Liver fibrosis markers in alcoholic liver disease

Alcohol is one of the main factors of liver damage. The evaluation of the degree of liver fibrosis is of great value for therapeutic decision making in patients with alcoholic liver disease(ALD). Staging of liver fibrosis is essential to define prognosis and management of the disease. Liver biopsy is a gold standard as it has high sensitivity and specificity in fibrosis diagnostics. Taking into account the limitations of liver biopsy, there is an exigency to introduce non-invasive serum markers for fibrosis that would be able to replace liver biopsy. Ideal serum markers should be specific for the liver, easy to perform and independent to inflammation and fibrosis in other organs. Serum markers of hepatic fibrosis are divided into direct and indirect. Indirect markers reflect alterations in hepatic function, direct markers reflect extracellular matrix turnover. These markers should correlate with dynamic changes in fibrogenesis and fibrosis resolution. The assessment of the degree of liver fibrosis in alcoholic liver disease has diagnostic and prognostic implications, therefore noninvasive assessment of fibrosis remains important. There are only a few studies evaluating the diagnostic and prognostic values of noninvasive biomarkers of fibrosis in patients with ALD. Several noninvasive laboratory tests have beenused to assess liver fibrosis in patients with alcoholic liver disease, including the hyaluronic acid, FibroTest, FibrometerA, Hepascore, Forns and APRI indexes, FIB4, an algorithm combining Prothrombin index(PI), α-2 macroglobulin and hyaluronic acid. Among these tests, Fibrotest, FibrometerA and Hepascore demonstrated excellent diagnostic accuracy in identifying advanced fibrosis and cirrhosis, and additionally, Fibrotest was independently associated with survival. Therefore, the use of biomarkers may reduce the need for liver biopsy and permit an earlier treatment of alcoholic patients.

Biomarkers for detection of alcohol consumption in liver transplantation

Alcoholic liver disease is an established, yet controversial, indication for liver transplantation. Although an abstinence period of up to 6 mo prior to transplantation is mandatory, alcohol relapse after transplantation is a common event. In case of recurrence of heavy drinking, graft survival is significantly impaired. Guidelines on detection and surveillance of alcohol consumption in this patient cohort are lacking. This review summarizes the challenge of patient selection as well as the current knowledge on established and novel alcohol biomarkers with special focus on liver transplant candidates and recipients.

Advances in the prediction of biological markers of alcohol dependence relapse

Alcohol dependence is a chronic and re-lapsing disease that causes mental and organ damage due to long-term heavy drinking,which has serious adverse effccts on individuals and socicty.Previous studics have shown that even after inpatient treatment,the relapse rate of alcohol-dependent patients can be as high as 60%-70%within 3 months after discharge.Currently,the mecha-nisms associated with relapse to drinking in alcohol-dependent patients are still unclear.And most of the studies reported in the past have been about the relationship between psychosocial factors and relapse to drinking,while few studies have examined the relationship between bi-ological indicators and relapse to drinking.Therefore,in this paper,we summarize the recent literature to explore the biological markers related to alcohol dependence relapse and discuss the progress of research on relapse pre-diction,in order to provide reference and help to reduce the relapse rate and improve the quality of life of patients.

Serum immunoglobulin A concentration is a reliable biomarker for liver fibrosis in non-alcoholic fatty liver disease

AIM:To evaluate the diagnostic accuracy of serum Immunoglobulin A(IgA)for differentiating early stage nonalcoholic fatty liver disease(NAFLD)from nonalcoholic steatohepatitis(NASH).METHODS:All cases had fatty liver change confirmed by ultrasound and aminotransferases of at least twice the normal level.Clinical and biochemical data,including serum IgA,were obtained from 50 histologically proven NAFLD cases and 54 healthy controls.Fasting whole blood samples were obtained from the study population.Immunoturbidimetric methods were used to measure the IgA levels.All NAFLD cases were hospitalized for liver biopsy.Liver specimens were examined for steatosis,steatohepatitis and fibrosis within hepatocytes.Patients were categorized into two groups:NASH and non-NASH.Variables were compared within cases(NASH vs non-NASH)and controls.Cut-off values of serum IgA were evaluated using analysis of receiver operating characteristic(ROC curves).Associations between the variables were tested using calculations of correlation coefficients.Statistical significances were assigned to P values<0.05.RESULTS:The extent of liver fibrosis correlated positively with IgA levels.Subjects with no fibrosis in their liver biopsies had a lower IgA level(301.5±91.2 mg/dL)than subjects with any degree of fibrosis(388.8±140.8 mg/dL),(P=0.01).IgA levels were higher in NASH cases,and its value was significantly higher for higher degrees of fibrosis.Patients with perisinusoidal or pericellular fibrosis had significantly higher levels of IgA(403.5±133.9 mg/dL,418.2±129.5 mg/dL)compared to those without it(301.8±94.9 mg/dL,297.7±91.5 mg/dL),respectively.No significant correlation was found between steatosis grade and serum IgA levels.Based on ROC analysis,the best predictive IgA cutoff value for detecting liver fibrosis was 360mg/dL(61%sensitivity,81%specificity).CONCLUSION:The serum IgA level is useful to evaluate the severity of liver fibrosis and can be used serially for evaluation and follow-up of NAFLD cases.

