Effect of Shuanghuanglian Oral Solution on Liver Function in a Mouse Model of Non-alcoholic Steatohepatitis

[Objectives]To observe the effect of Shuanghuanglian oral solution on liver function in BABL/cJ mice in non-alcoholic steatohepatitis(NASH)model.[Methods]The BABL/cJ mice were randomly divided into three groups:a control group,a model group,and an experimental group.The experimental group was administered with 10%Shuanghuanglian oral solution at a dose of 0.1 mL/(10 g·d),while the control group and experimental group received an equivalent dosage of normal saline.All three groups were treated for a period of 28 d.The liver function of the mice in each group was examined after the treatment.[Results]The body mass,liver index,triacylglycerol(TG),total cholesterol(TC),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)levels were all significantly reduced compared to the model group(P<0.05).[Conclusions]Shuanghuanglian oral solution has a beneficial effect on liver function in BABL/cJ mice.

Study on the Effect of Shuanghuanglian Powder Needle Combined with Cefoperazone and Sulbactam Sodium on Pseudomonas aeruginosa in Vitro

Objective: To explore the antibacterial activity of combined use of Shuanghuanglian and cefoperazone sulbactam sodium on resistant strains of Pseudomonas aeruginosa. Methods: The Pseudomonas aeruginosa strains which were sensitive and resistant to cefoperazone sulbactam sodium were selected to prepare different test bacterial solutions respectively;The experimental liquid of Shuanghuanglian and Cefoperazone Sulbactam Sodium were prepared separately and set as different test groups and control groups;The Drug Sensitivity Tests of Shuanghuanglian and cefoperazone sulbactam sodium at different concentration gradients which were used alone or used in combination were carried out for different strains with sensitivity and resistance, And use standard entry as a reference control. Result: The results of drug sensitivity test of Shuanghuanglian combined with Cefoperazone-Sulbactam sodium against the resistant strains of Pseudomonas aeruginosa were compared with the results of drug sensitivity test of the two separately used, and the difference was statistically significant (P 〈 0.05) [The drug sensitivity test results of Shuanghuanglian and cefoperazone sulbactam sodium to Pseudomonas aeruginosa resistant strains were statistically significant compared with the drug sensitivity test results of Shuanghuanglian and Cefoperazone Sulbactam Sodium used separately (P 〈 0.05)];There was a dependence between strains and concentration in the effect of the combination of the two drugs. Conclusion: The combination of Shuanghuanglian and cefoperazone sulbactam sodium has synergistic antibacterial or bactericidal effect on Pseudomonas aeruginosa resistant strains. .

双黄连口服液质量一致性评价研究

目的:探索建立双黄连口服液(SHLO)质量一致性评价方法,并对常见市售8个厂家的产品进行质量分级。方法:建立SHLO中6个指标成分的高效液相色谱含量测定方法,从同厂家不同批次、不同厂家角度分析样品含量均一性;用3个质量一致性参数[批内一致性差异(PA)、批间一致性差异(PB)、指纹图谱相似率(PC)]表征不同厂家产品质量一致性水平;运用主成分分析(PCA)模型提取一致性区分因子(P),实现对8个厂家样品质量一致性分级。结果:建立的高效液相色谱含量测定方法简便且方法学验证合格;40批样品中绿原酸、黄芩苷、连翘苷的质量浓度分别为0.66~1.25、12.71~21.89、0.39~0.66 mg·mL^(–1),均符合《中华人民共和国药典》(以下简称《中国药典》)2020年版对该制剂的限度要求,非《中国药典》2020年版指标成分新绿原酸、连翘酯苷A、汉黄芩苷的质量浓度分别为0.75~1.44、0.07~0.94、0.001 3~1.930 0 mg·mL^(–1),且同厂家样品均一性较好、不同厂家样品存在一定差异;8个厂家样品PA为1.2%~7.7%、PB为16.6%~39.1%、PC为99.3%~99.9%,依据P可将8个生产厂家样品分为3类,其中厂家F、R产品的一致性较好。结论:建立了简便的SHLO多成分定量方法,结合3个一致性评价参数和PCA模型可对不同生产厂家样品质量进行区分,数据结果可为各生产厂家质量标准提升提供参考。

