Dear Editor,The skin barrier can be impaired by acute skin wounds,which may lead to a series of complications.It is essential to accelerate wound healing and rapidly restore the structural integrity and functionality ...Dear Editor,The skin barrier can be impaired by acute skin wounds,which may lead to a series of complications.It is essential to accelerate wound healing and rapidly restore the structural integrity and functionality of skin.One of the promising bioactive agents is human salivary histatin 1(Hst1),a 38-amino acid histidine-rich peptide that functions to maintain the homeostasis of oral mucosa with a cellular mechanism of promoting the adhesion,spreading,migration of epithelial cells and thus re-epithelialization[1].In recent years,Hst1 has been shown to be effective against various skin-related cell types,such as fibroblasts,myo-fibroblasts,keratinocytes and endothelial cells.In our latest in-vivo study,Hst1 not only promotes angiogenesis,re-epithelialization and collagen production,but also suppresses inflammation,thereby significantly accelerating acute skin wound healing in mice[2].All these studies show that Hst1 is a potent bioactive agent for accelerating acute skin wound healing.展开更多
The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achie...The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achieve rapid healing at a reasonable cost, with minimal inconvenience to the patient. Microcurrent dressing, a novel wound dressing with inherent electric activity, can generate low-level microcurrents at the device-wound contact surface in the presence of moisture and can provide an advanced wound healing solution for managing wounds. This article offers a review of the effects and mechanisms of the microcurrent dressing on the healing of skin wounds.展开更多
Tissue engineering has become a hot issue for skin wound healing because it can be used as an alternative treatment to traditional grafts.Nanofibrous films have been widely used due to their excellent properties.In th...Tissue engineering has become a hot issue for skin wound healing because it can be used as an alternative treatment to traditional grafts.Nanofibrous films have been widely used due to their excellent properties.In this work,an organic/inorganic composite poly(arylene sulfide sulfone)/ZnO/graphene oxide(PASS/ZnO/GO)nanofibrous film was fabricated with the ZnO nano-particles blending in an electrospun solution and post-treated with the GO deposition.The optimal PASS/ZnO/GO nanofibrous film was prepared by 2%ZnO nanoparticles,3.0g/mL PASS electrospun solution,and 1%GO dispersion solution.The morphology,hydrophilicity,mechanical property,and cytotoxicity of the PASS/ZnO/GO nanofibrous film were character-ized by using scanning electron microscopy,transmission electron microscope,water contact angle,tensile testing,and a Live/Dead cell staining kit.It is founded that the PASS/ZnO/GO nanofibrous film has outstanding mechanical properties and no cytotoxicity.Furthermore,the PASS/ZnO/GO nanofibrous film exhibits excellent antibacterial activity to both Escherichia coli and Staphylococcus aureus.Above all,this high mechanical property in the non-toxic and antibacterial nanofibrous film will have excellent application prospects in skin wound dressing.展开更多
Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injur...Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injuries,encompassing collagen deposition,angiogenesis,inflammation,and re-epithelialization.Employing electrical stimulation therapy replicates and enhances the effects of endogenous wound electric fields by applying an external electric field to the wound site,thereby promoting skin wound healing.In this study,we developed a self-powered repetitive mechanical impacts-electrical stimulation(RMI-ES)system utilizing a BaTiO_(3)/polydimethylsiloxane(PDMS)piezoelectric composite film.Compared to conventional electrical stimulation devices,the fabricated piezoelectric composite film efficiently harvests energy from the pressure applied by the stimulation device and the tensile force occurring during natural rat activities.The results demonstrated that piezoelectric stimulation generated by the composite membrane expedited the cell cycle,promoting fibroblast proliferation.Additionally,piezoelectric stimulation induced favorable changes in fibroblast gene expression,including increased expression of transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),collagen 1,collagen 3,vascular endothelial growth factor(VEGF),and alpha-smooth muscle actin(α-SMA),while reducing interleukin-6(IL-6)expression.Transcriptome analysis revealed that piezoelectric stimulation may induce fibroblast migration,proliferation,and collagen expression by influencing PI3K/AKT serine/threonine kinase(AKT)pathways.Further confirmation through the addition of the PI3K inhibitor LY294002 validated that piezoelectric stimulation can regulate the repair process after skin injury through the pathway.Importantly,in vivo results demonstrated that the electric field at the wound site effectively promoted wound healing,reduced inflammation,and stimulated collagen deposition and neovascularization.This study emphasizes the role of the piezoelectric membrane as an effective,safe,and battery-free electrical stimulator crucial for skin wound healing.展开更多
A novel sprayable adhesive is established(ZnMet-PF127)by the combination of a thermosensitive hydrogel(Pluronic F127,PF127)and a coordination complex of zinc and metformin(ZnMet).Here we demonstrate that ZnMet-PF127 p...A novel sprayable adhesive is established(ZnMet-PF127)by the combination of a thermosensitive hydrogel(Pluronic F127,PF127)and a coordination complex of zinc and metformin(ZnMet).Here we demonstrate that ZnMet-PF127 potently promotes the healing of traumatic skin defect and burn skin injury by promoting cell proliferation,angiogenesis,collagen formation.Furthermore,we find that ZnMet could inhibit reactive oxygen species(ROS)production through activation of autophagy,thereby protecting cell from oxidative stress induced damage and promoting healing of skin wound.ZnMet complex exerts better effects on promoting skin wound healing than ZnCl2 or metformin alone.ZnMet complex also displays excellent antibacterial activity against Staphylococcus aureus or Escherichia coli,which could reduce the incidence of skin wound infections.Collectively,we demonstrate that sprayable PF127 could be used as a new drug delivery system for treatment of skin injury.The advantages of this sprayable system are obvious:(1)It is convenient to use;(2)The hydrogel can cover irregular skin defect sites evenly in a liquid state.In combination with this system,we establish a novel sprayable adhesive(ZnMet-PF127)and demonstrate that it is a potential clinical treatment for traumatic skin defect and burn skin injury.展开更多
