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加工型马铃薯品种Shepody农杆菌介导的转化体系研究 被引量:4
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作者 辛翠花 黄三文 +3 位作者 郭江波 刘杰 冷小云 屈冬玉 《中国农学通报》 CSCD 北大核心 2011年第21期87-92,共6页
马铃薯Shepody是经济价值很高的炸条加工品种,但Shepody对逆境胁迫非常敏感,为确立Shepody的遗传转化体系并获得转基因马铃薯,以叶片、茎段和微型薯为外植体,分别比较了不同转化条件(菌液浓度、侵染时间及激素配比)对遗传转化效率的影... 马铃薯Shepody是经济价值很高的炸条加工品种,但Shepody对逆境胁迫非常敏感,为确立Shepody的遗传转化体系并获得转基因马铃薯,以叶片、茎段和微型薯为外植体,分别比较了不同转化条件(菌液浓度、侵染时间及激素配比)对遗传转化效率的影响。结果表明:叶片为最理想的外植体,以OD600=0.5的菌液侵染10min,在加有1.0mg/L萘乙酸(NAA)和1.0mg/L反式玉米素(ZT-t)的MS20选择培养基上的遗传转化率最高,其愈伤诱导率可达到95%,分化率可达到20%。通过该转化体系将抗晚疫病基因R1导入马铃薯,获得了具有卡那霉素抗性的转化植株,经PCR鉴定外源基因已经成功导入马铃薯基因组中。 展开更多
关键词 马铃薯 shepody 遗传转化 外植体
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The Application of K Phosphites to Seed Tubers Enhanced Emergence, Early Growth and Mycorrhizal Colonization in Potato (<i>Solanum tuberosum</i>) 被引量:7
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作者 Cecilia Tambascio Fernanda Covacevich +4 位作者 María Candela Lobato Carolina de Lasa Daniel Caldiz Guillermo Dosio Adriana Andreu 《American Journal of Plant Sciences》 2014年第1期132-137,共6页
Rapid emergence and a vigorous growth prevent the seed tubers from infections by soil microbes and allow a rapid interception of solar radiation. In this work, the effect of the potassium phosphites (KPhi) applied to ... Rapid emergence and a vigorous growth prevent the seed tubers from infections by soil microbes and allow a rapid interception of solar radiation. In this work, the effect of the potassium phosphites (KPhi) applied to seed tubers of two potato cultivars on crop emergence and early growth was studied. Two experiments were performed under greenhouse and field conditions. Emergence of plants, leaf area, dry matter and the number of primary stems were measured in both experiments. Furthermore, mycorrhizal colonization was also measured on roots under field conditions. The application of KPhi reduced the period between planting and emergence, and increased leaf area and dry matter. The ratio between dry matter of aerials and underground organs was not affected by KPhi. Indigenous mycorrhizal colonization increased after KPhi application to seed tubers. These results confirm the benefit of the application of KPhi to seed tubers on early plant growth and suggest that their application in crop production would be advantageous. 展开更多
关键词 Seed TUBERS Leaf Area Dry Matter CULTIVAR Kennebec CULTIVAR shepody
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