BACKGROUND: Ceramide galactosyltransferase (CGT) protein and mRNA expression defect can cause the abnormal morphology and slowing conduction velocity of peripheral nerve. Morphologic change and functional disorder ...BACKGROUND: Ceramide galactosyltransferase (CGT) protein and mRNA expression defect can cause the abnormal morphology and slowing conduction velocity of peripheral nerve. Morphologic change and functional disorder of myelin sheath and axon appear when diabetic peripheral neuropathy (DPN) occurs. Whether electroacupuncture at Zusanfi(ST 36) and Shenshu(BL 32) points can enhance the expression of CGT protein and mRNA in the DPN tissue? OBJECTIVE: To observe the effect of electroacupuncture at Zusanfi and Shenshu points on motor, sensory conduction velocity and CGT mRNA and its protein expression of sciatic nerve in rats with DPN. DESIGN: A randomized and controlled animal experiment SETTING : Department of Neurology and Central Laboratory, Yueyang Hospital of Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine. MATERIALS : Totally 60 healthy male Wistar rats of clean grade, aged 4 month, with body mass of 200 to 220 g, were enrolled in this study. Streptozotocin (STZ, Sigma Company of USA, Batch No. S-0130). METHODS: This study was carried out in the Animal Experimental Center and Central Laboratory, Yueyang Hospital of Traditional Chinese & Western Medicine during February 2005 to March 2006. (1) Fifteen rats were randomly chosen,serving as normal group.AU the other rats were intraperitoneally injected once with STZ to develop experimental diabetic rat models. If fasting blood glucose was ≥ 15 mmol/L,sensory nerve and motor nerve conduction velocity of sciatic nerve was obviously slowed, tail-swaying temperature threshold was increased and myelinated nerve fiber of sciatic nerve changed, DPN models were successful. The successful model rats were randomly assigned into 3 groups: model group, control group(electroacupuncture at non-meridian-non-acupoint)and electroacupuncture group [electroacupuncture at Zusan/i and Shenshu points], with 15 rats each. The rats in the normal group and model group were untouched. In the electroacupuncture group (electroacupuncture at Zusanfi and Shenshu points), Shenshu point (double) and Zusanfi point (double) were chosen referencing to The Atlas of the Rat's Acupoints. G6805- Ⅱ electric acupuncture apparatus was used, and current intensity was controlled at 20 min/time, once every other day, 12 times within 24 days. In the control group, the tip of rat-tail was stimulated, and the concrete procedures were the same as in the electroacupuncture at Zusanfi and Shenshu points. (2) Motor nerve conduction velocity and sensory nerve conduction velocity of rats were detected with neuroelectrophysiology detector in the end of the treatment, and the expressions of CGT protein and mRNA of sciatic nerve were detected with immunohistochemical method and fluorescent quantitative PCR technique. MAIN OUTCOME MEASURES: (1) Motor and sensory nerve conduction velocity. (2) The expression of CGT protein and its mRNA. RESULTS: All the 60 rats entered the stage of result analysis. (1) Comparison of motor and sensory nerve conduction velocity of rats after electroacupuncture: Motor nerve conduction velocity of rats in the model group[(31.37±3.69) m/s], control group [(32.74±5.42) m/s] and electroacupuncture group [(41.30 ±1.15) m/s] was significantly lower than that in the normal group [(41.30±1.15) m/s, P 〈 0.01]; The sensory nerve conduction velocity of rats in the model group[(18.17±9.54) m/s], control group [(21.39±5.61) m/s]and electroacupuncture group [(35.81 ±4.59) m/s] was significantly lower than that in the normal group [(46.38± 6.32) m/s,P 〈 0.01]; The motor and sensory nerve conduction velocity of electroacupuncture group was significantly higher than that in the model group [(38.04±2.01) m/s vs. (32.74±5.42) m/s,(35.81±4.59) m/s vs. (21.39±5.61) m/s,P 〈 0.01]. (2) Comparison of the expression of CGT protein of sciatic nerve of rats: The number of CGT positive cells of sciatic nerve in model group, control group or electroacupuncture group was significantly smaller than that in normal group [(9 770.33±1 461.73), (10 588.13±1119.52), (27 518.27± 9 078.29), (37 769.67±4 021.81)/μm^2,P 〈 0.01]; The number of CGT positive cells of the sciatic nerve in the electroacupuncture group was significantly larger than that in the model group and control group (P 〈 0.01). The number of CGT positive cells of sciatic nerve was close between control group and electroacupuncture group (P 〉 0.05). (3) Comparison of CGT mRNA expression of sciatic nerve of rats: Ct value of CGT mRNA of sciatic nerve of rats in the model group,control group and electroacupuncture group was significantly higher than that in the normal group (13.75±2.60,14.81±2.80,11.67±1.75,9.30±0.98, P 〈 0.01 ); Ct value of CGT mRNA of sciatic nerve of rats in the electroacupuncture group was significantly lower than that in the model group and control group (P 〈 0.01), and that was close between electroacupuncture group and control group (P 〉 0.05). CONCLUSION : Electroacupuncture at Zusanfi and Shenshu points can increase motor and sensory nerve conduction velocity of rats with DPN. It might be associated with up-regulating the expression of CGT mRNA and its protein.展开更多
