Effect of recombinant human epithelium growth factor on livability of skin graft

AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed and skin grafted,the rhEGF was injected under the skin graft.80 cases without injection of rhEGF were made as contrast.Ten days later,the area of survived skin was measured and the livability of skin was calculated. RESULTS: The skin livability of cases with in jection of rhEGF was (90.67±10.02)% and the skin livability of contrast cases was(76.85±8.35)%. There axisted evident differences between them(P< 0.01).CO NCLUSION:The rhEGF was an effective method for increasing livability of skin gra ft.

Relation between total skin factor and individual layer skin factor in well test of multilayered system

Formation damage evaluation is an important component in well test analysis and for- mation evaluation. Formation damage of one-layer reservoir has been studied by decomposing the total skin factor and estimating the mud pollution depth. And the well test analyses for multilayered reservoirs were reported, but relation between the total skin factor and individual layer skin factor has not been considered. The relation is devel-oped in this note with well test analysis combining numerical simulation, which aids properly evaluating formation and making stimulating decision.

Biomarkers of skin toxicity induced by anti-epidermal growthfactor receptor antibody treatment in colorectal cancer

Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced by such antibodies negatively impact on the quality of life of patients and decreases drug compliance during treatment. If we can predict the high-risk group susceptible to severe skin toxicity before treatment, we can undertake the early management of any arising skin disorders and formulate a more accurate prognosis for anti-EGFR antibody treatment. Previous studies have identified molecular markers of skin toxicity induced by anti-EGFR antibody, such as EGFR polymorphisms, the expression of inflammatory chemokines and serum levels of EGFR ligands. A clinical trial was undertaken involving the escalation of cetuximab doses, guided by the grade of skin toxicity observed, such as no or low-grade, in metastatic colorectal cancer(the EVEREST study). The dose escalation of cetuximab was confirmed by a safety profile and had the tendency to achieve a higher response rate in KRAS wild-type patients. A large, prospective randomized trial is now ongoing(EVEREST 2) and the results of this trial may contribute to personalized medicine in KRAS wild-type colorectal cancer patients.

A STUDY OF mRNA EXPRESSION OF GROWTH FACTORS IN FULL-THICKNESS BURNT RATS AFTER INTERMINGLEDTRANSPLANTATION OF AUTO-ALLOGRAFT SKIN

Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and allografts on burnt rats were quantitatively analysed. Results (1) mRNA expressions ofall the four growth factors mentioned above were found both in allografts and autografts, the expressions beinghigher in autografts than allografts; (2) In autografts, TGFα expression began to rise from second post transplantday (D2), reaching a peak on D18,. TGFβ1 expression gained a high level on D2 and had itS peak on D18; PDGFexpression had its peak on D4, rebounding on D25; bFGF expression, exceedingly high on D2, dropped andrebounded in the same manner as in PDGF; (3) In the early stage of the wound healing, mRNA expression of thefour growth factors was lower in allografts, rising up in varying degrees thereafter. Conclusion (1) Graftsurvival took place both in autografts and allografts, with the former pederming better than the latter; (2) Thecombining action of growth factors that reached the affected area through autosecretion and bysecretion probablypromoted the healing of the burn wound, following signal transmission channels; (3) Rejection, which occurred inallogralts, was responsible for the decreasing viability of allografts and their replacement by the host skin.

Evaluation of Epidermal Growth Factor-Incorporating Skin Care Product in Culture Experiment Using Human Fibroblasts

This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.

