Two new polyhydroxysteroids, 12β,16β,20-trihydroxycholesta-1,4-dien-3-one 16-acetate（1） and 24- methyl-12β,16β,20-trihydroxycholesta-1,4-dien-3-one（2） were isolated from octocoral Sinularia sp. Their structure...Two new polyhydroxysteroids, 12β,16β,20-trihydroxycholesta-1,4-dien-3-one 16-acetate（1） and 24- methyl-12β,16β,20-trihydroxycholesta-1,4-dien-3-one（2） were isolated from octocoral Sinularia sp. Their structures were elucidated primarily on the basis of 1D and 2D NMR and the mass spectroscopic studies. The cytotoxicity of these compounds against three tumor cell lines was also reported.展开更多
One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ...One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ9(15)-africanene(6),and methyl(5′E)-5-(2′,6′-dimethylocta-5′,7′-dienyl)furan-3-carboxylate(7)were isolated from the Okinawan soft coral Sinularia sp.Their chemical structures were elucidated based on spectroscopic analysis(FTIR,NMR,and HRESIMS),and the relative stereochemistry of 1 was determined by NOESY experiments and acetylation,which yielded derivative 2.In addition,compounds 1 and 7 exhibited toxicity in the brine shrimp lethality test.展开更多
热休克蛋白70(heat shock protein 70, HSP70)在生物细胞或组织免受热或氧化应激等方面起着关键作用,是已知高度保守的蛋白质之一。由于全球环境持续升温,珊瑚大面积白化、死亡,珊瑚如何应对持续升温的抗逆机制是科学研究热点。本研究...热休克蛋白70(heat shock protein 70, HSP70)在生物细胞或组织免受热或氧化应激等方面起着关键作用,是已知高度保守的蛋白质之一。由于全球环境持续升温,珊瑚大面积白化、死亡,珊瑚如何应对持续升温的抗逆机制是科学研究热点。本研究从高温胁迫短指软珊瑚测序蛋白序列数据库分析鉴定出了28个HSP70蛋白家族成员,均为酸性亲水蛋白,大部分蛋白质结构较为稳定。亚细胞定位表明HSP70蛋白主要分布在珊瑚细胞核、细胞质中,在线粒体、内质网上也有少量分布。信号肽预测表明, 28个HSP70蛋白成员中26个没有信号肽,大部分不属于分泌蛋白,不存在跨膜结构。系统进化树结果表明短指软珊瑚HSP70蛋白家族成员聚成5大类。短指软珊瑚HSP70蛋白家族结构和保守基序分析中预测到了10条保守基序motif分为5个亚族。短指软珊瑚HSP70蛋白家族二级结构主要以α-螺旋和无规则卷曲为主,α-螺旋的含量占比大。28个HSP70家族蛋白中有25个预测到了N-糖基化位点,且位点个数在1~9范围内。28个HSP70家族蛋白均预测到磷酸化位点和O-糖基化位点,总个数分别在41~96和1~23范围内。本研究HSP70家族蛋白结果为今后珊瑚在应对全球升温胁迫中的适应机制等方面研究奠定了基础。展开更多
基金Supported by the National Natural Science Foundation of China(No.20772048)
文摘Two new polyhydroxysteroids, 12β,16β,20-trihydroxycholesta-1,4-dien-3-one 16-acetate（1） and 24- methyl-12β,16β,20-trihydroxycholesta-1,4-dien-3-one（2） were isolated from octocoral Sinularia sp. Their structures were elucidated primarily on the basis of 1D and 2D NMR and the mass spectroscopic studies. The cytotoxicity of these compounds against three tumor cell lines was also reported.
文摘One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ9(15)-africanene(6),and methyl(5′E)-5-(2′,6′-dimethylocta-5′,7′-dienyl)furan-3-carboxylate(7)were isolated from the Okinawan soft coral Sinularia sp.Their chemical structures were elucidated based on spectroscopic analysis(FTIR,NMR,and HRESIMS),and the relative stereochemistry of 1 was determined by NOESY experiments and acetylation,which yielded derivative 2.In addition,compounds 1 and 7 exhibited toxicity in the brine shrimp lethality test.