OBJECTIVE:To assess the effectivess of Shenshu Guanxin recipe granules(参术冠心方颗粒,SGR)in improving exercise tolerance and the quality of life in patients with Stable Angina Pectoris(SAP).METHODS:A total of 189 pat...OBJECTIVE:To assess the effectivess of Shenshu Guanxin recipe granules(参术冠心方颗粒,SGR)in improving exercise tolerance and the quality of life in patients with Stable Angina Pectoris(SAP).METHODS:A total of 189 patients were consecutively enrolled between December 2012 and December 2014.The included patients were randomly assigned to SGR and placebo groups.The primary endpoints included mainly the results of treadmill exercise test and Seattle Angina Questionnaire(SAQ)during 12 weeks of treatment.RESULTS:After 12 weeks of treatment,SGR extended the time of exercise-induced ST-segment depression of 0.1 MV,lowered the maximum ST-segment depression,and shortened the duration of ST-segment depression in patients with SAP in southern China.Besides,the study also proved that SGR could improve the quality of life and functional status of patients with SAP.CONCLUSIONS:SGR showed a positive effect on exercise tolerance compared with the placebo besides optimal medical therapy.Also,the study proved that SGR could improve the SAQ score of the patients.展开更多
OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern con...OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.展开更多
OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying th...OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS: Half(50%) of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle(D_(min)) in the group with short-term underlying medical conditions was 38.0 mm(interquartile range 29.0–51.8 mm), approximately 6 mm deeper than 32.0(29.3–42.5) mm in the group with long-term health problems(P = 0.041). The cross-sectional area(CSA) of psoas muscle in the former group was on average approximately 1.5 times that of the latter group(P = 0.04). When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0(6.5–24.0) mm compared to 19.5(5.8–34.8) mm in the female group(P = 0.02). The age in the female group(P = 0.04) and the body length of the total group(P = 0.04) negatively correlated to D_(min).CONCLUSION: Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_(min) were observed in groups with short-term and long-term underlying medical conditions.展开更多
OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu(BL 23) in ovariectomized rats and sham-operated rats.METHODS: Female Sprague-Dawley rats were divided into the following four groups(n = 12 ea...OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu(BL 23) in ovariectomized rats and sham-operated rats.METHODS: Female Sprague-Dawley rats were divided into the following four groups(n = 12 each):sham operation only(Sham), sham operation with acupuncture(Sham + ACP), ovariectomy only(OVX), and ovariectomy with acupuncture(OVX +ACP). Operations were performed at the 9 th week of age. Acupuncture of Shenshu(BL 23) was started at the 11 th week of age and conducted 3 times per week until the 26 th week of age.RESULTS: Among ovariectomized rats, the acupuncture-treated rats had significantly lower bodyweights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acupuncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra,measured by micro-computed tomography, after17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX + ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats.CONCLUSION: Acupuncture of Shenshu(BL 23)prevents bone loss and structural changes by suppressing bone resorption.展开更多
基金the National Nat-ural Science Foundation of Chi-na, No. 30472238 the Grantfrom Bureau of Public Health ofShanghai City, No. 05JC14053Shanghai Key Subjects Con-struction Program, No. T0302