外源性碱性成纤维细胞生长因子促进大鼠创面愈合的机制

背景:深入揭示外源性碱性成纤维细胞生长因子促进创面愈合的分子机制。目的:探讨外源性碱性成纤维细胞生长因子对大鼠创面修复中巨噬细胞表型转换和肉芽再生的影响。方法:(1)体外细胞实验:分为正常对照组、低剂量碱性成纤维细胞生长因子组、高剂量碱性成纤维细胞生长因子组以及碱性成纤维细胞生长因子+丙戊酸组,其中低、高剂量碱性成纤维细胞生长因子组细胞培养基中分别添加100,200μg/L碱性成纤维细胞生长因子,碱性成纤维细胞生长因子+丙戊酸组细胞培养基中添加200μg/L碱性成纤维细胞生长因子和20 mmol/L Notch1/Jagged1激动剂丙戊酸。通过EdU实验、划痕实验、小管生成实验检测碱性成纤维细胞生长因子对人脐静脉内皮细胞增殖、迁移和血管新生的影响。(2)体内动物实验:SD大鼠按照随机数字表法分为模型组、低剂量碱性成纤维细胞生长因子组、高剂量碱性成纤维细胞生长因子组以及碱性成纤维细胞生长因子+丙戊酸组,构建大鼠全层皮肤缺损开放性创面模型,其中低、高剂量碱性成纤维细胞生长因子组皮下注射100,200μg/L碱性成纤维细胞生长因子,碱性成纤维细胞生长因子+丙戊酸组大鼠皮下注射200μg/L碱性成纤维细胞生长因子的同时腹腔注射10 mg/kg丙戊酸。给药7,14 d检测大鼠创面的愈合率;TUNEL检测创面组织中的细胞凋亡情况;酶联免疫吸附实验检测大鼠血清中丙二醛、超氧化物歧化酶、肿瘤坏死因子α和白细胞介素10水平;免疫荧光检测创面组织中巨噬细胞的表型转换情况;免疫组化检测创面组织中增殖细胞核抗原、CD31和血管内皮生长因子的表达;Western blot法检测创面组织中Notch1、Jagged1的表达。结果与结论:(1)与正常对照组相比,碱性成纤维细胞生长因子能明显促进人脐静脉内皮细胞的增殖、迁移和血管新生,并且具有剂量依赖性;(2)与模型组相比,碱性成纤维细胞生长因子能明显促进创面的愈合,下调创面组织中的细胞凋亡率;降低大鼠血清中丙二醛和肿瘤坏死因子α水平,升高超氧化物歧化酶和白细胞介素10水平;促使创面组织中巨噬细胞向M2型转换,上调创面组织中增殖细胞核抗原、CD31和血管内皮生长因子的表达;抑制创面组织中Notch1、Jagged1的表达,并且均具有剂量依赖性。丙戊酸可部分逆转碱性成纤维细胞生长因子对创面愈合的促进作用。结果表明,碱性成纤维细胞生长因子能明显促进创面愈合与肉芽再生以及诱导巨噬细胞向M2型转换,这可能与调控Notch1/Jagged1信号有关。

Influencing Factors of Fabric-Skin Adhesion Based on Gray Correlation Analysis Method

Fabric-skin adhesion was objectively described by the indices of the maximum adhesion force F_(max) , the maximum separation distance L_(max) ,and adhesion work W as well as the adhesion force-separation distance curve. Firstly,gray correlation analysis method was adopted to investigate the correlation levels between adhesion indices,and secondly the relative importance of fabric structural parameters to fabric-skin adhesion,as well as the correlation levels between skin adhesion, water absorption, and wicking properties of the fabric. The results prove that W exhibits clear correlations with both F_(max) and L_(max) , yet the relevance between F_(max) and L_(max) is weak. Fabric adhesion indices are most associated with fabric mass and least with fabric thickness,whereas fabric wicking and water absorption present closest correlation with fabric thickness. Therefore, it is concluded that the relevance between fabric wicking, water absorption, and skin-adhesion properties are rather comprehensive than straight.

神经系统在调控皮肤创伤修复和瘢痕愈合中的重要作用

背景:皮肤创伤修复是一个极其复杂的过程,神经系统在调控创伤修复和瘢痕愈合方面发挥重要作用。目的:对皮肤创伤修复过程中神经系统的作用,包括相关因子、多种细胞对损伤皮肤修复的作用进行综述。方法:检索PubMed及中国知网数据库2003-2024年发表的文献,英文检索词为“skin wound healing,nervous system,nerve regeneration,growth factor”,中文检索词为“皮肤创伤修复,皮肤神经支配,神经肽,生长因子,细胞协同作用”。最终筛选出83篇文献进行分析。结果与结论:①皮肤受到自主神经和感觉神经的密集支配,越来越多的证据表明,皮肤神经系统不仅负责向中枢神经系统传递感觉信息,而且在包括伤口愈合在内的各种皮肤功能中发挥重要的作用。②神经系统作用于皮肤创伤愈合的不同阶段,能够调节血管再生,促进相关生长因子及神经递质释放,和不同类型细胞协同作用从而促进皮肤创面愈合;因此,研究神经系统作用的潜在机制成为促进皮肤创伤愈合的有力靶点。

