Objective To determine whether the anti-inflammatory properties of Shuxiong Tablet (SXT) and the effective components group of SXT (ECGS) are equivalent and to assess the formulary rationality. Methods ECGS consisted ...Objective To determine whether the anti-inflammatory properties of Shuxiong Tablet (SXT) and the effective components group of SXT (ECGS) are equivalent and to assess the formulary rationality. Methods ECGS consisted of Panax notoginsen saponion (PNS), hydroxysafflor yellow A, and ferulic acid plus volatile oil of Ligusticum chuanxiong, which was based on the active ingredients and their ratios in SXT. We compared the anti-inflammatory actions of ECGS and SXT using the xylene-induced edema model and the carrageenan-induced edema model, as well as the analgesic activity of them using the acetic acid-induced writhing model. Moreover, cultured macrophages were incubated with media containing serum isolated from SXT-, ECGS-, or every component of ECGS-treated rats, to compare the depress effects on lipopolysaccharide (LPS)-stimulated NO production and inducible nitric oxide synthase (iNOS) expression. Results ECGS and SXT had equivalent anti-inflammatory actions and analgesic effects at an equipotent dosage in a dose-dependent manner. The drug-containing media could inhibit the LPS-stimulated NO production and iNOS expression in cultured macrophages. A 2 × 2 × 2 ANOVA revealed that three effective components could produce synergistic effect on the inhibition of NO production, and PNS was the capital component. Conclusion ECGS and SXT display an equivalent anti-inflammatory effect, and the formula follows traditional Chinese medicine compatibility principle, which shows obvious formulary rationality.展开更多
目的建立测定舒胸片和舒胸微丸中羟基红花黄色素A含量的高效液相色谱法。方法固定相:Phenomenex Luna C18柱(4.6mm×250mm,5μm);流动相:乙腈-0.05%磷酸(15∶85);流速:1.0ml/min;检测波长:403nm;柱温:25℃。结果羟基红花黄色素A在2...目的建立测定舒胸片和舒胸微丸中羟基红花黄色素A含量的高效液相色谱法。方法固定相:Phenomenex Luna C18柱(4.6mm×250mm,5μm);流动相:乙腈-0.05%磷酸(15∶85);流速:1.0ml/min;检测波长:403nm;柱温:25℃。结果羟基红花黄色素A在2.65～26.50μg·ml-1范围内线性关系良好(r=0.9999)。舒胸片和舒胸微丸的平均加样回收率分别为99.4%和100.5%,RSD分别为1.6%和2.0%。结论该方法简便快速,适用于舒胸片和舒胸微丸中羟基红花黄色素A的含量测定。展开更多
基金National Natural Science Foundation of China (30430790)
文摘Objective To determine whether the anti-inflammatory properties of Shuxiong Tablet (SXT) and the effective components group of SXT (ECGS) are equivalent and to assess the formulary rationality. Methods ECGS consisted of Panax notoginsen saponion (PNS), hydroxysafflor yellow A, and ferulic acid plus volatile oil of Ligusticum chuanxiong, which was based on the active ingredients and their ratios in SXT. We compared the anti-inflammatory actions of ECGS and SXT using the xylene-induced edema model and the carrageenan-induced edema model, as well as the analgesic activity of them using the acetic acid-induced writhing model. Moreover, cultured macrophages were incubated with media containing serum isolated from SXT-, ECGS-, or every component of ECGS-treated rats, to compare the depress effects on lipopolysaccharide (LPS)-stimulated NO production and inducible nitric oxide synthase (iNOS) expression. Results ECGS and SXT had equivalent anti-inflammatory actions and analgesic effects at an equipotent dosage in a dose-dependent manner. The drug-containing media could inhibit the LPS-stimulated NO production and iNOS expression in cultured macrophages. A 2 × 2 × 2 ANOVA revealed that three effective components could produce synergistic effect on the inhibition of NO production, and PNS was the capital component. Conclusion ECGS and SXT display an equivalent anti-inflammatory effect, and the formula follows traditional Chinese medicine compatibility principle, which shows obvious formulary rationality.
文摘目的建立测定舒胸片和舒胸微丸中羟基红花黄色素A含量的高效液相色谱法。方法固定相:Phenomenex Luna C18柱(4.6mm×250mm,5μm);流动相:乙腈-0.05%磷酸(15∶85);流速:1.0ml/min;检测波长:403nm;柱温:25℃。结果羟基红花黄色素A在2.65～26.50μg·ml-1范围内线性关系良好(r=0.9999)。舒胸片和舒胸微丸的平均加样回收率分别为99.4%和100.5%,RSD分别为1.6%和2.0%。结论该方法简便快速,适用于舒胸片和舒胸微丸中羟基红花黄色素A的含量测定。