复方双黄连制剂对单核巨噬细胞(RAW264.7)Fc/C3b受体活性和分泌功能的影响

【目的】探究复方双黄连制剂对巨噬细胞吞噬能力和分泌功能的影响,为复方双黄连的深度开发及畜禽用药的合理选择提供科学依据。【方法】将金银花、黄芩、连翘、穿心莲干燥粉碎后分别制备浸膏,按照比例制备双黄连口服液(金银花∶黄芩∶连翘=1∶1∶2)、复方双黄连口服液(金银花∶黄芩∶连翘∶穿心莲=1∶1∶2∶2)及穿心莲口服液,调节pH为7.0,生药浓度为1 g/mL。3种药物作用于小鼠单核巨噬细胞RAW264.7,采用CCK-8法检测细胞活力,确定3种药物安全浓度,将药物最大安全浓度以二倍稀释法稀释为3个浓度;采用脂多糖(LPS)和氢化考的松琥珀酸钠(HCSS)作用于RAW264.7细胞模拟机体炎症和免疫抑制状态,3种药物作用于这2种状态的细胞和正常细胞,采用YC、EA玫瑰花环法及中性红吞噬法检测巨噬细胞Fc/C3b受体活性和吞噬能力,采用ELISA法检测巨噬细胞分泌能力。【结果】复方双黄连、双黄连、穿心莲的安全浓度分别为0.625~2.5、3.125~12.5和0.625~2.5 mg/mL。不同浓度复方双黄连、双黄连、穿心莲作用于巨噬细胞,细胞吞噬能力均显著高于空白对照组(P<0.05)。复方双黄连可降低LPS巨噬细胞RAW264.7活跃的吞噬能力,增强HCSS巨噬细胞RAW264.7吞噬能力;不同浓度复方双黄连可使活化的巨噬细胞Fc/C3b受体活性下降,低下的Fc/C3b受体活性增强;不同浓度复方双黄连具有促进巨噬细胞分泌一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)及溶菌酶(LZM)的作用,抑制LPS巨噬细胞NO、TNF-α、IFN-γ及白细胞介素-6(IL-6)的分泌。【结论】复方双黄连可通过活化巨噬细胞Fc/C3b受体活性以增强机体免疫力和抗炎能力,同时对已活化的巨噬细胞分泌功能起到抑制作用,以减少由于免疫功能亢进造成的机体损伤。研究结果为中兽药复方双黄连的深度开发提供了参考依据。

双黄连氧气驱动雾化吸入联合NCPAP治疗对MP合并呼吸衰竭患儿的影响

目的探讨采取双黄连氧气驱动雾化吸入联合经鼻持续气道正压通气(NCPAP)治疗儿童重症支原体肺炎(MP)合并呼吸衰竭的效果及对血清瘦蛋白(Leptin)、白细胞介素⁃17(IL⁃17)及白细胞介素⁃33(IL⁃33)水平的影响。方法选取82例患儿并随机分成观察组42例与对照组40例,对照组采取常规吸氧、止咳及NCPAP治疗,观察组则是在对照组的基础上加用双黄连氧气驱动雾化吸入治疗,比较2组治疗效果。结果观察组患儿各症状消退时间明显比对照组短(P<0.05);治疗后2组血氧饱和度(SaO_(2))、血氧分压(PaO_(2))较治疗前提高,二氧化碳分压(PaCO_(2))较治疗前降低,而观察组指标变化幅度较对照组显著(P<0.05);治疗后2组血清Leptin、IL⁃17、IL⁃33均较治疗前降低,观察组各指标显著低于对照组(P<0.05)。结论双黄连氧气驱动雾化吸入联合NCPAP治疗儿童重症MP合并呼吸衰竭,可显著改善患儿血气功能指标,降低血清炎症因子水平,促进患儿症状的改善。

