Impact on Soil Organic C and Total Soil N from Cool- and Warm-Season Legumes Used in a Green Manure-Forage Cropping System

Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha-1) and contributed the most total N (167 kg∙ha-1) and total C (3043 kg∙ha-1) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha-1 with a total N yield of 319 kg N ha-1 and total C production of 3835 kg C ha-1. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition.

酸化对水稻-土壤系统氮分配和N_(2)O排放的影响

为研究土壤酸化对水稻-土壤系统氮转化、分配和氮损失的影响,以水稻-土壤系统为研究对象,设置中性(pH 7,CK)、弱酸(pH 6,T1)、中强酸(pH 5,T2)和强酸(pH 4,T3)4个土壤递增酸度处理,比较了不同酸度下水稻产量、氮素积累量、氮代谢酶活性、氮素利用效率、氮平衡和N_(2)O排放等指标的差异。结果表明,随着土壤酸度增加,水稻植株氮素积累、利用效率和产量呈现先增加后降低的趋势。相关性分析表明,拔节期氮素积累量与叶片中硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)和谷氨酸脱氢酶(GDH)以及茎秆中GS和GOGAT活性呈显著正相关;开花期氮素积累量与穗中的NR、GS、GOGAT和GDH活性呈显著正相关。T1、T2和T3处理N_(2)O累积排放量与CK处理相比分别降低20.3%、58.0%和76.7%;单位产量下的N_(2)O排放量呈现递减的趋势。氮平衡分析表明,相比于CK处理,T2和T3处理氮素表观损失分别降低15.8%和21.1%,水稻氮吸收量分别降低1.5%和15.3%,土壤无机氮残留量分别增加41.2%和88.2%,氮素盈余率分别提高2.2个和7.1个百分点。土壤酸化至中强酸和强酸时,会分别通过抑制水稻拔节期茎叶和开花期穗部的氮代谢过程降低氮素积累量、利用效率和产量。土壤酸化会降低稻田N_(2)O累积排放量,同时也会降低单位产量N_(2)O排放量。此外,中强酸和强酸度土壤还会通过降低水稻氮吸收量和增加播前土壤无机氮量,提高土壤无机氮残留量和氮素盈余率,增加环境风险。

Long-Term Application of Organic Manure and Mineral Fertilizer on N_2O and CO_2 Emissions in a Red Soil from Cultivated Maize-Wheat Rotation in China

A long-term field experiment was established to determine the influence of mineral fertilizer and organic manure on soil fertility. A tract of red soil （Ferralic Cambisol） in Qiyang Red Soil Experimental Station （Qiyang County, Hunan Province, China） was fertilized beginning in 1990 and N20 and CO2 were examined during the maize and wheat growth season of 2007-2008. The study involved five treatments： organic manure （NPKM）, fertilizer NPK （NPK）, fertilizer NP （NP）, fertilizer NK （NK）, and control （CK）. Manured soils had higher crop biomass, organic C, and pH than soils receiving the various mineralized fertilizers indicating that long-term application of manures could efficiently prevent red soil acidification and increase crop productivity. The application of manures and fertilizers at a rate of 300 kg N ha-1 yr-1 obviously increased NzO and CO2 emissions from 0.58 kg N20-N ha-~ yr-~ and 10565 kg C ha-~ yr-~ in the CK treatment soil to 3.0l kg N20-N ha-~ yr-~ and 28 663 kg C ha-~ yr-I in the NPKM treatment. There were also obvious different effects on N20 and CO2 emissions between applying fertilizer and manure. More N20 and CO2 released during the 184-d maize growing season than the 125- d wheat growth season in the manure fertilized soils but not in mineral fertilizer treatments. N20 emission was significantly affected by soil moisture only during the wheat growing season, and CO2 emission was affected by soil temperature only in CK and NP treatment during the wheat and maize growing season. In sum, this study indicates the application of organic manure may be a preferred strategy for maintaining red emissions than treatments only with mineral fertilizer. soil productivity, but may result in greater N20 and CO2