The skin plays a fundamental role in regulating the body's internal balance and protecting against external traumas.A broad variety of environmental risk factors frequently result in acute skin wounds,whose inappr...The skin plays a fundamental role in regulating the body's internal balance and protecting against external traumas.A broad variety of environmental risk factors frequently result in acute skin wounds,whose inappropriate treatments would lead to chronic skin wounds that are difficult to heal.Traditional dressings have been widely used to repair chronic skin wounds,however their drawbacks such as insufficient hemostatic efficacy and non-moist environment have severely limited their clinical applications.As the principal component of skin,collagen has always been a research hotspot in the field of chronic skin wounds due to its advantages of low antigenicity,high biocompatibility and superior bioactivity.Collagen-based dressings have been increasingly developed to heal the chronic wounds during the past decades,arising from their capability in decreasing protein and electrolyte losses in wound exudate,preventing bacterial contamination,permitting less painful dressing changes,and improving the healing quality.This review overviews recent progress of collagen dressings for chronic skin wound healing.Various commonly used wound dressings for wound management have been first introduced.Collagen wound dressings have been categorized as films,sponges,hydrogels,nanofibers,and powders,and their efficacy has been compared.The critical functions of collagen dressings in wound healing,such as stopping bleeding,shortening inflammation,promoting angiogenesis,and stimulating tissue regeneration have been elaborated.The clinical applications of collagen dressings to repair different types of chronic wounds have been thoroughly summarized.A comprehensive list of commercialized collagen dressings has been updated,and an outlook of collagen dressings have been finally speculated.展开更多
With the increasing prevalence of drug-resistant bacterial infections and the slow healing of chronically infected wounds,the development of new antibacterial and accelerated wound healing dressings has become a serio...With the increasing prevalence of drug-resistant bacterial infections and the slow healing of chronically infected wounds,the development of new antibacterial and accelerated wound healing dressings has become a serious challenge.In order to solve this problem,we developed photo-crosslinked multifunctional antibacterial adhesive anti-oxidant hemostatic hydrogel dressings based on polyethylene glycol monomethyl ether modified glycidyl methacrylate functionalized chitosan(CSG-PEG),methacrylamide dopamine(DMA)and zinc ion for disinfection of drug-resistant bacteria and promoting wound healing.The mechanical properties,rheological properties and morphology of hydrogels were characterized,and the biocompatibility of these hydrogels was studied through cell compatibility and blood compatibility tests.These hydrogels were tested for the in vitro blood-clotting ability of whole blood and showed good hemostatic ability in the mouse liver hemorrhage model and the mouse-tail amputation model.In addition,it has been confirmed that the multifunctional hydrogels have good inherent antibacterial properties against Methicillin-resistant Staphylococcus aureus(MRSA).In the full-thickness skin defect model infected with MRSA,the wound closure ratio,thickness of granulation tissue,number of collagen deposition,regeneration of blood vessels and hair follicles were measured.The inflammation-related cytokines(CD68)and angiogenesis-related cytokines(CD31)expressed during skin regeneration were studied.All results indicate that these multifunctional antibacterial adhesive hemostatic hydrogels have better healing effects than commercially available Tegaderm™Film,revealing that they have become promising alternative in the healing of infected wounds.展开更多
An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in m...An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in many countries.However,whether they have reliable effects remains controversial,and their application for skin wound treatment needs to be further standardized and optimized in terms of socio-economic considerations.Thus,the Chinese Burn Association developed this guideline indicating efficacy,application details,adverse reactions and precautions of five clinically common topical growth factors using the Grading of Recommendations Assessment Development and Evaluation method to promote the rational application of topical exogenous growth factors in skin wounds and to benefit more patients.展开更多
Background:Promoting wound healing is crucial to restore the vital barrier function of injured skin.Growth factor products including epidermal growth factor(EGF),fibroblast growth factor(FGF)and granulocyte-macrophage...Background:Promoting wound healing is crucial to restore the vital barrier function of injured skin.Growth factor products including epidermal growth factor(EGF),fibroblast growth factor(FGF)and granulocyte-macrophage colony stimulating factor(GM-CSF)have been used for decades although no systematic evaluation exists regarding their effectiveness and safety issues in treating acute skin wounds.This has resulted in a lack of guidelines and standards for proper application regimes.Therefore,this systematic review and meta-analysis was performed to critically evaluate the effectiveness and safety of these growth factors on skin acute wounds and provide guidelines for application regimes.Methods:We searched PubMed/Medline(1980-2020),Cochrane