Effect of Cytokinins on the Micropropagation of Carob(Ceratonia siliqua L.)through Shoot Tip Culture

In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l);high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l.

满天星试管正常苗与玻璃苗叶片解剖结构的比较

在本文中利用解剖学方法,对满天星试管正常苗和玻璃苗叶片解剖结构进行了观察比较,结果表明:二者的表皮均由单层细胞构成,气孔突出,叶肉组织无细胞的分化,是等面叶,且叶肉中通气组织发达。与试管正常苗相比,玻璃苗的叶片厚,气孔突出明显,表皮细胞、叶肉细胞体积膨大,有的叶肉细胞的细胞壁发育不全,在某些区域出现空洞,壁的形态呈现不完整的现象。玻璃苗叶片的维管组织呈现退化的状态。