双黄连注射剂中黄芩素致敏家兔后血清中特异性抗体的测定

目的利用酶联免疫吸附测定法(ELISA)法检测双黄连注射剂中黄芩素致敏家兔后血清中特异性抗体的效价。方法将正常家兔血清与双黄连注射剂中疑似小分子半抗原物质黄芩素在体外一起孵育从而制备所需完全抗原,随后,利用这些完全抗原免疫家兔以期获得特异性抗体。运用ELISA法检测所得抗血清的抗体效价。通过间接竞争ELISA法,进一步测定血清特异性并绘制相关浓度抑制曲线。此外,采用兔免疫球蛋白E(IgE)ELISA试剂盒检测各组家兔抗血清中相对应的IgE抗体含量。结果与阴性对照组比较黄芩素的抗体效价(OD)值是其2倍,表明其具有潜在的致敏性。通过整理数据,绘制抑制率曲线得到回归方程I=0.138lgC+0.402,R^(2)=0.959,显示出良好的拟合度。半数抑制浓度IC_(50)=5.178 mg·mL^(-1)。此外,本实验结果明显表明外源性IgE型抗体已经在家兔体内产生,从而进一步证实了黄芩素的致敏性。结论在本实验所模拟家兔致敏全过程结果中表明黄芩素具有明显的致敏性。这一发现对于双黄连注射剂的安全使用具有重要的指导意义,也为相关药物的研发和生产提供了有价值的参考。

双黄连口服液对H9亚型禽流感的预防和治疗效果

为了评价双黄连口服液对H9亚型禽流感的预防和治疗效果,分别在SPF鸡感染H9亚型禽流感病毒前和感染后口服双黄连口服液,病毒感染后3、5、7、10 d采集喉头和泄殖腔棉拭子,通过实时荧光定量RT-PCR检测排毒率,并在感染后5、7 d剖检观察组织病变,进行病理组织学观察。结果显示,双黄连口服液可降低H9亚型禽流感病毒感染鸡的排毒率,缩短排毒周期,治疗组和预防治疗组病毒感染后10 d排毒率为0,极显著低于病毒感染组排毒率6/10(60%)(P<0.01);治疗组和预防治疗组SPF鸡在感染后5 d和7 d喉头、气管黏膜、肺脏的组织损伤较小;且与治疗组相比,预防治疗组排毒率更低,喉头、气管、肺脏组织病理损伤更轻微。结果表明,双黄连口服液不仅可降低感染鸡的排毒率,缩短鸡体排毒时间。针对呼吸道病原,双黄连口服液预防治疗效果优于感染后治疗效果。该研究为双黄连口服液更好地应用于临床呼吸道疫病的防控提供了新的策略。

双黄连颗粒联合阿奇霉素治疗小儿支原体肺炎的临床效果

目的 探讨双黄连颗粒联合阿奇霉素治疗小儿支原体肺炎的临床效果。方法 选取2020年1月至2022年12月于三原县医院就诊的70例支原体肺炎患儿作为研究对象,按照随机数字表法分为对照组(35例)和观察组(35例)。对照组单用阿奇霉素,观察组在对照组基础上联用双黄连颗粒。比较两组炎症因子水平及临床症状评分。结果 治疗后,两组C反应蛋白、白细胞介素-17、降钙素原和肿瘤坏死因子-α水平均低于治疗前,且观察组低于对照组(P<0.05)。治疗后,两组咳嗽、食欲不振、喘憋、发热评分均低于治疗前,且观察组低于对照组(P <0.05)。结论 双黄连颗粒联合阿奇霉素治疗小儿支原体肺炎的临床效果较好,可有效降低炎症因子水平,减轻患儿临床症状,值得推广。

阿奇霉素序贯疗法联合双黄连口服液治疗儿童肺炎支原体肺炎的效果分析

目的:分析阿奇霉素序贯疗法联合双黄连口服液治疗儿童肺炎支原体肺炎(MPP)的效果。方法:选取2022年7月—2023年6月联勤保障部队第九九一医院收治的MPP患儿96例作为研究对象,按照随机数字表法分为对照组和研究组,各48例。对照组采用阿奇霉素序贯疗法,研究组在对照组基础上联合双黄连口服液治疗。比较两组治疗效果、症状消失时间、炎性因子水平以及不良反应发生情况。结果:研究组治疗总有效率高于对照组,差异有统计学意义(P=0.025)。研究组咳嗽、发热、肺部啰音消失时间均早于对照组,差异有统计学意义(P<0.001)。治疗前,两组白细胞介素-6(IL-6)、超敏C反应蛋白(hs-CRP)水平比较,差异无统计学意义(P>0.05);治疗后,两组IL-6、hs-CRP水平降低,研究组低于对照组,差异有统计学意义(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:阿奇霉素序贯疗法联合双黄连口服液治疗儿童MPP效果理想,可促进症状消退,抑制炎性反应,且未增加不良反应发生风险。

