Sex effect on growth performance and marker-aided sex discrimination of seedlings of Populus deltoides

Sex has a significant effect on various traits among dioecious plants.In this analysis of the sex effect on the radial growth and wood density of numerous 20-yearold trees of Populus deltoides growing in a common garden,male trees performed better than the females in radial growth,but sex did not significantly affect wood density.Growth rate and wood density were weakly negatively correlated.Sex selection is also critical for controlling seed-hair pollution from P.deltoides plantations.However,because the juvenile period of P.deltoides lasts for years,a reliable technique to determine the sex of juveniles has been needed.Here we developed a marker-aided technique to discriminate the sexes of P.deltoides seedlings.This study provides essential information on target traits and a highly desirable genetic toolkit for accelerate breeding programs for this important tree species.

A study of the influence of genetic variance and sex on the density and thickness of the calvarial bone in collaborative cross mice

Background:Bone microarchitecture is affected by multiple genes,each having a small effect on the external appearance.It is thus challenging to characterize the genes and their specific effect on bone thickness and porosity.The purpose of this study was to assess the heritability and the genetic variation effect,as well as the sex effect on the calvarial bone thickness(Ca.Th)and calvarial porosity(%PoV)using the Collaborative Cross(CC)mouse population.Methods:In the study we examined the parietal bones of 56 mice from 9 lines of CC mice.Morphometric parameters were evaluated using microcomputed tomography(μCT)and included Ca.Th and%PoV.We then evaluated heritability,genetic versus environmental variance and the sex effect for these parameters.Results:Our morphometric analysis showed that Ca.Th and%PoV are both significantly different among the CC lines with a broad sense heritability of 0.78 and 0.90,respectively.The sex effect within the lines was significant in line IL111 and showed higher values of Ca.Th and%PoV in females compared to males.In line IL19 there was a borderline sex effect in Ca.Th in which males showed higher values than females.Conclusions:These results stress the complexity of sex and genotype interactions controlling Ca.Th and%PoV,as the skeletal sexual dimorphism was dependent on the genetic background.This study also shows that the CC population is a powerful tool for establishing the genetic effect on these traits.

GENETIC MODELS AND ANALYSIS METHODS FOR SEX-LINKED AND MATERNAL GENE EFFECTS

Genetic models are proposed for analyzing sex-linked and maternal effects as well as autosomal gene effects.For the model with no genotype×environment interaction,the total genetic effect is partitioned into direct additive （A）,direct dominance （D）,sexlinked （L）,maternal additive （Am） and maternal dominance （Dm） genetic components.For the model including genotype×environment interaction （GE）,GE can also be partitioned into components of direct additive by environment interaction （AE）,direct dominance by environment interaction （DE）,sex-linked by environment interaction （LE）,maternal additive by environment interaction （AmE ）,and maternal dominance by environment interaction （DmE）.Linear functions of genetic components are listed for parent,F1,and F2.A set of parents,their reciprocal F1’s and F2’s is applicable for efficient analysis.Variance and covariance components can be well mated by MINQUE（O/l） with the jackknife procedure.The t-test conducted by the jackknife procedure is applicable for detecting significance of variation.Adjusted Unbiased Prediction （AUP） method is suggested for predicting genetic effects.

Cognitive and Behavioral Effects of Participatory Sex Education on the Dual Prevention of STI/HIV/AIDS and Unwanted Pregnancies among Adolescents in Kinshasa High Schools, DR Congo