Current patterns of the sun protection measures adopted by nurses and the risk factors influencing their compliance

The incidence of skin cancer is increasing worldwide. Included in high risk group are people who have light common phenotypic factors (hair, skin, eyes), have high number of naevi, report previous sunburns and have a family history of skin cancer. Protecting the skin from the sun by wearing protective clothing, using sunscreen and avoiding sun exposure are recommended among primary preventive activities. The purpose of this study was to describe the current patterns of sun protection measures adopted by nurses and to examine the association between compliance with sun protection measures and the main risk factors of melanoma. This cross sectional study was conducted in 2007 during the Annual Greek National Conference of Nursing. Nurses most commonly chose to use sunscreen, to wear glasses, to use an umbrella or to seek for shade in order to protect themselves against sun exposure. Finally, phenotypic factors, previous sunburns and common naevi presence were found to affect nurses’ attitude towards protective measures. This study poses the necessity of targeting high risk groups for melanoma through campaigns aiming to foster sun protection behaviours.

Tissue-Engineered Products for Skin Regenerative Medicine

In a general wound healing process, foreign bodies and tissue detritus have to be broken down and then a new tissue is produced. However, the new tissue formation sometimes fails to proceed under the impaired conditions such as burn injury and intractable skin ulcer. A major obstruction to wound healing is infection. Another obstruction to wound healing is deficiency of growth factors. The endogenous levels of growth factors are reduced in some chronic wounds. To improve these wound conditions, researchers have been trying to create several types of artificial skins. The tissue-engineered products include three prime constituents, i.e., cells, growth factors, and materials. In this review, the practical design of tissue-engineered products for skin regenerative medicine is introduced. The first design makes it possible to release silver sulfadiazine (AgSD) from a wound dressing. The second design makes it possible to release Epidermal Growth Factor (EGF) from a wound dressing or a skin care product composed of hyaluronic acid spongy sheet containing bioactive ingredients. The third design makes it possible to release several types of growth factors from allogeneic fibroblasts within cultured dermal substitute. This tissue-engineered product is prepared by seeding allogeneic fibroblasts into a collagen and hyaluronic acid spongy sheet. Although allogeneic cells are rejected gradually in immune system, they are able to release some types of growth factors, thereby regenerating a damaged tissue. The clinical study demonstrates that these tissue-engineered products are promising for the treatment of burn injury and intractable skin ulcer.

Effect of Skin Pigmentation on Near Infrared Spectroscopy

The purpose of this study was to determine the effects of skin pigmentation regarding Near Infrared Spectroscopy (NIRS) tissue oxygen saturation values (StO2). The study examined NIRS values in individuals with varying skin pigmentation on the anterior compartment of the lower leg and volar forearm to determine if correlation exists among three NIRS devices, the EQUANOX, Casmed, and INVOS. Skin pigmentation was measured on the anterior lower leg (AL) and volar forearm (VF) of participants using a noninvasive colorimeter that employed reflective spectroscopy to produce a quantitative value for erythema (skin “redness”) and melanin (skin pigment). Muscle oxygenation was measured using three oximetry devices with sensors placed in the same areas. The EQUANOX device showed no significant correlation with skin pigmentation, while the Casmed and INVOS devices showed moderate and significant correlation with skin pigmentation, respectively. Different devices have different abilities to remove confounding variables, such as skin pigmentation and erythema, which may affect clinical decision-making, and affect the use of NIRS technology.