Rapid and Sensitive Assay of the Main Components in Shuanghuanglian Preparations by UPLC-TOF-MS

1 Introduction As already known, complex prescription has been the outstanding feature of traditional Chinese medicine（TCM）. This fact therefore brings complicated formula with multicomponents from multimaterals, which produces some difficulties to detect the components and to control the quality. Shuanghuanglian preparations（SHLs）, including oral liquid, injection powder, capsule, and tablet, belong to a family of refined traditional Chinese patent medicine and a combined herbal remedy comprised of three herbs：

Inhibitory effects of Shuanghuanglian injection on nuclear factor-kappa B expression in mice with viral encephalitis in a time-and dose-dependent manner

Previous studies have confirmed that the anti-virus effects of Shuanghuanglian injection may be associated with nuclear factor-kappa B activity. This study observed nuclear factor-kappa B expression in mice with viral encephalitis, and showed significant decreases in nuclear factor-kappa B protein and mRNA levels following Shuanghuanglian injection. The inhibitory effect was more significant with prolonged intervention duration and increased treatment dose. These findings verify that Shuanghuanglian injection plays a therapeutic role in viral encephalitis by reducing expression of nuclear factor-kappa B in a time- and dose-dependent manner.

Outlining the keyword co-occurrence trends in Shuanghuanglian injection research: A bibliometric study using CiteSpace Ⅲ

Objective:To explore the evolvement and new trends in the use of Shuanghuanglian injection(SHLI).Methods:China National Knowledge Infrastructure(CNKI),PubMed,and Embase were extensively searched using the search terms“Shuanghuanglian injection”and“Shuanghuanglian fenzhen”to retrieve articles relevant to SHLI(1992e2020).Retrieved articles were further investigated by two authors to exclude those unrelated to SHLI.The bibliographical references of the included articles were exported as raw data and then treated using the CiteSpace tool to visualize the mapping of the SHLI research domain.Essential clusters and highly frequent keywords were quantified for further analysis.The clusters were automatically labeled by the algorithm of tf*idf for objective analysis.Basic bibliometric features,including article types and yearly trend in article numbers were also determined and discussed.Results:The modules of the keywords of interest presented clear boundaries with a high modularity score(Q=0.73).High-confidence clusters were identified,including bioactivity fingerprint(S=0.99),equal pupils(S=0.91),drug preparation department(S=0.87),difficulty in respiration(S=0.85),peristalsis(0.88),and Danshen powder injection(S=0.94).The characteristic keywords in terms of frequency and burstiness were Shuanghuanglian powder for injection(F=235,B=5.22),SHLI(F=112,B=11.39),and adverse drug reactions(ADRs;F=104,B=7.35).Conclusion:In the field of SHLI study,there are five major topic categories:bioactivity fingerprint;ADR mechanism and cause detection;proper preparation;clinical evidence accumulation;and efficacy in diseases with no effective treatment and combination usage.The trend for using modern methodologies from a science-based perspective to study SHLI will continue to exist.The causes of multi-factorial ADRs may be an important topic for future studies.

Shuanghuanglian injection downregulates nuclear factor-kappa B expression in mice with viral encephalitis

A mouse model of viral encephalitis was induced by intracranial injection of a Coxsackie virus B3 suspension. Quantitative real-time reverse transcription-PCR and western blot assay were applied to detect mRNA and protein expression of intelectin-2 and nuclear factor-kappa B in the viral encephalitis and control groups. Nuclear factor-kappa B and intelectin-2 mRNA and protein expression were significantly increased in mice with viral encephalitis. After intraperitoneal injection of Shuanghuanglian at a dose of 1.5 mg/kg for 5 successive days, intelectin-2 and nuclear factor-kappa B protein and mRNA expression were significantly decreased. To elucidate the relationship between intelectin-2 and nuclear factor-kappa B, mice with viral encephalitis were administered an intracerebral injection of 107 pfu recombinant lentivirus expressing intelectin shRNA. Both protein and mRNA levels of intelectin and nuclear factor-kappa B in brain tissue of mice were significantly decreased. Experimental findings suggest that Shuanghuanglian injection may downregulate nuclear factor-kappa B production via suppression of intelectin production, thus inhibiting inflammation associated with viral encephalitis.