Context: With the view to reorient both STI/HIV/AIDS prevention and adolescents pregnancies, this research study aims at evaluating cognitive and behavioral acquisitions, as well as the process of interactive sex education participatory approach among adolescents in Kinshasa high schools. Methods: Based on a “pre and post” virtually experimental design, two crosswise surveys were conducted in Kinshasa, for six months in 2011-2012 on 484 high school students (pre-survey) and on 441 high school students (post-survey), whose age range from 14 - 19 years including both sexes. Two participatory educational talks (PET) “A” and “B”, covered weekly in two different schools, were compared to a control group school. The PET “A” consisted of interactive interpersonal communication sessions given by an external expert as a substitute for the life education course in one school. The PET “B” carried out in another school, included more educational talk sessions, led by the external expert and supplemented by a close follow-up of teenagers divided into small groups of 10 participants. The subjects’ assessment was based on their knowledge, attitudes and practices relating to STI/HIV/AIDS dual prevention as well as to unwanted pregnancies. Results: The subjects involved in the PET “B” displayed a better/higher performance based on their knowledge, attitudes and practices related to STI/HIV/AIDS dual prevention and unwanted pregnancies. Broadly speaking, knowledge has been improved 6 times with the PET “B” (OR = 6, 10, IC 95%) (3.24 - 11.9), and 3 times with the PET “A” (OR = 3, 45, IC 95%) (1.79 - 6.81), compared to control school. Similarly, findings on subjects’ attitudes show an improvement rated 12 times with the PET”B” (OR = 11, 99, IC 95%) (5.67 - 27.38) and 5 times for the PET “A” (OR = 5.51, IC 95%) (2.54 - 12.87). As far as the subjects’ practices are concerned, an improvement of 6 more times of protected sexual intercourses with the PET “B” compared with the control school group (OR = 6, 52, IC 95%) (3.60 - 12.0). The process assessment records a spontaneous involvement of schools enhanced by the positive contribution of Life Education and Biology teachers;add a massive participation of adolescents who requested permanent PET program. Conclusion: The results of this study suggest that school sexual education programs can be improved to increase the quality of apprenticeship. The use of interactive methods and the consideration of the adolescents’ specific needs that take into account the gender approach may bring about beneficial advantages on both educational outcomes and reproductive health of adolescents.

Effects of Several Factors on Trapping Quantity of Grapholitha molesta Busck by Sex Pheromone

The key application technology for sex pheromone of Grapholitha molesta was studied from the aspects of different hanging heights and orientations, dif- ferent doses and types of traps through the tests on trapping quantity of G. molesta in fields. The results showed that the trapping effect was enhanced when the hanging height was increased, and the trapping effect was the best in west direction. The trapping effect was enhanced when the dosage was increased. When it was up to 6 lures, the trapping effect was the best with 38.75 head/trap; the next was 2 lures with 31.00 head/trap. All types of traps had trapping ability to G. mo- lesta, among which triangle trapper was the best, followed by self-made bottle trap. Their trapping effects were 138.75 and 100.25 head/trap, respectively.

多囊卵巢综合征不孕人群接受辅助生殖技术治疗后性激素水平的变化

目的探析不孕症人群中多囊卵巢综合征在接受辅助生殖技术治疗后的性激素水平变化,为治疗方案的选择提供依据。方法收集2016年1月至2021年6月期间于昆明医科大学第一附属医院就诊患者的病例资料,根据是否诊断为多囊卵巢综合征分为PCOS组(103名)和非PCOS组(589名),对比2组患者性激素变化情况。结果PCOS组的患者更为年轻,BMI更高,窦状卵泡数更多,AMH值更高,而Gn使用总量更低,PCOS组中LH/FSH>2的人数多于非PCOS组(P<0.05)。经过治疗,2组的LH值均有所减少,FSH、E_2和P都呈上升趋势(P<0.05);且PCOS组LH和E_2治疗前后的差值均大于非PCOS组(P<0.05)。结论相较于非PCOS不孕患者,PCOS不孕人群治疗前后的性激素指标变化更明显。为使多囊卵巢患者获得更好的临床疗效,建议在之后的治疗过程中关注相关性激素水平变化,合理选择治疗方案。

Control Effects of a New Sex Pheromone Trap and Biological Agents on Sesamia inferens Walker and Argyroploce schistaceana (Snellen)

