Background:Withania somnifera(WS)is proposed as one of the alternatives instead of the antibiotic.This study is aimed to evaluate the inhibitory potency of enzymatic extract of the fruits of the WS.Methods:As an invit...Background:Withania somnifera(WS)is proposed as one of the alternatives instead of the antibiotic.This study is aimed to evaluate the inhibitory potency of enzymatic extract of the fruits of the WS.Methods:As an invitro experimental study,the growth rate of Shigella dysenteriae,Salmonella typhimurium,and Escherichia coli inoculated in different concentrations(25%,12.5%,6.25%and 3.125%)of the extract were assessed.A microtitre plate method was conducted.ANOVA was applied to identify statistical differences with p-value<0.05.Results:Different concentrations of extract,in comparison with control,declined the growth rate of all tested bacteria.All concentrations inhibited the growth of S.typhimurium(p<0.05).Compared to the microorganism control,effective concentration of the extract inhibiting the growth of E.coli was 12.5%,and 6.25%,while it was 12.5%,and 6.25%for Sh.dysenteriae(p<0.05).A dose-dependent response of E.coli was observed.The antibacterial activity of the extract tested was found mainly against E.coli and Sh.dysenteriae.The most resistant microorganism compared to E.coli and Sh.dysenteriae was S.typhimurium(p<0.05).25%of the concentration of the extract showed the different inhibitory effect among three tested bacteria(p<0.05).Conclusions:The extract was labeled as an antibacterial agent against the representative of three foodborne bacteria,Invitro.The common effective concentrations of the extract(12.5,and 6.25%)is recommended for further research,as food additive,to remedy digestive ailments related to E.coli,S.typhimurium and Sh.dysenteriae.展开更多
文摘Background:Withania somnifera(WS)is proposed as one of the alternatives instead of the antibiotic.This study is aimed to evaluate the inhibitory potency of enzymatic extract of the fruits of the WS.Methods:As an invitro experimental study,the growth rate of Shigella dysenteriae,Salmonella typhimurium,and Escherichia coli inoculated in different concentrations(25%,12.5%,6.25%and 3.125%)of the extract were assessed.A microtitre plate method was conducted.ANOVA was applied to identify statistical differences with p-value<0.05.Results:Different concentrations of extract,in comparison with control,declined the growth rate of all tested bacteria.All concentrations inhibited the growth of S.typhimurium(p<0.05).Compared to the microorganism control,effective concentration of the extract inhibiting the growth of E.coli was 12.5%,and 6.25%,while it was 12.5%,and 6.25%for Sh.dysenteriae(p<0.05).A dose-dependent response of E.coli was observed.The antibacterial activity of the extract tested was found mainly against E.coli and Sh.dysenteriae.The most resistant microorganism compared to E.coli and Sh.dysenteriae was S.typhimurium(p<0.05).25%of the concentration of the extract showed the different inhibitory effect among three tested bacteria(p<0.05).Conclusions:The extract was labeled as an antibacterial agent against the representative of three foodborne bacteria,Invitro.The common effective concentrations of the extract(12.5,and 6.25%)is recommended for further research,as food additive,to remedy digestive ailments related to E.coli,S.typhimurium and Sh.dysenteriae.