Expression of EGF and EGFR in Developing Skin of the Goat Fetus

The histological characteristics,distribution and variability of epidermal growth factor(EGF) and epidermal growth factor receptor(EGFR) during development of skin in the goat fetus were studied with histological and immunohistochemical methods.The results showed that the epidermis formed at the 6th gestational week,the thickness increased gradually because of transition from simple epithelium to stratified epithelium,and then decreased after the 15th week.The dermis was present at the 10th week,and the dermal derivatives appeared after the 11th week.All structures of the skin matured gradually during prenatal development.Low expression of EGF and EGFR could be observed at the 6th week and increased thereafter.Before the 11th week,EGF was mainly located in the cytoplasm of the basal layer of cells of epidermis,hair follicular epithelial cells and fibroblasts,and EGFR was mainly located on cell membranes of these cells.From the 11th to 16th week,the expression of EGF and EGFR extended from basal layer cells,prickle cells,hair follicular epithelial cells and fibroblasts to vascular endothelial cells,epithelial cells of sweat glands and arrector pili muscle.EGF was mainly detected cytoplasmically,and EGFR was located on membranes of these cells.From the 17th week to birth,EGF was mainly located in cells of the basal layer and hair follicular epithelial cells as the skin thinned and staining intensity continued to increase.The expressed amount of EGF and EGFR tended to increase throughout the whole development stage encompassed by gestational weeks 6 to 21 and the two components of the EGF signaling system were highly correlated.The results suggest that EGF and EGFR play important roles in the development of skin and its derivatives.

Koena, a Novel Compound That Dampens Inflammation in the Skin

Inflammatory skin diseases are characterized by the activation of the innate and adaptive immune system via the production of pro-inflammatory cytokines. The main proinflammatory cytokines responsible for this development include TNF-alpha, Interleukin 1, 6 and 17, also the transcription factor NF-kappa B. Small molecule anti-inflammatory compounds such as those detailed in the paper, show much promise in dealing with these skin disorders. Four nutraceutical molecules that are well characterized by their capacity to interact with many of the pro-inflammatory cytokines, importantly IL-17, are found in an Australian product, Koena. Koena has been shown to not only decrease these cytokines but also help increase the production of Interleukin 10 and important anti-inflammatory cytokines. This paper reports clinical benefits of Koena in a variety of circumstances;eczema and psoriasis;insect bites and sting;solar damaged skin and keratosis. An analysis of patient/purchaser reviews via the website Koena.com.au to ascertain what condition they purchased the product for, if any, and the satisfaction rating after use. More than 35% of reviewers reported purchasing Koena to help with inflamed skin. Those reporting being satisfied with the result was 95% for this use. This paper reports on the benefit of small molecule anti-inflammatories in modulating and not blocking an immune response. It also illustrates the benefits of multitherapy when dealing with complex inflammatory responses as opposed to monotherapy.

小檗碱调节HIF-1α/VEGF信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响

目的:探究小檗碱(BR)调节缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响。方法:取SD大鼠采用创面局部多次涂抹冰醋酸建立皮肤溃疡模型,随机数字法分组,包括模型组(M组)、小檗碱低剂量组(BR-L组)、小檗碱高剂量组(BR-H组)、小檗碱高剂量+空载组(BR-H+pc-DNA组)、小檗碱高剂量+HIF-1α敲低组(BR-H+pc-HIF-1αKD组),每组10只,另选10只SD大鼠作为对照组(C组)。以药物分组处理3周后,测定各组大鼠创面愈合率;HE染色检测各组大鼠皮肤溃疡创面组织病理形态;免疫组织化学染色检测各组大鼠皮肤溃疡创面组织微血管密度(MVD)及胶原蛋白Ⅰ(CollagenⅠ)与纤维结合蛋白(FN)表达;酶联免疫吸附测定(ELISA)各组大鼠血清及皮肤溃疡创面组织炎性因子IL-6、IL-8表达水平;免疫印迹法检测各组大鼠皮肤溃疡创面组织HIF-1α/VEGF通路蛋白表达水平。结果:与C组相比,M组大鼠创面组织MVD、血清及创面组织IL-6、IL-8水平升高(均P<0.05)。与M组相比,BR-L组、BR-H组、BR-H+pc-DNA组大鼠创面愈合率、创面组织MVD、HIF-1α及VEGF蛋白表达均升高(均P<0.05),血清及创面组织IL-6、IL-8水平均降低(均P<0.05),且高剂量小檗碱作用更强。敲低HIF-1α可减弱BR对模型组大鼠各指标的作用。结论:小檗碱可通过上调HIF-1α/VEGF通路而降低炎性因子表达,抑制炎症,增强皮肤溃疡大鼠血管生成和胶原形成,促进其创面愈合。