This study was conducted to explore and seek high-efficiency and safe green control techniques on sugarcane borers,promote green control of diseases and pests of sugarcane and build a resource-conserving,environmentally friendly and sustainable governance system of diseases and pests of sugarcane.A new sex pheromone trap of sugarcane borers,abamectin· Bacillus thuringiensis (Bt) and tebufenozide were selected and applied in field trials.The use of the new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 0.05% abamectin·10 billion active gemmae/g B.thuringiensis (Bt) WP (1.8 kg/hm 2) or new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 200 g/L tebufenozide SC (1.5 L/hm 2) were the best in the sugarcane production.New sex pheromone trap of sugarcane borers should be installed in early march,and biological agents were mixed with water 900 kg per hectare and sprayed evenly sugarcane plants at the beginning of April.Their control effects of dead heart rate and bored stalk rate could be more than 69.98% and 49.09%,respectively,which were superior to that of the control pesticide 3.6% bisultap GR (90 kg/hm 2).The results showed that new sex pheromone trap of sugarcane borers in combination with abamectin· B.thuringiensis (Bt) or tebufenozide was the optimum mode of green control techniques on sugarcane borers.When it was alternately or coordinately used with other technology,it could delay the emergence and development of drug resistance,and it was worthy of widespread popularization and application in sugarcane area.

桃园主要鳞翅目害虫性诱芯的混用诱捕效果

【目的】明确北方桃园内梨小食心虫、苹小卷叶蛾、桃蛀螟和桃潜叶蛾等4种害虫性诱芯之间的互作效应。【方法】通过田间诱芯混合诱捕试验,比较4种害虫性诱芯之间的相互作用。【结果】2022年和2023年调查发现,单个梨小食心虫性诱芯与各梨小食心虫性诱芯组合在梨小食心虫诱捕数量上均无显著差异。单个桃潜叶蛾性诱芯与各桃潜叶蛾性诱芯组合在桃潜叶蛾诱捕数量上也均无显著差异。苹小卷叶蛾性诱芯+梨小食心虫性诱芯组合对苹小卷叶蛾的诱捕数量均显著低于各年单独使用苹小卷叶蛾性诱芯时的诱捕数量(p<0.05)。【结论】在诱捕梨小食心虫时,梨小食心虫性诱芯可以与桃潜叶蛾、苹小卷叶蛾、桃蛀螟等3种害虫性诱芯混用;在诱捕桃潜叶蛾时,桃潜叶蛾性诱芯可以与梨小食心虫、苹小卷叶蛾、桃蛀螟等3种害虫性诱芯混用;在诱捕苹小卷叶蛾时,苹小卷叶蛾性诱芯可以与桃潜叶蛾和桃蛀螟这两种害虫的性诱芯混用,不能与梨小食心虫性诱芯混用。

