[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The d...[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.[Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.[Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.展开更多
[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order...[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order to solve the problem of copper pollution. [Method] Setcreasea purpurea Boom was taken as experimental material which enjoys enrichment ability to cupric ion. About 90 fragments of differential expression were obtained by cDNA-AFLP and silver staining technique, among which, 17 fragments were amplified. After purification and identification, sequences of 6 differential fragments were got and used for BLAST X contrast. [Result] Six differential expressed fragments may play roles when Setcreasea purpurea Boom was under copper stress. The homology achieved 49% between differential sequences of E5MG-3 and of Arabidopsis thaliana mRNA （accession numbers： AAM62956.1）, homology was 53% between sequences of E4MB-2 and Solanum tuberosum mRNA （accession numbers： A5A717.1）, and 65% between sequences of E6MG-1 and Colocasia esculenta （L.） Schott mRNA （accession numbers： AAO62313.1）. It can be concluded that differential expressed genes are related to cell signaling, antioxidation, metabolism and protein modification. [Conclusion] The study has laid foundation for further exploration of regulatory network about response of Setcreasea purpurea Boom to copper stress.展开更多
[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods...[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods of water culture, elec- trophoresis and chromatography were used to analyze the molecular weight of the specific protein in the copper hyperaccumulator S. purpurea, as well as its expression time and the minimum copper concentration for the expression. And the specific protein was isolated and purified. [Result] Under copper stress, the minimum concentra- tion of copper to induce the expression of the specific protein from S. purpurea was 50 umol/L, and the expression time of the protein was in the 4th week with the molecular weight of 89.4 kDa. [Conclusion] The results show that the copper tolerance of S. purpurea is closely related with the expression of the specific protein.展开更多
基金Supported by the National Natural Science Foundation(30760021)Natural Science Foundation of Jiangxi Province(0530016)Project of the Education Department of Jiangxi Province(Jiangxi Education&Tech-nology No.[2007]151)~~
文摘[Objective] The aim of this study was to reveal the mechanism of Cu2+ hyper-accumulator in Setcreasea purpurea Boom from the angle of distribution characteristics and binding form of Cu2+ in tissue cells.[Method]The distribution characteristics of Cu2+ in subcells of Setcreasea purpurea Boom was studied by the technique of differential centrifugation,and the binding form of Cu2+ in roots and leaves of Setcreasea purpurea Boom was also investigated by the sequential chemical extraction method and the enzymolysis method.[Result]Cu2+ in roots mainly distributed in the cell wall which is accounting for one third of the Total Cu2+ in roots,while Cu2+ in leaves mainly distributed in the chloroplast which is accounting for a quarter to the Total Cu2+ in leaves.Under the high concentration of Cu2+ or the extended treatment duration,the translocation of Cu2+ in root cells into the cell wall increased but the translocation of Cu2+ in root cells into the plastid decreased,while the translocation of Cu2+ in leaf cells into the chloroplast increased but the translocation of Cu2+ in leaf cells into the cell wall decreased.Cu2+ in leaves was mainly combined with amino acid,small molecular polymeric pigments,protein and polysaccharide,while Cu2+ in roots was mainly combined with cell wall substances such as cellulose and membrane-bound protein.[Conclusion]The distribution characteristics and binding form of Cu2+ in cells is possibly one of the dominant mechanisms for Cu2+ hyper-accumulator in Setcreasea purpurea Boom.
基金Supported by National Natural Science Foundation of China(30760021)Natural Science Foundation of Jiangxi Province(0530016)Project from Education Bureau of Jiangxi Province([2007]151)~~
文摘[Objective] The aim was to explore biological function of related genes in Setcreasea purpurea Boom under copper stress and to study the mechanism of its copper-resistance from standpoint of molecular biology in order to solve the problem of copper pollution. [Method] Setcreasea purpurea Boom was taken as experimental material which enjoys enrichment ability to cupric ion. About 90 fragments of differential expression were obtained by cDNA-AFLP and silver staining technique, among which, 17 fragments were amplified. After purification and identification, sequences of 6 differential fragments were got and used for BLAST X contrast. [Result] Six differential expressed fragments may play roles when Setcreasea purpurea Boom was under copper stress. The homology achieved 49% between differential sequences of E5MG-3 and of Arabidopsis thaliana mRNA （accession numbers： AAM62956.1）, homology was 53% between sequences of E4MB-2 and Solanum tuberosum mRNA （accession numbers： A5A717.1）, and 65% between sequences of E6MG-1 and Colocasia esculenta （L.） Schott mRNA （accession numbers： AAO62313.1）. It can be concluded that differential expressed genes are related to cell signaling, antioxidation, metabolism and protein modification. [Conclusion] The study has laid foundation for further exploration of regulatory network about response of Setcreasea purpurea Boom to copper stress.
基金Supported by the National Natural Science Foundation of China (30760021)the Natural Science Foundation of Jiangxi Province,China (0530016)the Program of Education Bureau of Jiangxi Province,China (ganjiaojizi[2007]No.151)~~
文摘[Objective] This study was to investigate the expression of the specific protein in Setcreasea purpurea Boom under copper stress, with the aim to clarify the copper tolerance mechanism of S. purpurea. [Method] Methods of water culture, elec- trophoresis and chromatography were used to analyze the molecular weight of the specific protein in the copper hyperaccumulator S. purpurea, as well as its expression time and the minimum copper concentration for the expression. And the specific protein was isolated and purified. [Result] Under copper stress, the minimum concentra- tion of copper to induce the expression of the specific protein from S. purpurea was 50 umol/L, and the expression time of the protein was in the 4th week with the molecular weight of 89.4 kDa. [Conclusion] The results show that the copper tolerance of S. purpurea is closely related with the expression of the specific protein.