文摘BACKGROUND: Ceramide galactosyltransferase (CGT) protein and mRNA expression defect can cause the abnormal morphology and slowing conduction velocity of peripheral nerve. Morphologic change and functional disorder of myelin sheath and axon appear when diabetic peripheral neuropathy (DPN) occurs. Whether electroacupuncture at Zusanfi(ST 36) and Shenshu(BL 32) points can enhance the expression of CGT protein and mRNA in the DPN tissue? OBJECTIVE: To observe the effect of electroacupuncture at Zusanfi and Shenshu points on motor, sensory conduction velocity and CGT mRNA and its protein expression of sciatic nerve in rats with DPN. DESIGN: A randomized and controlled animal experiment SETTING : Department of Neurology and Central Laboratory, Yueyang Hospital of Traditional Chinese & Western Medicine, Shanghai University of Traditional Chinese Medicine. MATERIALS : Totally 60 healthy male Wistar rats of clean grade, aged 4 month, with body mass of 200 to 220 g, were enrolled in this study. Streptozotocin (STZ, Sigma Company of USA, Batch No. S-0130). METHODS: This study was carried out in the Animal Experimental Center and Central Laboratory, Yueyang Hospital of Traditional Chinese & Western Medicine during February 2005 to March 2006. (1) Fifteen rats were randomly chosen,serving as normal group.AU the other rats were intraperitoneally injected once with STZ to develop experimental diabetic rat models. If fasting blood glucose was ≥ 15 mmol/L,sensory nerve and motor nerve conduction velocity of sciatic nerve was obviously slowed, tail-swaying temperature threshold was increased and myelinated nerve fiber of sciatic nerve changed, DPN models were successful. The successful model rats were randomly assigned into 3 groups: model group, control group(electroacupuncture at non-meridian-non-acupoint)and electroacupuncture group [electroacupuncture at Zusan/i and Shenshu points], with 15 rats each. The rats in the normal group and model group were untouched. In the electroacupuncture group (electroacupuncture at Zusanfi and Shenshu points), Shenshu point (double) and Zusanfi point (double) were chosen referencing to The Atlas of the Rat's Acupoints. G6805- Ⅱ electric acupuncture apparatus was used, and current intensity was controlled at 20 min/time, once every other day, 12 times within 24 days. In the control group, the tip of rat-tail was stimulated, and the concrete procedures were the same as in the electroacupuncture at Zusanfi and Shenshu points. (2) Motor nerve conduction velocity and sensory nerve conduction velocity of rats were detected with neuroelectrophysiology detector in the end of the treatment, and the expressions of CGT protein and mRNA of sciatic nerve were detected with immunohistochemical method and fluorescent quantitative PCR technique. MAIN OUTCOME MEASURES: (1) Motor and sensory nerve conduction velocity. (2) The expression of CGT protein and its mRNA. RESULTS: All the 60 rats entered the stage of result analysis. (1) Comparison of motor and sensory nerve conduction velocity of rats after electroacupuncture: Motor nerve conduction velocity of rats in the model group[(31.37±3.69) m/s], control group [(32.74±5.42) m/s] and electroacupuncture group [(41.30 ±1.15) m/s] was significantly lower than that in the normal group [(41.30±1.15) m/s, P 〈 0.01]; The sensory nerve conduction velocity of rats in the model group[(18.17±9.54) m/s], control group [(21.39±5.61) m/s]and electroacupuncture group [(35.81 ±4.59) m/s] was significantly lower than that in the normal group [(46.38± 6.32) m/s,P 〈 0.01]; The motor and sensory nerve conduction velocity of electroacupuncture group was significantly higher than that in the model group [(38.04±2.01) m/s vs. (32.74±5.42) m/s,(35.81±4.59) m/s vs. (21.39±5.61) m/s,P 〈 0.01]. (2) Comparison of the expression of CGT protein of sciatic nerve of rats: The number of CGT positive cells of sciatic nerve in model group, control group or electroacupuncture group was significantly smaller than that in normal group [(9 770.33±1 461.73), (10 588.13±1119.52), (27 518.27± 9 078.29), (37 769.67±4 021.81)/μm^2,P 〈 0.01]; The number of CGT positive cells of the sciatic nerve in the electroacupuncture group was significantly larger than that in the model group and control group (P 〈 0.01). The number of CGT positive cells of sciatic nerve was close between control group and electroacupuncture group (P 〉 0.05). (3) Comparison of CGT mRNA expression of sciatic nerve of rats: Ct value of CGT mRNA of sciatic nerve of rats in the model group,control group and electroacupuncture group was significantly higher than that in the normal group (13.75±2.60,14.81±2.80,11.67±1.75,9.30±0.98, P 〈 0.01 ); Ct value of CGT mRNA of sciatic nerve of rats in the electroacupuncture group was significantly lower than that in the model group and control group (P 〈 0.01), and that was close between electroacupuncture group and control group (P 〉 0.05). CONCLUSION : Electroacupuncture at Zusanfi and Shenshu points can increase motor and sensory nerve conduction velocity of rats with DPN. It might be associated with up-regulating the expression of CGT mRNA and its protein.