Library(1980-2020),Cochrane CENTRAL(from establishment to 2020),ClinicalTrials.gov(from establishment to 2020),Chinese Journal Full-text Database(CNKI,1994-2020),China Biology Medicine disc(CBM,1978-2019),Chinese Scientific Journal Database(VIP,1989-2020)andWanfang Database(WFDATA,1980-2019).Randomized controlled trials(RCTs),quasi-RCTs and controlled clinical trials treating patients with acute skin wounds from various causes and with those available growth factors were included.Results:A total of 7573 papers were identified through database searching;229 papers including 281 studies were kept after final screening.Administering growth factors significantly short-ened the healing time of acute skin wounds,including superficial burn injuries[mean differ-ence(MD)=−3.02;95%confidence interval(CI):−3.31∼−2.74;p<0.00001],deep burn injuries(MD=−5.63;95%CI:−7.10∼−4.17;p<0.00001),traumata and surgical wounds(MD=−4.50;95%CI:−5.55∼−3.44;p<0.00001).Growth factors increased the healing rate of acute skin wounds and decreased scar scores.The incidence of adverse reactions was lower in the growth factor treatment group than in the non-growth factor group.Conclusions:The studied growth factors not only are effective and safe for managing acute skin wounds,but also accelerate their healing with no severe adverse reactions.展开更多
Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization.Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation.A...Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization.Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation.Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing,effective management of large chronic skin wounds remains a clinical challenge.Keratinocytes are critical to re-epithelialization and wound healing.Here,we investigated whether exogenous keratinocytes,in combination with a citrate-based scaffold,enhanced skin wound healing.We first established reversibly immortalized mouse keratinocytes(iKera),and confirmed that the iKera cells expressed keratinocyte markers,and were responsive to UVB treatment,and were non-tumorigenic.In a proof-of-principle experiment,we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone,in a mouse skin wound model.Thus,these results demonstrate that iKera cells may serve as a valuable skin epithelial source when,combining with appropriate biocompatible scaffolds,to investigate cutaneous wound healing and skin regeneration.展开更多
The manuscript describes the efficacy of a new skin closure system(Zip Line?) for wound closure after pacemaker/implantable cardioverter defibrillator surgery. The system is particularly useful when wound healingis di...The manuscript describes the efficacy of a new skin closure system(Zip Line?) for wound closure after pacemaker/implantable cardioverter defibrillator surgery. The system is particularly useful when wound healingis difficult with traditional methods and in patients at high risk for surgical site infections(SSIs). This skin closure option is easy and quick to apply and remove, and produces excellent cosmetic results. Although it is associated with a minimal expense upcharge, the benefits, including the potential for decrease in SSI, make it attractive and worth considering for skin closure in device patients, particularly those at increased risk of complications.展开更多
Background: Recent research into skin injury and wound healing has focused mainly on post‐trauma hemostasis, infection prevention, dermal regeneration and angiogenesis. However, less attention has been paid to air pe...Background: Recent research into skin injury and wound healing has focused mainly on post‐trauma hemostasis, infection prevention, dermal regeneration and angiogenesis. However, less attention has been paid to air permeability and moisture loss prevention which also play important roles in injury healing. Methods: In the present work, we prepared a hyaluronic acid‐poloxamer (HA‐POL) hydrogel and tested the therapeutic effect of the hydrogel on skin‐wound healing. Results: The HA‐POL hydrogel transformed from sol to gel at 30°C, close to body temperature, and had stable moisturizing properties. HA‐POL hydrogel promoted skin‐wound healing and increased protein accumulation in the wound area. HA‐POL hydrogel allowed greater air permeability than Band‐aid, a typical wound covering. Results from transwell assays showed that the HA‐POL hydrogel effectively isolated skin‐wounds from bacterial invasion. Conclusion: This work demonstrates the advantages of using HA‐POL gel materials in the treatment of cutaneous wounds.展开更多
Background: Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected a...Background: Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing.Methods: Shark skin collagen(SSC) was purified via ethanol de-sugaring and de-pigmentation and adjusted for p H. A shark skin collagen sponge(SSCS) was prepared by freeze-drying. SSCS was attached to an anti-seawater immersion polyurethane(PU) film(SSCS+PU) to compose a new dressing. The biochemical properties of SSC and physicochemical properties of SSCS were assessed by standard methods. The effects of SSCS and SSCS+PU on the healing of seawaterimmersed wounds were studied using a seawater immersion rat model. For the detection of SSCS effects on seawaterimmersed wounds, 12 SD rats, with four wounds created in each rat, were divided into four groups: the 3 rd day group, 5 th day group, 7 th day group and 12 th day group. In each group, six wounds were treated with SSCS, three wounds treated with chitosan served as the positive control, and three wounds treated with gauze served as the negative control. For the detection of the SSCS+PU effects on seawater-immersed wounds, 36 SD rats were divided into three groups: the gauze(GZ)+PU group, chitosan(CS)+PU group and SSCS+PU group, with 12 rats in each group, and two wounds in each rat. The wound sizes were measured to calculate the healing rate, and histomorphology and the immunohistochemistry of the CD31 and TGF-β expression levels in the wounded tissues were measured by standard methods.Results: The results of Ultraviolet-visible(UV-vis) spectrum, Fourier-transform infrared(FTIR) spectrum, circular dichroism(CD) spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), and