This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC...This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.展开更多
Jinhehuan (sponge tree) is a kind of rare plant growing on the Tianshan mountain in the Xinjiang region of China, the red pigment of which is good for cooling and activating the blood, having antipyretic, detoxified, ...Jinhehuan (sponge tree) is a kind of rare plant growing on the Tianshan mountain in the Xinjiang region of China, the red pigment of which is good for cooling and activating the blood, having antipyretic, detoxified, antibacterial and antiphlogistic functions. Experts at the Research Institute of Phytology under the Academia Sinica have found that this kind of pigment has a strong展开更多
Jinhehuan(sponge tree)is a kind ofrare plant growing on the Tianshanmountain in the Xinjiang region of China,the red pigment of which is good forcooling and activating the blood, havingantipyretic, detoxified, antibac...Jinhehuan(sponge tree)is a kind ofrare plant growing on the Tianshanmountain in the Xinjiang region of China,the red pigment of which is good forcooling and activating the blood, havingantipyretic, detoxified, antibacterial andantiphlogistic functions. Experts at theResearch Institute of Phytology under theAcademia Sinica have found that thiskind of pigment has a strong展开更多
As far as Chinese herbal medicine resources are concerned,China is one of the countries with the richest biodiversity in the world.As for the future development direction of Chinese skin care products,the development ...As far as Chinese herbal medicine resources are concerned,China is one of the countries with the richest biodiversity in the world.As for the future development direction of Chinese skin care products,the development of special Chinese herbal skin care products is an important issue.The technical route for the research and development(R&D)of Chinese herbal-medicine skin care products should focus on“combining ancient and modern,combining Chinese and Western”,that is to say combining the ancient knowledge and wisdom with modern science and technology,and combining Chinese traditional therapy with western pharmacology.This article will elaborate the five stages of developing innovative Chinese herbal skin care products:pondering and thinking stage;extraction and purification stage;experimental evaluation stage;quality index establishment stage;product development stage.The key points of these five stages can provide a reference for the cosmetics R&D personnel.This project will be beneficial to the development of Chinese herbal-medicine skin care products and the formulation of an overall effective development strategy,which is helpful to actively respond to new challenges and continuously improve product competitiveness,so as to vigorously build a world-renowned high-end cosmetic brand in China,and strive to cultivate a world-class cosmetics talent team.展开更多
Eight types of spongy sheet were prepared by freeze-drying aqueous solutions of hyaluronic acid (HA) and poly(γ-glutamic acid) (PGA) with or without bioactive components including vitamin C derivative (VC), glucosylc...Eight types of spongy sheet were prepared by freeze-drying aqueous solutions of hyaluronic acid (HA) and poly(γ-glutamic acid) (PGA) with or without bioactive components including vitamin C derivative (VC), glucosylceramide (GC), and epidermal growth factor (EGF). Spongy sheets were categorized into the following groups: Group I (HA/PGA), Group II (HA/PGA + VC), Group III (HA/PGA + GC), Group IV (HA/PGA + VC, GC), Group V (HA/PGA + EGF), Group VI (HA/PGA + VC, EGF), Group VII (HA/PGA + GC, EGF), and Group VIII (HA/PGA + VC, GC, EGF). In the first experiment, we examined fibroblast proliferation in conditioned medium that had been prepared by immersing each spongy sheet in a conventional culture medium. EGF-incorporating spongy sheets (Groups V-VIII) enhanced fibroblast proliferation more than EGF-free spongy sheets (Groups I-IV). In the second experiment, cytokine production by fibroblasts was evaluated using a wound surface model. This involved elevation of fibroblasts-incorporating collagen gel sheets to the air-liquid interface, on which a spongy sheet (Groups I, IV, V and VIII) was placed and cultured for 1 week. EGF-incorporating spongy sheets (Groups V and VIII) enhanced the production of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) by fibroblasts more than EGF-free spongy sheets (Groups I and IV). The effect of these four types of spongy sheet on wounds was investigated in animal experiments. Chemical peel was performed by contacting 50% trichloroacetic acid (TCA) on the dorsal region of mice, after which a spongy sheet was placed, and the wound condition was then observed in a two-week period. Angiogenesis was facilitated to a greater degree in Group VIII compared with Groups I, IV and V. This finding indicates that Group VIII spongy sheet is a promising aid for skin recovery after chemical peel.展开更多
文摘This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.
