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Study on the Uncertainty of the Available Time Under Ship Fire Based on Monte Carlo Sampling Method 被引量:2
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作者 汪金辉 褚冠全 李开源 《China Ocean Engineering》 SCIE EI CSCD 2013年第1期131-140,共10页
Available safety egress time under ship fire (SFAT) is critical to ship fire safety assessment, design and emergency rescue. Although it is available to determine SFAT by using fire models such as the two-zone fire ... Available safety egress time under ship fire (SFAT) is critical to ship fire safety assessment, design and emergency rescue. Although it is available to determine SFAT by using fire models such as the two-zone fire model CFAST and the field model FDS, none of these models can address the uncertainties involved in the input parameters. To solve this problem, current study presents a framework of uncertainty analysis for SFAT. Firstly, a deterministic model estimating SFAT is built. The uncertainties of the input parameters are regarded as random variables with the given probability distribution functions. Subsequently, the deterministic SFAT model is employed to couple with a Monte Carlo sampling method to investigate the uncertainties of the SFAT. The Spearman's rank-order correlation coefficient (SRCC) is used to examine the sensitivity of each input uncertainty parameter on SFAT. To illustrate the proposed approach in detail, a case study is performed. Based on the proposed approach, probability density function and cumulative density function of SFAT are obtained. Furthermore, sensitivity analysis with regard to SFAT is also conducted. The results give a high-negative correlation of SFAT and the fire growth coefficient whereas the effect of other parameters is so weak that they can be neglected. 展开更多
关键词 ship fire ship fire available time Monte Carlo sampling uncertainty analysis sensitivity analysis
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Fault Tree Analysis of Fire and Explosion Accidents for Dual Fuel (Diesel/Natural Gas) Ship Engine Rooms 被引量:1
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作者 Yifeng Guan Jie Zhao +1 位作者 Tengfei Shi Peipei Zhu 《Journal of Marine Science and Application》 CSCD 2016年第3期331-335,共5页
In recent years, China's increased interest in environmental protection has led to a promotion of energy-efficient dual fuel(diesel/natural gas) ships in Chinese inland rivers. A natural gas as ship fuel may pose ... In recent years, China's increased interest in environmental protection has led to a promotion of energy-efficient dual fuel(diesel/natural gas) ships in Chinese inland rivers. A natural gas as ship fuel may pose dangers of fire and explosion if a gas leak occurs. If explosions or fires occur in the engine rooms of a ship, heavy damage and losses will be incurred. In this paper, a fault tree model is presented that considers both fires and explosions in a dual fuel ship; in this model, dual fuel engine rooms are the top events. All the basic events along with the minimum cut sets are obtained through the analysis.The primary factors that affect accidents involving fires and explosions are determined by calculating the degree of structure importance of the basic events.According to these results, corresponding measures are proposed to ensure and improve the safety and reliability of Chinese inland dual fuel ships. 展开更多
关键词 dual fuel ship engine room fault tree fire and explosion safety measures gas leak
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A Time-Domain Numerical Simulation for Free Motion Responses of Two Ships Advancing in Head Waves
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作者 PAN Su-yong CHENG Yong 《China Ocean Engineering》 SCIE EI CSCD 2024年第3期519-530,共12页
