咪喹莫特对BALB/c和C57BL/6小鼠银屑病样皮损诱导作用的比较

目的观察咪喹莫特对不同品系小鼠银屑病样皮损诱导作用的差异及其作用机理。方法选用BALB/c和C57BL/6两种小鼠品系,分别在其背部涂抹62.5 mg含5%咪喹莫特的明欣利迪乳膏,在涂抹2,4,6 d后,采用PAIS评分观察小鼠背部皮肤形态学变化;HE染色观察涂药局部皮肤病理组织学变化,显微镜下测量表皮厚度;实时荧光定量PCR法测定IL-23/IL-17轴相关细胞因子的相对表达量。结果随咪喹莫特涂抹天数的增加,BALB/c小鼠和C57BL/6小鼠背部皮肤均逐渐出现鳞屑、红斑、表皮增厚等银屑病样表现。与同时间段C57BL/6小鼠银屑病样皮损比较,BALB/c小鼠皮损PAIS评分更高(P<0.05或P<0.01),病理组织学改变及表皮增厚均更显著(P<0.05或P<0.01)。涂抹咪喹莫特2 d后,两种小鼠IL-23、IL-17A和IL-17F mRNA相对表达量均显著升高,且均呈现先高后低的动态变化。其中BALB/c小鼠表达峰值出现于涂药后2 d,C57BL/6小鼠表达峰值出现于涂药后4 d。结论咪喹莫特均可诱导BALB/c和C57BL/6小鼠出现人类银屑病样皮损,其中BALB/c小鼠银屑病样皮损更典型,可能更适用于人类银屑病研究,IL-23/IL-17轴的激活是皮损出现的重要诱导因素之一。

HSF2、IL-23、MDC在溃疡性结肠炎病情评估中的应用价值

【目的】探讨热休克转录因子2(HSF2)、白细胞介素-23(IL-23)、髓样树突状细胞(MDC)在溃疡性结肠炎(UC)病情评估中的应用价值。【方法】选取2019年6月至2021年8月本院收治的146例UC患者(观察组),另选取73例同期健康体检志愿者作为对照组,比较观察组入院时、对照组体检时及不同病情UC患者血清HSF2、IL-23、MDC水平,采用Pearson分析上述指标与患者病情相关性,采用受试者工作特征曲线(ROC曲线)分析其对UC的诊断价值。【结果】观察组血清HSF2、IL-23、MDC水平高于对照组,差异有统计学意义(P<0.05)。活动期组患者血清HSF2、IL-23、MDC水平高于缓解期组,差异有统计学意义(P<0.05)。三组患者血清HSF2、IL-23、MDC水平比较,重度组>中度组>轻度组,差异有统计学意义(P<0.05)。Pearson相关性分析显示,血清HSF2、IL-23、MDC水平与发作时期、病情程度均呈正相关(均P<0.05)。ROC曲线分析显示,血清HSF2、IL-23、MDC水平对UC诊断的曲线下面积(AUC)分别为0.795、0.832、0.856,联合诊断AUC为0.927,大于单一指标(P<0.05)。【结论】血清HSF2、IL-23、MDC水平对UC患者病情严重程度具有一定评估价值,可为临床早期诊断UC提供参考。

Effect of moxibustion at Shenshu(BL 23) on the ethology,corticosterone and glucocorticoid receptor in aging rats

Objective：To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor（GR） in subacute aging rats.Methods：Twenty four Sprague-Dawley（SD） rats were randomly divided into a normal group,a model group and a moxibustion group,8 rats in each group.Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/（kg·bw）] for 40 d continuous;rats in the normal group were injected with saline at the same position for 40 d continuous.Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu（BL 23） at the same time of modeling;rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d.The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment.Abdominal aorta blood and thymus were collected after water maze experiment.Enzyme-linked immunosorbent assay（ELISA） was used to detect serum corticosterone level,and immunohistochemical method was used to detect the expression of thymus GR.Results：Compared with the normal group,rats in the model group showed that a significantly longer escape latency time（P〈0.01） on the third and the fourth days;number of times crossing the platform in 70 s significantly reduced（P〈0.01）;activity times in the fourth quadrant significantly decreased（P〈0.05）;serum corticosterone levels increased（P〈0.01）;thymus GR expression decreased（P〈0.05）.Compared with the model group,rats in the moxibustion group showed that the escape latency times were significantly shorter on the third,the fourth and the fifth days（P〈0.01,P〈0.05）;number of times crossing the platform in 70 s significantly increased（P〈0.05）;activity times in the fourth quadrant significantly increased（P〈0.05）;serum corticosterone levels decreased（P〈0.05）;thymus GR expression increased（P〈0.05）.Conclusion：Moxibustion could improve the learning and memory abilities of subacute aging rats,down-regulate serum corticosterone levels,and increase thymus GR content.

