The pepsin and trypsin activities and some of the properties of the two enzymes of southern sheatfish larvae were studied. The results were as follows: the highest level of trypsin activity is in the foregut in all...The pepsin and trypsin activities and some of the properties of the two enzymes of southern sheatfish larvae were studied. The results were as follows: the highest level of trypsin activity is in the foregut in all measured tissues; from foregut to hindgut, trypsin activities decrease; the pH optimum of trypsin activity is pH9.0; the strongest pepsin activity is in the stomach; the proper density of haemoglobin for detecting pepsin activity is 1.0%. These data are useful in solving applied nutritional problems, such as the adequacy of artificial food to the digestive abilities of the fish.展开更多
Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation...Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation and meat quality of southern catfish(Silurus soldatovi meridionalis Chen).Juvenile southern catfish(18.35±0.04 g)were randomly allocated into 24 cages(30 juveniles per cage),and fed diets with different dietary xanthophyll levels(at 14,42,80,108,126 and 152 mg/kg,dry matter of diet)twice daily for 8 weeks.Results indicated that the diet with 80 mg/kg xanthophyll induced a higher specific growth rate(SGR),feed efficiency ratio and protein productive value.Moreover,the 80 mg/kg xanthophyll diet also increased complement 3,immunoglobulin M(IgM)and lysozyme content more than the other groups.The mRNA expression level of inflammation-related genes and antioxidant enzyme activities further confirmed the effects of 80 mg/kg dietary xanthophyll on improving immune response.The present study also found that the 126 mg/kg xanthophyll diet significantly enhanced the content of total carotenoids and xanthophyll,hydroxyproline,collagen and amino acid in muscle.The diet with 126 mg/kg xanthophylls also induced lower drip loss,thawing loss,centrifugal loss,cooking loss and higher muscle adhesiveness,cohesiveness,springiness,gumminess and chewiness than the other treatments.In conclusion,quadratic regression model analysis based on SGR and IgM revealed that the optimum xanthophyll level in the diet was 86.78 and 84.63 mg/kg,respectively.Moreover,broken line regression analysis based on xanthophyll content in dorsal skin and chewiness in muscle demonstrated that the optimal xanthophyll level was between 89.73 and 108.66 mg/kg in the diet of southern catfish(S.soldatovi meridionalis Chen).展开更多
文摘The pepsin and trypsin activities and some of the properties of the two enzymes of southern sheatfish larvae were studied. The results were as follows: the highest level of trypsin activity is in the foregut in all measured tissues; from foregut to hindgut, trypsin activities decrease; the pH optimum of trypsin activity is pH9.0; the strongest pepsin activity is in the stomach; the proper density of haemoglobin for detecting pepsin activity is 1.0%. These data are useful in solving applied nutritional problems, such as the adequacy of artificial food to the digestive abilities of the fish.
基金supported by the Foundation and Applied Basic Research Fund Project of Guangdong Province(2019A1515110606)Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai).
文摘Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation and meat quality of southern catfish(Silurus soldatovi meridionalis Chen).Juvenile southern catfish(18.35±0.04 g)were randomly allocated into 24 cages(30 juveniles per cage),and fed diets with different dietary xanthophyll levels(at 14,42,80,108,126 and 152 mg/kg,dry matter of diet)twice daily for 8 weeks.Results indicated that the diet with 80 mg/kg xanthophyll induced a higher specific growth rate(SGR),feed efficiency ratio and protein productive value.Moreover,the 80 mg/kg xanthophyll diet also increased complement 3,immunoglobulin M(IgM)and lysozyme content more than the other groups.The mRNA expression level of inflammation-related genes and antioxidant enzyme activities further confirmed the effects of 80 mg/kg dietary xanthophyll on improving immune response.The present study also found that the 126 mg/kg xanthophyll diet significantly enhanced the content of total carotenoids and xanthophyll,hydroxyproline,collagen and amino acid in muscle.The diet with 126 mg/kg xanthophylls also induced lower drip loss,thawing loss,centrifugal loss,cooking loss and higher muscle adhesiveness,cohesiveness,springiness,gumminess and chewiness than the other treatments.In conclusion,quadratic regression model analysis based on SGR and IgM revealed that the optimum xanthophyll level in the diet was 86.78 and 84.63 mg/kg,respectively.Moreover,broken line regression analysis based on xanthophyll content in dorsal skin and chewiness in muscle demonstrated that the optimal xanthophyll level was between 89.73 and 108.66 mg/kg in the diet of southern catfish(S.soldatovi meridionalis Chen).
