PROTECTIVE EFFECT OF MOXIBUSTION AT SHENQUE(RN 8)ON GASTRIC MUCOSA

Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.

艾灸神阙穴对原发性痛经模型大鼠的作用及其机理

目的:探究艾灸神阙穴对原发性痛经模型大鼠的作用及其机制。方法:通过阴道涂片实验确定大鼠动情周期后使用苯甲酸雌二醇联合缩宫素制备原发性痛经大鼠模型。通过观测原发性痛经大鼠体质量、脏器指数、扭体潜伏期和扭体分数变化,检测各组大鼠血清性激素、前列腺素和疼痛因子等各项指标水平,比较艾灸治疗原发性痛经大鼠药效差异,其艾灸种类包括有烟艾灸与无烟艾灸。结果:在大鼠一般情况和行为学测试观察中,模型组比空白组扭体反应显著增加(P<0.01),表明造模成功;而与模型组比较,两个艾灸组大鼠体质量减轻相对缓慢,扭体潜伏期显著增加,扭体反应显著减少(P<0.05),其中有烟艾灸组和无烟艾灸组、阳性药物组大鼠扭体抑制率分别为54.89%、37.21%、68.82%。与模型组比较,两个艾灸组大鼠血清中雌二醇、缩宫素、前列腺素F_(2α)、白介素-6浓度均显著降低(P<0.05),前列腺素E_(2)和β-内啡肽水平显著升高(P<0.05)。结论:艾灸大鼠神阙穴能够有效缓解原发性痛经,调节机体性激素、前列腺素和疼痛相关因子可能是其潜在的作用机制。

中药外敷神阙穴联合电磁波治疗肝硬变腹水80例

目的:观察中药外敷神阙穴联合电磁波治疗肝硬变腹水的临床疗效。方法:选取2013年1月—2014年1月在本院住院的肝硬变腹水患者80例,随机分为治疗组和对照组,每组40例。对照组患者给予常规对症治疗,治疗组在对照组治疗的基础上给予中药外敷联合电磁波治疗。结果:对照组有效率67.5%,治疗组有效率95.0%,治疗组优于对照组(P<0.05);两组患者治疗前后肝功能比较,有显著性差异(P<0.05),两组之间比较无显著性差异(P>0.05);治疗组治疗后Child-Pugh评分优于对照组(P<0.05)。结论:中药外敷神阙穴联合电磁波治疗肝硬变腹水临床疗效确切。

Clinical Study on Moxibustion at Shenque(CV 8)for Chronic Urticaria

Objective:To observe the safety and efficacy of moxibustion at Shenque(CV 8)for chronic urticaria.Methods:A total of 80 cases who met the inclusion criteria were randomly allocated into a treatment group and a control group,40 in each.Cases in the treatment group were treated with thunder-fire moxibustion at Shenque(CV 8),whereas cases in the control group were treated with Mizolastine.Changes in clinical symptoms such as itching and skin lesion were observed before and after treatment and the therapeutic efficacies were assessed.Results:After treatment,the total scores of clinical symptoms in both groups were markedly reduced(P<0.05),and the reduction was more significant in the treatment group than that in the control group(P<0.05).The total effective rate in the treatment group was 77.5%,versus 65.0%in the control group,showing no significant difference(P>0.05).The Ridit analysis showed a between-group significant difference in therapeutic efficacy grades(P<0.05).There were no reports of adverse reactions in the treatment group and 2 cases experienced mild somnolence in the control group.Conclusion:With an exact effect for chronic urticaria,moxibustion at Shenque(CV 8)obtained better results in overall efficacy and improving symptoms than oral administration of Mizolastine.

