目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(...目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(Con)、LPCAT1组、LPCAT1+FH535组和FH535组。运用CCK-8法和集落形成试验检测子宫颈癌细胞的增殖。通过伤口愈合试验和Transwell实验检测子宫颈癌细胞的转移、侵袭能力。应用Western blot分析细胞中LPCAT1、β-catenin/Slug信号通路和EMT相关蛋白的表达。结果与Vector组相比,LPCAT1组SiHa细胞的活力、集落数、迁移和侵袭细胞数均显著增加(P<0.05);与sh-NC组相比,sh-LPCAT1组Hela细胞的活力、集落数、迁移和侵袭细胞数均显著降低(P<0.05)。与LPCAT1组相比,LPCAT1+FH535组SiHa细胞中Wnt4(1.18±0.05 vs 0.80±0.06)、β-catenin(1.05±0.08 vs 0.77±0.05)、Slug(1.13±0.06 vs 0.28±0.02)、Cyclin D1(0.99±0.06 vs 0.44±0.02)、N-cadherin(0.91±0.07 vs 0.46±0.03)和vimentin(0.95±0.06 vs 0.49±0.03)表达降低(P<0.05),E-cadherin(0.44±0.03 vs 0.58±0.03)表达增加(P<0.05)。此外,与LPCAT1组相比,LPCAT1+FH535组SiHa细胞的集落数(224±15 vs 146±11)、迁移数(85±3 vs 51±4)和侵袭数(166±10 vs 90±5)均降低(P<0.05)。结论LPCAT1表达增加可能通过激活β-catenin/Slug信号通路促进子宫颈癌的转移和进展,LPCAT1的靶向治疗有望提高子宫颈癌患者的预后。展开更多
The hydrodynamic study of the liquid film around Taylor bubbles in slug flow has great significance for understanding parallel flow and interaction between Taylor bubbles.The prediction models for liquid film thicknes...The hydrodynamic study of the liquid film around Taylor bubbles in slug flow has great significance for understanding parallel flow and interaction between Taylor bubbles.The prediction models for liquid film thickness mainly focus on stagnant flow,and some of them remain inaccurate performance.However,in the industrial process,the slug flow essentially is co-current flow.Therefore,in this paper,the liquid film thickness is studied by theoretical analysis and experimental methods under two conditions of stagnant and co-current flow.Firstly,under the condition of stagnant flow,the present work is based on Batchelor's theory,and modifies Batchelor's liquid film thickness model,which effectively improves its prediction accuracy.Under the condition of co-current flow,the prediction model of average liquid film thickness in slug flow is established by force and motion analysis.Taylor bubble length is introduced into the model as an important parameter.Dynamic experiments were carried out in the pipe with an inner diameter of 20 mm.The liquid film thickness,Taylor bubble velocity and length were measured by distributed ultrasonic sensor and intrusive cross-correlation conductivity sensor.Comparing the predicted value of the model with the measured results,the relative error is controlled within 10%.展开更多
Large vertical pipes are key structures connecting subsea wells to offshore platforms.However,existing studies mainly focus on small vertical pipes.In a vertical acrylic pipe with 80 mm inner diameter and 11 m height,...Large vertical pipes are key structures connecting subsea wells to offshore platforms.However,existing studies mainly focus on small vertical pipes.In a vertical acrylic pipe with 80 mm inner diameter and 11 m height,a high-speed camera was used to visually research the influences of pipe diameters,liquid properties and inlet effect on air-water co-flow characteristic.Different flow regime maps of vertical pipes(diameters are in the range of 50e189 mm)were compared and the critical gas velocity of the transition boundary from bubble to slug flow tended to increase with the increase of diameters at D≥80 mm.Drift-flux models were established in different flow regimes and liquid properties have a significant effect on drift coefficients of bubble flow and slug flow(void fraction a≤0.4).The influence of inlet turbulent effect on the gas-liquid interface distribution gradually weakened and disappeared from the pipe base to 85D,where the flow was fully developed.Slug frequency has a trend of increase first and then decrease with the gas Weber numbers increasing at low liquid superficial velocities(J_(L)≤0.31 m/s).And on the basis of this law,a new slug frequency correlation was proposed.It was found that there was an exponential relationship between the ratio of lengths of Taylor bubble to slug and the void fraction.展开更多
目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-...目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化,另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果:转染pc DNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pc DNA 3.1-Slug的Eca-109中E-钙黏素表达下调,N-钙黏蛋白和波形蛋白表达上调,细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调,N-钙黏蛋白和波形蛋白表达下调,细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关(r=-0.96)。结论:转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力,而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。展开更多
