Strawberry is a major fruit crop worldwide because its nutritional and health benefits to human health,but its productivity is limited by Botrytis cinerea.Sucrose nonfermentation 1-related protein kinase 1(SnRK1)has a...Strawberry is a major fruit crop worldwide because its nutritional and health benefits to human health,but its productivity is limited by Botrytis cinerea.Sucrose nonfermentation 1-related protein kinase 1(SnRK1)has a defense function against pathogens,but the function of SnRK1 in the defense response to B.cinerea in plants is still unclear.In this study,FaSnRK1a-OE and RNAi fruits were constructed and then inoculated with B.cinerea.The result reveals a positive role of Fa SnRK1a in the regulation of resistance to gray mold.FaSnRK1a affects SA content by regulating FaPAL1 and FaPAL2 expressions.The genes related to the SA signaling pathway(FaTGA1 and FaTGA2.1)were significantly increased/decreased in FaSnRK1a-OE or FaSnRK1a-RNAi fruit,respectively.FaSnRK1a interacted with the FaWRKY33.2 protein and negatively regulated FaWRKY33.2 expression,and FaWRKY33.2 acts as a repressor of disease resistance to B.cinerea.Finally,FaSnRK1a regulates the expression of six PR genes and the activities of antioxidant enzymes to boost defense response after B.cinerea inoculation.Our findings showed that FaSnRK1a increases the resistance of strawberry fruit to B.cinerea via SA signaling pathway and interaction with the FaWRKY33.2 transcription factor.展开更多
SNF1-related protein kinase 2(SnRK2)family members are essential components of the plant abscisic acid(ABA)signaling pathway initiated by osmotic stress and triggering a drought stress response.This study characterize...SNF1-related protein kinase 2(SnRK2)family members are essential components of the plant abscisic acid(ABA)signaling pathway initiated by osmotic stress and triggering a drought stress response.This study characterized the molecular properties of TaSnRK2.4 and its function in mediating adaptation to drought in Triticum aestivum.Transcripts of TaSnRK2.4 were upregulated upon drought and ABA signaling and associated with drought-and ABA-responsive cis-elements ABRE and DRE,and MYB and MYC binding sites in the promoter as indicated by reporter GUS protein staining and activity driven by truncations of the promoter.Yeast two-hybrid,BiFC,and Co-IP assays indicated that TaSnRK2.4 protein interacts with TaPP2C01 and an ABF transcription factor(TF)TaABF2.The results suggested that TaSnRK2.4 forms a functional TaPP2C01-TaSnRK2.4-TaABF2 module with its upstream and downstream partners.Transgene analysis revealed that TaSnRK2.4 and TaABF2 positively regulate drought tolerance whereas TaPP2C01 acts negatively by modulating stomatal movement,osmotic adjustment,reactive oxygen species(ROS)homeostasis,and root morphology.Expression analysis,yeast one-hybrid,and transcriptional activation assays indicated that several osmotic stress-responsive genes,including TaSLAC1-4,TaP5CS3,TaSOD5,TaCAT1,and TaPIN4,are regulated by TaABF2.Transgene analysis verified their functions in positively regulating stomatal movement(TaSLAC1-4),proline accumulation(TaP5CS3),SOD activity(TaSOD5),CAT activity(TaCAT1),and root morphology(TaPIN4).There were high correlations between plant biomass and yield with module transcripts in a wheat variety panel cultivated under drought conditions in the field.Our findings provide insights into understanding plant drought response underlying the SnRK2 signaling pathway in common wheat.展开更多