Effects of Soluble Organic N on Evaluating Soil N-Supplying Capacity

It is important to study the soluble organic N （SON） extracted during water-logged incubation for evaluating soil Nsupplying capacity. Soil initial SON and mineral N （Nmin）, cumulative soluble organic N and NH4＋-N in leachates during water-logged incubation, mineralization potentials of both easily decomposable N （ND） and resistant N （NR）, and their relationships with N uptake by crop in pot experiment were investigated by using 10 kinds of farmland soils with widely different physical and chemical properties on the Loess Plateau, China, and the effects of SON on evaluating soil Nsupplying capacity were studied. The results showed that the average content of initial SON （23.9 mg kg^-1） of 10 soils was 28.8% of initial total soluble N and 2.4% of soil total N. The percentage of cumulative SON in leaching total soluble N （118.1 mg kg^-1 was 46.4%, higher than the percentage of initial SON （28.8%）, and almost close to the percentage of cumulative NH4^＋-N in the leachates. ND had close correlation with total N, and the correlation coefficients were 0.92 （P 〈 0.01, excluding SON in estimating ND） and 0.88 （P 〈 0.01, including SON in estimating ND）, respectively. N mineralization potential and mineralization rate constant were different with the soil types. ND of Los-Orthic Entisols and Ust-Sandiic Entisols were lower than that of Eum-Orthrosols. Mineralization rate constant for the fast decomposable N-fraction （kD） decreased and the mineralization rate constant of resistant materials （kR） increased when SON was taken into account. Cumulative NH4^＋-N was a better evaluation index of soil N-supplying capacity, and it is not only suitable for the first season crops but also for two successive season crops. Cumulative SON alone was not a satisfactory index for the potential of mineralizable N. But it would be more accurate for ND in revealing the potential mineralizable N when SON was taken into account. Cumulative TSN, to some extent, could also be taken as an index for the potential mineralizable N. Cumulative NH4＋-N, total soluble N, and ND were good indexes for estimating soil potential mineralizable N, especially for soils of two successive season crops. And cumulative total soluble N and ND in evaluating the permanence of soil N-supply is of greater significance when SON was included.

Effects of six years of simulated N deposition on gross soil N transformation rates in an old-growth temperate forest

Elevated atmospheric nitrogen(N) deposition has been detected in many regions of China, but its effects on soil N transformation in temperate forest ecosystems are not well known. We therefore simulated N deposition with four levels of N addition rate(N0, N30, N60, and N120) for6 years in an old-growth temperate forest in Xiaoxing’an Mountains in Northeastern China. We measured gross N transformation rates in the laboratory usingN tracing technology to explore the effects of N deposition on soil gross N transformations taking advantage of N deposition soils. No significant differences in gross soil N transformation rates were observed after 6 years of N deposition with various levels of N addition rate. For all N deposition soils, the gross NH~+ immobilization rates were consistently lower than the gross N mineralization rates,leading to net N mineralization. Nitrate(NO~-) was primarily produced via oxidation of NH~+(i.e., autotrophic nitrification), whereas oxidation of organic N(i.e., heterotrophic nitrification) was negligible. Differences between the quantity of ammonia-oxidizing bacteria and ammonia-oxidizing archaea were not significant for any treatment, which likely explains the lack of a significant effect on gross nitrification rates. Gross nitrification rates were much higher than the total NO~- consumption rates,resulting in a build-up of NO~-, which highlights the high risk of N losses via NO~- leaching or gaseous N emissions from soils. This response is opposite that of typical N-limited temperate forests suffering from N deposition,suggesting that the investigated old-growth temperate forest ecosystem is likely to approach N saturation.