文摘目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(Con)、LPCAT1组、LPCAT1+FH535组和FH535组。运用CCK-8法和集落形成试验检测子宫颈癌细胞的增殖。通过伤口愈合试验和Transwell实验检测子宫颈癌细胞的转移、侵袭能力。应用Western blot分析细胞中LPCAT1、β-catenin/Slug信号通路和EMT相关蛋白的表达。结果与Vector组相比,LPCAT1组SiHa细胞的活力、集落数、迁移和侵袭细胞数均显著增加(P<0.05);与sh-NC组相比,sh-LPCAT1组Hela细胞的活力、集落数、迁移和侵袭细胞数均显著降低(P<0.05)。与LPCAT1组相比,LPCAT1+FH535组SiHa细胞中Wnt4(1.18±0.05 vs 0.80±0.06)、β-catenin(1.05±0.08 vs 0.77±0.05)、Slug(1.13±0.06 vs 0.28±0.02)、Cyclin D1(0.99±0.06 vs 0.44±0.02)、N-cadherin(0.91±0.07 vs 0.46±0.03)和vimentin(0.95±0.06 vs 0.49±0.03)表达降低(P<0.05),E-cadherin(0.44±0.03 vs 0.58±0.03)表达增加(P<0.05)。此外,与LPCAT1组相比,LPCAT1+FH535组SiHa细胞的集落数(224±15 vs 146±11)、迁移数(85±3 vs 51±4)和侵袭数(166±10 vs 90±5)均降低(P<0.05)。结论LPCAT1表达增加可能通过激活β-catenin/Slug信号通路促进子宫颈癌的转移和进展,LPCAT1的靶向治疗有望提高子宫颈癌患者的预后。
基金supported by National Natural Science Foundation of China(42074142,51527805)。
文摘The hydrodynamic study of the liquid film around Taylor bubbles in slug flow has great significance for understanding parallel flow and interaction between Taylor bubbles.The prediction models for liquid film thickness mainly focus on stagnant flow,and some of them remain inaccurate performance.However,in the industrial process,the slug flow essentially is co-current flow.Therefore,in this paper,the liquid film thickness is studied by theoretical analysis and experimental methods under two conditions of stagnant and co-current flow.Firstly,under the condition of stagnant flow,the present work is based on Batchelor's theory,and modifies Batchelor's liquid film thickness model,which effectively improves its prediction accuracy.Under the condition of co-current flow,the prediction model of average liquid film thickness in slug flow is established by force and motion analysis.Taylor bubble length is introduced into the model as an important parameter.Dynamic experiments were carried out in the pipe with an inner diameter of 20 mm.The liquid film thickness,Taylor bubble velocity and length were measured by distributed ultrasonic sensor and intrusive cross-correlation conductivity sensor.Comparing the predicted value of the model with the measured results,the relative error is controlled within 10%.
基金supported by the National Natural Science Foundation of China(No.51888103,No.52076175).
文摘Large vertical pipes are key structures connecting subsea wells to offshore platforms.However,existing studies mainly focus on small vertical pipes.In a vertical acrylic pipe with 80 mm inner diameter and 11 m height,a high-speed camera was used to visually research the influences of pipe diameters,liquid properties and inlet effect on air-water co-flow characteristic.Different flow regime maps of vertical pipes(diameters are in the range of 50e189 mm)were compared and the critical gas velocity of the transition boundary from bubble to slug flow tended to increase with the increase of diameters at D≥80 mm.Drift-flux models were established in different flow regimes and liquid properties have a significant effect on drift coefficients of bubble flow and slug flow(void fraction a≤0.4).The influence of inlet turbulent effect on the gas-liquid interface distribution gradually weakened and disappeared from the pipe base to 85D,where the flow was fully developed.Slug frequency has a trend of increase first and then decrease with the gas Weber numbers increasing at low liquid superficial velocities(J_(L)≤0.31 m/s).And on the basis of this law,a new slug frequency correlation was proposed.It was found that there was an exponential relationship between the ratio of lengths of Taylor bubble to slug and the void fraction.
文摘目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化,另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果:转染pc DNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pc DNA 3.1-Slug的Eca-109中E-钙黏素表达下调,N-钙黏蛋白和波形蛋白表达上调,细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调,N-钙黏蛋白和波形蛋白表达下调,细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关(r=-0.96)。结论:转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力,而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。