The constant panel method within the framework of potential flow theory in the time domain is developed for solving the hydrodynamic interactions between two parallel ships with forward speed.When solving problems wit... The constant panel method within the framework of potential flow theory in the time domain is developed for solving the hydrodynamic interactions between two parallel ships with forward speed.When solving problems within a time domain framework,the free water surface needs to simultaneously satisfy both the kinematic and dynamic boundary conditions of the free water surface.This provides conditions for adding artificial damping layers.Using the Runge−Kutta method to solve equations related to time.An upwind differential scheme is used in the present method to deal with the convection terms on the free surface to prevent waves upstream.Through the comparison with the available experimental data and other numerical methods,the present method is proved to have good mesh convergence,and satisfactory results can be obtained.The constant panel method is applied to calculate the hydrodynamic interaction responses of two parallel ships advancing in head waves.Numerical simulations are conducted on the effects of forward speed,different longitudinal and lateral distances on the motion response of two modified Wigley ships in head waves.Then further investigations are conducted on the effects of different ship types on the motion response. 展开更多
关键词 ship motions time domain simulation forward speed different distances wave loads
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Unmanned Ship Identification Based on Improved YOLOv8s Algorithm
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作者 Chun-Ming Wu Jin Lei +2 位作者 Wu-Kai Liu Mei-Ling Ren Ling-Li Ran 《Computers, Materials & Continua》 SCIE EI 2024年第3期3071-3088,共18页
Aiming at defects such as low contrast in infrared ship images,uneven distribution of ship size,and lack of texture details,which will lead to unmanned ship leakage misdetection and slow detection,this paper proposes ... Aiming at defects such as low contrast in infrared ship images,uneven distribution of ship size,and lack of texture details,which will lead to unmanned ship leakage misdetection and slow detection,this paper proposes an infrared ship detection model based on the improved YOLOv8 algorithm(R_YOLO).The algorithm incorporates the Efficient Multi-Scale Attention mechanism(EMA),the efficient Reparameterized Generalized-feature extraction module(CSPStage),the small target detection header,the Repulsion Loss function,and the context aggregation block(CABlock),which are designed to improve the model’s ability to detect targets at multiple scales and the speed of model inference.The algorithm is validated in detail on two vessel datasets.The comprehensive experimental results demonstrate that,in the infrared dataset,the YOLOv8s algorithm exhibits improvements in various performance metrics.Specifically,compared to the baseline algorithm,there is a 3.1%increase in mean average precision at a threshold of 0.5(mAP(0.5)),a 5.4%increase in recall rate,and a 2.2%increase in mAP(0.5:0.95).Simultaneously,while less than 5 times parameters,the mAP(0.5)and frames per second(FPS)exhibit an increase of 1.7%and more than 3 times,respectively,compared to the CAA_YOLO algorithm.Finally,the evaluation indexes on the visible light data set have shown an average improvement of 4.5%. 展开更多
关键词 Unmanned ships R_YOLO EMA CSPStage YOLOv8s
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Efficient Ship:A Hybrid Deep Learning Framework for Ship Detection in the River
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作者 Huafeng Chen Junxing Xue +2 位作者 Hanyun Wen Yurong Hu Yudong Zhang 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第1期301-320,共20页
