瘀互结型患者认知功能及血清标志物的影响。方法:将痰瘀互结型VD患者80例应用计算机随机数字发生器分为对照组和观察组各40例。对照组应用吡拉西坦片治疗,观察组采用芪参还五胶囊治疗,两组患者均连续服药6周。观察治疗前后两组患者中医证候积分、认知功能[简易智力状态检查量表(Mini-mental state examination,MMSE)]与日常生活能力量表(ability of daily life,ADL)评分、血清神经元特异性烯醇化酶(neuron specific enolase,NSE)、超敏C反应蛋白(high-sensitive C-reactive protein,hs-CRP)、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)、乙酰胆碱(acetylcholine,Ach)、乙酰胆碱酯酶(acetylcholinesterase,AChE)、胆碱乙酰转移酶(choline acetyltransferase,ChAT)水平变化情况及临床疗效。分析血清指标变化与认知功能变化的相关性。结果:治疗后两组患者智能减退、神情呆钝、形盛体胖、呕吐痰涎、唇甲青紫及肌肤甲错症状均较治疗前减轻,观察组效果优于对照组(P<0.05)。总有效率观察组为90%,高于对照组的60%(P<0.05)。治疗后两组患者MMSE、ADL评分均升高(P<0.05),观察组高于对照组(P<0.05);血清NSE、hs-CRP及AchE水平均降低(P<0.05),观察组低于对照组(P<0.05);BDNF及Ach、ChAT水平均升高(P<0.05),观察组高于对照组(P<0.05)。Pearson相关系数分析显示MMSE、ADL评分均与血清Ach、ChAT、BDNF呈正相关,与血清AchE、NSE及hs-CRP呈负相关,且差异具有统计学意义(P<0.05)。结论:芪参还五胶囊能改善VD患者临床症状,调节血清NSE、hs-CRP、BDNF与胆碱能神经递质水平,提高认知功能和日常生活功能,效果优于吡拉西坦片。
文摘采用核磁共振方法检测肝硬化、肝细胞癌(简称肝癌)患者和健康人血清中代谢物,研究3组血清代谢物组的差异。利用偏最小二乘法-判别式分析(partial least square-discriminant analysis,PLS-DA)对NMR谱数据进行模式识别分析,探讨利用基于1HNMR代谢组学技术诊断肝癌的可行性。结果表明,与健康人相比,肝硬化、肝癌患者血清中脂质(低密度脂蛋白和极低密度脂蛋白)、胆碱、乙酰乙酸等含量减少,谷氨酰胺、丙酮酸、苯丙氨酸、酪氨酸等含量增加。PLS-DA分析结果显示肝癌患者可与健康人、肝硬化患者鉴别开来,肝癌诊断灵敏度达92.1%,假阳性率为5.7%,优于血清甲胎蛋白(alpha-fetoprotein,AFP)检测。研究结果表明,基于1HNMR代谢组学技术结合PLS-DA的方法具有灵敏、准确、重复性好等优点,有助于肝癌早期诊断。