E8,E 10-12∶OH对梨小食心虫性诱剂的增效作用及其与信息素结合蛋白和普通气味结合蛋白的结合机制

【目的】(反,反)-8,10-十二碳二烯-1-醇[(8 E,10E)-dodecadien-1-ol,E8,E 10-12∶OH]是苹果蠹蛾Cydia pomonella的主要性信息素成分,对梨小食心虫Grapholita molesta性诱剂具有引诱增效作用。本研究旨在测定E8,E 10-12∶OH对梨小食心虫性诱剂的增效能力,确定主要结合E8,E 10-12∶OH的信息素结合蛋白(pheromone binding proteins,PBPs)和普通气味结合蛋白(general odorant binding proteins,GOBPs)。【方法】利用触角电位(electroantennogram,EAG)仪测定梨小食心虫雄成虫对0.002,0.02,0.2,2,20,200和2000μg E8,E 10-12∶OH激发的EAG反应;利用田间诱集试验测定E8,E 10-12∶OH对梨小食心虫性诱剂的增效能力;利用荧光竞争结合实验测定梨小食心虫信息素结合蛋白GmolPBP1,GmolPBP2和GmolPBP3及普通气味结合蛋白GmolGOBP1,GmolGOBP2和GmolGOBP3与E8,E 10-12∶OH的抑制常数K i;利用分子动力学模拟的方法预测GmolGOBP2结合E8,E 10-12∶OH的关键氨基酸;利用定点突变和荧光竞争结合实验验证GmolGOBP2结合E8,E 10-12∶OH的关键氨基酸残基及弱相互作用力。【结果】梨小食心虫雄成虫对E8,E 10-12∶OH表现出EAG反应,雄成虫对2000μg E8,E 10-12∶OH的EAG反应值最高[(0.57±0.14)mV]。在梨小食心虫性诱剂中添加200μg E8,E 10-12∶OH时,增效效果最佳,增效倍数达2.46倍。GmolGOBP2结合E8,E 10-12∶OH的能力最强[K i=(1.92±0.05)μmol/L],是结合E8,E 10-12∶OH的主要OBP。分子动力学模拟表明GmolGOBP2的Phe18,Ile100,Glu104,Val117和Phe124结合E8,E 10-12∶OH的自由能最低,分别为-1.18,-1.33,-3.34,-1.19和-1.58 kj/kg,预测是GmolGOBP2结合E8,E 10-12∶OH的重要位点,将以上5个残基定点突变为丙氨酸Ala后,突变体蛋白E104A和F124A失去了结合E8,E 10-12∶OH的能力,表明Glu104和Phe124是GmolGOBP2结合E8,E 10-12∶OH的关键氨基酸。【结论】E8,E 10-12∶OH是开发梨小食心虫高效性诱剂的理想增效剂,GmolGOBP2在梨小食心虫识别种间性信息素E8,E 10-12∶OH的过程中发挥着重要作用,Glu104和Phe124是GmolGOBP2结合E8,E 10-12∶OH的关键氨基酸残基。

生育性别偏好如何影响育龄女性的生育意愿?——基于社会经济地位的遮掩效应

在逐渐宽松的生育政策背景下,生育性别偏好会对育龄女性生育意愿产生影响。本文基于CFPS2018数据,采用泊松回归和Bootstrap方法,探讨了生育性别偏好、社会经济地位与育龄女性生育意愿三者之间的关系,并分析了生育性别偏好对不同群体女性生育意愿的空间异质性影响。研究发现:不同生育性别偏好对女性生育意愿的影响方向和强度不同。相较于无性别偏好,男孩偏好整体上对育龄女性生育意愿表现出显著的负向抑制作用,然而该抑制作用在东部和西部农村地区不显著;儿女双全偏好整体上表现出显著的正向促进作用,且在东部城镇地区促进作用更大;女孩偏好整体上对女性生育意愿影响不显著,然而在东部城镇地区表现出对生育意愿显著的抑制作用,而在西部农村地区发挥着显著正向促进作用。此外,社会经济地位在性别偏好和生育意愿之间发挥了遮掩效应,受教育水平的提升会弱化男孩偏好对生育意愿的抑制作用,收入水平的提高会削弱女孩偏好对生育意愿的促进作用。在当前生育率持续走低的严峻形势下,建议充分尊重女性生育自主权,并且实施区域差异化的生育支持措施以提高女性生育意愿。

腹腔镜联合亮丙瑞林治疗EMS伴不孕疗效及对血清性激素影响

目的:观察腹腔镜联合亮丙瑞林治疗子宫内膜异位症(EMS)伴不孕疗效及对血清雌二醇(E_(2))、卵泡刺激素(FSH)和抗缪勒管激素(AMH)的影响。方法:选取2019年4月-2022年4月本院接受治疗的EMS伴不孕患者103例,以接受腹腔镜治疗为对照组(52例),接受腹腔镜联合亮丙瑞林治疗为联合组(51例)。比较两组手术后治疗总有效率,手术前后血清FSH、E_(2)、AMH水平,血清肿瘤标志物糖链抗原125(CA125)、糖链抗原199(CA199)水平;两组并发症及术后1年妊娠率、复发率。结果:术后3个月,联合组总有效率(90.2%)高于对照组(73.1%);术后6个月联合组血清FSH、E_(2)水平均低于对照组(P<0.05),两组AMH无差异(P>0.05),联合组血清CA125(21.64±4.36 U/L)、CA199(24.36±2.24 U/L)水平均低于对照组(33.70±6.92 U/L、35.48±3.22 U/L)(P<0.05)。术后1年联合组妊娠率(86.3%)高于对照组(44.2%),复发率(7.8%)低于对照组(23.1%)(均P<0.05)。两组治疗期间并发症发生率(9.8%、5.8%)无差异(P>0.05)。结论:腹腔镜辅助亮丙瑞林治疗EMS伴不孕能够显著提高治疗效果,改善血清性激素及肿瘤标志物水平,提高妊娠率,减少复发,且安全性较好。