Optical image-based ship detection can ensure the safety of ships and promote the orderly management of ships in offshore waters.Current deep learning researches on optical image-based ship detection mainly focus on i... Optical image-based ship detection can ensure the safety of ships and promote the orderly management of ships in offshore waters.Current deep learning researches on optical image-based ship detection mainly focus on improving one-stage detectors for real-time ship detection but sacrifices the accuracy of detection.To solve this problem,we present a hybrid ship detection framework which is named EfficientShip in this paper.The core parts of the EfficientShip are DLA-backboned object location(DBOL)and CascadeRCNN-guided object classification(CROC).The DBOL is responsible for finding potential ship objects,and the CROC is used to categorize the potential ship objects.We also design a pixel-spatial-level data augmentation(PSDA)to reduce the risk of detection model overfitting.We compare the proposed EfficientShip with state-of-the-art(SOTA)literature on a ship detection dataset called Seaships.Experiments show our ship detection framework achieves a result of 99.63%(mAP)at 45 fps,which is much better than 8 SOTA approaches on detection accuracy and can also meet the requirements of real-time application scenarios. 展开更多
关键词 ship detection deep learning data augmentation object location object classification
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Fine-Grained Ship Recognition Based on Visible and Near-Infrared Multimodal Remote Sensing Images: Dataset,Methodology and Evaluation
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作者 Shiwen Song Rui Zhang +1 位作者 Min Hu Feiyao Huang 《Computers, Materials & Continua》 SCIE EI 2024年第6期5243-5271,共29页
Fine-grained recognition of ships based on remote sensing images is crucial to safeguarding maritime rights and interests and maintaining national security.Currently,with the emergence of massive high-resolution multi... Fine-grained recognition of ships based on remote sensing images is crucial to safeguarding maritime rights and interests and maintaining national security.Currently,with the emergence of massive high-resolution multi-modality images,the use of multi-modality images for fine-grained recognition has become a promising technology.Fine-grained recognition of multi-modality images imposes higher requirements on the dataset samples.The key to the problem is how to extract and fuse the complementary features of multi-modality images to obtain more discriminative fusion features.The attention mechanism helps the model to pinpoint the key information in the image,resulting in a significant improvement in the model’s performance.In this paper,a dataset for fine-grained recognition of ships based on visible and near-infrared multi-modality remote sensing images has been proposed first,named Dataset for Multimodal Fine-grained Recognition of Ships(DMFGRS).It includes 1,635 pairs of visible and near-infrared remote sensing images divided into 20 categories,collated from digital orthophotos model provided by commercial remote sensing satellites.DMFGRS provides two types of annotation format files,as well as segmentation mask images corresponding to the ship targets.Then,a Multimodal Information Cross-Enhancement Network(MICE-Net)fusing features of visible and near-infrared remote sensing images,has been proposed.In the network,a dual-branch feature extraction and fusion module has been designed to obtain more expressive features.The Feature Cross Enhancement Module(FCEM)achieves the fusion enhancement of the two modal features by making the channel attention and spatial attention work cross-functionally on the feature map.A benchmark is established by evaluating state-of-the-art object recognition algorithms on DMFGRS.MICE-Net conducted experiments on DMFGRS,and the precision,recall,mAP0.5 and mAP0.5:0.95 reached 87%,77.1%,83.8%and 63.9%,respectively.Extensive experiments demonstrate that the proposed MICE-Net has more excellent performance on DMFGRS.Built on lightweight network YOLO,the model has excellent generalizability,and thus has good potential for application in real-life scenarios. 展开更多