蔗糖非酵解型蛋白激酶(sucrose non-fermenting1-related protein kinase,SnRK)是广泛存在于植物中的一类Ser/Thr类蛋白激酶,参与植物体内多种信号途径的转导,在植物的抗逆境生理过程中扮演了重要角色。本文介绍了植物SnRK家族,重点阐述...蔗糖非酵解型蛋白激酶(sucrose non-fermenting1-related protein kinase,SnRK)是广泛存在于植物中的一类Ser/Thr类蛋白激酶,参与植物体内多种信号途径的转导,在植物的抗逆境生理过程中扮演了重要角色。本文介绍了植物SnRK家族,重点阐述了SnRK2的结构、相互作用蛋白及该激酶活性的调节。SnRK2可以被NaCl及ABA等渗透胁迫激活,调节一系列相关基因的表达,从而提高植物对逆境的抵抗能力。展开更多
SnRK2(sucrose non-fermenting 1一related protein kinase2)是脱落酸(ABA)信号转导途径中的正调控因子,广泛参与了植物的生长发育、病虫害防御、ABA和非生物胁迫等多种信号的应答反应。从番茄中分离了3个与SnRK2.2、SnRK2.3和SnRK2.6...SnRK2(sucrose non-fermenting 1一related protein kinase2)是脱落酸(ABA)信号转导途径中的正调控因子,广泛参与了植物的生长发育、病虫害防御、ABA和非生物胁迫等多种信号的应答反应。从番茄中分离了3个与SnRK2.2、SnRK2.3和SnRK2.6同源的基因,分别命名为SlSnRK2.2、SlSnRK2.3和SlSnRK2.6,同时构建了SlSlSnRK2.2/2.3/2.6 RNAi载体,并通过根癌农杆菌介导的叶盘法转化番茄,得到转基因番茄植株。初步表型分析结果发现转基因植株呈叶片早衰的现象。通过对乙烯信号相关基因的检测可知,转基因植株中除了ERF2表达量低于野生型,其余3个基因(ACO4、ERF1B、ERF1)的表达量均高于野生型,表明SlSnRK2.2、SlSnRK2.3和SlSnRK2.6与叶片衰老有关,可能参与ABA和乙烯信号互作,此结果对揭示植物衰老机理具有重要意义。展开更多
Sucrose non-fermenting-1 related protein kinase 2 （SnRK2） is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned a...Sucrose non-fermenting-1 related protein kinase 2 （SnRK2） is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. Atransient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde （MDA） content relative to the phenotypes observed in wild-type （WT） control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CSI. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.31672099,31801812)the National Modern Agroindustry Technology Research System Fund(Grant No.CARS-30-2-02)。
文摘Strawberry is a major fruit crop worldwide because its nutritional and health benefits to human health,but its productivity is limited by Botrytis cinerea.Sucrose nonfermentation 1-related protein kinase 1(SnRK1)has a defense function against pathogens,but the function of SnRK1 in the defense response to B.cinerea in plants is still unclear.In this study,FaSnRK1a-OE and RNAi fruits were constructed and then inoculated with B.cinerea.The result reveals a positive role of Fa SnRK1a in the regulation of resistance to gray mold.FaSnRK1a affects SA content by regulating FaPAL1 and FaPAL2 expressions.The genes related to the SA signaling pathway(FaTGA1 and FaTGA2.1)were significantly increased/decreased in FaSnRK1a-OE or FaSnRK1a-RNAi fruit,respectively.FaSnRK1a interacted with the FaWRKY33.2 protein and negatively regulated FaWRKY33.2 expression,and FaWRKY33.2 acts as a repressor of disease resistance to B.cinerea.Finally,FaSnRK1a regulates the expression of six PR genes and the activities of antioxidant enzymes to boost defense response after B.cinerea inoculation.Our findings showed that FaSnRK1a increases the resistance of strawberry fruit to B.cinerea via SA signaling pathway and interaction with the FaWRKY33.2 transcription factor.