基于年龄-龄期两性生命表的甲氨基阿维菌素苯甲酸盐对白背飞虱的亚致死效应分析

【目的】甲氨基阿维菌素苯甲酸盐是高效治理鳞翅目(Lepidoptera)害虫的新型抗生素类生物源杀虫剂。本研究旨在探究甲氨基阿维菌素苯甲酸盐对水稻害虫白背飞虱Sogatella furcifera的亚致死效应。【方法】采用稻茎浸渍法以不同浓度甲氨基阿维菌素苯甲酸盐处理的水稻苗饲养白背飞虱3龄若虫,72 h后计算甲氨基阿维菌素苯甲酸盐对白背飞虱3龄若虫的LC_(10),LC_(25)和LC_(50)值;采用稻茎浸渍法以LC_(10),LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理的水稻苗饲养白背飞虱3龄若虫48 h,测定F0代雌成虫寿命和单雌产卵量;统计F_(1)代卵历期、1-5龄若虫历期、成虫寿命、成虫前历期、总发育历期、成虫产卵前期、成虫总产卵前期和单雌产卵量,构建年龄-龄期两性生命表;利用Timing-MSChart软件预测白背飞虱未来60 d种群动态。【结果】处理72 h时甲氨基阿维菌素苯甲酸盐对白背飞虱3龄若虫的LC_(50),LC_(25)和LC_(10)值分别为0.831,0.222和0.068 mg/L。与对照组相比,LC_(10),LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理使白背飞虱F0代雌成虫平均寿命分别缩短了7.19%,24.81%和34.21%,单雌产卵量分别减少22.24%,31.22%和41.53%;LC_(25)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代5龄雌若虫历期,LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代雄成虫寿命和总发育历期,LC_(25)和LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理显著延长了F_(1)代成虫产卵前期和总产卵前期,且显著降低了内禀增长率(r)和周限增长率(λ);LC_(50)浓度甲氨基阿维菌素苯甲酸盐处理下F_(1)代净增殖率(R_(0))显著降低,LC_(25)浓度甲氨基阿维菌素苯甲酸盐处理下F_(1)代平均世代周期(T)显著延长;LC_(10),LC_(25)和LC_(50)这3个亚致死浓度甲氨基阿维菌素苯甲酸盐处理明显抑制了白背飞虱种群增长。【结论】亚致死浓度甲氨基阿维菌素苯甲酸盐胁迫会显著影响白背飞虱成虫寿命和繁殖能力,并降低其后代种群数量,该研究结果可为农业田间科学防治白背飞虱提供理论依据。

米非司酮联合炔诺酮片治疗围绝经期功能性子宫出血的临床效果

目的 探讨米非司酮联合炔诺酮片治疗围绝经期功能性子宫出血的效果。方法 方便选取南通市通州区中医院于2021年1月—2023年6月收治的82例围绝经期功能性子宫出血患者为研究对象,按随机数表法分为两组。对照组(41例)予米非司酮治疗;观察组(41例)予米非司酮联合炔诺酮片治疗。比较两组临床效果、子宫内膜厚度、月经量、性激素水平与不良反应。结果 观察组治疗总有效率(97.56%)高于对照组(82.93%),差异有统计学意义(χ^(2)=4.987,P<0.05)。治疗后,两组患者子宫内膜厚度均变薄、月经量均减少、性激素水平降低,且观察组优于对照组,差异有统计学意义(P均<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论 米非司酮联合炔诺酮片治疗围绝经期功能性子宫出血,能缓解患者症状并调节性激素水平,且不良反应少。