关键词 Multi-modality dataset ship recognition fine-grained recognition attention mechanism
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Time-Domain Higher-Order Boundary Element Method for Simulating High Forward-Speed Ship Motions in Waves
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作者 ZHOU Xiao-guo CHENG Yong PAN Su-yong 《China Ocean Engineering》 SCIE EI CSCD 2024年第5期904-914,共11页
The hydrodynamic performance of a high forward-speed ship in obliquely propagating waves is numerically examined to assess both free motions and wave field in comparison with a low forward-speed ship.This numerical mo... The hydrodynamic performance of a high forward-speed ship in obliquely propagating waves is numerically examined to assess both free motions and wave field in comparison with a low forward-speed ship.This numerical model is based on the time-domain potential flow theory and higher-order boundary element method,where an analytical expression is completely expanded to determine the base-unsteady coupling flow imposed on the moving condition of the ship.The ship in the numerical model may possess different advancing speeds,i.e.stationary,low speed,and high speed.The role of the water depth,wave height,wave period,and incident wave angle is analyzed by means of the accurate numerical model.It is found that the resonant motions of the high forward-speed ship are triggered by comparison with the stationary one.More specifically,a higher forward speed generates a V-shaped wave region with a larger elevation,which induces stronger resonant motions corresponding to larger wave periods.The shoaling effect is adverse to the motion of the low-speed ship,but is beneficial to the resonant motion of the high-speed ship.When waves obliquely propagate toward the ship,the V-shaped wave region would be broken due to the coupling effect between roll and pitch motions.It is also demonstrated that the maximum heave motion occurs in beam seas for stationary cases but occurs in head waves for high speeds.However,the variation of the pitch motion with period is hardly affected by wave incident angles. 展开更多
关键词 high forward speed oblique incident waves ship motion higher-order boundary element method time domain wave field
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Structural Characteristics and Evolution of a Weighted Sino-US Container Shipping Network
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作者 ZHANG Tiantian XI Daping +3 位作者 JIANG Wenping FENG Yuhao WANG Chuyuan HU Xini 《Chinese Geographical Science》 SCIE CSCD 2024年第5期810-828,共19页
This study selected the Sino-US route data from the top 30 global container liner companies between December 1,2019,and December 29,2019,as the data source utilizing the complex network research methodology.It constru... This study selected the Sino-US route data from the top 30 global container liner companies between December 1,2019,and December 29,2019,as the data source utilizing the complex network research methodology.It constructs a Sino-US container shipping network through voyage weighting and analyzes the essential structural characteristics to explore the network’s complex structural fea-tures.The network’s evolution is examined from three perspectives,namely,time,space,and event influence,aiming to comprehens-ively