Soil mineral nitrogen and yield-scaled soil N2O emissions lowered by reducing nitrogen application and intercropping with soybean for sweet maize production in southern China

The increasing demand for fresh sweet maize （Zea mays L. saccharata） in southern China has prioritized the need to find solutions to the environmental pollution caused by its continuous production and high inputs of chemical nitrogen fertilizers. A promising method for improving crop production and environmental conditions is to intercrop sweet maize with legumes. Here, a three-year field experiment was conducted to assess the influence of four different cropping systems （sole sweet maize （SS）, sole soybean （SB）, two rows sweet maize-three rows soybean （S2B3） intercropping, and two rows sweet maize-four rows soybean （S2B4） intercropping）, together with two rates of N fertilizer application （300 and 360 kg N ha-1） on grain yield, residual soil mineral N, and soil N2O emissions in southern China. Results showed that in most case, inter- cropping achieved yield advantages （total land equivalent ratio （TLER=0.87-1.25） was above one）. Moreover, intercropping resulted in 39.8% less soil mineral N than SS at the time of crop harvest, averaged over six seasons （spring and autumn in each of the three years of the field experiment）. Generally, intercropping and reduced-N application （300 kg N ha-1） produced lower cumulative soil N20 and yield-scaled soil N20 emissions than SS and conventionaI-N application （360 kg N ha-l）, respectively. $2B4 intercropping with reduced-N rate （300 kg N ha-~） showed the lowest cumulative soil N20 （mean value=0.61 kg ha-1） and yield-scaled soil N20 （mean value=0.04 kg t-1） emissions. Overall, intercropping with reduced-N rate maintained sweet maize production, while also reducing environmental impacts. The system of S2B4 intercropping with reduced-N rate may be the most sustainable and environmentally friendly cropping system.

Distribution of Crystal Organic Fertilizer-N in Soil-plant System

The distribution of crystal organic fertilizer, urea and compound fertilizer-N in soil and plant system was researched with 15N-trace under tobacco pot experiment. The results showed that the leaf yield of tobacco used crystal organic fertilizer was 23.1% and 14.6% higher than that of urea and compound fertilizer treatments respectively. Compound fertilizer also resulted in higher yield of 8.5 % comparing with the urea treatment. Nitrogen content of the plant from the crystal organic fertilizer treatment was 138. 6% and 145.7% as high as that of the compound fertilizer and urea treatments respectively. The absorbed N from the organic fertilizer was 25.1% and 27.9% more than that from the compound fertilizer and urea respectively. However, the absorbed N from the soil with the organic fertilizer was 47.4% and 58.3% more than that with compound fertilizer and urea respectively. The N use efficiency of the organic fertilizer was 9.4% and 10.1% higher than that of the compound fertilizer and urea. It indicated that the crystal organic fertilizer not only had high N use efficiency, but also stimulated tobacco taking up more N from soil.

Treatment of turtle-breeding wastewater and domestic fecal sewage with soil cultivating system

Turtle-breeding wastewater and domestic fecal sewage were treated by means of soil cultivating system. Results indicated that more than 50% CODCr and BOD5, of wastewaters were removed, removal rates of NH4+ -N could reach about 70%-80%, but PO43- could not be removed. The thesis analyzed functional mechanisms for pollutants and put forward main elements affecting treatment efficiencies, thus provided conditions for further research.

Soil pH Dynamics and Nitrogen Transformations Under Long-Term Chemical Fertilization in Four Typical Chinese Croplands

Long-term fertilization experiment provides the platform for understanding the proton budgets in nitrogen transformations of agricultural ecosystems. We analyzed the historical （1990-2005） observations on four agricultural long-term experiments in China （Changping, Chongqing, Gongzhuling and Qiyang） under four different fertilizations, i.e., no-fertilizer （control）, sole chemical nitrogen fertilizer （FN）, sole chemical phosphorous and potassium fertilizers （FPK） and chemical nitrogen, phosphorous and potassium fertilizers （FNPK）. The significant decline in topsoil pH was caused not only by chemical N fertilization （0.29 and 0.89？pH at Gongzhuling and Qiyang, respectively） but also by chemical PK fertilization （0.59？pH at Gongzhuling）. The enhancement of available nutrients in the topsoil due to long-term direct nutrients supply with chemical fertilizers was in the descending order of available P （168-599%）〉available K （16-189%）〉available N （9-33%）. The relative rate of soil pH decline was lower under long-term judicious chemical fertilization （-0.036-0.034 ？pH yr-1） than that under long-term sole N or PK fertilization （0.016-0.086 ？pH yr-1）. Long-term judicious chemical fertilization with N, P and K elements decreases the nutritional limitation to normal crop growth, under which more N output was distributed in biomass removal rather than the loss via nitrate leaching. We concluded that the N distribution percentage of nitrate leaching to biomass removal might be a suitable indicator to the sensitivity of agricultural ecosystems to acid inputs.