Effects of Shuanghuanglian Propolis Oral Liquid on Growth Performance and Intestinal Microflora of Broilers

[ Objective] The paper was to investigate the effects of adding different levels of Shuanghuanglian propelis oral liquid in drinking water on growth performance and intestinal microflora of broilers. [ Method] Ninety one-day-old broilers were randomly divided into three groups （group I, group 1I and group III）： group I was the control group, and groups II and III were added with 0.5 and 1.0 mL/L Shuanghuanglian prepolis oral liquid in drinking water, respec- tively. [ Result] Compared with broilers in group I, the average daily gains of broilers in group II and group III increased significantly （ P 〈 0.05 ） ; the feed gain ratio of broilers in group III was significandy lower than that in group I （ P 〈 0.05 ） ; the number of intestinal LactobaciUus spp. in group II and group III in- creased significantly （ P 〈 0.05 ） ; the number of Salmonella spp. and Escherichia coli in group lII decreased significantly （ P 〈 0.05 ） ; the number of Bgrdobacterium spp. increased significantly （ P 〈 0.05 ）. [ Conclusion] Adding 0.5 and 1.0 mL/L Shuanghuanglian propolis oral liquid in drinking water could improve growth performance and intestinal micreflora of broilers, and the dose of 1.0 mL/L is recommended in clinical application.

Effect of Shuanghuanglian Propolis Oral Liquid on Immune Function of Broilers

The effects on immune function of broilers was studied by adding different doses of Shuanghuanglian propolis oral liquid in drinking water, to provide a theoretical basis for application and promotion of Shuanghuanglian propolis oral liquid. Ninety one-day-old broilers were randomly divided into three groups ： group I was designed as control; groups Ⅱ and Ⅲ were added with 0.5 and 1.0 mL/L Shuanghuanglian propolis oral liquid in drinking water, respectively. Compared with group I, all tested indicators in groups Ⅱ and Ⅲ were increased at varying degrees as follows ： bursa of fabricius index increased by 5.74% （P 〈 0.05 ） and 7.66% （P 〈 0.05 ） ; thymus index increased by 1.47% （P 〉 0.05 ） and 2.06% （P 〈 0.05 ） ; spleen index increased by 5.88% （P 〉 0.05 ） and 7.84% （ P 〈 0.05 ） ; Newcastle disease virus （NDV） antibody titer increased by 11.99% （P 〈 0.05 ） and 16.94% （P 〈 0.05 ） ; IL-2 content increased by 2.71% （P 〉 0.05） and 4.52% （P 〈 0.05 ） ; IL -6 content increased by 4.04% （P 〉 0.05 ） and 7.26% （ P 〈 0.05 ）, respectively. The results showed that adding 0.5 and 1.0 mL/L Shuanghuangllan propolis oral liquid in drinking water could improve the immune function of broiler and immune effect of ND vaccine.