复方制剂雌二醇片/雌二醇地屈孕酮片治疗妇女围绝经期综合征的临床效果研究

目的 研究对围绝经期综合征妇女应用复方制剂雌二醇片/雌二醇地屈孕酮片治疗的临床效果。方法 单纯随机选取2022年1—12月赣州市妇幼保健院收治的80例围绝经期综合征患者为研究对象。使用红蓝信封法分为对照组和研究组,每组40例。对照组患者行戊酸雌二醇片治疗,研究组患者行复方制剂雌二醇片/雌二醇地屈孕酮片治疗。比较两组患者临床效果。结果 治疗前,两组患者性激素水平变化对比,差异无统计学意义(P>0.05);治疗后,研究组患者黄体生成素(13.23±5.23)IU/L、卵泡生成素(23.63±7.44)IU/L均低于对照组,雌二醇水平(71.23±10.23)pmoL/L高于对照组,差异有统计学意义(P均<0.05)。研究组患者临床有效率(95.00%)高于对照组(80.00%),差异有统计学意义(χ^(2)=4.114,P=0.042)。研究组患者不良反应总发生率(2.50%)低于对照组(12.50%),差异有统计学意义(χ^(2)=3.913,P=0.047)。结论 对围绝经期综合征患者应用复方制剂雌二醇片/雌二醇地屈孕酮片治疗,可改善患者激素水平,提升治疗有效率,安全性高。

温阳促孕方辅助西药治疗多囊卵巢不孕症临床研究

目的:观察温阳促孕方辅助西药治疗多囊卵巢不孕症患者的临床效果。方法:回顾性分析100例多囊卵巢不孕症患者的临床资料,根据患者的临床治疗方案分为对照组和观察组各50例。对照组予以氯米芬治疗,观察组在对照组基础上予以温阳促孕方治疗。比较2组治疗前后性激素水平、卵巢体积、卵泡数目、子宫内膜厚度、卵巢动脉血流,并比较2组不良反应及妊娠情况。结果:治疗后,2组黄体生成素水平降低(P<0.05),且观察组低于对照组(P<0.05);2组雌二醇、卵泡刺激素水平均升高(P<0.05),且观察组高于对照组(P<0.05)。治疗后,2组卵巢体积减小(P<0.05),且观察组小于对照组(P<0.05);2组卵泡数目减少(P<0.05),且观察组少于对照组(P<0.05);2组子宫内膜厚度增加(P<0.05),且观察组厚于对照组(P<0.05)。治疗后,2卵巢动脉血流搏动指数(PI)及阻力指数(RI)均降低(P<0.05),且观察组低于对照组(P<0.05)。2组不良反应发生率比较,差异无统计学意义(P>0.05)。观察组临床妊娠率64.00%,高于对照组36.00%(P<0.05)。结论:温阳促孕方辅助西药治疗多囊卵巢不孕症,能改善性激素水平、子宫内膜厚度、卵巢动脉血流及妊娠情况,且具有一定安全性。