Soil respiration response to precipitation reduction in a grassland and a Mongolian pine plantation in semi-arid northeast China

Climate change is predicted to alter global precipitation regimes.However,the response of soil carbon and nitrogen cycles and soil microorganisms to precipitation reduction is poorly understood but is dependent on ecosystem type.To evaluate the impacts of reduced precipitation on soil respiration,soil inorganic nitrogen(i.e.,NH4^+–N and NO3^-–N),nitrogen mineralization,and soil microbial community composition,a precipitation manipulation experiment was initiated in a Mongolian pine plantation and a naturally restored grassland in semi-arid northeast China.Precipitation reduction led to decreases of soil respiration rates by 14 and 8%in 2014 and 2015 in the Mongolian pine plantation but no changes in the grassland.Soil inorganic nitrogen,ammonification and nitrification rate,and soil phospholipids fatty acids were not significantly changed by reduced precipitation but significantly differed between the two ecosystems and among growing seasons.Our results suggest that the impacts of precipitation reduction on soil respiration were different between the Mongolian pine plantation and the grassland,and that ecosystem type and growing season had more pronounced impacts on soil carbon and nitrogen cycles.

Change of soil productivity in three different soils after long-term field fertilization treatments

Soil productivity(SP) without external fertilization influence is an important indicator for the capacity of a soil to support crop yield.However,there have been difficulties in estimating values of SPs for soils after various long-term field treatments because the treatment without external fertilization is used but is depleted in soil nutrients,leading to erroneous estimation.The objectives of this study were to estimate the change of SP across different cropping seasons using pot experiments,and to evaluate the steady SP value(which is defined by the basal contribution of soil itself to crop yield) after various longterm fertilization treatments in soils at different geographical locations.The pot experiments were conducted in Jinxian of Jiangxi Province with paddy soil,Zhengzhou of Henan Province with fluvo-aquic soil,and Gongzhuling of Jilin Province with black soils,China.Soils were collected after long-term field fertilization treatments of no fertilizer(control;CK-F),chemical fertilizer(NPK-F),and combined chemical fertilizer with manure(NPKM-F).The soils received either no fertilizer(F0) or chemical fertilizer(F1) for 3-6 cropping seasons in pots,which include CK-P(control;no fertilizer from long-term field experiments for pot experiments),NPK-P(chemical fertilizer from long-term field experiments for pot experiments),and NPKM-P(combined chemical and organic fertilizers from long-term field experiments for pot experiments).The yield data were used to calculate SP values.The initial SP values were high,but decreased rapidly until a relatively steady SP was achieved at or after about three cropping seasons for paddy and fluvo-aquic soils.The steady SP values in the third cropping season from CK-P,NPK-P,and NPKM-P treatments were 37.7,44.1,and 50.0% in the paddy soil,34.2,38.1,and 50.0% in the fluvo-aquic soil,with the highest value observed in the NPKM-P treatment for all soils.However,further research is required in the black soils to incorporate more than three cropping seasons.The partial least squares path mode(PLS-PM) showed that total N(nitrogen) and C/N ratio(the ratio of soil organic carbon and total N) had positive effects on the steady SP for all three soils.These findings confirm the significance of the incorporation of manure for attaining high soil productivity.Regulation of the soil C/N ratio was the other main factor for steady SP through fertilization management.