amino acid composition analyses of SSC demonstrated that SSC is type I collagen. SSCS had a homogeneous porous structure of approximately 200μm, porosity rate of 83.57%±2.64%, water vapor transmission ratio(WVTR) of 4500 g/m2, tensile strength of 1.79±0.41 N/mm, and elongation at break of 4.52%±0.01%. SSCS had significant beneficial effects on seawater-immersed wound healing. On the 3 rd day, the healing rates in the GZ negative control, CS positive control and SSCS rats were 13.94%±5.50%, 29.40%±1.10% and 47.24%±8.40%, respectively. SSCS also enhanced TGF-in the initial stage of the healing period. The SSCS+PU dressing effectively protected woundsβ and CD31 expression from seawater immersion for at least 4 h, and accelerated re-epithelialization, vascularization and granulation formation of seawater-immersed wounds in the earlier stages of wound healing, and as well as significantly promoted wound healing. The SSCS+PU dressing also enhanced expression of TGF-n and gauze dressings.β and CD31. The effects of SSCS and SSCS+PU were superior to those of both the chitosaConclusion: SSCS has significant positive effects on the promotion of seawater-immersed wound healing, and a SSCS+PU dressing effectively prevents seawater immersion, and significantly promotes seawater-immersed wound healing.展开更多
<span style="font-family:Verdana;">Cancer cells can be proliferating in a few months and years</span><span style="font-family:Verdana;">.</span><span style="font-fam...<span style="font-family:Verdana;">Cancer cells can be proliferating in a few months and years</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> It depends </span><span style="font-family:Verdana;">on</span><span style="font-family:Verdana;"> cancer stage. Chemotherapy, immunotherapy and anti-metabolic drugs have been used in order to kill cancer cells and prevent immune system weakly and metastasis. However, such drugs can damage healthy cells too. Natural ways to cancer treatments may help whole body to cancer cells. In this work, it was taking off cancer nodule to skin cancer by surgery and we treat the nodule as wound, using Nanoskin</span><sup><span style="font-family:Verdana;"><sup></sup></span><span style="font-family:Verdana;background-color:#FFFFFF;"><sup><span style="font-family:Verdana, Helvetica, Arial;">®</sup></span></span></sup><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"> advance cell therapy (ACT), natural extra cellular matrix which releases nutrients to the skin cancer. Our result shows that the cancer nodule disappear</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> in few weeks in skin, because of natural membrane treatment. In addition, we obtained complete wound healing due anticancer nutrients (beta-glucan) delivery to skin.</span>展开更多
The use of free grafts to close defects in wounds following surgery has long been utilized in dermatology practice. However, because of the low survival of the grafts, their popularity has dwindled over recent years. ...The use of free grafts to close defects in wounds following surgery has long been utilized in dermatology practice. However, because of the low survival of the grafts, their popularity has dwindled over recent years. The use of techniques such as attaching an “umbilical cord” from the base of the grafts to the underlying deep fascia or cartilage has markedly increased graft survival. In this paper, the scope of free grafts is presented, showing survival of even large grafts if anchored to the deep tissue with multiple “umbilical cord” attachments. The advantages of full-thickness free skin grafts include the ability to close the wound immediately following removal of the tumor, with decreased risk of infection and pain, requiring fewer dressing changes and visits. The technique of close stitching, with interrupted sutures 1 - 2 mm apart, prevents contact of the wound with oxygen from the air, resulting in increased graft survival from revascularization of the free graft. With the added use of extra-strength curcumin gel, perfect regeneration may be achieved. In addition, in most free grafts, it was observed that there was recovery of sensation due to presumed nerve regeneration.展开更多
The issue of skin defects is a major concern of almost every trauma surgeon after surgery. Despite numerous conventional methods and introduction of the reconstruction ladder, managing skin defects is still a challeng...The issue of skin defects is a major concern of almost every trauma surgeon after surgery. Despite numerous conventional methods and introduction of the reconstruction ladder, managing skin defects is still a challenge for the trauma surgeons. In recent years, parallel to the advances in the more conventional methods of skin repair, regenerative medicine has offered new and novel treatments. This article aims to explore these contemporary regenerative solutions as well as to review the conventional methods of treating skin defects.展开更多
基金funded by the National Natural Science Foundation of China(82172223)the National Key Research and Development Plan of China(2017YFC1103301)+1 种基金the Military Medical Innovation Special Projects(18CXZ029)the Key Research and Development Plan of Zhejiang Province(2021C04013).
文摘Dear Editor,The skin barrier can be impaired by acute skin wounds,which may lead to a series of complications.It is essential to accelerate wound healing and rapidly restore the structural integrity and functionality of skin.One of the promising bioactive agents is human salivary histatin 1(Hst1),a 38-amino acid histidine-rich peptide that functions to maintain the homeostasis of oral mucosa with a cellular mechanism of promoting the adhesion,spreading,migration of epithelial cells and thus re-epithelialization[1].In recent years,Hst1 has been shown to be effective against various skin-related cell types,such as fibroblasts,myo-fibroblasts,keratinocytes and endothelial cells.In our latest in-vivo study,Hst1 not only promotes angiogenesis,re-epithelialization and collagen production,but also suppresses inflammation,thereby significantly accelerating acute skin wound healing in mice[2].All these studies show that Hst1 is a potent bioactive agent for accelerating acute skin wound healing.