组织工程皮肤在糖尿病皮肤溃疡模型大鼠创面修复中的应用研究

目的:探讨组织工程皮肤修复糖尿病大鼠溃疡模型创面的可能机制。方法:选择腹腔注射链脲佐菌素及背部打孔建立糖尿病溃疡模型,将60只模型大鼠随机分为5组,每组各12只,对照组采用凡士林纱布覆盖创面,支架组采用无细胞纤维蛋白支架行创面移植,成纤维细胞组采用含成纤维细胞的纤维蛋白支架行创面移植,表皮干细胞组采用含表皮干细胞的纤维蛋白支架行创面移植,组织工程皮肤组采用含成纤维细胞和表皮干细胞的纤维蛋白支架行创面移植。术后第2周和第4周,比较各组创面愈合率、炎性因子[白细胞介素(IL-1)-1、IL-2、肿瘤坏死因子(TNF)-α]和相关生长因子[血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、转化生长因子(TGF)-β1]的含量。结果:术后第2周和第4周,组织工程皮肤组创面愈合率>成纤维细胞组(表皮干细胞组)>支架组>对照组(P<0.05);IL-1、IL-2和TNF-α含量为组织工程皮肤组<成纤维细胞组(表皮干细胞组)<支架组<对照组(P<0.05);VEGF、bFGF和TGF-β1含量为组织工程皮肤组>成纤维细胞组(表皮干细胞组)>支架组>对照组(P<0.05);与其他组相比,组织工程皮肤组大鼠创面新生皮肤质地和韧性良好,皮肤形态成熟,可见胶原纤维成束状排列。结论:组织工程皮肤能够促进糖尿病溃疡大鼠模型创面愈合,可能与拮抗创面炎症损伤、促进相关血管生长因子分泌有关。

气井拟压力弹性一相法的推导、简化及应用

对于一口处在封闭边界之内的新气井,以某稳定产量开井生产,进行了压降曲线测试。从理论上讲,又可将压降曲线按其压力动态划分为非稳态阶段、过渡阶段(又称为非稳态阶段的后期)和拟稳态阶段。所谓非稳态阶段,是指气井压降漏斗的外缘半径,尚未达到边界之前的范围。而所谓过渡阶段,是指由非稳态阶段过渡到拟稳态阶段的范围。对于拟稳态阶段,我国称为弹性二相法,可用于确定气井控制的原始地质储量。该法被连续四次列于国家油气的行业标准。对于非稳态阶段,本文基于达西定律的平面径向流微分式,利用AI-Hussainy提出的拟压力函数,经推导得到了拟压力的弹性一相法。利用该法可以评价气藏的有效渗透率和井的总表皮系数。同时,利用Wattenbarger对拟压力函数性质的研究结果,通过简化得到了由压力一次方和压力平方表示的弹性一相法。通过实例应用结果表示,三种方法评价的结果是一致的。

枇杷叶三萜酸联合重组人表皮生长因子对激素依赖性皮炎豚鼠皮肤屏障功能的修复及免疫失衡的影响

目的 观察枇杷叶三萜酸(TAL)联合重组人表皮生长因子(rh-EGF)对激素依赖性皮炎(HDD)豚鼠模型皮肤屏障功能修复及免疫失衡的影响。方法 选取40只无特定病原(SPF)级豚鼠,随机分为对照组、模型组、rh-EGF组、TAL组和联合组,每组各8只。除对照组外,其他各组涂抹0.05%卤米松乳膏,连续45 d,构建HDD模型。构建成功后,rh-EGF组涂抹rh-EGF凝胶,TAL组涂抹TAL溶液,联合组涂抹TAL和rh-EGF凝胶,对照组和模型组涂抹生理盐水,共15 d。记录各组临床症状并打分;检测经皮水分丢失(TEWL)、角质层含水量(WCSC)和皮脂量(SC);HE染色观察各组豚鼠皮肤组织病理变化;采用酶联免疫吸附测定(ELISA)法检测总免疫球蛋白E(IgE)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)水平。结果 与对照组比较,模型组症状明显、皮肤组织损坏严重,TEWL增加,WCSC和SC减少;免疫因子Ig E和IL-4水平升高、IFN-γ水平降低(P<0.05)。与模型组比较,rh-EGF组、TAL组和联合组用药后临床症状减轻、皮肤组织损伤不明显;TEWL减少,WCSC和SC增加,免疫因子IgE和IL-4水平降低、IFN-γ水平升高(P<0.05)。且联合组与rh-EGF组和TAL组比较,作用效果更加明显(P<0.05)。TAL组和联合组各指标比较,差异无统计学意义(P>0.05)。结论 TAL联合rh-EGF可有效缓解HDD豚鼠的临床症状,改善皮肤细胞损伤,修复皮肤屏障功能,提升免疫能力。