Effect of electroacupuncture at Ganshu(BL 18) and Shenshu(BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat

Objective： To observe the effect of electroacupuncture （EA） on the expression of erythropoie-tin-producing hepatocyte receptor B2 （EphB2） in the cortex around the infracted area of middle cerebral artery occlusion （MCAO） rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods： A total of 180 male Sprague-Dawley （SD） rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups： postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride （TFC） staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results： The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group （P〈0.05）. The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells （EphB2＋） around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group （P〈0.05）, and the positive cell number was higher than that in the sham operation group （P〈0.01）. Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2＋ and postsynaptic density-95 positive （PSD-95＋） were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group （P〈0.05）. Conclusion： Electroacupuncture at Ganshu （BL 18） and Shenshu （BL 23） can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.

针灸治疗急性肾绞痛临床选穴组方规律研究

【目的】通过数据挖掘技术分析临床针灸治疗急性肾绞痛选穴组方的规律。【方法】检索中国知网期刊全文数据库(CNKI)、万方学术期刊全文数据库(Wanfang)、维普中文科技期刊数据库(VIP)、PubMed数据库已经公开发表并收录的关于针灸治疗急性肾绞痛的临床文献,通过严格筛选,采用Microsoft Excel、SPSS 26.0、IBM SPSS Modeler 15.0软件以及其中的Apriori算法对纳入文献进行信息挖掘与分析。【结果】纳入110篇关于针灸治疗急性肾绞痛的临床文献,统计针灸处方共110条,涉及使用腧穴共59个,其中,肾俞、阿是穴和三阴交的使用频次最高;常用经脉为足太阳膀胱经、足太阴脾经、足阳明胃经;腧穴主要分布在下肢与腰背部;从选用穴位类型来看,五输穴、背俞穴、下合穴为首选;双穴组合配伍中,肾俞、阿是穴组合支持度最高;三穴组合配伍中,肾俞、三阴交、阿是穴组合支持度最高;核心腧穴组合是肾俞与阿是穴组合。【结论】针灸治疗急性肾绞痛的腧穴选择和组方是有规律可循的,所总结的选穴和组方规律能够为其临床治疗和科学研究提供参考。

Effect of acupuncture at acupoint of Shenshu (BL 23) on the bones of ovariectomized rats

OBJECTIVE： To investigate the effect of acupuncture at acupoint of Shenshu （BL 23） in ovariectomized rats and sham-operated rats. METHODS： Female Sprague-Dawley rats were divided into the following four groups （n = 12 each）： sham operation only （Sham）, sham operation with acupuncture （Sham ＋ ACP）, ovariectomy only （OVX）, and ovariectomy with acupuncture （OVX ＋ ACP）. Operations were performed at the 9th week of age. Acupuncture of Shenshu （BL 23） was started at the 1 lth week of age and conducted 3 times per week until the 26th week of age. RESULTS： Among ovariectomized rats, the acupuncture-treated rats had significantly lower body weights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acu- puncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra, measured by micro-computed tomography, after 17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX ＋ ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats. CONCLUSION： Acupuncture of Shenshu （BL 23） prevents bone loss and structural changes by sup- pressing bone resorption.

Specific acupuncture stimulation of Shenshu(BL23)affects sympathetic nervous activity-associated plasma renin concentration changes

OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.