基金supported by the National Science Foundation of China(81301345)
文摘The variety of wound types has resulted in a wide range of wound dressings, with new products frequently being introduced to target different aspects of the wound healing process. The ideal wound dressing should achieve rapid healing at a reasonable cost, with minimal inconvenience to the patient. Microcurrent dressing, a novel wound dressing with inherent electric activity, can generate low-level microcurrents at the device-wound contact surface in the presence of moisture and can provide an advanced wound healing solution for managing wounds. This article offers a review of the effects and mechanisms of the microcurrent dressing on the healing of skin wounds.
基金This work was supported by the National Natural Science Foundation of China(Project Nos.51573103 and 21274094)the 2019 foundation research fostering project-21,and the postdoctoral fund(2019SCU12007)from Sichuan University,financially supported by the State Key Laboratory of Polymer Materials Engineering(Grant No.sklpme2020-3-02).
文摘Tissue engineering has become a hot issue for skin wound healing because it can be used as an alternative treatment to traditional grafts.Nanofibrous films have been widely used due to their excellent properties.In this work,an organic/inorganic composite poly(arylene sulfide sulfone)/ZnO/graphene oxide(PASS/ZnO/GO)nanofibrous film was fabricated with the ZnO nano-particles blending in an electrospun solution and post-treated with the GO deposition.The optimal PASS/ZnO/GO nanofibrous film was prepared by 2%ZnO nanoparticles,3.0g/mL PASS electrospun solution,and 1%GO dispersion solution.The morphology,hydrophilicity,mechanical property,and cytotoxicity of the PASS/ZnO/GO nanofibrous film were character-ized by using scanning electron microscopy,transmission electron microscope,water contact angle,tensile testing,and a Live/Dead cell staining kit.It is founded that the PASS/ZnO/GO nanofibrous film has outstanding mechanical properties and no cytotoxicity.Furthermore,the PASS/ZnO/GO nanofibrous film exhibits excellent antibacterial activity to both Escherichia coli and Staphylococcus aureus.Above all,this high mechanical property in the non-toxic and antibacterial nanofibrous film will have excellent application prospects in skin wound dressing.
基金supported by the National Natural Science Foundation of China(Nos.31870967 to W.L.and 81701841 to W.B.W.)the National Key R&D Program of China(No.2018YFC1105800 to W.L.)。
文摘Skin wounds are common in accidental injuries,and the intricacies of wound repair are closely linked to endogenous electric fields.Electrical stimulation plays a pivotal role in the restorative processes of skin injuries,encompassing collagen deposition,angiogenesis,inflammation,and re-epithelialization.Employing electrical stimulation therapy replicates and enhances the effects of endogenous wound electric fields by applying an external electric field to the wound site,thereby promoting skin wound healing.In this study,we developed a self-powered repetitive mechanical impacts-electrical stimulation(RMI-ES)system utilizing a BaTiO_(3)/polydimethylsiloxane(PDMS)piezoelectric composite film.Compared to conventional electrical stimulation devices,the fabricated piezoelectric composite film efficiently harvests energy from the pressure applied by the stimulation device and the tensile force occurring during natural rat activities.The results demonstrated that piezoelectric stimulation generated by the composite membrane expedited the cell cycle,promoting fibroblast proliferation.Additionally,piezoelectric stimulation induced favorable changes in fibroblast gene expression,including increased expression of transforming growth factor-β1(TGF-β1),connective tissue growth factor(CTGF),collagen 1,collagen 3,vascular endothelial growth factor(VEGF),and alpha-smooth muscle actin(α-SMA),while reducing interleukin-6(IL-6)expression.Transcriptome analysis revealed that piezoelectric stimulation may induce fibroblast migration,proliferation,and collagen expression by influencing PI3K/AKT serine/threonine kinase(AKT)pathways.Further confirmation through the addition of the PI3K inhibitor LY294002 validated that piezoelectric stimulation can regulate the repair process after skin injury through the pathway.Importantly,in vivo results demonstrated that the electric field at the wound site effectively promoted wound healing,reduced inflammation,and stimulated collagen deposition and neovascularization.This study emphasizes the role of the piezoelectric membrane as an effective,safe,and battery-free electrical stimulator crucial for skin wound healing.
基金supported,in part,by the National Key Research and Development Program of China Grants(2019YFA0906001)National Natural Science Foundation of China Grants(82022047,81972100)Guangdong Provincial Science and Technology Innovation Council Grant(2017B030301018).
文摘A novel sprayable adhesive is established(ZnMet-PF127)by the combination of a thermosensitive hydrogel(Pluronic F127,PF127)and a coordination complex of zinc and metformin(ZnMet).Here we demonstrate that ZnMet-PF127 potently promotes the healing of traumatic skin defect and burn skin injury by promoting cell proliferation,angiogenesis,collagen formation.Furthermore,we find that ZnMet could inhibit reactive oxygen species(ROS)production through activation of autophagy,thereby protecting cell from oxidative stress induced damage and promoting healing of skin wound.ZnMet complex exerts better effects on promoting skin wound healing than ZnCl2 or metformin alone.ZnMet complex also displays excellent antibacterial activity against Staphylococcus aureus or Escherichia coli,which could reduce the incidence of skin wound infections.Collectively,we demonstrate that sprayable PF127 could be used as a new drug delivery system for treatment of skin injury.The advantages of this sprayable system are obvious:(1)It is convenient to use;(2)The hydrogel can cover irregular skin defect sites evenly in a liquid state.In combination with this system,we establish a novel sprayable adhesive(ZnMet-PF127)and demonstrate that it is a potential clinical treatment for traumatic skin defect and burn skin injury.
