期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
五条蚋和双齿蚋nrDNA-ITS区序列分析(双翅目:蚋科) 被引量:5
1
作者 杨明 毕光辉 陈汉彬 《寄生虫与医学昆虫学报》 CAS 2004年第4期219-222,共4页
测定我国两个常见吸血蚋种五条蚋和双齿蚋各 4个克隆nrDNAITS区 (包括ITS1、ITS2和 5 8SrRNA基因 )序列及其两侧的 18S和 2 8SrRNA基因部分序列。两蚋种 5 8SrRNA基因大小均为 12 2bp。五条蚋ITS1和ITS2大小分别为 136bp和 32 3bp。... 测定我国两个常见吸血蚋种五条蚋和双齿蚋各 4个克隆nrDNAITS区 (包括ITS1、ITS2和 5 8SrRNA基因 )序列及其两侧的 18S和 2 8SrRNA基因部分序列。两蚋种 5 8SrRNA基因大小均为 12 2bp。五条蚋ITS1和ITS2大小分别为 136bp和 32 3bp。双齿蚋ITS1和ITS2大小分别为 10 1~ 10 5bp和 32 1~ 32 4bp。五条蚋不同克隆ITS1 ITS2拼接序列的同源性为 99% ,双齿蚋则为 96 %~ 10 0 % 。 展开更多
关键词 ITs2 RRNA基因 ITs1 DNA 常见 变异型 克隆 ITs区序列 蚋科 双翅目
下载PDF
Quasi-likelihood techniques in a logistic regression equation for identifying Simulium damnosum s.l.larval habitats intra-cluster covariates in Togo 被引量:1
2
作者 Benjamin G.JACOB Robert J.NOVAK +5 位作者 Laurent TOE Moussa S.SANFO Abena N.AFRIYIE Mohammed A.IBRAHIM Daniel A.GRIFFITH Thomas R.UNNASCH 《Geo-Spatial Information Science》 SCIE EI 2012年第2期117-133,共17页
The standard methods for regression analyses of clustered riverine larval habitat data of Simulium damnosum s.l.a major black-fly vector of onchoceriasis,postulate models relating observational ecological-sampled para... The standard methods for regression analyses of clustered riverine larval habitat data of Simulium damnosum s.l.a major black-fly vector of onchoceriasis,postulate models relating observational ecological-sampled parameter estimators to prolific habitats without accounting for residual intra-cluster error correlation effects.Generally,this correlation comes from two sources:(1)the design of the random effects and their assumed covariance from the multiple levels within the regression model and(2)the correlation structure of the residuals.Unfortunately,inconspicuous errors in residual intracluster correlation estimates can overstate precision in forecasted S.damnosum s.l.riverine larval habitat explanatory attributes regardless how they are treated(e.g.independent,autoregressive,Toeplitz,etc.).In this research,the geographical locations for multiple riverine-based S.damnosum s.l.larval ecosystem habitats sampled from two preestablished epidemiological sites in Togo were identified and recorded from July 2009 to June 2010.Initially,the data were aggregated into PROC GENMOD.An agglomerative hierarchical residual cluster-based analysis was then performed.The sampled clustered study site data was then analyzed for statistical correlations using monthly biting rates(MBR).Euclidean distance measurements and terrain-related geomorphological statistics were then generated in ArcGIS.A digital overlay was then performed also in ArcGIS using the georeferenced ground coordinates of high and low density clusters stratified by annual biting rates(ABR).The data was overlain onto multitemporal sub-meter pixel resolution satellite data(i.e.QuickBird 0.61m wavbands).Orthogonal spatial filter eigenvectors were then generated in SAS/Geographic Information Systems(GIS).Univariate and nonlinear regression-based models(i.e.logistic,Poisson,and negative binomial)were also employed to determine probability distributions and to identify statistically significant parameter estimators from the sampled data.Thereafter,Durbin–Watson statistics were used to test the null hypothesis that the regression residuals were not autocorrelated