发酵双黄连药渣对断奶仔猪生长性能、血清生化指标、免疫指标及肠道菌群的影响

试验旨在研究发酵双黄连药渣对断奶仔猪生长性能、血清生化指标、免疫指标及肠道菌群的影响。选取21日龄健康“杜×长×大”三元杂交断奶仔猪96头,随机分为4组,每组4个重复,每个重复6头仔猪。A组(对照组)断奶仔猪饲喂基础日粮,B组和C组分别在基础日粮中添加2%发酵药渣(植物乳杆菌LP-1522发酵双黄连药渣)和2%未发酵药渣;D组在基础日粮中添加乳酸菌菌剂(108 CFU/L)。预试期7 d,正式试验期28 d。结果显示,与对照组相比,B组断奶仔猪的平均日采食量、平均日增重分别显著提高10.37%和16.48%(P<0.05),腹泻率和料重比显著降低(P<0.05);B组断奶仔猪血清总蛋白含量显著升高7.34%(P<0.05),免疫球蛋白G(IgG)、免疫球蛋白A(IgA)含量显著升高(P<0.05),尿素氮含量显著降低(P<0.05)。饲喂发酵双黄连药渣后,断奶仔猪肠道中乳酸杆菌的相对丰度明显升高,大肠埃希氏菌-志贺菌及链球菌等致病性细菌的相对丰度明显降低。研究表明,发酵双黄连药渣可以提高断奶仔猪的生长性能,抑制肠道病原菌,降低腹泻率,提高免疫性能。

Efficacy and safety of atomized inhalation for Shuanghuanglian on chronic pharyngitis:a Meta-analysis of the randomized controlled trials

Objective: As a Chinese drugs preparation to clear heat and remove toxicity, Shuanghuanglian (SHL) has been widely used, but the clinical efficacy and safety of SHL on chronic pharyngitis remain unclear, especially the application of atomized inhalation of SHL need to be verified. The aim of this study was to evaluate its clinical efficacy and safety for chronic pharyngitis. Methods: From the Cochrane Library, Pubmed, EMbase, Wanfang Datebase, CNKI (China National Knowledge Infrastructure), VIP (Chinese Scientifc Journals Database), CBM (Chinese Biomedicine Database), We got the qualified randomized controlled trials(RCTs) and adopted RevMan5.3 software and the risk of bias tool according to the Cochrane to perform a metaanalysis. Results: Ten RCTs, a total of 950 cases (involving 472 cases in SHL group and 478 in control group) were included. The results showed that the clinical efficacy in SHL group was superior to control group [Chi2=5.61, 95%CI (4.29, 11.43), P<0.05], and four literatures reported no obvious adverse reactions in SHL group. Conclusion: Application of atomized inhalation of SHL may have a potential advantage in treating chronic pharyngitis. However, due to the limitation of the quality and sample size of those studies, the accuracy of the result should be treated with caution, higher standardized researches are required to justify the conclusion.

Component Analysis of Shuanghuanglian Freeze-dried Powder and Its Effects on Human Hepatocyte Function when Combined with Cefradine

[Objectives]To detect the contents of components in Shuanghuanglian freeze-dried powder and to explore the effects of Shuanghuanglian freeze-dried powder and its components on human hepatocytes(HL-7702)alone or in combination with cefradine.[Methods]High performance liquid chromatography(HPLC)was used to detect the contents of baicalin,wogonin,chlorogenic acid and forsythin,the main components of Shuanghuanglian freeze-dried powder.HL-7702 cells were cultured with Shuanghuanglian freeze-dried powder and the main components of Shuanghuanglian freeze-dried powder alone or in combination with cefradine.Enzyme linked immunosorbent assay(ELISA)was used to detect the contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the cell supernatant after culture,and HPLC was used to detect the expression level of adenosine diphosphate(ADP)and adenosine triphosphate(ATP);agarose gel electrophoresis was used to detect the expression of cyclooxygenase 2(COX-2)and heme oxygenase-1(HO-1)in HL-7702 cells.[Results]In Shuanghuanglian freeze-dried powder for injection,the content of baicalin was the highest,and the content of wogonin was the lowest.Compared with the control group,the expressions of AST and ALT in human hepatocytes(HL-7702)in high-dose baicalin group,forsythin group and wogonin group decreased(P<0.05,P<0.01),while the expression of ALT in chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)increased(P<0.05,P<0.01),and the expression of AST had no significant difference(P>0.05);the results in the low-dose group were similar to those in the high-dose group.Compared with the control group,ATP expression in chlorogenic acid group,chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)in the high-dose group decreased(P<0.05,P<0.01),and ADP expression was not significantly different(P>0.05);in the low-dose group,the expression of ATP and ADP increased in baicalin group(P<0.05),but decreased in wogonin group,baicalin+cefradine group(0.046 mg/mL)and wogonin group+cefradine group(0.046 mg/mL)(P<0.05,P<0.01).Compared with the control group,the expressions of COX-2 and HO-1 in HL-7702 cells in the cefradine group showed no significant difference(P>0.05).The expression of HO-1 and COX-2 in the different dose groups of Shuanghuanglian and the group combined with cefradine increased,and the difference was significant(P<0.05,P<0.01).[Conclusions]The components of Shuanghuanglian freeze-dried powder for injection had effects on hepatocytes,of which baicalin had a significant effect,and the effect of cefradine on hepatocytes was increased when used in combination with cefradine.