戈舍瑞林联合米非司酮治疗子宫肌瘤疗效及对性激素、TGF-β1、TSGF和子宫内膜影响

目的:观察戈舍瑞林联合米非司酮治疗子宫肌瘤疗效及对患者血清性激素、转化生长因子β1(TGF-β1)、肿瘤特异性生长因子(TSGF)和子宫内膜影响。方法:纳入2022年5月-2023年10月于本院收治的子宫肌瘤患者160例并随机分为对照组和观察组各80例,两组均给予米非司酮治疗,观察组联合给予戈舍瑞林治疗,均连续给药6个月。比较两组临床疗效,治疗前后性激素、TGF-β1、TSGF和子宫内膜厚度。结果:观察组总有效率(91.3%)高于对照组(80.0%),孕酮(3.12±1.05μmol/L)、睾酮(0.89±0.27μmol/L)、泌乳素(12.01±3.41μg/L)、TGF-β1(10.98±2.59 ng/L)、TSGF(27.86±7.84μmol/L)、子宫体积(176.42±94.51 cm^(3))、肌瘤体积(28.09±9.51 cm^(3))和子宫内膜厚度(0.87±0.05 cm)均低于对照组(4.67±1.87μmol/L、1.96±0.89μmol/L、15.42±3.98μg/L、12.43±2.76 ng/L、34.72±9.85μmol/L、209.88±98.75 cm^(3)、32.43±9.84 cm^(3)、0.94±0.09 cm)(均P<0.05);治疗不良反应观察组(18.8%)与对照组(15.0%)无差异(P>0.05)。结论:米非司酮治疗子宫肌瘤均有效,戈舍瑞林联合米非司酮治疗效果更佳,可更好地调节患者性激素,降低TGF-β1、TSGF水平,减小子宫体积、肌瘤体积和子宫内膜厚度,未增加不良反应。

培土固肾汤联合西药治疗黄体功能不全型先兆流产的保胎效果及对血清性激素、Th1/Th2细胞因子水平的影响

目的观察培土固肾汤联合西药治疗黄体功能不全型先兆流产的保胎效果及对血清性激素、Th1/Th2细胞因子水平的影响。方法选取该院2019年8月—2022年8月收治的86例黄体功能不全型先兆流产患者为研究对象,依照区组随机化分组法将其分为对照组和观察组各43例。对照组使用西药治疗,观察组则在对照组基础上联合培土固肾汤进行治疗。比较两组保胎效果,观察两组患者阴道止血时间和腹痛消失时间,对比治疗前后两组患者血清性激素[人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)、孕酮(progesterone,P)、雌二醇(estradiol,E2)]、Th1/Th2细胞因子[肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ-干扰素(γ-interferon,IFN-γ)、白细胞介素4(interleukin-4,IL-4)、白细胞介素10(interleukin-10,IL-10)]水平变化,统计治疗期间两组不良反应发生率。结果治疗2周后,观察组保胎效果明显高于对照组(P<0.05)。观察组阴道止血时间、腹痛消失时间均低于对照组(P<0.05)。治疗2周后,两组患者HCG、P、E2水平较治疗前均有升高,且观察组高于对照组(P<0.05);两组患者TNF-α、IFN-γ水平较治疗前均有降低,IL-4、IL-10水平较治疗前均有升高(P<0.05),且观察组TNF-α、IFN-γ水平低于对照组(P<0.05),IL-4、IL-10水平高于对照组(P<0.05)。治疗2周内,两组不良反应发生率比较差异无统计学意义(P>0.05)。结论培土固肾汤联合西药治疗黄体功能不全型先兆流产保胎效果显著,能有效改善患者血清性激素、Th1/Th2细胞因子水平,且药物安全性良好,值得临床应用推广。

聚焦超声消融术联合地诺孕素治疗子宫腺肌病疗效及对免疫功能影响

目的:探讨聚焦超声消融术(FUAS)联合地诺孕素对子宫腺肌病患者疼痛评分、贫血程度、血清指标、激素指标、免疫功能和安全性的影响。方法:将2022年11月-2023年11月本院诊治的子宫腺肌病86例,依据1:1比例随机分为对照组(n=43)和观察组(n=43),两组均采用FUAS治疗,观察组同时联合地诺孕素治疗,两组均治疗3个月。对比两组治疗前后疼痛评分、贫血程度、血清指标、激素指标、免疫功能,治疗不良反应。结果:治疗后观察组视觉模拟评分(1.47±1.03分)低于对照组(3.66±1.46分),血红蛋白(117.66±10.39g/L)、免疫球蛋白G(10.36±1.29g/L)、免疫球蛋白M(1.36±0.29g/L)水平高于对照组(107.52±9.22g/L、8.73±1.58g/L、0.89±0.21g/L),鼠双微基因2(1.44±0.39kU/L)、糖类抗原125(34.97±4.11IU/ml)、血管内皮生长因子(82.16±15.34pg/ml)、促卵泡激素(3.47±1.03U/L)、雌二醇(114.28±20.11pmol/L)、黄体生成素(4.27±1.05mg/ml)水平均低于对照组(2.36±0.47 kU/L、44.85±4.32IU/ml、98.46±17.28pg/ml、5.26±1.14U/L、134.69±21.20pmol/L、5.64±1.12mg/ml),不良反应发生率(9.3%)低于对照组(27.9%)(均P<0.05)。结论:FUAS联合地诺孕素治疗子宫腺肌病可有效减轻患者疼痛和贫血情况,改善血清因子指标、免疫功能和性激素指标,安全性较高。