Effect of moxibustion at Shenque （CV 8） on free radical metabolism in rat＇s hippocampus after different degrees of exhaustive exercise

Objective： To investigate the effect of moxibustion at Shenque （CV 8） on free radical metabolism in rat＇s hippocampus after different degrees of exhaustive exercise. Methods： A total of 72 male Sprague-Dawley （SD） rats were randomly divided into a normal group （n=8）, a model group （n=32） and a moxibustion group （n=32）. According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup （n=8）; the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup （n=8）. Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque （CV 8） for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde （MDA）, as well as the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in rat＇s hippocampus in each group were detected 24 h after the exhaustive exercise. Results： Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup （P〈0.01）, while it was significantly shortened in the 7-time and 10-time model subgroups （both P〈0.01）. Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged （both P〈0.01）. Compared with the normal group, the MDA concentration was increased significantly （P〈0.01）, and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup （both P〈0.05）; the MDA concentration was increased （all P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were decreased differently （P〈0.05 or P〈0.01） in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced （P〈0.05 or P〈0.01）, and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups （all P〈0.01）. Conclusion： Moxibustion at Shenque （CV 8） can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat＇s hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque （CV 8） against exercise-induced fatigue.

Experimental study on the effect of moxibustion at Shenque(CV 8) for long-term exercise-induced fatigue

Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a model group and a moxibustion group,10 rats in each group.Except rats in the blank group,the remaining rats were subjected to create long-term exhaustion models by repeated swimming.After successful modeling,rats in the moxibustion group received mild moxibustion at Shenque(CV 8)for 15 min,once every other day with a total of 10 times.Rats in the model group and the blank group did not receive moxibustion.At the end of the treatment,the exhausted times,and the body weight of rats before and after the experiment were compared among groups.The levels of blood malondialdehyde(MDA)and urea nitrogen(BUN),as well as the activities of aspartate transarninase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were also measured by the automatic biochemical analyzer,24 h after the exhausting excise.Results:The 10 th swimming time was significantly longer in the moxibustion group than that in the model group(P〈0.01).The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7 th and the 10 th exhausting excise(P〈0.05,P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the model group were higher than those in the blank group(all P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the moxibustion group were lower than those in the model group(P〈0.01).Conclusion:Moxibustion at Shenque(CV 8)can decrease the serum levels of MDA and BUN,as well as activities of AST,ALT and LDH in the long-term fatigue rats,thus to improve the symptoms of fatigue.

清胃活血汤加减联合中药贴敷神阙穴治疗急性胃炎临床研究

目的:观察清胃活血汤加减联合中药贴敷神阙穴治疗急性胃炎的临床疗效。方法:将98例急性胃炎患者按照随机数字表法分为对照组和治疗组,每组各49组。对照组给予泮托拉唑钠肠溶片治疗,治疗组在对照组治疗的基础上给予清胃活血汤加减联合中药贴敷神阙穴治疗。比较两组患者的临床疗效、临床症状缓解时间及治疗前后中医证候积分、胃肠激素指标[胃促生长素(Ghrelin)、P物质(substance P,SP)、胃泌素(gastrin, GAS)、5-羟色胺(5-hydroxytry ptamine, 5-HT)、胃动素(motilin, MOT)]以及胃镜检查结果变化情况。结果:对照组有效率为71.43%,治疗组有效率为87.75%,两组有效率比较,差异具有统计学意义(P<0.05)。两组患者治疗后中医证候积分低于本组治疗前,且治疗后治疗组低于对照组,差异具有统计学意义(P<0.05)。治疗组腹泻、腹痛、呕吐以及发热缓解时间均短于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后Ghrelin、MOT高于本组治疗前,SP、GAS、5-HT低于本组治疗前,且组间比较,差异均有统计学意义(P<0.05)。两组患者治疗后Ghrelin、MOT高于本组治疗前,SP、GAS、5-HT低于本组治疗前,且组间比较,差异均有统计学意义(P<0.05)。两组患者治疗后黏膜充血、水肿、糜烂以及出血发生率低于本组治疗前,且治疗后治疗组低于对照组,差异均有统计学意义(P<0.05)。结论:清胃活血汤加减联合中药贴敷神阙穴治疗急性胃炎,可明显改善患者的临床症状及胃肠激素水平,且对胃黏膜具有一定的保护作用。

Effect of moxibustion at Shenque (CV8) on immune system in rats with different degrees of exhaustive exercise