Is increased red cell distribution width an indicating marker of nonalcoholic steatohepatitis and fibrotic stage?

AIM:To evaluate the red cell distribution width(RDW)as an indicator of the presence of non-alcoholic steatohepatitis(NASH)and its association with fibrotic scores.METHODS:A retrospective study was carried out that included sixty-two biopsy proven NASH,32 simple steatosis patients and 30 healthy controls.The correlation between the clinical and histopathological features of NASH patients and RDW values was evaluated.Liver fibrosis scores were measured using a 0 to 4 point scale and were divided in to two groups;fibrosis scores0-1 were termed mild and fibrosis scores 2-4 were termed advanced fibrosis.RDW values were compared between NASH,simple steatosis and healthy controls.Univariate and multivariate analyses were performed to evaluate the independent predicting factors for the presence of liver fibrosis caused by NASH.RESULTS:Patients with NASH had higher RDW values compared with simple steatosis and healthy control groups[14.28%±0.25%vs 13.37%±0.12%,12.96%±0.14%(P<0.01),respectively].Patients with advanced fibrosis had higher RDW values than the mild fibrosis group(15.86%±0.4%vs 13.63%±0.67%,P<0.01,respectively).RDW also correlated with fibrotic scores(r=0.579 andP<0.01).The variables that were significant in the univariate analysis were evaluated in multivariate logistic regression analysis,and RDW was an independent predicting factor of NASH(OR=1.75,95%CI:1.129-2.711,P<0.05).CONCLUSION:RDW a new non-invasive marker that can be used to demonstrate the presence of NASH and indicate advanced fibrotic scores.

Detection of alcoholic liver disease

INTRODUCTIONAlcohol has been used in society over centuries and all over the world for its mood-lifting properties and taste. It is probably ,however ,the commonest drug of abuse world-wide and unfortunately causes considerable morbidity, mortality and social disruption .In 1990 the cost tl the USA was more than $ 100 billion and 100 000 lives.The relationship between alcohol and mankind is well documented from the earliest tines .

Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales

BACKGROUND Seeking potentially novel blood markers of liver fibrosis and steatosis is constantly of crucial importance.Despite a growing number of studies in this field of hepatology,a certain role of hematological indices in the course of liver disorders has not been fully elucidated,yet.AIM To evaluate a diagnostic accuracy of neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR)and mean platelet volume-to-platelet-ratio(MPR)in the course of alcoholic liver cirrhosis(ALC)and nonalcoholic fatty liver disease(NAFLD).METHODS One hundred forty-two patients with ALC,92 with NAFLD and 68 persons in control group were enrolled in the study.Hematological indices(NLR,PLR and MPR),indirect and direct markers of liver fibrosis(aspartate transaminase to alkaline transaminase ratio,aspartate transaminase to platelet ratio index,fibrosis-4,gamma-glutamyl transpeptidase to platelet ratio,procollagen Ⅰ carboxyterminal propeptide,procollagen Ⅲ aminoterminal propeptide,transforming growth factor-α,platelet-derived growth factor AB,laminin)were measured in each person.Model for end-stage liver disease(MELD)score in ALC group and NAFLD fibrosis score together with BARD score were calculated in NAFLD patients.Receiver operating characteristic(ROC)curves and area under the curve(AUC)values were applied to assess the sensitivity and specificity of examined markers and to evaluate proposed cut-offs of measured indices in the course of ALC and NAFLD.RESULTS MPR and NLR values in ALC patients were significantly higher in comparison to control group;PLR level was significantly lower.MPR and PLR correlated with assessed indirect and direct markers of liver fibrosis.MPR,NLR and PLR correlated with MELD score.NLR level in NAFLD patients was significantly higher in comparison to controls.MPR correlated with indirect markers of liver fibrosis and NAFLD fibrosis score.AUC values and proposed cut-offs for NLR,PLR and MPR in ALC patients were:0.821(>2.227),0.675(<70.445)and 0.929(>0.048),respectively.AUC values and proposed cut-offs for NLR,PLR and MPR in NAFLD group were:0.725(>2.034),0.528(>97.101)and 0.547(>0.038),respectively.CONCLUSION Hematological markers are inseparably connected with serological indices of liver fibrosis in ALC and NAFLD patients.MPR and NLR turned out to be the most powerful parameters in ALC patients.

