Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-...Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside (2), beta -D-glucopyranosyl-ent-15,16-dihydroxypimar-8(14)-en-19-oiclate (3), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-gluco-pyranoside (4), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside-15,16-acetonide (5) by 1D and 2D NMR techniques.展开更多
Siegesbeckia pubescens（SP） has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unc...Siegesbeckia pubescens（SP） has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear. The present study aimed to examine the effects of the essential oil of SP（SPEO） on the proliferation of hepatocellular carcinoma cells and the possible mechanisms. The growth inhibition of Hep G2 cells was analyzed by MTT assay. Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect cell apoptosis and cell cycle. The expressions of the target proteins were detected by Western blotting. The results showed that SPEO obviously inhibited the proliferation of Hep G2 cells in a dose-dependent manner. SPEO activated a series of apoptotic proteins in Hep G2 cells, increasing expression levels of Bax, caspase-3 and caspase-9, and decreasing the bcl-2 expression level. SPEO displayed promising anti-hepatocellular carcinoma activities in vitro, partly by inducing apoptosis in Hep G2 cells through activating the mitochondrial pathway.展开更多
One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8（14）-en-15-al （1） together with four known diterpenoids kirenol （2）, ent-2-oxo-15,16,19-trihydroxypimar-8（14）-ene （3）, darutigenol （4） and darutosi...One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8（14）-en-15-al （1） together with four known diterpenoids kirenol （2）, ent-2-oxo-15,16,19-trihydroxypimar-8（14）-ene （3）, darutigenol （4） and darutoside （5） were isolated from the ethanol extract of Siegesbeckia pubescens. The planar structures and relative configurations of these compounds were elucidated by comprehensive spectroscopic analysis.展开更多
Objective:To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens(AFSP).Methods:Adjuvant arthritis(AA)model of rat was produced to observe the effect of AFSP on lymphocyte pr...Objective:To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens(AFSP).Methods:Adjuvant arthritis(AA)model of rat was produced to observe the effect of AFSP on lymphocyte proliferation,interleukin-1 and-2(IL-1,-2)activity,pathologic section of ankle joint,and analgesic effect,in model rat.Results:AFSP could reduce the inflammatory pathologic response of ankle joint.It has good analgesic effect,the analgesic rate being 65%.AFSP could also strengthen T-lymphocyte proliferation,promote IL-2 activity and inhibit IL-1 activity.Compared with the control group,the difference was significant(P<0.01).Conclusion:By means of regulating the immune function of organism,AFSP could improve the local pathologic response to antagonize against rheumatism,therefore,it is an effective anti-rheumatism herbal medicine.展开更多
基金Projec twas supported by the Applied Basic Research Foundation of Yunnan Province (97C089M).
文摘Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside (2), beta -D-glucopyranosyl-ent-15,16-dihydroxypimar-8(14)-en-19-oiclate (3), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-gluco-pyranoside (4), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside-15,16-acetonide (5) by 1D and 2D NMR techniques.
基金financially supported by grants from the Major Research Project of Education Department of Hubei Province of China(No.D20131103)National Natural Science Foundation of China(No.81573561)the Introduction Project of Hangzhou Medical College,Zhejiang Province,China(No.2015B08)
文摘Siegesbeckia pubescens（SP） has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear. The present study aimed to examine the effects of the essential oil of SP（SPEO） on the proliferation of hepatocellular carcinoma cells and the possible mechanisms. The growth inhibition of Hep G2 cells was analyzed by MTT assay. Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect cell apoptosis and cell cycle. The expressions of the target proteins were detected by Western blotting. The results showed that SPEO obviously inhibited the proliferation of Hep G2 cells in a dose-dependent manner. SPEO activated a series of apoptotic proteins in Hep G2 cells, increasing expression levels of Bax, caspase-3 and caspase-9, and decreasing the bcl-2 expression level. SPEO displayed promising anti-hepatocellular carcinoma activities in vitro, partly by inducing apoptosis in Hep G2 cells through activating the mitochondrial pathway.
基金supported by the National Natural Science Foundation of China(Grant No.30973628)the National Science and Technology Project of China(Grant No.2012ZX09103201-022)
文摘One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8（14）-en-15-al （1） together with four known diterpenoids kirenol （2）, ent-2-oxo-15,16,19-trihydroxypimar-8（14）-ene （3）, darutigenol （4） and darutoside （5） were isolated from the ethanol extract of Siegesbeckia pubescens. The planar structures and relative configurations of these compounds were elucidated by comprehensive spectroscopic analysis.
文摘Objective:To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens(AFSP).Methods:Adjuvant arthritis(AA)model of rat was produced to observe the effect of AFSP on lymphocyte proliferation,interleukin-1 and-2(IL-1,-2)activity,pathologic section of ankle joint,and analgesic effect,in model rat.Results:AFSP could reduce the inflammatory pathologic response of ankle joint.It has good analgesic effect,the analgesic rate being 65%.AFSP could also strengthen T-lymphocyte proliferation,promote IL-2 activity and inhibit IL-1 activity.Compared with the control group,the difference was significant(P<0.01).Conclusion:By means of regulating the immune function of organism,AFSP could improve the local pathologic response to antagonize against rheumatism,therefore,it is an effective anti-rheumatism herbal medicine.
