Bone morphogenetic proteins（BMPs） play critical roles in follicle growth and development.BMPs initiate signaling by assembling BMP receptors and activating Smads,which in turn alter expression of target genes.The me...Bone morphogenetic proteins（BMPs） play critical roles in follicle growth and development.BMPs initiate signaling by assembling BMP receptors and activating Smads,which in turn alter expression of target genes.The mechanism underlying the regulation of the expression of BMP receptors and Smads during follicle development in pigs is still unknown.By quantitative real-time PCR,the mRNA expression of BMP receptors and Smads in granulosa cells（GC） was investigated.Cells were obtained from small porcine follicles（SF;3 mm diameter） and dominant follicles（DF;6 mm diameter）;ActR1A and BMPR2 mRNA levels in DF were significantly higher（P0.05） than that in SF,whereas BMPR1B,Smad4 and Smad7 expression tended to decrease（P0.05）.The levels of BMPR1A,ActR2,Smad1,Smad5,and Smad8 mRNA did not differ between DFs and SFs.To investigate the effect of LH on BMP receptors in GC,cells obtained from porcine DFs were cultured in medium supplemented with different doses of luteinizing hormone（LH）.High doses of LH（4 IU mL-1） significantly decreased the concentration of estradiol（E2） and progesterone（P4） in medium and the expression of Cyp19a1（P450 aromatase,P450arom） and Cyp11a1（cholesterol side-chain cleavage enzyme P450,P450scc）,while significantly increased viable cell numbers and up-regulated expression of cyclin dependent kinase-4（CDK4） and cyclin D2.However,LH had no effect on the expression of BMP receptor genes.Thus,the present study indicates that the expression of members of the BMP signaling pathway in porcine GC is regulated during follicle development and the expression of BMP receptors are not regulated by LH in porcine GCs.展开更多
目的:通过观察加味麻杏石甘汤对放射后肺泡Ⅱ型上皮细胞(AECⅡ)转化生长因子-β1(TGF-β1)m RNA、Smads表达的差异,探讨该方抗放射性肺损伤的分子机制。方法:清洁级SD大鼠18只,加味麻杏石甘汤灌胃给药3d,末次给药后1-2h采血,制备含药血...目的:通过观察加味麻杏石甘汤对放射后肺泡Ⅱ型上皮细胞(AECⅡ)转化生长因子-β1(TGF-β1)m RNA、Smads表达的差异,探讨该方抗放射性肺损伤的分子机制。方法:清洁级SD大鼠18只,加味麻杏石甘汤灌胃给药3d,末次给药后1-2h采血,制备含药血清。用3.64Gy/min,总剂量8Gy的X线照射小鼠AECⅡ细胞,建立放射性损伤的AECⅡ细胞模型。RT-PCR法检测AECⅡTGF-β1、Smads m RNA水平;Western blot法检测AECⅡSmads蛋白的表达水平。结果:与正常大鼠血清组比较,含药血清组在干预48h后,AECⅡ细胞TGF-β1 m RNA及Smad2 m RNA表达降低(P<0.05),Smad6、Smad7 m RNA表达增高;10%、15%含药血清组p-Smad2、p-Smad3蛋白表达下调(P<0.05),Smad6和Smad7蛋白的表达增高(P<0.05)。结论:调控TGF-β1/Smad信号通路可能是加味麻杏石甘汤抑制放射性肺损伤的机制。展开更多
基金supported by the National High Tech-nology Research & Development Program of China(2006AA10Z136)
文摘Bone morphogenetic proteins（BMPs） play critical roles in follicle growth and development.BMPs initiate signaling by assembling BMP receptors and activating Smads,which in turn alter expression of target genes.The mechanism underlying the regulation of the expression of BMP receptors and Smads during follicle development in pigs is still unknown.By quantitative real-time PCR,the mRNA expression of BMP receptors and Smads in granulosa cells（GC） was investigated.Cells were obtained from small porcine follicles（SF;3 mm diameter） and dominant follicles（DF;6 mm diameter）;ActR1A and BMPR2 mRNA levels in DF were significantly higher（P0.05） than that in SF,whereas BMPR1B,Smad4 and Smad7 expression tended to decrease（P0.05）.The levels of BMPR1A,ActR2,Smad1,Smad5,and Smad8 mRNA did not differ between DFs and SFs.To investigate the effect of LH on BMP receptors in GC,cells obtained from porcine DFs were cultured in medium supplemented with different doses of luteinizing hormone（LH）.High doses of LH（4 IU mL-1） significantly decreased the concentration of estradiol（E2） and progesterone（P4） in medium and the expression of Cyp19a1（P450 aromatase,P450arom） and Cyp11a1（cholesterol side-chain cleavage enzyme P450,P450scc）,while significantly increased viable cell numbers and up-regulated expression of cyclin dependent kinase-4（CDK4） and cyclin D2.However,LH had no effect on the expression of BMP receptor genes.Thus,the present study indicates that the expression of members of the BMP signaling pathway in porcine GC is regulated during follicle development and the expression of BMP receptors are not regulated by LH in porcine GCs.
文摘目的:通过观察加味麻杏石甘汤对放射后肺泡Ⅱ型上皮细胞(AECⅡ)转化生长因子-β1(TGF-β1)m RNA、Smads表达的差异,探讨该方抗放射性肺损伤的分子机制。方法:清洁级SD大鼠18只,加味麻杏石甘汤灌胃给药3d,末次给药后1-2h采血,制备含药血清。用3.64Gy/min,总剂量8Gy的X线照射小鼠AECⅡ细胞,建立放射性损伤的AECⅡ细胞模型。RT-PCR法检测AECⅡTGF-β1、Smads m RNA水平;Western blot法检测AECⅡSmads蛋白的表达水平。结果:与正常大鼠血清组比较,含药血清组在干预48h后,AECⅡ细胞TGF-β1 m RNA及Smad2 m RNA表达降低(P<0.05),Smad6、Smad7 m RNA表达增高;10%、15%含药血清组p-Smad2、p-Smad3蛋白表达下调(P<0.05),Smad6和Smad7蛋白的表达增高(P<0.05)。结论:调控TGF-β1/Smad信号通路可能是加味麻杏石甘汤抑制放射性肺损伤的机制。