老年重症患者急性皮肤衰竭现状及危险因素分析

目的 探讨老年重症患者急性皮肤衰竭现状,并分析其危险因素。方法 采取前瞻性观察性研究的方法,将2021年1月-2023年1月入住苏州市某三级甲等医院急诊重症监护室的老年患者作为研究对象,收集患者住院期间的病例资料,采用单因素及多因素Logistic回归分析老年重症患者急性皮肤衰竭发生的危险因素。结果 251例老年患者完成调查,105例发生了急性皮肤衰竭,发生率为41.83%。Logistic回归分析结果显示,老年重症患者急性皮肤衰竭发生的危险因素包括Braden评分(OR=0.732)、肾脏衰竭(OR=2.881)、心力衰竭(OR=3.962)、脓毒症(OR=2.459)、动脉血乳酸水平(OR=1.226)、镇静镇痛(OR=3.135)。结论 老年重症患者急性皮肤衰竭的发生率较高,低Braden评分、肾脏衰竭、心力衰竭、脓毒症、动脉血乳酸水平高、使用镇静镇痛药物是老年重症患者急性皮肤衰竭发生的独立危险因素。

根治性膀胱切除术后患者发生尿路造口周围潮湿相关性皮肤损伤的影响因素

目的探讨根治性膀胱切除术后患者发生尿路造口周围潮湿相关性皮肤损伤(PMASD)的影响因素。方法选取100例行根治性膀胱切除术治疗的膀胱癌患者作为研究对象,根据术后3个月是否发生PMASD分为发生组与未发生组。收集两组患者的一般资料和临床资料,分析根治性膀胱切除术后患者发生PMASD的影响因素,并绘制受试者工作特征(ROC)曲线评估影响因素的预测价值。结果根治性膀胱切除术后患者PMASD的发生率为32.00%(32/100)。相比于未发生组,发生组的体质指数、造口渗漏次数更高,自我护理能力测定(ESCA)量表评分更低(P<0.05)。多因素Logistic回归分析结果显示,体质指数、造口渗漏次数、ESCA量表评分是根治性膀胱切除术后患者发生PMASD的影响因素(P<0.05)。ROC曲线结果显示,体质指数、造口渗漏次数、ESCA量表评分预测根治性膀胱切除术后患者发生PMASD的曲线下面积分别为0.921、0.622、0.855,体质指数和ESCA量表评分的曲线下面积大于造口渗漏次数的曲线下面积(P<0.05)。结论根治性膀胱切除术后患者PMASD的发生率较高,体质指数、造口渗漏次数、ESCA量表评分为根治性膀胱切除术后患者发生PMASD的影响因素,应针对肥胖及自护能力较弱的根治性膀胱切除术后患者采取针对性措施,以降低PMASD的发生率。

梨采后黑皮病的发生机制、影响因素和调控措施研究进展

梨是全球大宗水果之一,产后大部分进入中长期贮藏,满足不同时间和空间的市场供应,因此采后贮运是梨果保质增值和产业健康发展的重要环节。然而,梨果采后果皮表面易发生黑皮病。作为一种生理性病害,黑皮病常出现在贮藏后期及出库后的货架期。因发病原因复杂,目前没有有效的保鲜措施,黑皮病成为梨果实采后贮运亟待解决的主要问题。文章阐述了近年来有关梨采后黑皮病发生的可能性机制,分析探讨了品种、产地、成熟度、贮藏环境等因素对梨采后黑皮病发生率的影响,同时从温度调控、气体调控、激素调控等方面总结了防控梨黑皮病发生的保鲜措施,并对今后梨果黑皮病的进一步研究提出了建议,以期为梨果采后贮运保鲜的理论研究和技术开发提供参考。