基金Guangdong Medical Foundation:Exploring the Mechanism of Shenshu Guanxin Formula in Intervention of Myocardial Ischemia Based on MiR-24 Mediated Bim/Caspase Apoptosis Signal Pathway (No.A2021349)the Fundamental Research Funds for the Central Universities:Exploring the Intervention of Shenshu Guanxin Formula on Myocardial Ischemia Based on MiR-24 Mediated Bim/Caspase Apoptosis Signal Pathway Mechanism Research (No.21621062)the Science and Technology Innovation Projects of Shenzhen:to Study the Mechanism of Hesperidin in Improving Heart Failure Based on Myocardial Inflammation Mediated by Mt DNA-s GAS-STING Signaling Pathway (No.JCYJ20220530144212026)
文摘OBJECTIVE:To assess the effectivess of Shenshu Guanxin recipe granules(参术冠心方颗粒,SGR)in improving exercise tolerance and the quality of life in patients with Stable Angina Pectoris(SAP).METHODS:A total of 189 patients were consecutively enrolled between December 2012 and December 2014.The included patients were randomly assigned to SGR and placebo groups.The primary endpoints included mainly the results of treadmill exercise test and Seattle Angina Questionnaire(SAQ)during 12 weeks of treatment.RESULTS:After 12 weeks of treatment,SGR extended the time of exercise-induced ST-segment depression of 0.1 MV,lowered the maximum ST-segment depression,and shortened the duration of ST-segment depression in patients with SAP in southern China.Besides,the study also proved that SGR could improve the quality of life and functional status of patients with SAP.CONCLUSIONS:SGR showed a positive effect on exercise tolerance compared with the placebo besides optimal medical therapy.Also,the study proved that SGR could improve the SAQ score of the patients.
文摘OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.
文摘OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS: Half(50%) of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle(D_(min)) in the group with short-term underlying medical conditions was 38.0 mm(interquartile range 29.0–51.8 mm), approximately 6 mm deeper than 32.0(29.3–42.5) mm in the group with long-term health problems(P = 0.041). The cross-sectional area(CSA) of psoas muscle in the former group was on average approximately 1.5 times that of the latter group(P = 0.04). When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0(6.5–24.0) mm compared to 19.5(5.8–34.8) mm in the female group(P = 0.02). The age in the female group(P = 0.04) and the body length of the total group(P = 0.04) negatively correlated to D_(min).CONCLUSION: Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_(min) were observed in groups with short-term and long-term underlying medical conditions.
基金Supported by"High-Tech Research Center"Project for Private Universities:Matching Fund Subsidy from Ministry of Education,Culture,Sports,Science,and Technology(No.2006-2010)
文摘OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu(BL 23) in ovariectomized rats and sham-operated rats.METHODS: Female Sprague-Dawley rats were divided into the following four groups(n = 12 each):sham operation only(Sham), sham operation with acupuncture(Sham + ACP), ovariectomy only(OVX), and ovariectomy with acupuncture(OVX +ACP). Operations were performed at the 9 th week of age. Acupuncture of Shenshu(BL 23) was started at the 11 th week of age and conducted 3 times per week until the 26 th week of age.RESULTS: Among ovariectomized rats, the acupuncture-treated rats had significantly lower bodyweights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acupuncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra,measured by micro-computed tomography, after17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX + ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats.CONCLUSION: Acupuncture of Shenshu(BL 23)prevents bone loss and structural changes by suppressing bone resorption.