Objective: To explore the therapeutic mechanism of Tiaoxin Recipe and Bushen Recipe (BSR, ) in treating Alzheimer’s d isease (AD). Methods: Sixty patients with AD were divided into 3 groups according to their MMSE an...Objective: To explore the therapeutic mechanism of Tiaoxin Recipe and Bushen Recipe (BSR, ) in treating Alzheimer’s d isease (AD). Methods: Sixty patients with AD were divided into 3 groups according to their MMSE and ADL scores before treatment, using randomized block design. They were treated separately with TXR, BSR and Donepezil (Dp) for 12 weeks. The changes of MMSE score, ADL score, neuro-psychology amount table score, including FOM, RVR, DS and BD, as well as the overall operational evaluation before and after treatment were analyzed. Results: The recognition and daily life capacity of patients in the 3 groups were improved after treatment. MMSE score in the TXR group increased from 16.10±1.94 scores before treatment to 17 90±2 59 scores after treatment, in the BSR group, from 16 15±2 16 to 17 50±2 59, and in the Dp group, from 17 35±1 90 to 19 60±3 39, all showed significant difference (all P<0 01). Change of ADL score showed that in the TXR group, it lowere d from 43 10±3 86 to 41 50±4 40, in the BSR group from 43 30±4 53 to 41 60±4 12 and in the Dp group, from 42 95±3 58 to 40 60±5 23, which also showed significan t difference (P<0 05 or P<0 01). Moreover, increase of FOM, RVR and DS score was shown in the TXR and the Dp group, and increase of RVR and BD score was shown in the BSR group, with significant difference in compar ison of corresponding score before and after treatment, inter-groups comparison showed significant difference of FOM score between the BSR and the Dp group ( P<0 01). Overall operational evaluation (total effective rate) in the TXR group was 70%, in the BSR group 65% and in Dp group 75%, with no significant difference among them. Conclusion: TXR and BSR are effective TCM drugs in treating AD展开更多
In the past, many Chinese artists have beenafraid they would be criticized for lagging behindthe world in their conceptions. They are eager todo something unconventional or unorthodox. Theiractions are very similar wi...In the past, many Chinese artists have beenafraid they would be criticized for lagging behindthe world in their conceptions. They are eager todo something unconventional or unorthodox. Theiractions are very similar with the trend Liu展开更多
Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Ch...Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Chinese medicine systems pharmacology(TCMSP)database with its analysis platform for HF as a disease model,the active ingredients of YXTF were filtered according to the oral bioavailability(OB)and druglikeness(DL),and the corresponding targets were predicted.The disease targets of HF were retrieved by Human Phenotype Ontology(HPO)database.Then,“YXTF compounds-Targets-HF”network was constructed in the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database,the network visualized through the Cytoscape software,and the key nodes of the network achieved according to Degree(D),Closeness Centrality(CC),and Betweenness Centrality(BC).Kyoto Encyclopedia of Genes and Genomes(KEGG)gene function enrichment analysis was carried out for the key nodes with the help of GATHER database platform.The rabbit model of HF was established by coarctation of the abdominal aorta and perfusion of propylthiouracil(PTU).The pathological changes and protein expression of transforming growth factor beta(TGF-β)protein were observed by western blotting.Results“YXTF compounds-Targets-HF”network consisted of 403 nodes,136 key nodes and 10 related KEGG pathways that included focal adhesions,cell cycles,mitogen-activated protein kinase(MAPK)signaling pathways,Toll-like receptor signaling pathways,Cytokine-cytokine receptor interactions,Gap junctions,adherens junctions,TGF-β signaling pathways,apoptosis and Wnt signaling pathways,respectively.The expression of TGF-β protein in myocardium decreased significantly.Conclusions According to the network pharmacology,the treatment of YXTF for HF might be related to 10 metabolic pathways.The effects of the multicompound-multitarget-multi pathways and the synergistic effect drawn from the compounds that act as the monarch,minister,adjuvant,guide medicines were revealed through this study.展开更多
