Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through...Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through this method, we can select very complex Chinese medicine components and clarify the effective substances consistent with the main functions, and then separate and purify effective ingredients in traditional Chinese medicine, so as to explain the material basis of the drug effect and prove the rationality and principle of action of Chinese medicine compounds. It can show the multi-component and multi-target characteristic of traditional Chinese medicine, and after analyzing the migrating components of serum, we can explore the direct effective substances that act on the patient’s body, so as to accelerate and accurately complete the in-depth study of the effective substances of Chinese medicine.展开更多
Objective： To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine （CM） of Fuzheng Guben （扶正固本, supporting the healthy energy and strengthening the body＇s r...Objective： To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine （CM） of Fuzheng Guben （扶正固本, supporting the healthy energy and strengthening the body＇s resistance to pathogens）, the effects of Fuzheng Yiliu Decoction （扶正抑瘤方, FYD）, a typical prescription of Fuzheng Guben, on proliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology, respectively. Methods： HepG2 cells were treated with FYD- containing serum or crude FYD extract in vitro. The proliferation rate was determined by methyl thiazolyl tetrazolium （MTT） assay. Cell cycle and apoptosis rate was performed by flow cytometry. And the levels of interleukin-2 （IL-2） and tumor necrosis factor α （TNF-α） in FYD-containing serum were detected by radioimmunoassay. Results： FYD-containing serum remarkably inhibited proliferation and induced apoptosis of hepatoma cells at least by promoting the production of IL-2 and TNF- α in vivo. On the contrary, crude FYD extract promoted the proliferation and did not induce cell apoptosis. Conclusion： The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.展开更多
Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctonus, Rhizoma chuanxiong, Seme...Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctonus, Rhizoma chuanxiong, Semen pruni persicae, and Radix Paeoniae Rubra. After 24 hours of co-cultivation, glutamate （12.5 mM） was added to the culture medium. We found that serum containing Tongqiaohuoxue decoction prevented the increase in reactive oxygen species, and the decreases in superoxide dismutase and Na＋-K＋-ATPase activity, induced by glutamate. It also reduced the concentration of malondialdehyde, enhanced the mitochondrial transmembrane potential, inhibited the elevation of cellular calcium, and decreased phosphorylation of calmodulin-dependent protein kinase I1. Thus, serum containing Tongqiaohuoxue decoction had protective effects on cell proliferation and membrane permeability in glutamate-injured PC12 cells.展开更多
目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给...目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给药组按0.5 g·头-1·d^(-1)剂量通过拌料经口饲喂广藿香中药配方颗粒,连续给药8 d;对照组正常饲喂,不额外给药。用药结束后采集各组动物的血清样品,采用LC-MS/MS技术分析各组样品的血清代谢物,对代谢组数据进行注释并与KEGG、HMDB和Lipidmaps数据库比对,通过偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)、组间聚类、差异代谢物分析和功能富集等生物信息学分析方法,筛选差异代谢生物标志物及其可能的代谢通路。结果在广藿香给药组和对照组的血清样本中共鉴定出443个血清代谢物,筛选出44个差异显著(P<0.05)的代谢物,其中显著上调(P<0.01)的代谢物为肉桂酰甘氨酸、N-苄基-N-异丙基-N'-(4-三氟甲基)苯尿素、亚牛磺酸、D-葡萄糖-6-磷酸、顺-2-癸烯酸、二十碳二烯酸(顺-11,14)、前列腺素A2和10-羟基癸烯酸,显著下调(P<0.01)的代谢物为溶血磷脂酰胆碱22∶5、溶血磷脂酸22∶6和溶血磷脂酸22∶5。差异代谢物主要富集在丙氨酸、天冬氨酸和谷氨酸代谢通路(MapID:map00250),以及牛磺酸和亚牛磺酸代谢通路(MapID:map00430)。结论广藿香能显著影响贵州小型猪的溶血磷脂酸类物质的代谢,可能通过影响天冬氨酸-谷氨酸代谢通路、牛磺酸-亚牛磺酸代谢通路,起到缓解机体氨基酸代谢紊乱、调节炎性反应发生等功能。展开更多
基金Supported by University Continuing Education Teaching Reform Project(2019jxjj67)Provincial quality engineering project of"Traditional Chinese Medicine Technique and Skilled Master Studio"of Anhui Provincial Department of Education in 2020(2020dsgzs23)+3 种基金Key Natural Science Research Project of Higher Learning Institutions in Anhui Province in 2018(KJ2018A0884)Key Natural Science Research Projects of Colleges and Universities in Anhui Province in 2020(KJ2020A1014)Key Project of 2020 Applied Technology Research and Collaborative Innovation Center of Bozhou Vocational and Technical College(BZZX202001)Key Research Project of Bozhou Vocational and Technical College in 2020(BYK2002)。
文摘Serum pharmacochemistry of traditional Chinese medicine has become a more accurate and rapidly developing reasonable way to analyze the effective material basis of traditional Chinese medicine in recent years. Through this method, we can select very complex Chinese medicine components and clarify the effective substances consistent with the main functions, and then separate and purify effective ingredients in traditional Chinese medicine, so as to explain the material basis of the drug effect and prove the rationality and principle of action of Chinese medicine compounds. It can show the multi-component and multi-target characteristic of traditional Chinese medicine, and after analyzing the migrating components of serum, we can explore the direct effective substances that act on the patient’s body, so as to accelerate and accurately complete the in-depth study of the effective substances of Chinese medicine.