Objective: To investigate the effect of moxibustion at Shenque (CV8) on the immune system in rats with different levels of exhaustive exercise. Methods: Fifty-six male Sprague-Dawley (SD) rats were randomly divided into a blank group (n=8), an exhaustive group (n=24), and a moxibustion group (n=24). The exhaustive group was randomly divided into a 1-time exhaustive group, a 4-time exhaustive group and a 7-time exhaustive group, with 8 rats in each group. According to the treatment time, the moxibustion group was randomly divided into a 1-time moxibustion group, a 4-time moxibustion group and a 7-time moxibustion group, with 8 rats in each group. Rats in the exhaustive groups and the moxibustion groups were subjected to replicating the exhaustive swimming models. Rats in each moxibustion group received mild moxibustion for 15 min immediately after the exhaustive modeling, once every other day. Twenty-four hours after the corresponding exhaustive exercise, the rats in each group were tested for the levels of serum immunoglobulin (Ig) G, IgA, IgM and acid phosphatase (ACP), and the morphological changes of spleen tissues were observed. The level of IgA was detected by immunoturbidimetric assay, and the levels of IgG, IgM and ACP were detected by en zyme-linked imm uno sorb ent assay (ELISA). Results: Compared with the 1-time exhaustive group, swimming time of rats in the 4-time exhaustive group was significantly proIonged (P<0.01), and swimming time of rats in the 7-time exhaustive group was significantly shortened (P<0.01). Compared with the 7-time exhaustive group, exhaustive swimming time of rats in the 7-time moxibustion group was significantly proIonged (P<0.01). Compared with the blank group, the IgG level in the 1-time exhaustive group was significantly decreased (PvO.Ol), and the levels of IgG, IgA and IgM in the 4-time exhaustive group and the 7-time exhaustive group were all sign ifica ntly decreased (P<0.05 or PvO.Ol), while the ACP level was in creased sign ifica ntly (both P<0.01). Microscopically, the number of splenic corpuscles in the 1-time exhaustive group was reduced;the center of some splenic corpuscles in the 4-time exhaustive group was damaged;the number of splenic corpuscles in the 7-time exhaustive group was reduced, and there was no obvious germinal center. Compared with the 4-time exhaustive group, the IgA level in the 4-time moxibusti on group was sign ifica ntly in creased (P<0.01), and the ACP level was sign ifica ntly decreased (P<0.01). Compared with the 7-time exhaustive group, the levels of IgG, IgA and IgM in the 7-time moxibustion group were sign ifica ntly in creased (all PvO.Ol), and the ACP level was sign ifica ntly decreased (P<0.01). Microscopically, the nu mber of splenic corpuscles in the 1-time moxibustion group was reduced;the center of some splenic corpuscles in the 4-time moxibustion group was damaged together with hyperplasia of some splenic corpuscles;blast cells were proliferated in the center of some splenic corpuscles in the 7-time moxibustion group. Conclusion: Moxibustion at Shenque (CV 8) can improve the levels of IgG, IgA and IgM, reduce the ACP level, repair damaged spleen tissues, and enhance the immunity of the body to some extent in the Iong-term fatigue rats.

Identification and characterization of murine adipose tissue-derived somatic stem cells of Shenque(CV8)acupoint

Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2.6000±0.5477 vs.0.8000±0.8367,t=4.025,P=0.0038)were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint.However,cells possessed significantly weaker osteogenicity([2.697±0.627]%vs.[7.254±0.958]%,t=6.893,P=0.0023)in the CV8 acupoint group.Conclusions:Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells.The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.

