[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in...[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.展开更多
基金Supported by Youth Fund Project in Sichuan Province(2008ZQ026-072)Support Science and Technology Project of Sichuan Province(2008FZ0157)~~
文摘[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.