不同激素治疗方案对女性围绝经期异常子宫出血的临床疗效分析

目的分析女性围绝经期异常子宫出血治疗中,不同激素治疗方案的实施价值。方法非随机选取2020年1月—2023年11月莘县妇幼保健院收治的88例围绝经期异常子宫出血患者为研究对象,按治疗方法不同分为两组,各44例。对照组给予单一炔诺酮片治疗、研究组在此基础上联用黄体酮胶囊治疗,比较两组患者子宫内膜厚度、血红蛋白水平、月经量、性激素指标水平及临床治疗有效率、不良反应发生情况。结果与对照组相比,研究组患者的血红蛋白水平更高,子宫内膜厚度更小、月经量更少,差异有统计学意义(P均<0.05);治疗后两组性激素指标优于治疗前,且治疗后研究组较对照组更低,差异有统计学意义(P均<0.05);研究组治疗有效率(97.73%)低于对照组(77.27%),差异有统计学意义(χ^(2)=4.838、8.416,P<0.05)。研究组不良反应发生率(2.27%)低于对照组(13.64%)。结论炔诺酮片+黄体酮胶囊联合治疗的实施效果更佳,可以帮助患者有效改善性激素指标状况,对降低子宫内膜厚度,提升血红蛋白水平具有积极意义,具有较高的应用价值。

经阴道CO_(2)激光联合盆底生物反馈电刺激治疗阴道松弛症的疗效分析

目的通过观察经阴道CO_(2)激光联合盆底生物反馈电刺激治疗阴道松弛症的疗效,并与传统的盆底功能康复治疗进行疗效比较,探讨该治疗方式是否能够有效改善患者的阴道松弛程度,且能进一步改善阴道松弛症患者的生活质量。方法选择2022年11月1日至2023年6月30日就诊于我院盆底康复门诊的阴道松弛症患者60例,随机分为试验组和对照组,每组30例。对照组进行盆底功能康复治疗,包括1个疗程(10次)的盆底生物反馈电刺激治疗,治疗间歇期进行凯格尔训练。试验组每月进行1次经阴道CO_(2)激光治疗,连续2次为1个疗程,治疗间歇期间进行1个疗程(10次)的盆底生物反馈电刺激治疗。分别于治疗前、治疗后1个月进行女性性功能指数量表、女性性不适量表及肌力测定,对两组患者进行疗效评定。结果试验组患者治疗后的快速收缩阶段肌力、紧张收缩阶段肌力、耐力收缩阶段肌力均高于对照组,差异具有统计学意义(P<0.05)。试验组患者治疗后的性欲望、主观性唤起能力、性生活满意度、性交痛评分以及FSFI总分均优于对照组,差异具有统计学意义(P<0.05);但治疗前后两组患者的性活动时阴道湿润性、性高潮评分比较无明显统计学意义(P>0.05)。试验组患者治疗后的FSDS-R评分相较于对照组明显改善,差异具有统计学意义(P<0.05)。结论阴道松弛症患者采用经阴道CO_(2)激光与盆底生物反馈电刺激联合的治疗方法,能显著改善患者盆底肌功能,促进盆底正常肌力的恢复,并且能有效地提升患者在性行为中的感受及满意程度,疗效确切,安全性高,在阴道松弛症的临床治疗中具有应用价值和推广前景。