Acupoint Sticking at Shenque(CV 8) with Ginger-prepared Ban Xia(Rhizoma Pinelliae) for Nausea and Vomiting Induced by Amifostine

Objective： To observe the treatment effect of acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） on nausea and vomiting induced by Amifostine for myelodysplastic syndromes （MDS）. Methods： Totally 124 eligible subjects intervened by Amifostine were randomized into 2 groups by the visiting order,an observation group and a control group,62 in each group. The control group was intervened by conventional treatment, while the observation group was by acupoint sticking at Shenque （CV 8） with ginger-preparedBan Xia （Rhizoma Pinelliae） in addition to the same conventional treatment. The occurrence rate of nausea and vomiting in the two groups were observed. Results： After intervention, the occurrence rate of nausea and vomiting in the observation group was significantly lower than that in the control group （P＆lt;0.01）. Conclusion： Acupoint sticking at Shenque （CV 8） with ginger-prepared Ban Xia （Rhizoma Pinelliae）can produce a content effect on nausea and vomiting induced by Amifostine for MDS.

Clinical observation of Da Huang (Rheum Officinale) application at Shenque(CV 8) for constipation after operation for lumbar vertebral fracture

Objective： To observe the effect of Da Huang（Rheum Officinale） application at Shenque（CV 8） on constipation after operation for lumbar vertebral fracture.Methods： Seventy-four patients with constipation after operation for lumbar vertebral fracture were divided into an observation group and a control group by the random number table, 37 cases in each group. The two groups both received ordinary treatment（including healthcare guidance, emotional care, diet arrangement, and defecation nursing）, while the observation group was additionally given Da Huang（Rheum Officinale） application at Shenque（CV 8） for 6 h each day. The therapeutic efficacy was evaluated after successive 3-day treatment. Results： After 3-day treatment, it took significantly less time for the observation group to conduct the first flatulence and defecation than for the control group（P〈0.05）; the therapeutic efficacy of the observation group was significantly higher than that of the control group（P〈0.01）. Conclusion： Da Huang（Rheum Officinale） application at Shenque（CV 8） is effective in treating constipation after lumbar vertebral fracture operation, and it＇s easy-to-operate and well accepted by patients, hence it＇s proper to promote this method in clinic.

Effect of moxibustion at Shenque(CV 8)on myocardial remodeling and function in exercise-induced fatigue rats

Objective To explore the effect of moxibustion at Shenque(CV 8)on myocardial structure and function in exercise-induced fatigue rats.Methods A 12-week treadmill running training was performed to create an exercise-induced fatigue rat model.Sixty eligible male specific-pathogen-free grade Sprague-Dawley rats were randomly divided into a blank group,a control group,a model group,a non-meridian non-acupoint group,a Zusanli(ST 36)group and a Shenque(CV 8)group,with 10 rats in each group.Rats in the blank group did not receive treadmill running training or moxibustion.Rats in the control group did not receive treadmill running training but received mild moxibustion at Shenque(CV 8).Rats in the model group received treadmill running training but no moxibustion.Rats in the non-meridian non-acupoint group,the Zusanli(ST 36)group and the Shenque(CV 8)group received moxibustion at the non-meridian non-acupoint points,Zusanli(ST 36)or Shenque(CV 8)immediately after each treadmill running training,15 min each time,once a day for 5 consecutive days a week at a 2-day interval,60 times of moxibustion in total.Left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVESd),left ventricular diastolic volume(LVDv),left ventricular systolic volume(LVSv),ejection fraction(EF),stroke volume(SV),early diastolic peak flow velocity of mitral valve(E)and late diastolic peak flow velocity of mitral valve(A)of each group before and after the last treadmill running training were measured.Blood was collected 6 h after the last treadmill running training,and serum C-reactive protein(CRP),myoglobin(Mb),creatine kinase-myocardial band(CK-MB),cardiac troponin I(cTnI)and cardiac troponin T(cTnT)levels were detected.Finally,the heart was separated,the heart mass(HM)was measured,the cTnT level of the myocardial tissue was detected,the ultrastructural changes of the left ventricular myocardium were observed by transmission electron microscope,the left ventricular fraction shortening(LVFS),E/A and heart mass index(HMI)were calculated.Results Compared with the same group before treatment,the rat cardiac LVEDd,LVESd,LVDv,LVSv,SV,E and A were significantly increased(all P<0.01),and the rat LVFS,E/A and EF were significantly decreased(all P<0.01)in the model group and the non-meridian non-acupoint group after treatment;the rat cardiac SV,LVDv,LVSv,E and A were all increased(all P<0.01),while E/A and EF were decreased(all P<0.01)in the Zusanli(ST 36)group after treatment;the rat cardiac LVDv,E and A were significantly increased(P<0.01 or P<0.05),and E/A was significantly decreased(P<0.01)in the Shenque(CV 8)group after treatment.After treatment,compared with the blank group,the rat cardiac LVEDd,LVESd,SV,LVDv,LVSv,E,A,the serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and the myocardial cTnT were increased(all P<0.01),and the LVFS,E/A and EF were all reduced(all P<0.01)in the model group;compared with the model group and the non-meridian non-acupoint group,rats in the Zusanli(ST 36)group and the Shenque(CV 8)group showed decreased LVEDd,LVESd,SV,LVDv,LVSv,E,A,serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and myocardial cTnT(P<0.01 or P<0.05),along with increased LVFS,E/A and EF(all P<0.01);compared with the Zusanli(ST 36)group,Mb and A of the Shenque(CV 8)group were decreased(both P<0.01),while both E/A and EF were increased(P<0.01,P<0.05).Transmission electron microscopy examination showed that myofibrils in the blank group and the control group were neatly arranged with clear light and dark bands;the model group and the non-meridian non-acupoint group showed different degrees of myofibril disintegration and breakage,increased and aggregated mitochondria of different sizes,and increased electron density.The myofibrils in the Shenque(CV 8)group and Zusanli(ST 36)group were arranged neatly with clear light and dark bands,and compensatory hyperplasia of mitochondria.Conclusion Moxibustion at Shenque(CV 8)and Zusanli(ST 36)both can effectively improve the occurrence of myocardial remodeling in exercise-induced fatigue rats,and the effect of moxibustion at Shenque(CV 8)is better in improving cardiac function.