基金supported by grants from the National Natural Science Foundation of China(grant nos.22074057,21775059 and 22205089).
文摘The skin plays a fundamental role in regulating the body's internal balance and protecting against external traumas.A broad variety of environmental risk factors frequently result in acute skin wounds,whose inappropriate treatments would lead to chronic skin wounds that are difficult to heal.Traditional dressings have been widely used to repair chronic skin wounds,however their drawbacks such as insufficient hemostatic efficacy and non-moist environment have severely limited their clinical applications.As the principal component of skin,collagen has always been a research hotspot in the field of chronic skin wounds due to its advantages of low antigenicity,high biocompatibility and superior bioactivity.Collagen-based dressings have been increasingly developed to heal the chronic wounds during the past decades,arising from their capability in decreasing protein and electrolyte losses in wound exudate,preventing bacterial contamination,permitting less painful dressing changes,and improving the healing quality.This review overviews recent progress of collagen dressings for chronic skin wound healing.Various commonly used wound dressings for wound management have been first introduced.Collagen wound dressings have been categorized as films,sponges,hydrogels,nanofibers,and powders,and their efficacy has been compared.The critical functions of collagen dressings in wound healing,such as stopping bleeding,shortening inflammation,promoting angiogenesis,and stimulating tissue regeneration have been elaborated.The clinical applications of collagen dressings to repair different types of chronic wounds have been thoroughly summarized.A comprehensive list of commercialized collagen dressings has been updated,and an outlook of collagen dressings have been finally speculated.
基金jointly supported by the National Natural Science Foundation of China(grant numbers:51973172,and 51673155)the Natural Science Foundation of Shaanxi Province(No.2020JC-03 and 2019TD-020)+5 种基金State Key Laboratory for Mechanical Behavior of Materials,and the Fundamental Research Funds for the Central Universitiesthe World-Class Universities(Disciplines)and the Characteristic Development Guidance Funds for the Central UniversitiesOpening Project of Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University(No.2019LHM-KFKT008)the Key R&D Program of Shaanxi Province(No.2019ZDLSF02-09-01,2020GXLH-Y-019)Innovation Capability Support Program of Shaanxi Province(Program No.2019GHJD-14,2021TD-40)Scientific Research Program Funded by Shaanxi Provincial Education Department(Program No.18JC027).
文摘With the increasing prevalence of drug-resistant bacterial infections and the slow healing of chronically infected wounds,the development of new antibacterial and accelerated wound healing dressings has become a serious challenge.In order to solve this problem,we developed photo-crosslinked multifunctional antibacterial adhesive anti-oxidant hemostatic hydrogel dressings based on polyethylene glycol monomethyl ether modified glycidyl methacrylate functionalized chitosan(CSG-PEG),methacrylamide dopamine(DMA)and zinc ion for disinfection of drug-resistant bacteria and promoting wound healing.The mechanical properties,rheological properties and morphology of hydrogels were characterized,and the biocompatibility of these hydrogels was studied through cell compatibility and blood compatibility tests.These hydrogels were tested for the in vitro blood-clotting ability of whole blood and showed good hemostatic ability in the mouse liver hemorrhage model and the mouse-tail amputation model.In addition,it has been confirmed that the multifunctional hydrogels have good inherent antibacterial properties against Methicillin-resistant Staphylococcus aureus(MRSA).In the full-thickness skin defect model infected with MRSA,the wound closure ratio,thickness of granulation tissue,number of collagen deposition,regeneration of blood vessels and hair follicles were measured.The inflammation-related cytokines(CD68)and angiogenesis-related cytokines(CD31)expressed during skin regeneration were studied.All results indicate that these multifunctional antibacterial adhesive hemostatic hydrogels have better healing effects than commercially available Tegaderm™Film,revealing that they have become promising alternative in the healing of infected wounds.
文摘An increased number of patients with skin wounds have been witnessed in the past decades.Among the various kinds of treatments for skin wounds,topical exogenous growth factors are indispensable and have been used in many countries.However,whether they have reliable effects remains controversial,and their application for skin wound treatment needs to be further standardized and optimized in terms of socio-economic considerations.Thus,the Chinese Burn Association developed this guideline indicating efficacy,application details,adverse reactions and precautions of five clinically common topical growth factors using the Grading of Recommendations Assessment Development and Evaluation method to promote the rational application of topical exogenous growth factors in skin wounds and to benefit more patients.