A study to investigate needle insertion at Shenshu(BL23) to puncture psoas major muscle

OBJECTIVE： It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu（BL23）.METHODS： Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS： Half（50%） of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle（D_（min）） in the group with short-term underlying medical conditions was 38.0 mm（interquartile range 29.0–51.8 mm）, approximately 6 mm deeper than 32.0（29.3–42.5） mm in the group with long-term health problems（P = 0.041）. The cross-sectional area（CSA） of psoas muscle in the former group was on average approximately 1.5 times that of the latter group（P = 0.04）. When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0（6.5–24.0） mm compared to 19.5（5.8–34.8） mm in the female group（P = 0.02）. The age in the female group（P = 0.04） and the body length of the total group（P = 0.04） negatively correlated to D_（min）.CONCLUSION： Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_（min） were observed in groups with short-term and long-term underlying medical conditions.

温针灸“肾俞”穴对老年雌性大鼠性激素的影响

目的 :观察温针灸对老年雌性大鼠性激素的影响。方法 :温针灸“肾俞”穴 ,观察其对雌二醇(E2 )、孕酮 (P)、促卵泡生成激素 (FSH)、促黄体生成激素 (LH)的变化。结果 :温针灸“肾俞”组E2 水平明显升高 ,温针灸“肾俞”与尼尔雌醇组FSH水平明显降低 (P <0 0 1) ,P显著升高 (P <0 0 5,P<0 0 1)。温针灸“肾俞”对LH水平的变化影响不大 ,而尼尔雌醇组LH水平则明显低于空白对照组。结论 :温针灸“肾俞”穴具有明显的调节性激素作用 ,可以使明显低下的雌二醇与孕酮水平升高 ,从而建立起卵巢与垂体之间负反馈关系 ,同时 。

肾俞募穴与肾特异性联系通路的荧光双标法研究

目的 :探求肾与其俞募穴之间的神经联系通路。方法 :应用荧光双标记法进行研究。结果 :“肾俞” 肾组、“京门” 肾组、“肾俞” “京门”组分别在T1 3～L3、T1 2 ～L2 、T1 3～L1 脊神经节内出现双标细胞。结论 :①脊神经节中存在可双重支配肾和体表的神经元 ,不仅使牵涉痛的机制得到进一步的解释 ,而且揭示了针刺穴位的感觉冲动是通过分支的传入轴突影响到内脏的功能和感觉或对某些脏器的活动产生影响。②为临床应用肾俞募穴治疗肾病提供了形态学理论根据。③进而揭示中医经络学中气街可能的现代生物学本质。

电针肾俞、足三里治疗慢性疲劳综合征的随机对照研究

目的观察电针肾俞、足三里治疗慢性疲劳综合征的疗效。方法将60例慢性疲劳综合征患者随机分为电针穴位组和电针非穴位组各30例,治疗2个疗程;应用疲劳严重程度量表(FSS)、躯体及心理健康报告(SPHERE)、疼痛视觉模拟量表(VAS)以及健康状况调查简表(SF-36)评定患者疲劳程度、潜在症状、疼痛程度以及生活质量。结果治疗后两组FSS、SPHERE、VAS量表积分降低,SF-36各维度积分均明显升高(P<0.01),且电针穴位组明显优于非穴位组(P<0.01);治疗结束后1个月、3个月随访,患者FSS积分、SPHERE积分以及VAS积分较治疗后均有所升高,但电针穴位组低于电针非穴位组(P<0.01),SF-36各维度积分较治疗后下降,除总体健康维度外其他维度积分电针穴位组高于电针非穴位组(P<0.01)。结论电针肾俞、足三里能明显减轻CFS患者的临床症状,提高其生活质量。

针灸“肾俞”穴对吗啡依赖大鼠戒断反应和成瘾脑区神经元活动的影响

目的 :探讨电针肾俞穴对吗啡戒断的影响及其作用机制。方法 :建立急性吗啡成瘾模型 ,采用瞬时基因c fos表达作为神经元活动的标志 ,运用免疫组化技术 ,对电针“肾俞”穴对抗吗啡戒断症状以及可能涉及的脑区进行研究。结果 :电针“肾俞”穴能明显抑制大鼠戒断症状 ,降低导水管周围灰质区、下丘脑室旁核、海马CA1区、CA3区和齿状回和下丘脑外侧区的c fos表达 ,但增加杏仁基底外侧核、杏仁中央核和伏隔核核区 (core)的c fos表达。结论 :电针“肾俞”穴能有效抑制吗啡戒断症状 ,电针对抗阿片类药物依赖涉及多个中枢核团。

