目的观察电针对慢性应激抑郁大鼠行为学和肠道菌群每孔颜色平均变化率(Average well color development,AWCD)、Shannon-Wiener多样性指数的影响,探讨电针治疗抑郁症的作用机制。方法将60只SD大鼠随机分为正常组、模型组、调神畅情组和...目的观察电针对慢性应激抑郁大鼠行为学和肠道菌群每孔颜色平均变化率(Average well color development,AWCD)、Shannon-Wiener多样性指数的影响,探讨电针治疗抑郁症的作用机制。方法将60只SD大鼠随机分为正常组、模型组、调神畅情组和普通电针组,建立温和不可预见性应激抑郁模型,造模成功后予不同干预方法,观察各组大鼠行为学及肠道微生物AWCD、Shannon-Wiener多样性指数的变化。结果与正常组比较,模型组大鼠呈抑郁样表现,AWCD、Shannon-Wiener多样性指数均显著降低。与模型组比较,调神畅情组和普通电针组大鼠抑郁样症状明显改善,AWCD、Shannon-Wiener多样性指数均明显升高。结论电针能够改善慢性应激抑郁大鼠行为学表现,并提高其肠道微生物群落AWCD和Shannon-Wiener多样性指数,这可能是电针治疗抑郁症的作用机制之一。展开更多
From a dataset of macrobenthos obtained from 18 cruises from 2004 to 2013 in the Huanghe（Yellow River）Estuary and its adjacent areas,the composition and characteristics of macrobenthos were analyzed,and the applicab...From a dataset of macrobenthos obtained from 18 cruises from 2004 to 2013 in the Huanghe（Yellow River）Estuary and its adjacent areas,the composition and characteristics of macrobenthos were analyzed,and the applicability of the Shannon-Wiener Index（H′）,AZTI＇s Marine Biotic Index（AMBI） and multivariate AMBI（MAMBI） for assessing benthic habitat quality was compared.The results showed a total of 203 macrobenthos in the study area.The macrobenthos were dominated by polychaetes,followed by mollusks and crustaceans.The macrobenthic ecological groups were dominated by EGI,EGII and EGIII,which respectively accounted for 31.5%,36.0% and 21.2% of the total.There were significant differences between the evaluation results of the three indices.The ecological quality status（EQS） levels given by the AMBI were greater than those given by the H′ and M-AMBI.The AMBI could not reflect the differences between 11 sites but the H′ and M-AMBI could do.Moreover,the three indices responded well to the variations in salinity（S） and dissolved oxygen（DO） in the waters.The H′ and MAMBI also responded sensitively to the differences in physical parameters,such as water depth and sediment texture.The correlation between M-AMBI and environmental pressure gradient data was the strongest.The MAMBI could effectively distinguish degraded conditions from undegraded but the H′ and AMBI could not.Therefore,the M-AMBI reflected benthic habitat health well in the study areas.However,the objectivity of evaluation results of M-AMBI needs further verification by physical,chemical and biological methods.The thresholds also need further discussion.展开更多
文摘目的观察电针对慢性应激抑郁大鼠行为学和肠道菌群每孔颜色平均变化率(Average well color development,AWCD)、Shannon-Wiener多样性指数的影响,探讨电针治疗抑郁症的作用机制。方法将60只SD大鼠随机分为正常组、模型组、调神畅情组和普通电针组,建立温和不可预见性应激抑郁模型,造模成功后予不同干预方法,观察各组大鼠行为学及肠道微生物AWCD、Shannon-Wiener多样性指数的变化。结果与正常组比较,模型组大鼠呈抑郁样表现,AWCD、Shannon-Wiener多样性指数均显著降低。与模型组比较,调神畅情组和普通电针组大鼠抑郁样症状明显改善,AWCD、Shannon-Wiener多样性指数均明显升高。结论电针能够改善慢性应激抑郁大鼠行为学表现,并提高其肠道微生物群落AWCD和Shannon-Wiener多样性指数,这可能是电针治疗抑郁症的作用机制之一。
基金The National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers under contract No.U1406403the National Marine Public Welfare Research Project of China under contract No.201405007the Environmental Assessment Projects of State Oceanic Administration People's Republic of China under contract No.DOMEP(MEA)-01-01
文摘From a dataset of macrobenthos obtained from 18 cruises from 2004 to 2013 in the Huanghe（Yellow River）Estuary and its adjacent areas,the composition and characteristics of macrobenthos were analyzed,and the applicability of the Shannon-Wiener Index（H′）,AZTI＇s Marine Biotic Index（AMBI） and multivariate AMBI（MAMBI） for assessing benthic habitat quality was compared.The results showed a total of 203 macrobenthos in the study area.The macrobenthos were dominated by polychaetes,followed by mollusks and crustaceans.The macrobenthic ecological groups were dominated by EGI,EGII and EGIII,which respectively accounted for 31.5%,36.0% and 21.2% of the total.There were significant differences between the evaluation results of the three indices.The ecological quality status（EQS） levels given by the AMBI were greater than those given by the H′ and M-AMBI.The AMBI could not reflect the differences between 11 sites but the H′ and M-AMBI could do.Moreover,the three indices responded well to the variations in salinity（S） and dissolved oxygen（DO） in the waters.The H′ and MAMBI also responded sensitively to the differences in physical parameters,such as water depth and sediment texture.The correlation between M-AMBI and environmental pressure gradient data was the strongest.The MAMBI could effectively distinguish degraded conditions from undegraded but the H′ and AMBI could not.Therefore,the M-AMBI reflected benthic habitat health well in the study areas.However,the objectivity of evaluation results of M-AMBI needs further verification by physical,chemical and biological methods.The thresholds also need further discussion.