Non-invasive diagnosis of alcoholic liver disease

Alcoholic liver disease(ALD)is the most common liver disease in the Western world.For many reasons,it isunderestimated and underdiagnosed.An early diagnosis is absolutely essential since it(1)helps to identify patients at genetic risk for ALD;(2)can trigger efficient abstinence namely in non-addicted patients;and(3)initiate screening programs to prevent life-threateningcomplications such as bleeding from varices,spontaneous bacterial peritonitis or hepatocellular cancer.The two major end points of ALD are alcoholic liver cirrhosis and the rare and clinically-defined alcoholic hepatitis(AH).The prediction and early diagnosis of both entities is still insufficiently solved and usually relies on acombination of laboratory,clinical and imaging findings.It is not widely conceived that conventional screeningtools for ALD such as ultrasound imaging or routine laboratory testing can easily overlook ca.40%of manifest alcoholic liver cirrhosis.Non-invasive methods such as transient elastography(Fibroscan),acoustic radiation force impulse imaging or shear wave elastography have significantly improved the early diagnosis of alcoholiccirrhosis.Present algorithms allow either the exclusion or the exact definition of advanced fibrosis stages in ca.95%of patients.The correct interpretation of liver stiffness requires a timely abdominal ultrasound and actual transaminase levels.Other non-invasive methods such as controlled attenuation parameter,serum levels of M30 or M65,susceptometry or breath tests are under current evaluation to assess the degree of steatosis,apoptosis and iron overload in these patients.Liver biopsy still remains an important option to rule out comorbidities and to confirm the prognosis namely for patients with AH.

Diagnostic challenges in alcohol use disorder and alcoholic liver disease

Alcohol use disorders represent a heterogeneous spectrum of clinical manifestations that have been defined by the Diagnostic and Statistical Manual of Mental Disorders-5. Excessive alcohol intake can lead to damage of various organs, including the liver. Alcoholic liver disease includes different injuries ranging from steatosis to cirrhosis and implicates a diagnostic assessment of the liver disease and of its possible complications. There is growing interest in the possible different tools for assessing previous alcohol consumption and for establishing the severity of liver injury, especially by noninvasive methods.

多靶标联合分析在空勤人员非酒精性脂肪肝病筛查中的价值

目的探讨体检数据与非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的相关性,建立基于多靶标联合分析的NAFLD高效诊断模型。方法回顾性分析2021-01~12月作者单位疗养员的临床资料,通过训练集(80%样本)构建诊断模型并利用验证集(20%样本)评价诊断效果。联合Logistic回归和受试者工作特征(receiver operating characteristic,ROC)曲线筛选并评价不同组合的诊断效能。结果Logistic回归分析显示,体质量指数(body mass index,BMI)、丙氨酸氨基转移酶(alanine transaminase,ALT)、高密度脂蛋白(high-density lipoprotein,HDL)、尿酸(uric acid,UA)和血小板(platelet,PLT)与NAFLD密切相关。ROC曲线分析发现,联合BMI、ALT、HDL、UA和PLT的多靶标诊断模型显示出色的诊断价值,训练集中的曲线下面积(area under the curve,AUC)达0.981(95%CI:0.971~0.991),验证集为0.951(95%CI:0.931~0.972)。结论多靶标联合诊断模型对NAFLD具有重要价值,能满足体检人群和空勤疗养员NAFLD的筛检需求。