基金Supported by International Science Joint Project,the Ministry of Science and Technology of the People's Republic of China(No.2008DFA32200)the National Natural Science Foundation of China(No.81102582)+1 种基金Fujian Province Natural Science Foundation(No.2010J01197)CHEN Ke-ji Integrative Medicine Developmental Foundation(No.CKJ2009002)
文摘Objective： To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine （CM） of Fuzheng Guben （扶正固本, supporting the healthy energy and strengthening the body＇s resistance to pathogens）, the effects of Fuzheng Yiliu Decoction （扶正抑瘤方, FYD）, a typical prescription of Fuzheng Guben, on proliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology, respectively. Methods： HepG2 cells were treated with FYD- containing serum or crude FYD extract in vitro. The proliferation rate was determined by methyl thiazolyl tetrazolium （MTT） assay. Cell cycle and apoptosis rate was performed by flow cytometry. And the levels of interleukin-2 （IL-2） and tumor necrosis factor α （TNF-α） in FYD-containing serum were detected by radioimmunoassay. Results： FYD-containing serum remarkably inhibited proliferation and induced apoptosis of hepatoma cells at least by promoting the production of IL-2 and TNF- α in vivo. On the contrary, crude FYD extract promoted the proliferation and did not induce cell apoptosis. Conclusion： The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.
基金supported by the National Natural Science Foundation of China, No. 30973979the Science-Technology Foundation for Excellent Young Scholar of Anhui Province China, No. 10040606Y17Kanion Innovation Fund of Traditional Chinese Medicine, No. KYCX2010007
文摘Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctonus, Rhizoma chuanxiong, Semen pruni persicae, and Radix Paeoniae Rubra. After 24 hours of co-cultivation, glutamate （12.5 mM） was added to the culture medium. We found that serum containing Tongqiaohuoxue decoction prevented the increase in reactive oxygen species, and the decreases in superoxide dismutase and Na＋-K＋-ATPase activity, induced by glutamate. It also reduced the concentration of malondialdehyde, enhanced the mitochondrial transmembrane potential, inhibited the elevation of cellular calcium, and decreased phosphorylation of calmodulin-dependent protein kinase I1. Thus, serum containing Tongqiaohuoxue decoction had protective effects on cell proliferation and membrane permeability in glutamate-injured PC12 cells.
文摘目的基于液相色谱-串联质谱联用(liquid chromatography-tandem mass spectormetry,LC-MS/MS)技术研究广藿香对贵州小型猪血清代谢的影响,探讨其药理学作用机制。方法将9头贵州小型猪随机分为广藿香给药组(5头)和对照组(4头)。广藿香给药组按0.5 g·头-1·d^(-1)剂量通过拌料经口饲喂广藿香中药配方颗粒,连续给药8 d;对照组正常饲喂,不额外给药。用药结束后采集各组动物的血清样品,采用LC-MS/MS技术分析各组样品的血清代谢物,对代谢组数据进行注释并与KEGG、HMDB和Lipidmaps数据库比对,通过偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)、组间聚类、差异代谢物分析和功能富集等生物信息学分析方法,筛选差异代谢生物标志物及其可能的代谢通路。结果在广藿香给药组和对照组的血清样本中共鉴定出443个血清代谢物,筛选出44个差异显著(P<0.05)的代谢物,其中显著上调(P<0.01)的代谢物为肉桂酰甘氨酸、N-苄基-N-异丙基-N'-(4-三氟甲基)苯尿素、亚牛磺酸、D-葡萄糖-6-磷酸、顺-2-癸烯酸、二十碳二烯酸(顺-11,14)、前列腺素A2和10-羟基癸烯酸,显著下调(P<0.01)的代谢物为溶血磷脂酰胆碱22∶5、溶血磷脂酸22∶6和溶血磷脂酸22∶5。差异代谢物主要富集在丙氨酸、天冬氨酸和谷氨酸代谢通路(MapID:map00250),以及牛磺酸和亚牛磺酸代谢通路(MapID:map00430)。结论广藿香能显著影响贵州小型猪的溶血磷脂酸类物质的代谢,可能通过影响天冬氨酸-谷氨酸代谢通路、牛磺酸-亚牛磺酸代谢通路,起到缓解机体氨基酸代谢紊乱、调节炎性反应发生等功能。