Nitrogen mobility,ammonia volatilization,and estimated leaching loss from long-term manure incorporation in red soil

Nitrogen（N） loss from fertilization in agricultural fields has an unavoidable negative impact on the environment and a better understanding of the major pathways can assist in developing the best management practices. The aim of this study was to evaluate the fate of N fertilizers applied to acidic red soil（Ferralic Cambisol） after 19 years of mineral（synthetic） and manure fertilizer treatments under a cropping system with wheat-maize rotations. Five field treatments were examined： control（CK）, chemical nitrogen and potash fertilizer（NK）, chemical nitrogen and phosphorus fertilizer（NP）, chemical nitrogen, phosphorus and potash fertilizer（NPK） and the NPK with manure（NPKM, 70% N from manure）. Based on the soil total N storage change in 0–100 cm depth, ammonia（NH_3） volatilization, nitrous oxide（N_2O） emission, N plant uptake, and the potential N leaching loss were estimated using a mass balance approach. In contrast to the NPKM, all mineral fertilizer treatments（NK, NP and NPK） showed increased nitrate（NO_3~–） concentration with increasing soil depth, indicating higher leaching potential. However, total NH_3 volatilization loss was much higher in the NPKM（19.7%） than other mineral fertilizer treatments（≤4.2%）. The N_2O emissions were generally low（0.2–0.9%, the highest from the NPKM）. Total gaseous loss accounted for 1.7, 3.3, 5.1, and 21.9% for NK, NP, NPK, and NPKM treatments, respectively. Estimated N leaching loss from the NPKM was only about 5% of the losses from mineral fertilizer treatments. All data demonstrated that manure incorporation improved soil productivity, increased yield, and reduced potential leaching, but with significantly higher NH_3 volatilization, which could be reduced by improving the application method. This study confirms that manure incorporationis an essential strategy in N fertilization management in upland red soil cropping system.

Role of nitrification inhibitor DMPP(3,4-dimethylpyrazole phosphate) in NO^-_3-N accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil

The influence of nitrification inhibitor(NI) 3,4 dimethylpyrazole phosphate(DMPP) on nitrate accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003 Results showed that NI DMPP took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease NO - 3 N concentration in greengrocery and in vegetable soil at surface layer. In addition, NI DMPP could reduce the NO - 3 N concentration during the prophase stage of storage.

Small Scale Spatio-Temporal Variabilities in Soil Nitrogen, Leaf Nitrogen, and Canopy Normalized Difference Vegetation Index of Cotton

Strip plots have been increasingly used in agricultural field experiments to better reflect the true situation of crop production on farmers’ fields, but failure to account for spatially and temporally related errors when present in the data analysis of strip plot field experiments may cause inefficient assessment of treatment effect significance. The objective of this study was to investigate patterns and degrees of the spatial and temporal variabilities in soil inorganic N level, leaf N concentration, and Normalized Difference Vegetation Index (NDVI) of cotton under no-tillage and the influences of N treatments on these variabilities. A strip plot experiment was conducted on a private farm near Brazil, Gibson County, Tennessee from 2009 through 2011. Five N treatments of 0, 45, 90, 134, and 179 kg N ha-1 were implemented as side dress N in strip plots under a randomized complete block design with three replicates after 45 kg N ha-1 was applied in the form of chicken litter before cotton planting. Spatial variability was present in soil inorganic N before cotton planting and after harvest, and in leaf N and canopy NDVI at the early square and early, mid-, and late bloom stages although the patterns and degrees of the spatial variabilities sometimes varied with growth stages and years. Application of the in-season side-dress N treatments often reduced the spatial variations of leaf N and NDVI, but increased those of post-harvest soil inorganic N. Out results suggest that the spatial and temporal variabilities of soil inorganic N, leaf N, and NDVI are high, and should be taken into account if possible in the data analyses of N treatment effects on related soil properties and plant characteristics of cotton in strip plot field experiment research.