文摘Background:Promoting wound healing is crucial to restore the vital barrier function of injured skin.Growth factor products including epidermal growth factor(EGF),fibroblast growth factor(FGF)and granulocyte-macrophage colony stimulating factor(GM-CSF)have been used for decades although no systematic evaluation exists regarding their effectiveness and safety issues in treating acute skin wounds.This has resulted in a lack of guidelines and standards for proper application regimes.Therefore,this systematic review and meta-analysis was performed to critically evaluate the effectiveness and safety of these growth factors on skin acute wounds and provide guidelines for application regimes.Methods:We searched PubMed/Medline(1980-2020),Cochrane Library(1980-2020),Cochrane CENTRAL(from establishment to 2020),ClinicalTrials.gov(from establishment to 2020),Chinese Journal Full-text Database(CNKI,1994-2020),China Biology Medicine disc(CBM,1978-2019),Chinese Scientific Journal Database(VIP,1989-2020)andWanfang Database(WFDATA,1980-2019).Randomized controlled trials(RCTs),quasi-RCTs and controlled clinical trials treating patients with acute skin wounds from various causes and with those available growth factors were included.Results:A total of 7573 papers were identified through database searching;229 papers including 281 studies were kept after final screening.Administering growth factors significantly short-ened the healing time of acute skin wounds,including superficial burn injuries[mean differ-ence(MD)=−3.02;95%confidence interval(CI):−3.31∼−2.74;p<0.00001],deep burn injuries(MD=−5.63;95%CI:−7.10∼−4.17;p<0.00001),traumata and surgical wounds(MD=−4.50;95%CI:−5.55∼−3.44;p<0.00001).Growth factors increased the healing rate of acute skin wounds and decreased scar scores.The incidence of adverse reactions was lower in the growth factor treatment group than in the non-growth factor group.Conclusions:The studied growth factors not only are effective and safe for managing acute skin wounds,but also accelerate their healing with no severe adverse reactions.
基金The reported study was supported in part by research grants from the 2019 Chongqing Support Program for Entrepreneurship and Innovation(No.cx2019113)(JF)the 2019 Science and Technology Research Plan Project of Chongqing Education Commission(KJQN201900410)(JF)+9 种基金the 2019 Youth Innovative Talent Training Program of Chongqing Education Commission(No.CY200409)(JF)the 2019 Funding for Postdoctoral Research(Chongqing Human Resources and Social Security Bureau No.298)(JF)and the National Key Research and Development Program of China(2016YFC1000803)RRR,TCH and GAA were partially funded by the National Institutes of Health(DE030480)WW was supported by the Medical Scientist Training Program of the National Institutes of Health(T32 GM007281)This project was also supported in part by The University of Chicago Cancer Center Support Grant(P30CA014599)the National Center for Advancing Translational Sciences of the National Institutes of Health through Grant Number UL1 TR000430TCH was also supported by the Mabel Green Myers Research Endowment Fund,The University of Chicago Orthopaedics Alumni Fund,and The University of Chicago SHOCK Fund.Funding sources were not involved in the study designin the collection,analysis and/or interpretation of datain the writing of the reportor in the decision to submit the paper for publication.
文摘Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization.Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation.Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing,effective management of large chronic skin wounds remains a clinical challenge.Keratinocytes are critical to re-epithelialization and wound healing.Here,we investigated whether exogenous keratinocytes,in combination with a citrate-based scaffold,enhanced skin wound healing.We first established reversibly immortalized mouse keratinocytes(iKera),and confirmed that the iKera cells expressed keratinocyte markers,and were responsive to UVB treatment,and were non-tumorigenic.In a proof-of-principle experiment,we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone,in a mouse skin wound model.Thus,these results demonstrate that iKera cells may serve as a valuable skin epithelial source when,combining with appropriate biocompatible scaffolds,to investigate cutaneous wound healing and skin regeneration.
文摘The manuscript describes the efficacy of a new skin closure system(Zip Line?) for wound closure after pacemaker/implantable cardioverter defibrillator surgery. The system is particularly useful when wound healingis difficult with traditional methods and in patients at high risk for surgical site infections(SSIs). This skin closure option is easy and quick to apply and remove, and produces excellent cosmetic results. Although it is associated with a minimal expense upcharge, the benefits, including the potential for decrease in SSI, make it attractive and worth considering for skin closure in device patients, particularly those at increased risk of complications.
基金Beijing Municipal Natural Science Foundation, Grant/Award Number: 7142131
文摘Background: Recent research into skin injury and wound healing has focused mainly on post‐trauma hemostasis, infection prevention, dermal regeneration and angiogenesis. However, less attention has been paid to air permeability and moisture loss prevention which also play important roles in injury healing. Methods: In the present work, we prepared a hyaluronic acid‐poloxamer (HA‐POL) hydrogel and tested the therapeutic effect of the hydrogel on skin‐wound healing. Results: The HA‐POL hydrogel transformed from sol to gel at 30°C, close to body temperature, and had stable moisturizing properties. HA‐POL hydrogel promoted skin‐wound healing and increased protein accumulation in the wound area. HA‐POL hydrogel allowed greater air permeability than Band‐aid, a typical wound covering. Results from transwell assays showed that the HA‐POL hydrogel effectively isolated skin‐wounds from bacterial invasion. Conclusion: This work demonstrates the advantages of using HA‐POL gel materials in the treatment of cutaneous wounds.
基金supported by a Major Project of the Ministry of National Science and Technology of China(Grant No.2014ZX09J14103-09C).