乙醇对男性生殖系统功能的影响

目的 研究长期饮酒对男性生殖系统功能的损伤。方法 选择 4 5名长期饮酒者和 9名慢性酒精中毒者为实验组 ,2 0名不饮酒者为对照组 ,检测精液质量 ,血清中睾酮 (T)、雌二醇 (E2 )、黄体生成素 (LH)和促卵泡素 (FSH)的水平及血清、精浆和精子中乳酸脱氢酶 (LDH)、乳酸脱氢酶同工酶 -x(LDH -x)、葡萄糖 - 6磷酸脱氢酶 (G - 6PD)、葡萄糖苷醛酸酶 (β -G)活力。结果 饮酒达 10年以上 ,饮酒量为每日 10 0～ 15 0g 38℃白酒 ,每月饮 2 0次以上 ,其精子数、活动精子率、精子中的LDH -x活性均明显低于正常对照组 (均P <0 0 5 ) ,饮酒量为每日 15 0～ 2 5 0g 38℃白酒 ,每月饮 2 0次以上 ,血清中T下降 ,E2 、FSH、LH均升高 (均P <0 0 5或P <0 0 1) ,精浆和精子中的LDH、LDH -x、G - 6PD和 β -G活性均明显低于对照组 (均P <0 0 5 ) ,尤以LDH -x敏感。 结论 长期酗酒对男性生精功能、睾丸标志酶及生殖内分泌激素水平产生严重影响。

急性酒精中毒患者心肌生化标记物测定及其临床意义

目的探讨急性酒精中毒患者心肌生化标记物的变化及其临床意义。方法检测80例急性酒精中毒患者心肌生化标记物,并比较分析其心脏事件发生率。同时,选取16名健康者作为常规对照。结果急性酒精中毒患者心肌生化标记物及心脏事件发生率显著高于健康对照组。结论急性酒精中毒可引起心肌生化标记物的改变,造成心肌一过性损害。

血液中一种新的“濒亡标志物”(醇脱氢酶同工酶)的鉴定

目的 鉴定血清乳酸脱氢酶 (LD)同工酶检测中出现的第 6条带“LD6”的蛋白质性质。方法 用琼脂糖凝胶电泳分离、提纯“LD6” ,聚丙烯酰胺凝胶电泳检测“LD6”相对分子质量 ,免疫转移印迹法鉴定人血清中“LD6”抗原性。结果 测定人血清LD同工酶时出现的第 6条带“LD6”为醇脱氢酶 (alcoholdehydrogenase ,AD)同工酶 ,相对分子质量为 80 0 0 0 ,用western免疫固定转印法鉴定核实。正常人肝组织匀浆、肝癌组织匀浆作琼脂糖凝胶电泳分离、提纯“LD6” ,聚丙烯酰胺凝胶电泳检测 ,表明肝组织匀浆、肝癌组织匀浆的“LD6”与血清“LD6”为同一物质。 1 0 0例患者有 37例血清含有AD ,其中 34例AD活性≥LD总活性7.9% ,病人 1周内死亡 ,死亡率达 91 .9%。结论 正常人血清内不易检测出AD活性 ,而正常肝组织内有强的AD Ⅱ活性。当肝脏受到严重损害时 ,AD从肝细胞内逸出 ,释放入血 ,导致血清AD活性明显升高。血清中AD活性上升可能是一种新的“濒亡标志物”。

乙醇灌胃对大鼠胃黏膜损伤修复相关信号分子的探讨

目的:为了探讨乙醇灌胃法对SD大鼠和其自身启动的修复机制及相关的信号分子的影响。方法:雄性SD大鼠20只,适应性喂养1周后,禁食24h,取10只用无水乙醇按1mg/kg灌胃造模型,其余10只灌以相同剂量的生理盐水。24h后采用链霉蛋白酶消化法分离胃黏膜细胞,用表面加强激光解析电离飞行时间质谱技术(SELDI-TOF-MS)及WCX2蛋白芯片获得胃黏膜损伤大鼠和对照组大鼠胃黏膜细胞的蛋白质指纹图谱,通过对比分析两组的差异的蛋白质荷比峰,从而寻找出乙醇致胃黏膜损伤的特异相关分子。结果:对照组与模型组比较共有4个蛋白质有显著性差异(P<0.05),其中两个蛋白质/荷比峰明显升高,分别是3727.126、8586.021,两个蛋白质/荷比峰明显降低,分别是10777.71、18708.49。