Observation of therapeutic effects of point application at Shenque(CV 8)plus moxa-salt hot compress for prevention of gastrointestinal adverse reactions after chemotherapy for non-Hodgkin Iymphoma

Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.

Effect of acupoint sticking at Shenque （CV 8） for preventing spleen-stomach disharmony caused by venous analgesia pump

Objective： To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque （CV 8） for preventing spleenstomach disharmony caused by venous analgesia pump. Methods： A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table. There were 58 patients in the control group who received conventional post-surgery nursing care, and there were 62 patients in the treatment group who received acupoint sticking at Shenque （CV 8） on the basis of conventional nursing care. After treatment, the incidences of postoperative nausea and vomiting, and abdominal bloating were measured. Results： The total incidence of nausea, vomiting and abdominal bloating in the treatment group was 11.3%, versus 39.7% in the control group, and the difference of the incidence between the two groups showed a statistical significance （P〈0.05）. In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery, cases in the treatment group showed lower severity than those in the control group （P〈0.05）. Conclusion： Acupoint sticking at Shenque （CV 8） can effectively prevent spleen-stomach disharmony caused by venous analgesia pump, which will alleviate sufferings of the patients.

中药贴敷神阙治疗阿片类药物相关性便秘疗效的系统评价

目的:本研究系统评价了中药贴敷神阙治疗癌症患者阿片类药物相关性便秘的疗效和安全性,以期为临床治疗提供一定参考。方法:计算机检索PubMed、EMBase、Cochrane Library、CNKI、维普网和万方数据库,收集中药贴敷神阙治疗癌症患者阿片类药物相关性便秘的RCT,按照标准提取资料,根据Cochrane手册5.1.0评价文献质量后采用RevMan 5.3软件进行Meta分析。结果:纳入22篇文献,包含1 777例患者。Meta分析示,中药贴敷神阙治疗癌症患者阿片类药物相关性便秘疗效明显,差异有统计学意义(RR=1.15,95%CI[1.06,1.26])。亚组分析示:贴敷组较护理组、贴敷联合其他外治组较标准观察组有效率提高{RR=1.35,95%CI[1.24,1.48];RR=1.34,95%CI[1.20,1.48]}。贴敷+酚酞片VS酚酞片、贴敷+乳果糖VS乳果糖差异无统计学意义,但有提高疗效的可能性{RR=1.20,95%CI[1.00,1.43];RR=1.19,95%CI[0.94,1.49]},7篇文献报道了不良反应,示不良反应较轻,治疗安全。结论:中药贴敷神阙能有效的治疗恶性肿瘤患者阿片类药物相关性便秘,并能明显提高西药治疗的有效率。受纳入文献质量的限制,本研究尚不能得到较为可靠的结论。