Background Shen song Yang xin （SSYX） is a compound of Chinese medicine with the effect of increasing heart rate （HR）. This study aimed to evaluate its electrophysiological properties at heart and cellular levels. ...Background Shen song Yang xin （SSYX） is a compound of Chinese medicine with the effect of increasing heart rate （HR）. This study aimed to evaluate its electrophysiological properties at heart and cellular levels. Methods The Chinese miniature swines were randomly assigned to two groups, administered with SSYX or placebo for 4 weeks （n=8 per group）. Cardiac electrophysiological study （EPS） was performed before and after drug administration. The guinea pig ventricular myocytes were enzymatically isolated and whole cell voltage-clamp technique was used to evaluate the effect of SSYX on cardiac action potential （AP）. Results SSYX treatment accelerated the HR from （141.8±36.0） beats per minute to （163.0±38.0） beats per minute （P=0.013） without changing the other parameters in surface electrocardiogram. After blockage of the autonomic nervous system with metoprolol and atropin, SSYX had no effect on intrinsic HR （IHR）, but decreased corrected sinus node recovery time （CSNRT） and sinus atrium conducting time （SACT）. Intra cardiac EPS showed that SSYX significantly decreased the A-H and A-V intervals as well as shortened the atrial （A）, atrioventricular node （AVN） and ventricular （V） effective refractory period （ERP）. In isolated guinea pig ventricular myocytes, the most obvious effect of SSYX on action potential was a shortening of the action potential duration （APD） without change in shape of action potential. The shortening rates of APD30, APD50 and APD9o were 19.5%, 17.8% and 15.3%, respectively. The resting potential （Em） and the interval between the end of APD30 and APD90 did not significantly change. Conclusions The present study demonstrates that SSYX increases the HR and enhances the conducting capacity of the heart in the condition of the intact autonomic nervous system. SSYX homogenously decreases the ERP of the heart and shortens the APD of the myocytes, suggesting its antiarrhythmic effect without proarrhythmia.展开更多
Atrial fibrillation （AF） is the most common type of arrhythmia, which is reported to increase the incidence of stroke by five to seven tblds, leading to the irregular ventricular rhythm and adversely impacted life q...Atrial fibrillation （AF） is the most common type of arrhythmia, which is reported to increase the incidence of stroke by five to seven tblds, leading to the irregular ventricular rhythm and adversely impacted life quality, and the mortality could be increased as well by AF per se. Shen Song Yang Xin （SSYX） capsule is a novel traditional Chinese medicine composed of more than a dozen Chinese medicine herbs with significant antiarrhythmic effect. In the current study, the effect of SSYX capsule has been tested in an animal study and it was also indicated by randomized double-blinded, positive drug-controlled clinical trials, However, the working mechanism of SSYX capsule as antiarrhythmic drug is not completely clear yet. This study tried to investigate the effect of SSYX capsule on the myocardial electrophysiology of acute AF dog model.展开更多
Background Bu-Shen-Yi-Qi-Tang （BSYQT）,which is prescribed on the basis of clinical experience,is commonly used in clinics of traditional Chinese medicine （TCM） for asthma treatment.The components of BSYQT include ...Background Bu-Shen-Yi-Qi-Tang （BSYQT）,which is prescribed on the basis of clinical experience,is commonly used in clinics of traditional Chinese medicine （TCM） for asthma treatment.The components of BSYQT include Radix Astragali （RA）,Herba Epimedii （HE） and Radix Rehmanniae （RR）.The aim of this study was to compare the effect of granules and herbs of BSYQT on airway inflammation in asthmatic mice.Methods Sixty female BALB/c mice were randomly divided into the normal control （NC） group,asthmatic group （A）,decoction of granules of BSYQT treatment group （GD）,decoction of herbs of BSYQT treatment group （HD）,and dexamethasone treatment group （DEX）.The mouse asthmatic model was induced by ovalbumin （OVA） sensitization and challenge.GD and HD of BSYQT as well as DEX were prepared and administered by intragastric infusion.Airway hyperresponsiveness （AHR） to methacholine （Mch）,lung histopathology analysis,inflammatory mediators in