Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into ...Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into the normal group, SQD model group (model group), SQD+ still water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats each group;Grip-Strength Meter was used to measure limb grip strength;transmission electron microscope was employed to observe the ultrastructural changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear numbers, cross-section area (CSA) and then their ratios (the MND sizes) were calculated, immunofluorescence assay was chosen to test the expressions of paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). Results: Compared with those in the normal group, limb grip strength was decreased, sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes were reduced, but the Pax7+ cell numbers were increased, significantly, in the model and SW groups;Compared with those in the model and SW groups, limb grip strength was increased, sarcomeres were basically normal, the myonuclear number and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both increased, significantly, in the CM group. Conclusion: Sijunzi decoction might increase the myonuclear number by activating the MSCs to treat limb weakness in SQD.展开更多
Objective:To investigate a previously uncharacterized function of Sijunzi Decoction(SJZD)in inhibition of gastric cancer stem cells(GCSCs).Methods:MKN74 and MKN45,two CD44 positive gastric cancer cell lines with stem ...Objective:To investigate a previously uncharacterized function of Sijunzi Decoction(SJZD)in inhibition of gastric cancer stem cells(GCSCs).Methods:MKN74 and MKN45,two CD44 positive gastric cancer cell lines with stem cell properties were used.The cells were divided into 2 groups.Treatment group was treated with SJZD(1-5 mg/mL)for indicated time(48 h-14 days).The control group was treated with equal volume of phosphate buffered saline.Cell Counting Assay Kit-8 were used to measure cell viability.Spheroid colony formation and GCSCs marker expression were performed to determine GCSCs stemness.Cell fractionation and chromatin immunoprecipitation assays were used to assess the distribution and DNA-binding activity of β-catenin after SJZD treatment,respectively.Results:SJZD treatment repressed cell growth and induced apoptosis in MKN74 and MKN45 cell lines(P<0.05).Moreover,SJZD dramatically inhibited formation of spheroid colony and expression of GCSC markers in GC cells(P<0.05).Mechanistically,SJZD reduced nuclear accumulation and DNA binding activity ofβ-catenin(P<0.05),the key regulator for maintaining CSC stemness.Conclusion:SJZD inhibits GCSCs by attenuating the transcriptional activity of β-catenin.展开更多
文摘Objective: To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods: 40 male Sprague-Dawley rats were randomly divided into the normal group, SQD model group (model group), SQD+ still water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats each group;Grip-Strength Meter was used to measure limb grip strength;transmission electron microscope was employed to observe the ultrastructural changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear numbers, cross-section area (CSA) and then their ratios (the MND sizes) were calculated, immunofluorescence assay was chosen to test the expressions of paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). Results: Compared with those in the normal group, limb grip strength was decreased, sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes were reduced, but the Pax7+ cell numbers were increased, significantly, in the model and SW groups;Compared with those in the model and SW groups, limb grip strength was increased, sarcomeres were basically normal, the myonuclear number and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both increased, significantly, in the CM group. Conclusion: Sijunzi decoction might increase the myonuclear number by activating the MSCs to treat limb weakness in SQD.
基金Supported by National Natural Science Foundation of China(No.81703916)Natural Science Foundation of Hunan Province(No.2018JJ6042)。
文摘Objective:To investigate a previously uncharacterized function of Sijunzi Decoction(SJZD)in inhibition of gastric cancer stem cells(GCSCs).Methods:MKN74 and MKN45,two CD44 positive gastric cancer cell lines with stem cell properties were used.The cells were divided into 2 groups.Treatment group was treated with SJZD(1-5 mg/mL)for indicated time(48 h-14 days).The control group was treated with equal volume of phosphate buffered saline.Cell Counting Assay Kit-8 were used to measure cell viability.Spheroid colony formation and GCSCs marker expression were performed to determine GCSCs stemness.Cell fractionation and chromatin immunoprecipitation assays were used to assess the distribution and DNA-binding activity of β-catenin after SJZD treatment,respectively.Results:SJZD treatment repressed cell growth and induced apoptosis in MKN74 and MKN45 cell lines(P<0.05).Moreover,SJZD dramatically inhibited formation of spheroid colony and expression of GCSC markers in GC cells(P<0.05).Mechanistically,SJZD reduced nuclear accumulation and DNA binding activity ofβ-catenin(P<0.05),the key regulator for maintaining CSC stemness.Conclusion:SJZD inhibits GCSCs by attenuating the transcriptional activity of β-catenin.