C/N配施对旱作谷子根系形态分布及土壤养分的影响

为明确不同C/N配比对谷子的根系形态分布及土壤养分的影响,研究以晋谷21号为研究对象,设置5个处理分别为C115 g/m^(2)生物炭、C220 g/m^(2)生物炭、C325 g/m^(2)生物炭,N13 g/m^(2)氮肥、N29 g/m^(2)氮肥交互的处理。结果表明:拔节期C2N2谷子根系长度、根系总表面积、根系体积增幅分别为134.06%、293.40%、500.00%;成熟期C2N2土壤铵态氮、硝态氮、速效磷、速效钾、有机碳增幅分别为149.64%、139.00%、10.53%、20.83%和136.00%,综上所述,C2N2配比能显著促进晋谷21根系的生长发育,调控土壤养分,存在最佳的配比。

Normal Alkane Distributions in Soil Samples along a Lhasa-Bharatpur Transect

We present n-alkane distributions in the soil samples along a transect from Lhasa on the Tibetan Plateau to Bharatpur in Nepal, which covers a large geographical area and a wide range of climatic conditions. These data allow us to assess the significance of n-alkane distributions in different vegetation types and their relationships to temperature and precipitation. In the tropical rainforest and broadleaved forest zones, n-alkanes exhibit a bimodal distribution pattern with dominant homologues around n-C_（31） and n-C_（23）. The bimodal distribution of tropical rainforest n-alkanes may be a general pattern because of the presence of many lianas, epiphytic plants, algae and the strong microbial activity and degradation involved in the postdepositional process. In the warm-temperate mixed forest and needle-leaved forest zones, the long-chain alkanes have a pattern of n-C_（31） n-C_（33） n-C_（27）. In the alpine shrub and grassland zone, although the most abundant homologue is n-C_（31） , relatively high n-C_（23） concentrations have also been observed in some samples. The statistical results show a good correlation between n-alkane proxies and climatic factors. The average chain length（ACL） values are positively correlated with precipitation and temperature across the transect. The carbon preference index（CPI） values show a negative correlation with temperature and precipitation along a Lhasa–Bharatpur transect. Although there is a positive relationship between ACL and temperature, it is difficult to separate the two climatic variables（temperature and precipitation） because they are well coupled in the monsoon region.

Short-term effects of nitrogen deposition on soil respiration components in two alpine coniferous forests, southeastern Tibetan Plateau

Nitrogen (N) deposition to alpine forest ecosystems is increasing gradually, yet previous studies have seldom reported the effects of N inputs on soil CO2 flux in these ecosystems. Evaluating the effects of soil respiration on N addition is of great significance for understanding soil carbon (C) budgets along N gradients in forest ecosystems. In this study, four levels of N (0, 50, 100, 150 kg N ha^-1 a^-1) were added to soil in a Picea baifouriana and an Abies georgei natural forest on the Tibetan Plateau to investigate the effect of the N inputs on soil respiration. N addition stimulated total soil respiration (Rt) and its components including heterotrophic respiration (Rh) and autotrophic respiration (Ra);however, the promoted effects declined with an increase in N application in two coniferous forests. Soil respiration rate was a little greater in the spruce forest (1.05 μmol CO2 m^-2 s^-1) than that in the fir forest (0.97 μmol CO2 m^-2 s^-1). A repeated measures ANOVA indicated that N fertilization had significant effects on Rt and its components in the spruce forest and Rt in the fir forest, but had no obvious effect on Rh or Ra in the fir forest. Rt and its components had significant exponential relationships with soil temperature in both forests. N addition also increased temperature sensitivity (Q10) of Rt and its components in the two coniferous forests, but the promotion declined as N in put increased. Important, soil moisture had great effects on Rt and its components in the spruce forest (P<0.05), but no obvious impacts were observed in the fir forest (P>0.05). Following N fertilization, Ra was significantly and positively related to fine root biomass, while Rh was related to soil enzymatic activities in both forests. The mechanisms underlying the effect of simulated N deposition on soil respiration and its components in this study may help in forecasting C cycling in alpine forests under future levels of reactive N deposition.