serum （IL-4,IL-5,IL-17A,IFN-γ,and eotaxin） and in lung （IL-4,IL-5,IFN-γ,and eotaxin） were selected for investigation and comparison.Results Both GD and HD treatment could decrease airway resistance （RL） and increase dynamic compliance （Cdyn） to Mch compared with the A group （P ＜0.05）.HD treatment was more effective in RL reduction than Mch at doses of 3.125 and 6.25 mg/ml （P ＜0.05） and in Cdyn increase at Mch doses of 6.25 and 12.5 mg/ml （P ＜0.05）.There were no marked differences in RL reduction and Cdyn improvement between mice in HD and DEX groups （P ＞0.05）.Both GD and HD treatment markedly attenuated lung inflammation （P ＜0.05）,and HD treatment demonstrated more significant therapeutic function in alleviating lung inflammation than that of GD and DEX treatment （P ＜0.05）.Both GD and HD treatment resulted in a significant reduction in IL-4 and IL-17A levels and an increase in the IFN-γ level in serum compared with the A group （P ＜0.05）.The effect of HD in lowering the IL-4 and IL-17A level was significantly greater than that of GD （P ＜0.05）,and was not significantly different from DEX （P ＞0.05）.HD treatment significantly reduced the serum level of IL-5 and eotaxin compared with the A group （P ＜0.05）,however,mice in the GD treatment group did not demonstrate this effect.GD and HD treatment significantly reduced IL-4 and eotaxin mRNA expression compared with the A group （P ＜0.05）.HD treatment significantly reduced IL-5 mRNA expression compared with the A group （P ＜0.05）.There was a significant difference between the GD and HD treatment groups in reducing IL-5 and eotaxin mRNA expression （P ＜0.05）.HD treatment was more effective in down-regulation of IL-5 in serum and eotaxin level both in serum and lung than DEX （P ＜0.05）.Compared with the A group,an obvious increase in mRNA expression of IFN-γ was observed in both the GD and HD treatment groups （P ＜0.05）.However,the effect of HD treatment on increase of IFN-γ mRNA expression was more apparent than GD and DEX treatment （P ＜0.05）.Conclusions Both GD and HD treatment could decrease AHR,attenuate lung inflammation,reduce IL-4,IL-5,IL-17A,and eotaxin levels and increase IFN-γ levels in asthmatic mice.HD treatment manifests more remarkable inhibitory effects on asthmatic inflammation than GD treatment,which could provide a guide for further research on the screening of the material basis of the best anti-inflammatory effect of BSYQT.展开更多
文摘Objective: To explore the therapeutic mechanism of Tiaoxin Recipe and Bushen Recipe (BSR, ) in treating Alzheimer’s d isease (AD). Methods: Sixty patients with AD were divided into 3 groups according to their MMSE and ADL scores before treatment, using randomized block design. They were treated separately with TXR, BSR and Donepezil (Dp) for 12 weeks. The changes of MMSE score, ADL score, neuro-psychology amount table score, including FOM, RVR, DS and BD, as well as the overall operational evaluation before and after treatment were analyzed. Results: The recognition and daily life capacity of patients in the 3 groups were improved after treatment. MMSE score in the TXR group increased from 16.10±1.94 scores before treatment to 17 90±2 59 scores after treatment, in the BSR group, from 16 15±2 16 to 17 50±2 59, and in the Dp group, from 17 35±1 90 to 19 60±3 39, all showed significant difference (all P<0 01). Change of ADL score showed that in the TXR group, it lowere d from 43 10±3 86 to 41 50±4 40, in the BSR group from 43 30±4 53 to 41 60±4 12 and in the Dp group, from 42 95±3 58 to 40 60±5 23, which also showed significan t difference (P<0 05 or P<0 01). Moreover, increase of FOM, RVR and DS score was shown in the TXR and the Dp group, and increase of RVR and BD score was shown in the BSR group, with significant difference in compar ison of corresponding score before and after treatment, inter-groups comparison showed significant difference of FOM score between the BSR and the Dp group ( P<0 01). Overall operational evaluation (total effective rate) in the TXR group was 70%, in the BSR group 65% and in Dp group 75%, with no significant difference among them. Conclusion: TXR and BSR are effective TCM drugs in treating AD
文摘In the past, many Chinese artists have beenafraid they would be criticized for lagging behindthe world in their conceptions. They are eager todo something unconventional or unorthodox. Theiractions are very similar with the trend Liu