电针对慢性疲劳综合征病理节律的调整作用

目的:探讨电针对慢性疲劳综合征(CFS)病理节律的调整作用。方法:20例CFS患者与20例健康受试者进行对照,CFS患者采用电针肾俞、足三里进行治疗。应用时间生物学的研究方法对患者治疗前后以及健康受试者进行24h核心体温、心率、血压、唾液皮质醇和褪黑素的动态检测。结果:余弦法分析发现,健康组和CFS组治疗后各项指标都具有昼夜节律(P<0.05);CFS组治疗前舒张压昼夜节律消失(P>0.05),其余指标具有昼夜节律(P<0.05),但CFS组治疗前昼夜节律振幅低于健康对照组,且核心体温、心率、血压和褪黑素峰值相位后移而皮质醇峰值相位超前;电针治疗后,CFS组振幅明显提高,峰值相位接近于健康对照组(P>0.05)。结论:CFS存在昼夜节律紊乱现象,电针对其具有良性调整作用。

肾俞埋线对绝经后骨质疏松症临床疼痛的影响

目的探讨肾俞穴位埋线治疗绝经后骨质疏松症临床疼痛的有效性。方法将56例患者随机分为埋线组、埋线乐力组和乐力组,以疼痛评分方法评定治疗前、治疗3个月和治疗6个月疼痛情况。结果埋线组、埋线乐力组疼痛评分于治疗前后比较,差异均有极显著性意义(P<0·001),两组间比较,差异无显著性意义(P>0·05);乐力组疼痛评分于治疗前后比较,差异无显著性意义(P>0·05),与埋线组、埋线乐力组分别比较,差异均有极显著性意义(P<0·001)。结论肾俞穴位埋线对绝经后骨质疏松症临床疼痛有非常明显的疗效;口服乐力胶囊对改善绝经后骨质疏松症临床疼痛无显著疗效。

“足三里”和“肾俞”穴位埋线对大鼠吗啡镇痛耐受和运动行为敏化的影响

目的:观察"足三里"和"肾俞"穴位埋线对大鼠吗啡镇痛耐受和行为敏化的效应差异,探讨其作用机制。方法:大鼠随机分为模型组、非穴位点组、肾俞组、足三里组。除模型组外,其余3组均于注射吗啡10d前开始行穴位埋线。建立慢性吗啡耐受模型,每天应用热板试验测定痛阈。并在第1次吗啡注射,以及吗啡停药1w后再激发进行活动度检测。应用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组织化学显示一氧化氮合酶阳性神经元。结果:与非穴位点组相比,"足三里"埋线减缓慢性吗啡处理的痛阈下降和减少大鼠活动度,伏隔核和背侧纹状体内一氧化氮合酶阳性神经元表达减少;肾俞组痛阈和活动度较非穴位点组均未见明显差异,而背侧纹状体区一氧化氮合酶阳性神经元表达明显减少。结论:穴位埋线于"足三里"可减缓吗啡镇痛耐受,逆转吗啡行为敏化形成,其调节可能与抑制伏隔核和背侧纹状体区一氧化氮合酶表达有关。

电针对前列腺增生症患者夜间尿频的影响

目的观察电针治疗良性前列腺增生症患者夜间尿频症状的临床疗效。方法对48例良性前列腺增生症患者采用电针肾俞、会阳穴进行治疗,观察治疗前后国际前列腺症状评分(I-PSS)、生活质量评分(QOL)、残余尿量(PVR)及夜间多尿指数(NPi)变化,并着重观察其对夜尿次数的影响。结果 48例患者治疗后I-PSS、QOL、PVR、夜尿次数及NPi均减少,其中I-PSS、QOL、PVR、夜尿次数与治疗前比较,差异均具有统计学意义(P<0.01)。结论电针肾俞、会阳穴可有效改善良性前列腺增生症患者的夜间尿频症状。