against the alternative that the residuals followed an autoregressive process in AUTOREG.Bayesian uncertainty matrices were also constructed employing normal priors for each of the sampled estimators in PROC MCMC.The residuals revealed both spatially structured and unstructured error effects in the high and low ABR-stratified clusters.The analyses also revealed that the estimators,levels of turbidity,and presence of rocks were statistically significant for the high-ABR-stratified clusters,while the estimators distance between habitats and floating vegetation were important for the low-ABR-stratified cluster.Varying and constant coefficient regression models,ABRstratified GIS-generated clusters,sub-meter resolution satellite imagery,a robust residual intra-cluster diagnostic test,MBR-based histograms,eigendecomposition spatial filter algorithms,and Bayesian matrices can enable accurate autoregressive estimation of latent uncertainity affects and other residual error probabilities(i.e.heteroskedasticity)for testing correlations between georeferenced S.damnosum s.l.riverine larval habitat estimators.The asymptotic distribution of the resulting residual adjusted intra-cluster predictor error autocovariate coefficients can thereafter be established while estimates of the asymptotic variance can lead to the construction of approximate confidence intervals for accurately targeting productive S.damnosum s.l.habitats based on spatiotemporal field-sampled count data. 展开更多
关键词 simulium damnosum s.l.clus.er covariates QuickBird Onchoceriasis Annual biting rates Bayesian TOGO
原文传递
蚋类幼期(幼虫和蛹)的生态观察 被引量:1
3
作者 安继尧 严格 +3 位作者 郝宝善 杨礼贤 刘查田 麦振全 《军事医学科学院院刊》 CSCD 北大核心 1992年第2期111-114,共4页
该文报道在广州市郊采获蚋的种类及其主要蚋种幼期的季节消长。在调查发现的4种蚋中,以黄足真蚋(Simulium(Eus imul ium)aureohirtum)为最多,其幼虫数占采获幼虫总数的94.2%,五条蚋(S.(Simulium)quinauest r iatum)占4.8%,后宽绳蚋(S.(G... 该文报道在广州市郊采获蚋的种类及其主要蚋种幼期的季节消长。在调查发现的4种蚋中,以黄足真蚋(Simulium(Eus imul ium)aureohirtum)为最多,其幼虫数占采获幼虫总数的94.2%,五条蚋(S.(Simulium)quinauest r iatum)占4.8%,后宽绳蚋(S.(Gomphost il bia)metatarsale)和手指蚋(S.(S)digi tatum)为数甚少。黄足真蚋幼虫和蛹在该溪流中终年均可生长发育,全年于5月和11月出现两个较大的数量高峰。 展开更多
关键词 黄足真蚋 五条纳 幼虫 季节消长
原文传递
3种吸血蚋酯酶同工酶的比较研究 被引量:3
4
作者 高博 张建庆 +7 位作者 蔡亨忠 王光辉 范建华 黄健群 黄恩炯 王宇平 张述铿 王公东 《中国媒介生物学及控制杂志》 CAS CSCD 2009年第2期138-139,共2页
目的探讨酯酶(EST)同工酶酶谱特征在蚋类分类上的价值,并为蚋类系统发育的研究积累生物化学方面的基础资料。方法应用垂直板不连续聚丙烯酰胺凝胶电泳技术,对素木蚋、双齿蚋和五条蚋进行EST同工酶电泳,测量并计算各酶带的迁移率。结果3... 目的探讨酯酶(EST)同工酶酶谱特征在蚋类分类上的价值,并为蚋类系统发育的研究积累生物化学方面的基础资料。方法应用垂直板不连续聚丙烯酰胺凝胶电泳技术,对素木蚋、双齿蚋和五条蚋进行EST同工酶电泳,测量并计算各酶带的迁移率。结果3蚋种的EST同工酶酶谱共显示10条酶带,种间存在明显差异,具有各自的特征性酶谱,种内个体之间、幼虫与蛹之间酶谱主带相同,非主带略有差异。结论EST同工酶酶谱可作为蚋种鉴定的特征之一。 展开更多
关键词 素木蚋 双齿蚋 五条蚋 电泳 酯酶同工酶
原文传递
基于COI基因分析的双齿蚋分子分类研究
5
作者 罗洪斌 何艳霞 +1 位作者 陈晓丹 樊莎莎 《基因组学与应用生物学》 CAS CSCD 北大核心 2019年第5期1977-1982,共6页
为了确定COI基因序列分析在蚋科中的分类地位,本研究测定了双齿蚋(Simulium bidentatum) COI基因序列,分析该基因序列特征;下载与其同源性较高的12个蚋种的COI基因序列15条,进行同源性分析和分子系统发育研究。结果显示,双齿蚋COI基因序... 为了确定COI基因序列分析在蚋科中的分类地位,本研究测定了双齿蚋(Simulium bidentatum) COI基因序列,分析该基因序列特征;下载与其同源性较高的12个蚋种的COI基因序列15条,进行同源性分析和分子系统发育研究。结果显示,双齿蚋COI基因序列(DQ534946)与S. notiale、S. snowi、S. nyssa等蚋种的COI基因序列同源性最高,在贝叶斯分析所构建的分子系统进化树中,不同蚋组的序列各自聚成一枝,双齿蚋序列(DQ534946)未与其他任何蚋种聚为一枝。研究结果表明,双齿蚋COI基因序列与蚋亚属序列同源性较高,COI基因序列分析能较好建立蚋亚属内组间关系,双齿蚋未与其他蚋组序列聚为一枝,分类至淡足蚋组并不合适,支持分类至阿根蚋组。本研究进一步明确了COI基因序列分析在蚋科分子系统发育研究中的意义,并在一定程度上解决了双齿蚋在蚋科的分类争议。 展开更多
关键词 双齿蚋 COI基因 分子分类
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部