基金funding support from the National Natural Science Foundation of China (No. 81673955)the Education Department of Hunan Province (No. 16A158)+1 种基金Domestic First-class Construction Discipline Open Fund Project for Chinese Medicine of Hunan (No. 2018ZYX43)the Postgraduate Innovation Project of Hunan University of Chinese Medicine in 2018 (No. 2018CX43)
文摘Objective To explore the possible mechanism of Yi Xin Tai Formula(YXTF)in treating heart failure(HF)by network pharmacology method and to verify the related targets by western blotting.Methods Using the traditional Chinese medicine systems pharmacology(TCMSP)database with its analysis platform for HF as a disease model,the active ingredients of YXTF were filtered according to the oral bioavailability(OB)and druglikeness(DL),and the corresponding targets were predicted.The disease targets of HF were retrieved by Human Phenotype Ontology(HPO)database.Then,“YXTF compounds-Targets-HF”network was constructed in the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database,the network visualized through the Cytoscape software,and the key nodes of the network achieved according to Degree(D),Closeness Centrality(CC),and Betweenness Centrality(BC).Kyoto Encyclopedia of Genes and Genomes(KEGG)gene function enrichment analysis was carried out for the key nodes with the help of GATHER database platform.The rabbit model of HF was established by coarctation of the abdominal aorta and perfusion of propylthiouracil(PTU).The pathological changes and protein expression of transforming growth factor beta(TGF-β)protein were observed by western blotting.Results“YXTF compounds-Targets-HF”network consisted of 403 nodes,136 key nodes and 10 related KEGG pathways that included focal adhesions,cell cycles,mitogen-activated protein kinase(MAPK)signaling pathways,Toll-like receptor signaling pathways,Cytokine-cytokine receptor interactions,Gap junctions,adherens junctions,TGF-β signaling pathways,apoptosis and Wnt signaling pathways,respectively.The expression of TGF-β protein in myocardium decreased significantly.Conclusions According to the network pharmacology,the treatment of YXTF for HF might be related to 10 metabolic pathways.The effects of the multicompound-multitarget-multi pathways and the synergistic effect drawn from the compounds that act as the monarch,minister,adjuvant,guide medicines were revealed through this study.
文摘Background Shen song Yang xin （SSYX） is a compound of Chinese medicine with the effect of increasing heart rate （HR）. This study aimed to evaluate its electrophysiological properties at heart and cellular levels. Methods The Chinese miniature swines were randomly assigned to two groups, administered with SSYX or placebo for 4 weeks （n=8 per group）. Cardiac electrophysiological study （EPS） was performed before and after drug administration. The guinea pig ventricular myocytes were enzymatically isolated and whole cell voltage-clamp technique was used to evaluate the effect of SSYX on cardiac action potential （AP）. Results SSYX treatment accelerated the HR from （141.8±36.0） beats per minute to （163.0±38.0） beats per minute （P=0.013） without changing the other parameters in surface electrocardiogram. After blockage of the autonomic nervous system with metoprolol and atropin, SSYX had no effect on intrinsic HR （IHR）, but decreased corrected sinus node recovery time （CSNRT） and sinus atrium conducting time （SACT）. Intra cardiac EPS showed that SSYX significantly decreased the A-H and A-V intervals as well as shortened the atrial （A）, atrioventricular node （AVN） and ventricular （V） effective refractory period （ERP）. In isolated guinea pig ventricular myocytes, the most obvious effect of SSYX on action potential was a shortening of the action potential duration （APD） without change in shape of action potential. The shortening rates of APD30, APD50 and APD9o were 19.5%, 17.8% and 15.3%, respectively. The resting potential （Em） and the interval between the end of APD30 and APD90 did not significantly change. Conclusions The present study demonstrates that SSYX increases the HR and enhances the conducting capacity of the heart in the condition of the intact autonomic nervous system. SSYX homogenously decreases the ERP of the heart and shortens the APD of the myocytes, suggesting its antiarrhythmic effect without proarrhythmia.