文摘Jinhehuan (sponge tree) is a kind of rare plant growing on the Tianshan mountain in the Xinjiang region of China, the red pigment of which is good for cooling and activating the blood, having antipyretic, detoxified, antibacterial and antiphlogistic functions. Experts at the Research Institute of Phytology under the Academia Sinica have found that this kind of pigment has a strong
文摘Jinhehuan(sponge tree)is a kind ofrare plant growing on the Tianshanmountain in the Xinjiang region of China,the red pigment of which is good forcooling and activating the blood, havingantipyretic, detoxified, antibacterial andantiphlogistic functions. Experts at theResearch Institute of Phytology under theAcademia Sinica have found that thiskind of pigment has a strong
文摘As far as Chinese herbal medicine resources are concerned,China is one of the countries with the richest biodiversity in the world.As for the future development direction of Chinese skin care products,the development of special Chinese herbal skin care products is an important issue.The technical route for the research and development(R&D)of Chinese herbal-medicine skin care products should focus on“combining ancient and modern,combining Chinese and Western”,that is to say combining the ancient knowledge and wisdom with modern science and technology,and combining Chinese traditional therapy with western pharmacology.This article will elaborate the five stages of developing innovative Chinese herbal skin care products:pondering and thinking stage;extraction and purification stage;experimental evaluation stage;quality index establishment stage;product development stage.The key points of these five stages can provide a reference for the cosmetics R&D personnel.This project will be beneficial to the development of Chinese herbal-medicine skin care products and the formulation of an overall effective development strategy,which is helpful to actively respond to new challenges and continuously improve product competitiveness,so as to vigorously build a world-renowned high-end cosmetic brand in China,and strive to cultivate a world-class cosmetics talent team.
文摘Eight types of spongy sheet were prepared by freeze-drying aqueous solutions of hyaluronic acid (HA) and poly(γ-glutamic acid) (PGA) with or without bioactive components including vitamin C derivative (VC), glucosylceramide (GC), and epidermal growth factor (EGF). Spongy sheets were categorized into the following groups: Group I (HA/PGA), Group II (HA/PGA + VC), Group III (HA/PGA + GC), Group IV (HA/PGA + VC, GC), Group V (HA/PGA + EGF), Group VI (HA/PGA + VC, EGF), Group VII (HA/PGA + GC, EGF), and Group VIII (HA/PGA + VC, GC, EGF). In the first experiment, we examined fibroblast proliferation in conditioned medium that had been prepared by immersing each spongy sheet in a conventional culture medium. EGF-incorporating spongy sheets (Groups V-VIII) enhanced fibroblast proliferation more than EGF-free spongy sheets (Groups I-IV). In the second experiment, cytokine production by fibroblasts was evaluated using a wound surface model. This involved elevation of fibroblasts-incorporating collagen gel sheets to the air-liquid interface, on which a spongy sheet (Groups I, IV, V and VIII) was placed and cultured for 1 week. EGF-incorporating spongy sheets (Groups V and VIII) enhanced the production of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) by fibroblasts more than EGF-free spongy sheets (Groups I and IV). The effect of these four types of spongy sheet on wounds was investigated in animal experiments. Chemical peel was performed by contacting 50% trichloroacetic acid (TCA) on the dorsal region of mice, after which a spongy sheet was placed, and the wound condition was then observed in a two-week period. Angiogenesis was facilitated to a greater degree in Group VIII compared with Groups I, IV and V. This finding indicates that Group VIII spongy sheet is a promising aid for skin recovery after chemical peel.