组织多肽抗原联合ProGRP、CEA、NSE、SCC、CYFRA21-1在肺癌诊治中的价值

目的评估组织多肽抗原(TPA)联合Pro GRP、CEA、NSE、SCC、CYFRA21-1在肺癌诊断与疗效监测中的应用价值。方法用化学发光法和电化学发光法检测238例肺癌患者、25例肺部良性疾病患者及65名健康对照者血清中的TPA、Pro GRP、NSE、SCC、CYFRA21-1和CEA水平,并对33例肺癌患者进行随访检测。同时用SPSS19.0统计软件及接受器工作性能曲线(ROC)分析,评价肿瘤标志物的临床应用价值。结果肺癌患者血清TPA水平(中位数为130.45 U/L)明显高于肺部良性疾病患者(中位数为82.21 U/L)和健康对照组(中位数为70.96 U/L)(P=0.000,0.002)。根据ROC曲线分析,TPA检测肺癌的临界值为130 U/L,敏感度为50%,特异性为88.9%,相比于其他肺癌标志物(Pro GRP、NSE、SCC、CYFRA21-1、CEA),敏感度较高,特异性稍低。肺癌患者血清TPA水平及阳性率随着肿瘤分期的升高而升高(P均<0.05)。TPA水平与疗效也密切相关,临床治疗有效时TPA下降,而病情恶化或出现转移时则升高。各种组合检测中,以六项组合诊断肺癌的敏感度和有效性最高。结论 TPA联合Pro GRP、CEA、NSE、SCC、CYFRA21-1测定在肺癌的诊断、疗效及监测复发转移中,具有一定的临床价值。

不同剂量白酒对大鼠心肌影响的研究

目的 观察不同剂量白酒对大鼠心肌损伤标志物及心肌组织的影响并探讨其机制,为临床防治乙醇性心肌病提供理论基础和实验依据.方法 140只2～3月龄健康SD大鼠按随机排列表随机分为对照组及不同剂量白酒灌胃模型组[A组0.8 ml/（kg·d）、B组1.6 ml/（kg·d）、C组2.4 ml/（kg·d）、D组3.2 ml/（kg·d）、E组4.0 ml/（kg·d）、F组4.8 ml/（kg·d）],每组20只.对照组每日给予10 ml/（kg·d）生理盐水灌胃,其余模型组分别给予相应浓度白酒灌胃,每周称重1次,灌胃6次,连续灌胃16周.16周后每只大鼠禁食12 h取颈总静脉血检测肌酸激酶同工酶（CK-MB）、肌钙蛋白T（cTnT）;处死大鼠取心肌组织进行病理学检查,同时取大鼠心肌组织用TUNEL法检测细胞凋亡相关蛋白半胱氨酸天冬氨酸蛋白酶（Caspase）-3的表达水平.结果 A、B组与对照组相比,CK-MB、cTnT水平差异均无统计学意义（均P＞0.05）.C、D、E、F组与对照组及A、B组相比,CK-MB、cTnT水平明显升高,差异均有统计学意义（均P＜0.01）.对照组及A～F组大鼠的Caspase-3水平（吸光度值）分别为（93.76±14.76）、（92.49±15.39）、（93.19±13.36）、（94.99±14.33）、（196.67±25.16）、（239.86 ±20.99）、（246.66±23.16）.D、E、F组分别与对照组、A、B、C组相比,差异均有统计学意义（均P＜0.05）.A、B、C组与对照组相比,Caspase-3水平均差异无统计学意义（P＞0.05）.结论 适量[0.8～1.6 ml/（kg·d）]饮酒不会引起心肌损伤标志物升高及细胞病理学改变,但过量乙醇摄入可导致心肌酶谱变化、Caspase-3表达增加、心肌细胞凋亡和坏死.