explore the network’s evolution mechanism.The results revealed that:1)the weighted Sino-US container shipping network exhib-its small-world and scale-free properties.Key hub ports in the United States include NEW YORK NY,SAVANNAH GA,LOS ANGELES CA,and OAKLAND CA,whereas SHANGHAI serving as the hub port in China.The geographical distribution of these hub ports is uneven.2)Concerning the evolution of the weighted Sino-US container shipping network,from a temporal perspective,the evolution of the regional structure of the entire Sino-US region and the Inland United States is in a stage of radiative expansion and de-velopment,with a need for further enhancement in competitiveness and development speed.The evolution of the regional structure of southern China and Europe is transitioning from the stage of radiative expansion and development to an advanced equilibrium stage.The shipping development in Northern China,the Western and Eastern United States,and Asia is undergoing significant changes but faces challenges of fierce competition and imbalances.From a spatial perspective,the rationality and effectiveness of the improved weighted Barrat-Barthelemy-Vespignani(BBV)model are confirmed through theoretical derivation.The applicability of the improved evolution model is verified by simulating the evolution of the weighted Sino-US container shipping network.From an event impact per-spective,the Corona Virus Disease 2019(COVID-19)pandemic has not fundamentally affected the spatial pattern of the weighted Sino-US container shipping network but has significantly impacted the network’s connectivity.The network lacks sufficient resilience and stability in emergency situations.3)Based on the analysis of the structural characteristics and evolution of the weighted Sino-US con-tainer shipping network,recommendations for network development are proposed from three aspects:emphasizing the development of hub ports,focusing on the balanced development of the network,and optimizing the layout of Chinese ports. 展开更多
关键词 container shipping network structure characteristics network evolution voyage weighting improved Barrat-Barthelemy-Vespignani(BBV)model
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Ship recognition based on HRRP via multi-scale sparse preserving method
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作者 YANG Xueling ZHANG Gong SONG Hu 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第3期599-608,共10页
In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) ba... In order to extract the richer feature information of ship targets from sea clutter, and address the high dimensional data problem, a method termed as multi-scale fusion kernel sparse preserving projection(MSFKSPP) based on the maximum margin criterion(MMC) is proposed for recognizing the class of ship targets utilizing the high-resolution range profile(HRRP). Multi-scale fusion is introduced to capture the local and detailed information in small-scale features, and the global and contour information in large-scale features, offering help to extract the edge information from sea clutter and further improving the target recognition accuracy. The proposed method can maximally preserve the multi-scale fusion sparse of data and maximize the class separability in the reduced dimensionality by reproducing kernel Hilbert space. Experimental results on the measured radar data show that the proposed method can effectively extract the features of ship target from sea clutter, further reduce the feature dimensionality, and improve target recognition performance. 展开更多
关键词 ship target recognition high-resolution range profile(HRRP) multi-scale fusion kernel sparse preserving projection(MSFKSPP) feature extraction dimensionality reduction