Study on pharmacodynamic mechanism of compound Shuanghuanglian in prevention and treatment of pneumonia based on network pharmacology and association analysis

Background:Platform for prediction and analysis of Chinese medicine against coronavirus disease 2019(TCMAntiCOVID-19 V1.0)was used to explore the active ingredients,key targets and mechanisms of the compound coronavirus disease 2019(COVID-19),It will provide a reference for the clinical application of this prescription as a broad-spectrum agent in the prevention and treatment of pneumonia.It will also win valuable time for finding symptomatic Chinese medicine prescriptions and vaccine research and development.Methods:Set Banxia Tianma Baizhu decoction as the negative control.Qingfei Paidu decoction as the positive control,and use TCMAntiCOVID-19 V1.0 platform to predict the potential efficacy of compound Shuanghuanglian.We used the quantitative evaluation algorithm of multi-target drugs for the network disturbance of disease,by comparing the changes of network topological characteristics before and after drug intervention,and using the disturbance rate to evaluate the drug’s dryness for disease prediction.Using batman-TCM,the credibility card value of the target sets 20,then the target of traditional Chinese medicine composition is predicted,the heterogeneous network of traditional Chinese medicine composition-drug target-disease target is established.The interaction of related network is realized by JavaScript Visualization is realized,and cycloscape is edited.The average connectivity,the average shortest path length,the centrality of connectivity and the centrality of network compactness are calculated by using R’s iGraph package,and nulldistribution is used as the overall distribution to correct the disturbance rate of drugs to the real network,so as to evaluate the stability of network topology and explore its mechanism.Results:The key targets of COVID-19 were Aif1,Ccl2,Cdc20,Cxcl13,Fcer1g,Ido1,Ifng,Il10,Il1rnIl6,Ncf4,Ptger4,Spi1,Tnf,Xcl1;after the intervention,the average connectivity,the average shortest path length,the network connectivity centrality and the network tightness centrality were(2.31e+1),(2.41e+0),(6.21e−1),(1.68e−2)respectively;the total scores of network stability evaluation of drug intervention diseases were 18.29,12.59 and 19.10 respectively.Conclusion:Compound Shuanghuanglian can effectively break the stability of the disease network of COVID-19,and the overall effect of prevention and treatment of COVID-19 is close to that of the positive control Qingfei Paidu decoction,which is significantly better than that of the negative control Banxia Tianma Baizhu decoction.That is because compound Shuanghuanglian mainly acts on Aif1,Ccl2,Cdc20,Cxcl13,Fcer1g and other key targets.Compound Shuanghuanglian plays important role of inhibiting the inflammatory response of patients with COVID-19 and alleviating lung injury,so as to achieve the purpose of treating COVID-19.At the same time,in the initial stage of COVID-19 and other sudden infectious pneumoniausing compound Shuanghuanglian can win valuable time before finding symptomatic Chinese medicine prescription and vaccine research.

响应面法优化双黄连口服液醇沉物乳酸菌发酵工艺

为研究双黄连口服液制剂醇沉物的乳酸菌液态发酵工艺,首先确定双黄连口服液制剂醇沉物添加量,再以活菌数作为指标,通过单因素试验分别研究接种量、发酵时间和发酵温度的影响,并采用响应面法优选最佳发酵条件,最后进行平行放大试验验证。结果:含20%的醇沉物、接种量4%、发酵温度32.7℃、发酵时间26 h时活菌数可达3.03×10^(9)CFU/mL,为最佳发酵条件。平行放大验证试验表明,该发酵工艺可用于制备双黄连口服液醇沉物乳酸菌液态发酵制剂。