基金supported by National Key Research and Development Program of China(2022YFD1200202)State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2022ZZ-7)Graduate Student Innovation Ability Training Funding Project of Hebei Province(CXZZBS2023073)。
文摘SNF1-related protein kinase 2(SnRK2)family members are essential components of the plant abscisic acid(ABA)signaling pathway initiated by osmotic stress and triggering a drought stress response.This study characterized the molecular properties of TaSnRK2.4 and its function in mediating adaptation to drought in Triticum aestivum.Transcripts of TaSnRK2.4 were upregulated upon drought and ABA signaling and associated with drought-and ABA-responsive cis-elements ABRE and DRE,and MYB and MYC binding sites in the promoter as indicated by reporter GUS protein staining and activity driven by truncations of the promoter.Yeast two-hybrid,BiFC,and Co-IP assays indicated that TaSnRK2.4 protein interacts with TaPP2C01 and an ABF transcription factor(TF)TaABF2.The results suggested that TaSnRK2.4 forms a functional TaPP2C01-TaSnRK2.4-TaABF2 module with its upstream and downstream partners.Transgene analysis revealed that TaSnRK2.4 and TaABF2 positively regulate drought tolerance whereas TaPP2C01 acts negatively by modulating stomatal movement,osmotic adjustment,reactive oxygen species(ROS)homeostasis,and root morphology.Expression analysis,yeast one-hybrid,and transcriptional activation assays indicated that several osmotic stress-responsive genes,including TaSLAC1-4,TaP5CS3,TaSOD5,TaCAT1,and TaPIN4,are regulated by TaABF2.Transgene analysis verified their functions in positively regulating stomatal movement(TaSLAC1-4),proline accumulation(TaP5CS3),SOD activity(TaSOD5),CAT activity(TaCAT1),and root morphology(TaPIN4).There were high correlations between plant biomass and yield with module transcripts in a wheat variety panel cultivated under drought conditions in the field.Our findings provide insights into understanding plant drought response underlying the SnRK2 signaling pathway in common wheat.
文摘蔗糖非酵解型蛋白激酶(sucrose non-fermenting1-related protein kinase,SnRK)是广泛存在于植物中的一类Ser/Thr类蛋白激酶,参与植物体内多种信号途径的转导,在植物的抗逆境生理过程中扮演了重要角色。本文介绍了植物SnRK家族,重点阐述了SnRK2的结构、相互作用蛋白及该激酶活性的调节。SnRK2可以被NaCl及ABA等渗透胁迫激活,调节一系列相关基因的表达,从而提高植物对逆境的抵抗能力。
文摘干旱、高盐、极端温度等逆境因子是限制作物产量和品质提高的重要因素。挖掘和利用逆境应答基因资源是改良其抗逆性的前提和基础,对于研究植物抗逆机制具有重要意义。蔗糖非发酵相关蛋白激酶家族2(Sucrose non-fermenting-1-related protein kinase 2,SnRK2)是广泛存在于植物中的一类Ser/Thr蛋白激酶,参与植物体内多种信号途径的转导,在植物的抗逆境生理过程中扮演了重要角色。为了促进小麦SnRK2基因家族的研究,该文对SnRK2基因的结构、抗逆功能、互作蛋白,以及小麦SnRK2基因家族的研究现状进行了阐述。
基金supported by the National High Technology R&D Program of China (2012AA10A306)the National Natural Science Foundation of China (31330056)the Xinjiang High-Tech Research Projects, China (201011109)
文摘Sucrose non-fermenting-1 related protein kinase 2 （SnRK2） is a unique family of protein kinases associated with abiotic stress signal transduction in plants. In this study, a maize SnRK2 gene ZmSnRK2.11 was cloned and characterized. The results showed that ZmSnRK2.11 is up-regulated by high-salinity and dehydration treatment, and it is expressed mainly in maize mature leaf. Atransient expression assay using onion epidermal cells revealed that ZmSnRK2.11-GFP fusion proteins are localized to both the nucleus and cytoplasm. Overexpressing-ZmSnRK2.11 in Arabidopsis resulted in salt and drought sensitivity phenotypes that exhibited an increased rate of water loss, reduced relative water content, delayed stoma closure, accumulated less free proline content and increased malondialdehyde （MDA） content relative to the phenotypes observed in wild-type （WT） control. Furthermore, overexpression of ZmSnRK2.11 up-regulated the expression of the genes ABI1 and ABI2 and decreased the expression of DREB2A and P5CSI. Taken together, our results suggest that ZmSnRK2.11 is a possible negative regulator involved in the salt and drought stress signal transduction pathways in plants.