文摘Background: Wounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing.Methods: Shark skin collagen(SSC) was purified via ethanol de-sugaring and de-pigmentation and adjusted for p H. A shark skin collagen sponge(SSCS) was prepared by freeze-drying. SSCS was attached to an anti-seawater immersion polyurethane(PU) film(SSCS+PU) to compose a new dressing. The biochemical properties of SSC and physicochemical properties of SSCS were assessed by standard methods. The effects of SSCS and SSCS+PU on the healing of seawaterimmersed wounds were studied using a seawater immersion rat model. For the detection of SSCS effects on seawaterimmersed wounds, 12 SD rats, with four wounds created in each rat, were divided into four groups: the 3 rd day group, 5 th day group, 7 th day group and 12 th day group. In each group, six wounds were treated with SSCS, three wounds treated with chitosan served as the positive control, and three wounds treated with gauze served as the negative control. For the detection of the SSCS+PU effects on seawater-immersed wounds, 36 SD rats were divided into three groups: the gauze(GZ)+PU group, chitosan(CS)+PU group and SSCS+PU group, with 12 rats in each group, and two wounds in each rat. The wound sizes were measured to calculate the healing rate, and histomorphology and the immunohistochemistry of the CD31 and TGF-β expression levels in the wounded tissues were measured by standard methods.Results: The results of Ultraviolet-visible(UV-vis) spectrum, Fourier-transform infrared(FTIR) spectrum, circular dichroism(CD) spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), and amino acid composition analyses of SSC demonstrated that SSC is type I collagen. SSCS had a homogeneous porous structure of approximately 200μm, porosity rate of 83.57%±2.64%, water vapor transmission ratio(WVTR) of 4500 g/m2, tensile strength of 1.79±0.41 N/mm, and elongation at break of 4.52%±0.01%. SSCS had significant beneficial effects on seawater-immersed wound healing. On the 3 rd day, the healing rates in the GZ negative control, CS positive control and SSCS rats were 13.94%±5.50%, 29.40%±1.10% and 47.24%±8.40%, respectively. SSCS also enhanced TGF-in the initial stage of the healing period. The SSCS+PU dressing effectively protected woundsβ and CD31 expression from seawater immersion for at least 4 h, and accelerated re-epithelialization, vascularization and granulation formation of seawater-immersed wounds in the earlier stages of wound healing, and as well as significantly promoted wound healing. The SSCS+PU dressing also enhanced expression of TGF-n and gauze dressings.β and CD31. The effects of SSCS and SSCS+PU were superior to those of both the chitosaConclusion: SSCS has significant positive effects on the promotion of seawater-immersed wound healing, and a SSCS+PU dressing effectively prevents seawater immersion, and significantly promotes seawater-immersed wound healing.
文摘<span style="font-family:Verdana;">Cancer cells can be proliferating in a few months and years</span><span style="font-family:Verdana;">.</span><span style="font-family:Verdana;"> It depends </span><span style="font-family:Verdana;">on</span><span style="font-family:Verdana;"> cancer stage. Chemotherapy, immunotherapy and anti-metabolic drugs have been used in order to kill cancer cells and prevent immune system weakly and metastasis. However, such drugs can damage healthy cells too. Natural ways to cancer treatments may help whole body to cancer cells. In this work, it was taking off cancer nodule to skin cancer by surgery and we treat the nodule as wound, using Nanoskin</span><sup><span style="font-family:Verdana;"><sup></sup></span><span style="font-family:Verdana;background-color:#FFFFFF;"><sup><span style="font-family:Verdana, Helvetica, Arial;">®</sup></span></span></sup><span style="font-family:Verdana;"></span><span style="font-family:Verdana;"> advance cell therapy (ACT), natural extra cellular matrix which releases nutrients to the skin cancer. Our result shows that the cancer nodule disappear</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> in few weeks in skin, because of natural membrane treatment. In addition, we obtained complete wound healing due anticancer nutrients (beta-glucan) delivery to skin.</span>
文摘The use of free grafts to close defects in wounds following surgery has long been utilized in dermatology practice. However, because of the low survival of the grafts, their popularity has dwindled over recent years. The use of techniques such as attaching an “umbilical cord” from the base of the grafts to the underlying deep fascia or cartilage has markedly increased graft survival. In this paper, the scope of free grafts is presented, showing survival of even large grafts if anchored to the deep tissue with multiple “umbilical cord” attachments. The advantages of full-thickness free skin grafts include the ability to close the wound immediately following removal of the tumor, with decreased risk of infection and pain, requiring fewer dressing changes and visits. The technique of close stitching, with interrupted sutures 1 - 2 mm apart, prevents contact of the wound with oxygen from the air, resulting in increased graft survival from revascularization of the free graft. With the added use of extra-strength curcumin gel, perfect regeneration may be achieved. In addition, in most free grafts, it was observed that there was recovery of sensation due to presumed nerve regeneration.
文摘The issue of skin defects is a major concern of almost every trauma surgeon after surgery. Despite numerous conventional methods and introduction of the reconstruction ladder, managing skin defects is still a challenge for the trauma surgeons. In recent years, parallel to the advances in the more conventional methods of skin repair, regenerative medicine has offered new and novel treatments. This article aims to explore these contemporary regenerative solutions as well as to review the conventional methods of treating skin defects.