隔物灸对寒湿凝滞型原发性痛经患者经期血浆β-EP含量的影响

目的:探讨隔物灸治疗寒湿凝滞型原发性痛经的作用机制。方法:以隔物灸治疗寒湿凝滞型原发性痛经患者105例(隔物灸组),并设中药月月舒作对照104例(药物组),观察临床疗效,同时对两组各40例进行了治疗前后经期β-内啡肽(β-EP)含量的变化检测。结果:隔物灸组总有效率为95.2%,优于药物组的85.6%(P<0.05);隔物灸组β-EP水平较治疗前明显升高(P<0.01)。结论:隔物灸对寒湿凝滞型原发性痛经患者有明显的治疗作用,其作用机制之一可能是通过调节血浆中β-EP的水平而发挥止痛效应。

神阙隔药灸疗法治疗溃疡性结肠炎的临床观察

目的观察神阙隔药灸疗法治疗溃疡性结肠炎(UC)的疗效及对自身免疫水平的影响,并探讨其作用机制。方法将60例UC患者随机分为治疗组(30例)和对照组(30例)。治疗组采用神阙隔药灸疗法,对照组口服柳氮磺胺吡啶片和甲硝唑片,比较两组治疗前后主要症状及肠黏膜病变变化和总疗效,观察血清免疫球蛋白、外周血T细胞亚群与自然杀伤细胞(NK)含量变化。结果治疗组显效率、总有效率分别为60·0%、86·7%,明显优于对照组的23·3%、66·7%,两组间比较差异有显著性或非常显著性意义(P<0·05,P<0·01)。治疗组治疗后IgG显著降低,与对照组治疗后比较差异有显著性意义(P<0·05);治疗前后IgA、IgM无明显变化(P>0·05)。治疗组治疗后外周血T细胞亚群与NK细胞含量均不同程度增高,增高幅度与疗效呈正相关。结论神阙隔药灸疗法是治疗溃疡性结肠炎的满意方法,其作用机制可能通过调节机体的免疫功能发挥疗效。

隔药灸脐法治疗原发性痛经临床研究

目的观察隔药灸脐与针刺三阴交穴对原发性痛经患者症状的影响,比较其疗效差异。方法将60例原发性痛经患者随机分为灸脐组和针刺组,每组30例。观察治疗3个月经周期后COX痛经症状评分量表(the Cox Menstrual Symptom Scale)的变化,并比较两组之间的差异。结果灸脐组与针刺组治疗前后COX痛经症状评分比较差异均有统计学意义(P<0.05),且灸脐组疗效优于针刺组(P<0.05)。结论隔药灸脐法治疗原发性痛经疗效较好。

隔药灸脐法治疗脾虚型肠易激综合征临床研究

目的比较隔药灸脐法与隔淀粉灸脐法治疗脾虚型肠易激综合征的疗效差异。方法选取40例脾虚泄泻型肠易激综合征患者,随机分成两组,每组20例,观察组采用隔药灸,对照组采用隔淀粉灸,对两组疗效进行比较。结果两组脾虚泄泻症状评分在治疗期和随访期与治疗前比较均有统计学差异(P<0.01),且隔药灸脐较隔淀粉灸脐可以更好地改善患者泄泻症状。结论脐疗可以有效地改善泄泻症状,隔药灸脐优于隔淀粉灸脐。