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Improved Scatter Search Algorithm for Multi-skilled Personnel Scheduling of Ship Block Painting
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作者 Guanglei Jiao Zuhua Jiang +1 位作者 Jianmin Niu Wenjuan Yu 《Journal of Harbin Institute of Technology(New Series)》 CAS 2024年第1期1-15,共15页
This paper focuses on the optimization method for multi-skilled painting personnel scheduling.The budget working time analysis is carried out considering the influence of operating area,difficulty of spraying area,mul... This paper focuses on the optimization method for multi-skilled painting personnel scheduling.The budget working time analysis is carried out considering the influence of operating area,difficulty of spraying area,multi-skilled workers,and worker’s efficiency,then a mathematical model is established to minimize the completion time. The constraints of task priority,paint preparation,pump management,and neighbor avoidance in the ship block painting production are considered. Based on this model,an improved scatter search(ISS)algorithm is designed,and the hybrid approximate dynamic programming(ADP)algorithm is used to improve search efficiency. In addition,the two solution combination methods of path-relinking and task sequence combination are used to enhance the search breadth and depth. The numerical experimental results show that ISS has a significant advantage in solving efficiency compared with the solver in small scale instances;Compared with the scatter search algorithm and genetic algorithm,ISS can stably improve the solution quality. Verified by the production example,ISS effectively shortens the total completion time of the production,which is suitable for scheduling problems in the actual painting production of the shipyard. 展开更多
关键词 ship painting personnel scheduling multi⁃skilled workers scatter search task constraints
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Novel method for extraction of ship target with overlaps in SAR image via EM algorithm
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作者 CAO Rui WANG Yong 《Journal of Systems Engineering and Electronics》 SCIE CSCD 2024年第4期874-887,共14页
The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition... The quality of synthetic aperture radar(SAR)image degrades in the case of multiple imaging projection planes(IPPs)and multiple overlapping ship targets,and then the performance of target classification and recognition can be influenced.For addressing this issue,a method for extracting ship targets with overlaps via the expectation maximization(EM)algorithm is pro-posed.First,the scatterers of ship targets are obtained via the target detection technique.Then,the EM algorithm is applied to extract the scatterers of a single ship target with a single IPP.Afterwards,a novel image amplitude estimation approach is pro-posed,with which the radar image of a single target with a sin-gle IPP can be generated.The proposed method can accom-plish IPP selection and targets separation in the image domain,which can improve the image quality and reserve the target information most possibly.Results of simulated and real mea-sured data demonstrate the effectiveness of the proposed method. 展开更多
关键词 expectation maximization(EM)algorithm image processing imaging projection plane(IPP) overlapping ship tar-get synthetic aperture radar(SAR)
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Sending The King Ship, A Tradition Connecting Two Peoples
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作者 Zhu Mengxiao 《China Report ASEAN》 2024年第6期34-36,共3页
