The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of suc...The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of successful cross breeding in silkworm. Protein samples from silk gland, hemolymph, and midgut were separated by 2-dimensional gel electrophoresis （2-DE）. In the three tissues the matched protein spots between Jinqiu and its cross parents were approximately 70% with approximately 30% specific protein spots. In the matched protein spots, 9-24% was differentially expressed representing up- and down-regulated expression. These specific protein spots might be either the newly appeared, which were produced from the genic interaction of cross parents＇ genes in cross breeding, or posttranscriptionally modified, which were produced from the different modifications on the same original proteins. These results indicate that it is important for a new successful breed, by cross breeding, relying on the actions of some newly produced functional proteins from genic interaction, in addition to marshaling excellent genes of cross parents.展开更多
The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excell...The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excellent silkworm variety Jingsong×Haoyue were taken as recurrent parents,using the natural green cocoon variety G 9202 as donor parent.After other excellent parents were introduced to cross with these different lines,four new breeding materials were selected by recurrent backcross breeding.The characters of these breeding materials showed not only natural green cocoon but also excellent comprehensive traits.A naturally colored cocoon quaternary hybrid with excellent practical characters was selected based on multiple hybrids.It is suitable for spring rearing in Yangtze valley which includes Hunan Province.It was approved by Hunan Provincial Crop Variety Approval Committee and designated Xiangcailu No.1 in 2016.展开更多
家蚕血液型脓病是由家蚕核型多角体病毒(Bombyx mori nuclear polyhedrosis virus,BmNPV)引起的一种病害,症状表现为体壁紧张发亮,高节肿胀;行动狂躁,常爬行于蚕座四周;最后体壁破裂,流出脓汁而死,这会严重影响家蚕的生长和发育。为了...家蚕血液型脓病是由家蚕核型多角体病毒(Bombyx mori nuclear polyhedrosis virus,BmNPV)引起的一种病害,症状表现为体壁紧张发亮,高节肿胀;行动狂躁,常爬行于蚕座四周;最后体壁破裂,流出脓汁而死,这会严重影响家蚕的生长和发育。为了有效防治家蚕血液型脓病,研究人员通过选育抗血液型脓病家蚕品种,取得了一定的进展。本文综述了BmNPV的特点、传播途径和致病机理等方面,并重点介绍了抗血液型脓病家蚕品种的选育方法和进展。通过梳理近几年来相关文献,总结现有抗血液型脓病家蚕品种的选育策略和主要特点,并对未来的研究方向进行了展望。本文对于家蚕血液型脓病的防治以及抗血液型脓病家蚕品种的选育具有一定的参考价值。展开更多
基金supported by the National Basic Re-search Program of China (973 Program,2005CB121003)the National High-Tech Research and Development Program of China (863 Program,2006AA10A118)the Doctoral Fund of Ministry of Education of China (20070335148)
文摘The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of successful cross breeding in silkworm. Protein samples from silk gland, hemolymph, and midgut were separated by 2-dimensional gel electrophoresis （2-DE）. In the three tissues the matched protein spots between Jinqiu and its cross parents were approximately 70% with approximately 30% specific protein spots. In the matched protein spots, 9-24% was differentially expressed representing up- and down-regulated expression. These specific protein spots might be either the newly appeared, which were produced from the genic interaction of cross parents＇ genes in cross breeding, or posttranscriptionally modified, which were produced from the different modifications on the same original proteins. These results indicate that it is important for a new successful breed, by cross breeding, relying on the actions of some newly produced functional proteins from genic interaction, in addition to marshaling excellent genes of cross parents.
基金Supported by Earmarked Fund for China Agriculture Research System(CARS-18)Hunan Provincial Science and Technology Support Program(2013NK3071)+1 种基金Natural Science Foundation of Hunan Province(2017JJ2137)Silkworm Germplasm Resources Multi-element Application Research and Development Innovation Team(2017XC01)
文摘The breeding activities aimed to create a natural green cocoon variety for spring rearing with the characteristics of stable natural cocoon color,easy rearing and good silk quality.Four different lines from the excellent silkworm variety Jingsong×Haoyue were taken as recurrent parents,using the natural green cocoon variety G 9202 as donor parent.After other excellent parents were introduced to cross with these different lines,four new breeding materials were selected by recurrent backcross breeding.The characters of these breeding materials showed not only natural green cocoon but also excellent comprehensive traits.A naturally colored cocoon quaternary hybrid with excellent practical characters was selected based on multiple hybrids.It is suitable for spring rearing in Yangtze valley which includes Hunan Province.It was approved by Hunan Provincial Crop Variety Approval Committee and designated Xiangcailu No.1 in 2016.
文摘家蚕血液型脓病是由家蚕核型多角体病毒(Bombyx mori nuclear polyhedrosis virus,BmNPV)引起的一种病害,症状表现为体壁紧张发亮,高节肿胀;行动狂躁,常爬行于蚕座四周;最后体壁破裂,流出脓汁而死,这会严重影响家蚕的生长和发育。为了有效防治家蚕血液型脓病,研究人员通过选育抗血液型脓病家蚕品种,取得了一定的进展。本文综述了BmNPV的特点、传播途径和致病机理等方面,并重点介绍了抗血液型脓病家蚕品种的选育方法和进展。通过梳理近几年来相关文献,总结现有抗血液型脓病家蚕品种的选育策略和主要特点,并对未来的研究方向进行了展望。本文对于家蚕血液型脓病的防治以及抗血液型脓病家蚕品种的选育具有一定的参考价值。
