Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene seri...Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.展开更多
[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group ...[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group （ cyclophosphamide group ）, negative control group （ physiological saline group）, high-dose A. moles Hance group （30 g/kg）, moderate-dose A. mollis Hance group （20 g/kg） and low-dose A. mollis Hance group （10 g/kg）. Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular cells were analyzed by using single cell gel electrophoresis assay, to investigate the effect of A. mollis Hance on DNA in mouse cells. [Result] Compared with positive control group, Tail DNA% and Tail Moment of moose liver, kidney, lung and testicular cells in A. moles Hance groups were significantly lower （ P 〈 0.01 ）. Compared with negative control group, Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular ceils in high-dose A. mollis Hance group were significantly lower （ P 〈 0.01 ）, while the other A. mollis Hance groups showed no statistically significant difference （ P 〉0.05 ）. [ Conclusion] A. mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range.展开更多
目的研究人工合成偶氮类色素胭脂红对体外培养小鼠胚胎成纤维细胞株(NIH3T3)DNA的损伤作用。方法单层培养的3T3细胞中加入不同浓度胭脂红培养4 h,用碱性单细胞凝胶电泳技术(single cell gel elec-trophoresis,SCGE)检测3T3细胞DNA的单...目的研究人工合成偶氮类色素胭脂红对体外培养小鼠胚胎成纤维细胞株(NIH3T3)DNA的损伤作用。方法单层培养的3T3细胞中加入不同浓度胭脂红培养4 h,用碱性单细胞凝胶电泳技术(single cell gel elec-trophoresis,SCGE)检测3T3细胞DNA的单链断裂(single-strand breaks,SSBs)情况。结果各染毒组彗星细胞率、彗星细胞的尾长、尾部DNA含量及尾矩与胭脂红浓度正相关,呈剂量-反应关系。结论胭脂红对体外培养3T3细胞DNA具有损伤作用,浓度越大,损伤越严重。展开更多
In this study, we sought to elucidate the process of DNA degradation in brain and dental pulp cells of mice, within postmortem 0-72 hours, by using the single cell gel electrophoresis assay and professional comet imag...In this study, we sought to elucidate the process of DNA degradation in brain and dental pulp cells of mice, within postmortem 0-72 hours, by using the single cell gel electrophoresis assay and professional comet image analysis and processing techniques. The frequency of comet-like cells, the percentage of tail DNA, tail length, tail moment, Olive moment and tail area increased in tandem with increasing postmortem interval. In contrast, the head radius, the percentage of head DNA and head area showed a decreasing trend. Linear regression analysis revealed a high correlation between these parameters and the postmortem interval. The findings suggest that the single cell gel electrophoresis assay is a quick and sensitive method to detect DNA degradation in brain and dental pulp cells, providing an objective and accurate new way to estimate postmortem interval.展开更多
Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedu...Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedure.Methods:Clinical charts of 76 patients who underwent operation under general anesthesia and 76 healthy control subjects with documented results of DNA damage extent in PBMCs from the single-cell gel electrophoresis(SCGE) or cornel assay and scrum contents of superoxide dismutase(SOD) and malondialdehyde(MDA) from biochemical analyses were reviewed.The percentage of comet PBMCs and tail DNA and semm contents of SOD and MAD were analyzed by student t-test.Results:Compared with health) control subjects, generally anesthetized surgical patients had significantly higher%comet PBMCs and%tail DNA(P【0.05) and significantly lower serum concentrations of SOD(P【0.05) and significantly higher serum concentrations of MAD(P【0.05).Compared with levels before general anesthesia in surgical patients,%comet PBMCs,%tail DNA.and serum lexels of MAD were significantly higher(P【0.05 or 0.01).and semm levels of SOD were significantly lower(P【0.05).alter general anesthesia.Conclusions:General anesthesia during surgery causes a certain degree of hypoxia and PBMC damage.Particular attention should be paid to monitoring and maintenance of blood oxygen saturation in patients undergoing surgery under general anesthesia.展开更多
文摘Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.
基金Supported by Scientific Research Project from Guangxi Department of Education(200710MS052)Project from Technology Bureau of Yulin City(0881038)
文摘[ Objective] This study aimed to evaluate the genotoxicity ofAbrus mollis Hance by using single cell gel electrophoresis. [Method] Forty mice were di- vided into five groups randomly, including positive control group （ cyclophosphamide group ）, negative control group （ physiological saline group）, high-dose A. moles Hance group （30 g/kg）, moderate-dose A. mollis Hance group （20 g/kg） and low-dose A. mollis Hance group （10 g/kg）. Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular cells were analyzed by using single cell gel electrophoresis assay, to investigate the effect of A. mollis Hance on DNA in mouse cells. [Result] Compared with positive control group, Tail DNA% and Tail Moment of moose liver, kidney, lung and testicular cells in A. moles Hance groups were significantly lower （ P 〈 0.01 ）. Compared with negative control group, Tail DNA% and Tail Moment of mouse liver, kidney, lung and testicular ceils in high-dose A. mollis Hance group were significantly lower （ P 〈 0.01 ）, while the other A. mollis Hance groups showed no statistically significant difference （ P 〉0.05 ）. [ Conclusion] A. mollis Hance has no damage effect on DNA in mouse cells within this experimental dose range.
基金supported by Key Research Plan of the Ministry of Public Security of China, No. 2011ZDYJXJXY005Scientific Research Foundation of the Higher Education Institutions of Liaoning Province, China, No. 2008Z205
文摘In this study, we sought to elucidate the process of DNA degradation in brain and dental pulp cells of mice, within postmortem 0-72 hours, by using the single cell gel electrophoresis assay and professional comet image analysis and processing techniques. The frequency of comet-like cells, the percentage of tail DNA, tail length, tail moment, Olive moment and tail area increased in tandem with increasing postmortem interval. In contrast, the head radius, the percentage of head DNA and head area showed a decreasing trend. Linear regression analysis revealed a high correlation between these parameters and the postmortem interval. The findings suggest that the single cell gel electrophoresis assay is a quick and sensitive method to detect DNA degradation in brain and dental pulp cells, providing an objective and accurate new way to estimate postmortem interval.
文摘Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedure.Methods:Clinical charts of 76 patients who underwent operation under general anesthesia and 76 healthy control subjects with documented results of DNA damage extent in PBMCs from the single-cell gel electrophoresis(SCGE) or cornel assay and scrum contents of superoxide dismutase(SOD) and malondialdehyde(MDA) from biochemical analyses were reviewed.The percentage of comet PBMCs and tail DNA and semm contents of SOD and MAD were analyzed by student t-test.Results:Compared with health) control subjects, generally anesthetized surgical patients had significantly higher%comet PBMCs and%tail DNA(P【0.05) and significantly lower serum concentrations of SOD(P【0.05) and significantly higher serum concentrations of MAD(P【0.05).Compared with levels before general anesthesia in surgical patients,%comet PBMCs,%tail DNA.and serum lexels of MAD were significantly higher(P【0.05 or 0.01).and semm levels of SOD were significantly lower(P【0.05).alter general anesthesia.Conclusions:General anesthesia during surgery causes a certain degree of hypoxia and PBMC damage.Particular attention should be paid to monitoring and maintenance of blood oxygen saturation in patients undergoing surgery under general anesthesia.