“The sky is dark,and it is about to rain,”goes a lyric from China’s coastal Minnan(southern Fujian)region.“The king ship is leaving the bay,papa is going out to sea,and mama is sending the ship off.May it bring us... “The sky is dark,and it is about to rain,”goes a lyric from China’s coastal Minnan(southern Fujian)region.“The king ship is leaving the bay,papa is going out to sea,and mama is sending the ship off.May it bring us wealth,food,and the gods’protection.”The 600-year-old custom is called Ong Chun,Wangchuan,Wangkang,or“Sending the King Ship.” 展开更多
关键词 COASTAL ship REGION
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Decoding RoPax Ship Capsizes and Development of an ISO Ship Safety Standard
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作者 Soonhung Han Robert Latorre 《Engineering(科研)》 2024年第9期225-236,共12页
Marine accidents often result in significant losses of human life, environmental damage, and property destruction. Additionally, ships and offshore plants are large-scale and complex systems, making safety assessments... Marine accidents often result in significant losses of human life, environmental damage, and property destruction. Additionally, ships and offshore plants are large-scale and complex systems, making safety assessments challenging. However, the advent of onboard electronic systems has made it possible to monitor and respond more effectively. These new technologies can enhance safety levels while reducing the workload on crews. In this paper, authors analyze recent accidents involving ships with high structures above the water, such as car carriers or RoPax vessels, and propose preventive safety indicators to help prevent similar accidents from recurring. 展开更多
关键词 ship Accident CAPSIZE RoPax ship Profile Height Regulatory Body ISO Standards
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欧盟AUTOSHIP项目
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作者 王思佳 《中国船检》 2024年第1期70-73,共4页
欧洲水域自主航运倡议项目(Autonomous Shipping Initiative for European Waters,AUTOSHIP项目)由意大利Ciaotech S.r.l.公司牵头,联合康士伯等其他欧洲国家合作伙伴共同完成。该项目通过在不同环境下运营的两艘不同类型船舶上安装和... 欧洲水域自主航运倡议项目(Autonomous Shipping Initiative for European Waters,AUTOSHIP项目)由意大利Ciaotech S.r.l.公司牵头,联合康士伯等其他欧洲国家合作伙伴共同完成。该项目通过在不同环境下运营的两艘不同类型船舶上安装和测试自主航行设施,加速新一代自主航行船舶发展,并为欧盟实现船舶自主航行制定商业化路线图。 展开更多
关键词 ship 欧盟 合作伙伴
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Corporate Social Responsibility and Shipping Supply Chain Risks
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作者 Wen-Chi Lo 《Economics World》 2024年第3期145-155,共11页
This study aims to investigate whether Corporate Social Responsibility(CSR)activities reduce supply chain disruptions by examining the impact of the Suez Canal obstruction on the Ever Given container ship in March 202... This study aims to investigate whether Corporate Social Responsibility(CSR)activities reduce supply chain disruptions by examining the impact of the Suez Canal obstruction on the Ever Given container ship in March 2021.This study conclude that the more responsible companies have higher returns and are less affected by this event than the less responsible companies;the less responsible companies have lower returns.The companies with better CSR have a lower impact on their supply chains when faced with disruptions in the supply chain. 展开更多
关键词 Suez Canal obstruction corporate social responsibility supply chain risks event study Ever Given container ships
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慢性粒细胞性白血病中SHIP1基因的表达变化及机制探讨 被引量:10
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作者 刘小军 罗建民 +5 位作者 杨琳 温树鹏 姚丽 杜行严 杨敬慈 董作仁 《解放军医学杂志》 CAS CSCD 北大核心 2008年第6期701-703,共3页