凯西莱联用复方丹参注射液治疗酒精性肝病临床观察

目的探讨凯西莱联用复方丹参注射液治疗酒精性肝病(ALD)的临床效果。方法将160例ALD患者随机分为治疗组和对照组,每组80例,对患者治疗前后进行临床评估和检测肝功能、AFP、肝纤维化等指标变化情况。结果治疗组治疗28天后临床症状和体征除肝区疼痛明显改善外(P<0.05),其余症状与对照组比较差异无显著性(P>0.05);两组治疗后肝功能、肝纤维化四项及AFP均有明显改善,但治疗组A/G、AST以及肝纤维化四项指标改善程度均优于对照组(P<0.01)。无明显毒副作用。结论凯西莱能有效改善ALD患者的临床症状、体征及肝功能、AFP,联合应用复方丹参注射液疗效更佳,尤其是抗肝纤维化效果更为明显,且毒副作用少,具有临床应用价值。

基于一测多评法对肠胃散中8种物质的质量控制

目的建立一测多评法同时测定肠胃散中棕矢车菊素、异泽兰黄素、柠檬苦素、吴茱萸碱、吴茱萸次碱、肉桂醇、肉桂酸和桂皮醛的含量。方法色谱柱:Waters Symmetry C18柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)-0.1%磷酸溶液(B),梯度洗脱,流速:1.0 ml/min;检测波长分别为345 nm(检测棕矢车菊素和异泽兰黄素)、215 nm(柠檬苦素、吴茱萸碱和吴茱萸次碱)和275 nm(肉桂醇、肉桂酸和桂皮醛)。以吴茱萸碱为内参物,分别建立其他7种成分的相对校正因子,并用相对校正因子计算含量,实现一测多评;同时与外标法测定结果进行对比,验证一测多评法的准确性和可行性。结果棕矢车菊素、异泽兰黄素、柠檬苦素、吴茱萸碱、吴茱萸次碱、肉桂醇、肉桂酸和桂皮醛分别在0.98~19.60、2.67~53.40、4.06~81.20、1.98~39.60、2.69~53.80、0.56~11.20、1.49~29.80和8.77~175.40μg/ml范围内线性关系良好(r≥0.9992),平均加样回收率(RSD)分别为98.77%(0.96%)、99.38%(1.01%)、100.02%(0.83%)、97.80%(1.40%)、98.91%(1.18%)、96.99%(1.13%)、98.09%(1.24%)和99.10%(0.67%),一测多评法计算结果与外标法实测值无明显差异。结论所建立的方法简便、准确,可用于肠胃散的质量评价。

蜜蜂样本处理对形态遗传标记的影响

为了明确常用的蜜蜂样本伸吻处理方法和酒精保存方法,是否会对蜜蜂的形态遗传标记测定产生影响。我们分别进行了开水处理样本和酒精长期浸泡处理对形态遗传标记影响的对比实验。开水处理和酒精浸泡1年的各项形态标记与未经处理的标本差异均不显著(P>0.05)。本研究证明,为了使蜜蜂的吻完整伸出,准确测定,可以采用开水处理蜜蜂标本,用于形态测定的中华蜜蜂标本可以用酒精浸泡至少保存1年。

葡萄醇酰基转移酶编码基因遗传变异研究

【目的】开发适用于葡萄香气表型鉴定的分子标记,为葡萄分子辅助育种提供理论依据。【方法】采用固相微萃取结合气相质谱法对45个葡萄品种进行香气组分和含量测定,并分别采用Sanger测序和扩增子测序的方法对葡萄醇酰基转移酶编码基因(VvAAT)结构变异和SNP变异进行分析。【结果】45个葡萄品种中共发现65种香气组分,可以分成酯类、醇类、萜类和醛类4种类型,其中酯类香气含量表现出显著的品种差异性,‘巨峰’等20个葡萄品种酯类香气含量丰富,而‘87-1’等25个葡萄品种检测不到酯类香气;VvAAT共发现了5种结构变异类型,类型I、II、IV和V由于过早终止密码子或片段插入变异,不能正确翻译,仅类型III可以正常翻译,根据其氨基酸序列系统进化树分析结果,又可以分成III.1和III.2两种类型,结合香气测定数据,III.1为功能型,其余均为非功能型;扩增子测序及生信分析发现了8个位于外显子区域的SNP位点(T32C、A69T、G436C、A1247G、A1818T、G1929T、A1959G和C1975G),均导致了编码氨基酸的突变,可以准确区分酯香型品种和非酯香型品种,准确率为97.8%。【结论】葡萄VvAAT位点存在丰富的遗传变异,包括基因结构变异和SNP变异;位于编码区的8个SNP位点能够准确判定葡萄酯类香气表型,可以应用于葡萄分子辅助育种。