文摘Atrial fibrillation （AF） is the most common type of arrhythmia, which is reported to increase the incidence of stroke by five to seven tblds, leading to the irregular ventricular rhythm and adversely impacted life quality, and the mortality could be increased as well by AF per se. Shen Song Yang Xin （SSYX） capsule is a novel traditional Chinese medicine composed of more than a dozen Chinese medicine herbs with significant antiarrhythmic effect. In the current study, the effect of SSYX capsule has been tested in an animal study and it was also indicated by randomized double-blinded, positive drug-controlled clinical trials, However, the working mechanism of SSYX capsule as antiarrhythmic drug is not completely clear yet. This study tried to investigate the effect of SSYX capsule on the myocardial electrophysiology of acute AF dog model.
文摘Background Bu-Shen-Yi-Qi-Tang （BSYQT）,which is prescribed on the basis of clinical experience,is commonly used in clinics of traditional Chinese medicine （TCM） for asthma treatment.The components of BSYQT include Radix Astragali （RA）,Herba Epimedii （HE） and Radix Rehmanniae （RR）.The aim of this study was to compare the effect of granules and herbs of BSYQT on airway inflammation in asthmatic mice.Methods Sixty female BALB/c mice were randomly divided into the normal control （NC） group,asthmatic group （A）,decoction of granules of BSYQT treatment group （GD）,decoction of herbs of BSYQT treatment group （HD）,and dexamethasone treatment group （DEX）.The mouse asthmatic model was induced by ovalbumin （OVA） sensitization and challenge.GD and HD of BSYQT as well as DEX were prepared and administered by intragastric infusion.Airway hyperresponsiveness （AHR） to methacholine （Mch）,lung histopathology analysis,inflammatory mediators in serum （IL-4,IL-5,IL-17A,IFN-γ,and eotaxin） and in lung （IL-4,IL-5,IFN-γ,and eotaxin） were selected for investigation and comparison.Results Both GD and HD treatment could decrease airway resistance （RL） and increase dynamic compliance （Cdyn） to Mch compared with the A group （P ＜0.05）.HD treatment was more effective in RL reduction than Mch at doses of 3.125 and 6.25 mg/ml （P ＜0.05） and in Cdyn increase at Mch doses of 6.25 and 12.5 mg/ml （P ＜0.05）.There were no marked differences in RL reduction and Cdyn improvement between mice in HD and DEX groups （P ＞0.05）.Both GD and HD treatment markedly attenuated lung inflammation （P ＜0.05）,and HD treatment demonstrated more significant therapeutic function in alleviating lung inflammation than that of GD and DEX treatment （P ＜0.05）.Both GD and HD treatment resulted in a significant reduction in IL-4 and IL-17A levels and an increase in the IFN-γ level in serum compared with the A group （P ＜0.05）.The effect of HD in lowering the IL-4 and IL-17A level was significantly greater than that of GD （P ＜0.05）,and was not significantly different from DEX （P ＞0.05）.HD treatment significantly reduced the serum level of IL-5 and eotaxin compared with the A group （P ＜0.05）,however,mice in the GD treatment group did not demonstrate this effect.GD and HD treatment significantly reduced IL-4 and eotaxin mRNA expression compared with the A group （P ＜0.05）.HD treatment significantly reduced IL-5 mRNA expression compared with the A group （P ＜0.05）.There was a significant difference between the GD and HD treatment groups in reducing IL-5 and eotaxin mRNA expression （P ＜0.05）.HD treatment was more effective in down-regulation of IL-5 in serum and eotaxin level both in serum and lung than DEX （P ＜0.05）.Compared with the A group,an obvious increase in mRNA expression of IFN-γ was observed in both the GD and HD treatment groups （P ＜0.05）.However,the effect of HD treatment on increase of IFN-γ mRNA expression was more apparent than GD and DEX treatment （P ＜0.05）.Conclusions Both GD and HD treatment could decrease AHR,attenuate lung inflammation,reduce IL-4,IL-5,IL-17A,and eotaxin levels and increase IFN-γ levels in asthmatic mice.HD treatment manifests more remarkable inhibitory effects on asthmatic inflammation than GD treatment,which could provide a guide for further research on the screening of the material basis of the best anti-inflammatory effect of BSYQT.