电针对不稳定膀胱影响的穴位特异性研究

目的:研究不同穴位电针治疗不稳定膀胱的尿动力学改变及其特异性。方法:将60只大鼠随机分为肾俞组、会阳组、肾俞加会阳组、药物组、模型组、假手术组,并给予相应处理;治疗12h后观察各组逼尿肌压力、膀胱顺应性、膀胱容量的变化。结果:各治疗方法均可改善USB大鼠的尿动力学状态;各治疗组尿动力改善情况较模型组显著(P<0.05),且各穴位组尿动力改善与药物组相似;会阳组、肾俞加会阳组对不稳定膀胱大鼠尿动力学改善优于药物组、肾俞组(P<0.05);会阳组与肾俞加会阳组之间无明显差异。结论:会阳穴、会阳加肾俞穴可特异性地改善USB尿动力状态,抑制逼尿肌不稳定收缩,增加膀胱容量和膀胱顺应性。

大鼠肾俞定位之我见

肾俞主治病症多,临床应用广泛,因此对于肾俞穴的实验研究层出不穷。大鼠作为实验常用动物,其腧穴的准确定位对于针灸实验研究具有重要的意义。本研究在阐明大鼠脊椎解剖特点的基础上,指明大鼠肾俞的定位方法,希望对针灸实验研究能起到一定的指导作用。

俞募穴埋线治疗卵巢早衰疗效观察

目的:观察肝脾肾之俞募穴埋线治疗卵巢早衰的临床疗效。方法:将132例卵巢早衰患者随机分成埋线组和药物组,每组各66例。埋线组给予肝脾肾之俞募穴埋线治疗;药物组采用激素替代疗法,即口服戊酸雌二醇(补佳乐)2 mg,每日1次,连服20天,10天后加服安宫黄体酮4 mg,每日2次,连服10天,平均用药6个月。观察2组疗效及血清促卵泡激素(FSH)和雌二醇(E2)变化。结果:2组治疗后血清FSH和E2均有显著改善(P<0.01),且埋线组血清E2提高较药物组显著(P<0.01)。埋线组痊愈率为84.9%,总有效率为97.0%;药物组分别为31.8%、84.8%。埋线组痊愈率优于药物组(P<0.05)。随访10个月,埋线组疗效可维持。结论:肝脾肾之俞募穴埋线治疗卵巢早衰疗效好,无任何毒副作用,值得临床推广应用。

萸桂散穴位贴敷治疗Ⅳ期糖尿病肾病40例

目的:观察萸桂散穴位贴敷治疗Ⅳ期糖尿病肾病的临床疗效。方法:将80例糖尿病肾病Ⅳ期患者随机分为治疗组及对照组,每组40例。对照组给予基础护理、西医基础治疗、中药汤剂内服,治疗组在对照组的基础上使用萸桂散贴敷双涌泉及双肾俞。比较两组患者的临床疗效,观察两组患者治疗前后糖化血红蛋白(glycosylated hemoglobin,Hb A1c)、肌酐(serum creatinine,Scr)、尿微量白蛋白与肌酐比值(ACR)及尿白蛋白排泄率(urinary albumin excretion rate,UAER)改善情况。结果:对照组有效率为60.0%,治疗组有效率为90.0%,两组患者临床疗效比较,差异具有统计学意义(P<0.05)。两组患者治疗后ACR较治疗前下降,差异具有统计学意义(P<0.05),但治疗组与对照组治疗后比较,差异无统计学意义(P>0.05);两组治疗后的CREA和Hb A1c水平较治疗前均有所下降,但差异无统计学意义(P>0.05)。两组肌酐水平正常的患者治疗后ACR水平较治疗前下降,差异具有统计学意义(P<0.05),但治疗组与对照组治疗后比较,差异无统计学意义(P>0.05);两组治疗后的CREA和Hb A1c水平较治疗前均有所下降,但差异无统计学意义(P>0.05)。结论:萸桂散穴位贴敷治疗Ⅳ期糖尿病肾病可有效缓解临床症状、降低ACR。