Ultrastructural changes in both pathogen and host cells in the interaction between Puccinia striiformis and wheat cultivar （Libellula） with slow-rusting resistance were observed by transmission electron microscopy. ...Ultrastructural changes in both pathogen and host cells in the interaction between Puccinia striiformis and wheat cultivar （Libellula） with slow-rusting resistance were observed by transmission electron microscopy. Observations revealed marked changes in ultrastructure of both pathogen and host cells. In the pathogen respect, there were many vesicles appeared in the intercellular hyphae and gradually fused into bigger vacuoles, a number of fat drops and electron-dense granules accumulated, mitochondria became swollen and some of them degraded into vesicles, and the plasmalemma of intercellular hyphae became dark. In the haustoria, the cytoplasm degraded gradually and developed a vacuole in the center, fat drops increased, the extrahaustorial matrix widened with a great amount of electron-dense fibrillar and granular materials, and most of the haustoria died with in conjunction with the disappearance of fat drops and other organelles. Structural defense of the host, including formation of cell wall apposition, collar and papilla, occurred in the host respect. Host resistance expression and cytological features occurring in the slow-rusting resistance were discussed.展开更多
The ultrastructure of wheat cultivars with slow-rusting resistance expression to the stripe rust, Puccinia striiformis, wasstudied through TEM. The results show that slow-rusting has the same hypersensitive response c...The ultrastructure of wheat cultivars with slow-rusting resistance expression to the stripe rust, Puccinia striiformis, wasstudied through TEM. The results show that slow-rusting has the same hypersensitive response characters with the lowinfection type resistance, but the mesophyll cell necrotized less in number, thereby only partially inhibiting the extensionof rust fungus, and the fungus being inhibited and necrosed slighter in degree. Apart from the occurrence ofhypersensitiveness, the response of the host cells in slow-rusting wheat cultivars to the infection of fungus also producesstructural materials associated with defense reaction, but distinctly less than that in resistant cultivar. Thus, it is suggestedthat the slow-rusting resistance might have a similar mechanism with the low infection type resistance of race specificity,but with lower intensity.展开更多
基金supported by Provincial Key Scientific and Technological Project of Guizhou,China ([2007] 5003)Guizhou Province Scientific and Technological Research,China ([2007] 2051)
文摘Ultrastructural changes in both pathogen and host cells in the interaction between Puccinia striiformis and wheat cultivar （Libellula） with slow-rusting resistance were observed by transmission electron microscopy. Observations revealed marked changes in ultrastructure of both pathogen and host cells. In the pathogen respect, there were many vesicles appeared in the intercellular hyphae and gradually fused into bigger vacuoles, a number of fat drops and electron-dense granules accumulated, mitochondria became swollen and some of them degraded into vesicles, and the plasmalemma of intercellular hyphae became dark. In the haustoria, the cytoplasm degraded gradually and developed a vacuole in the center, fat drops increased, the extrahaustorial matrix widened with a great amount of electron-dense fibrillar and granular materials, and most of the haustoria died with in conjunction with the disappearance of fat drops and other organelles. Structural defense of the host, including formation of cell wall apposition, collar and papilla, occurred in the host respect. Host resistance expression and cytological features occurring in the slow-rusting resistance were discussed.
文摘The ultrastructure of wheat cultivars with slow-rusting resistance expression to the stripe rust, Puccinia striiformis, wasstudied through TEM. The results show that slow-rusting has the same hypersensitive response characters with the lowinfection type resistance, but the mesophyll cell necrotized less in number, thereby only partially inhibiting the extensionof rust fungus, and the fungus being inhibited and necrosed slighter in degree. Apart from the occurrence ofhypersensitiveness, the response of the host cells in slow-rusting wheat cultivars to the infection of fungus also producesstructural materials associated with defense reaction, but distinctly less than that in resistant cultivar. Thus, it is suggestedthat the slow-rusting resistance might have a similar mechanism with the low infection type resistance of race specificity,but with lower intensity.
基金Fok Ying Tung Eduction Foundation(94021)Beijng Municipal Natural Science Foundation(6061003)National Science Found for Distinguished Young Scholars(30425039)