目的观察SHIP1基因在慢性粒细胞性白血病(CML)患者中的表达变化,并通过特异性小干扰RNA(siRNA)封闭K562细胞株BCR-ABL基因的表达,探讨BCR-ABL基因表达对SHIP1基因的影响。方法应用RQ-PCR方法检测CML患者骨髓、正常人外周血白细胞及白血... 目的观察SHIP1基因在慢性粒细胞性白血病(CML)患者中的表达变化,并通过特异性小干扰RNA(siRNA)封闭K562细胞株BCR-ABL基因的表达,探讨BCR-ABL基因表达对SHIP1基因的影响。方法应用RQ-PCR方法检测CML患者骨髓、正常人外周血白细胞及白血病细胞株K562中SHIP1基因表达的差异。另取K562细胞分为3组:空白对照组(不加任何干扰成分);非特异性siRNA处理组(加非特异性siRNA);特异性siRNA处理组(加入特异性siRNA封闭BCR/ABL融合基因),应用RQ-PCR及Western blot方法分别检测3组中BCR/ABL、SHIP1基因的mRNA及其相应蛋白P210(BCR/ABL编码)、P145(SHIP1基因编码)的表达,并比较各组中表达水平的变化。结果CML患者骨髓白细胞和K562细胞中SHIP1基因的表达明显低于正常人的外周血白细胞。特异性siRNA处理组中BCR-ABL基因mRNA和P210蛋白的表达水平明显下降,SHIP1基因mRNA和P145蛋白表达水平随BCR-ABL表达下降而增加;非特异性siRNA处理组与空白组相比无明显差异。结论CML患者SHIP1基因的表达低于正常人;特异性siRNA能够抑制BCR-ABL基因的表达;BCR-ABL基因能抑制SHIP1基因及其蛋白表达。 展开更多
关键词 白血病 髓样 慢性 BCR/ABL基因 ship1基因 RNA 小分子干扰
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伊马替尼对K562细胞内SHIP基因表达的影响及促凋亡作用 被引量:4
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作者 任金海 罗建民 +4 位作者 杜行严 杨敬慈 姚丽 尚银涛 刘小军 《肿瘤》 CAS CSCD 北大核心 2007年第11期853-856,共4页
目的:观察甲磺酸伊马替尼干预K562细胞后SHIP、caspase-1、caspase-3、caspase-9及bcl-2基因表达的变化,初步探讨伊马替尼促凋亡的作用途径。方法:将不同浓度甲磺酸伊马替尼与K562细胞在体外共培养,于不同时段留取标本,采用实时定量PCR... 目的:观察甲磺酸伊马替尼干预K562细胞后SHIP、caspase-1、caspase-3、caspase-9及bcl-2基因表达的变化,初步探讨伊马替尼促凋亡的作用途径。方法:将不同浓度甲磺酸伊马替尼与K562细胞在体外共培养,于不同时段留取标本,采用实时定量PCR方法检测SHIP基因表达水平的变化,并用半定量逆转录PCR方法检测抗凋亡基因bcl-2及促凋亡基因caspase-1、caspase-3、caspase-9表达水平的变化,用MTT法观察伊替尼对细胞增殖的抑制作用,用膜联蛋白Ⅴ/碘化丙锭(AnnexinⅤ/PI)双标记法分析细胞凋亡。结果:甲磺酸伊马替尼可使K562细胞内SHIP基因表达水平升高,且与剂量和作用时间有关;caspase-9表达水平亦升高并与SHIP基因表达水平有直线相关关系;而caspase-1、caspase-3和bcl-2表达水平无显著变化;伊马替尼可使细胞增殖率降低、凋亡率增加,并可见到明显的形态学变化。结论:甲磺酸伊马替尼可使K562细胞内SHIP及caspase-9基因表达水平升高且可促进细胞凋亡,其促凋亡作用机制可能与上调SHIP和caspase-9基因表达有关。 展开更多
关键词 白血病 粒-单核细胞 慢性 细胞系 肿瘤 基因 ship 基因表达 细胞凋亡
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外源性SHIP基因对K562细胞周期及其相关蛋白表达的影响 被引量:3
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作者 杨琳 罗建民 +5 位作者 王福旭 刘晓军 温树鹏 成志勇 武学文 杨晓阳 《肿瘤》 CAS CSCD 北大核心 2009年第3期201-205,共5页
目的:探讨SHIP基因对人白血病细胞系K562细胞周期的调控作用。方法:用携带野生型SHIP基因及绿色荧光蛋白(green fluorescent protein,GFP)基因的慢病毒及空载体慢病毒转染人白血病K562细胞。FCM检测转染效率、细胞凋亡率及细胞周期变化... 目的:探讨SHIP基因对人白血病细胞系K562细胞周期的调控作用。方法:用携带野生型SHIP基因及绿色荧光蛋白(green fluorescent protein,GFP)基因的慢病毒及空载体慢病毒转染人白血病K562细胞。FCM检测转染效率、细胞凋亡率及细胞周期变化,实时荧光定量PCR(real-time fluorescent quantitative PCR,RFQ-PCR)检测SHIPmRNA水平的变化,Western印迹法检测SHIP、cyclinD1、p21WAF1/CIPI、p27KIP1蛋白表达水平的变化。结果:与转染空载体组和未转染组相比,转染野生型SHIP基因的K562细胞增殖减慢,凋亡率明显上升(P<0.05);细胞周期显示,G0/G1期延长,G1期前出现亚二倍体的凋亡峰,S期和G2/M期比例降低;cyclin D1表达降低,p21WAF1/CIPI和p27KIP1表达增加。结论:转染野生型SHIP基因可使细胞周期阻滞在G0/G1期,抑制白血病细胞增殖,促进白血病细胞凋亡。 展开更多
关键词 白血病 实验性 转染 细胞周期 细胞周期蛋白类 K562细胞 基因 ship
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SHIP2在放射线处理后喉癌Hep-2细胞中的增殖、凋亡及细胞周期中作用 被引量:3
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作者 梁慧玲 何晓琴 +4 位作者 甘园园 周宇杰 熊琳 唐甜 徐细明 《中国医药导报》 CAS 2017年第36期4-8,20,共6页
目的研究放疗后SHIP2在喉癌Hep-2细胞中的表达情况及与Hep-2细胞增殖、凋亡及细胞周期之间的对应关系。方法取对数生长期的喉癌Hep-2细胞,分为实验组(2、4、8 Gy X线)与对照组(0 Gy X线)两组,各组给予不同剂量X线处理后12、24、36、48 h... 目的研究放疗后SHIP2在喉癌Hep-2细胞中的表达情况及与Hep-2细胞增殖、凋亡及细胞周期之间的对应关系。方法取对数生长期的喉癌Hep-2细胞,分为实验组(2、4、8 Gy X线)与对照组(0 Gy X线)两组,各组给予不同剂量X线处理后12、24、36、48 h,运用CCK-8检测Hep-2细胞增殖活力。根据细胞活力检测结果,确定射线处理后最适宜的时间点作为后续实验检测的具体时间点。实验组与对照组给予对应处理后特定时间,运用Annexin V-FITC/PI双染流氏细胞术检测细胞凋亡,PI单染流式细胞术检测细胞周期,RT-q PCR检测SHIP2m RNA相对表达量,Western-blot检测SHIP2蛋白的相对表达量。结果与对照组比较,实验组Hep-2细胞活性降低(P<0.05),且随放射剂量递增,细胞增殖活力降低,处理后48 h检测结果最为显著。实验组各组Hep-2细胞凋亡数较对照组均明显增加(均P<0.01)。实验组中给予4 Gy、8 Gy X线照射处理的Hep-2细胞G2/M期阻滞与对照组相比显著增加(均P<0.01)。实验组Hep-2细胞中SHIP2的m RNA及蛋白相对表达量较对照组均有所降低(均P<0.01)。结论放射线处理后SHIP2的下调表达可能是X线抑制Hep-2细胞增殖、促进其细胞凋亡和G2/M期阻滞的重要途径。 展开更多
关键词 X线 ship2 增殖 凋亡 细胞周期
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多个零售商竞争下的Drop-shipping供应链协调 被引量:4
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作者 李根道 熊中楷 李薇 《科技管理研究》 CSSCI 北大核心 2006年第10期249-252,共4页
D rop-sh ipp ing是互联网上广泛采用的一种商业模式。Netessine(2004)对这种模式的供应链进行了研究,分析了多周期供应商和零售商之间的相互作用。本文扩展了Netessine的研究,分析了一对多的D rop-sh ip-p ing供应链,零售商之间存在促... D rop-sh ipp ing是互联网上广泛采用的一种商业模式。Netessine(2004)对这种模式的供应链进行了研究,分析了多周期供应商和零售商之间的相互作用。本文扩展了Netessine的研究,分析了一对多的D rop-sh ip-p ing供应链,零售商之间存在促销竞争,讨论了Nash均衡的存在性和唯一性。研究发现在一对多的情况下,Netessine设计的合同的局限性。数值分析发现保持一定数量的零售商对供应商有好外,但零售商太多也会存在弊端。 展开更多
关键词 Drop—shipping 供应链 竞争
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