Short-term residual effects of various amendments on organic C and N, and available nutrients in soil under organic crop production

Two 3-year (2008-2010, wheat-pea-barley) field experiments were conducted on certified organic farms near Spalding (Dark Brown Chernozem-Typic Haploboroll) and Star City (Gray Luvisol-Typic Haplocryalf) in northeastern Sas-katchewan, Canada, to determine the residual effects of compost, alfalfa pellets, wood ash, rock phosphate, Penicillium bilaiae, gypsum and MykePro on organic C and N (total organic C [TOC], total organic N [TON], light fraction organic C [LFOC], light fraction organic N [LFON]) and mineralizable N (Nmin) in the 0 - 15 cm soil layer, and ammonium-N, nitrate-N, extractable P, exchangeable K and sulphate-S in the 0 - 15, 15 - 30 and 30 - 60 cm soil layers in autumn 2010. Compared to the unamended control, mass of TOC, TON, LFOC and LFON increased with compost and alfalfa pellets in both soils. However, the increases were much more pronounced for LFOC (by 125% - 133%) or LFON (by 102% - 103%) than TOC (by 19% - 29%) or TON (by 25% - 40%). The Nmin also increased in these two treatments compared to the control, but the increases were much smaller for compost than alfalfa pellets. In general, residual nitrate-N increased with increasing rate of compost and alfalfa pellets in the 0 - 15 and 15 - 30 cm layers in both soils. Extractable P increased with compost and exchangeable K with alfalfa pellets, but only in the 0 - 15 cm soil layer. Sulphate-S increased with compost, but mainly in the 30 - 60 cm soil layer. Soil pH usually increased with compost and more so with wood ash, but no effect of any amendment on ammonium-N. Overall, the quantity of organic C and N, and available nutrients in soil increased with compost and/or alfalfa pellets, but the magnitude varied with amendment and/or soil type. In conclusion, our findings suggest that soil quality and fertility can be improved with these organic amendments, suggesting sustainability of production from organic crops.

秸秆与生物炭对棉田碱性土壤NH_(3)挥发与N_(2)O排放的影响

为探究秸秆和秸秆生物炭连续添加5 a后对土壤氨(NH_(3))挥发和氧化亚氮(N_(2)O)排放的影响,并确定合理的秸秆还田措施,以降低碱性棉田氮损失。本研究基于等碳量输入,设置秸秆翻埋、秸秆催腐+覆盖还田、秸秆生物炭翻埋和不还田对照共4个处理,氮磷钾肥统一施用。结果表明:秸秆生物炭翻埋下土壤NH_(3)挥发和N_(2)O排放分别较不还田对照显著降低27.3%和56.7%,主要归因于生物炭显著抑制土壤羟胺还原酶与硝酸还原酶活性,增加棉花氮吸收量,也与生物炭自身的强吸附能力有关。而秸秆翻埋、秸秆催腐+覆盖还田分别较对照增加NH3挥发37.2%和21.2%,但减少N2O排放17.1%和38.3%,这两种秸秆还田方式均显著促进土壤有机氮矿化和羟胺还原酶活性,抑制硝酸还原酶活性。冗余分析(RDA)结果表明羟胺还原酶和棉花氮吸收是土壤NH3挥发和N_(2)O排放的主要影响因子,解释率分别为64.8%和20.1%。研究表明,秸秆生物炭翻埋对NH_(3)和N_(2)O减排的综合效果优于秸秆,是碱性棉田土壤值得推荐的氮减排措施。

超声萃取-高效液相色谱-串联质谱法测定土壤中三种硝基酚类化合物

硝基酚类化合物作为一类有机化工原料,具有高毒性和致癌性,可通过空气、水传播进入土壤造成污染,危害人类健康。以往采用气相色谱法和气相色谱-质谱法直接测定硝基酚类化合物,存在色谱灵敏度低、峰形差等问题。为提高灵敏度,通常需要对硝基酚类化合物进行衍生化处理,但衍生化方法步骤繁琐耗时。为实现土壤中硝基酚类化合物的快速准确分析,本文建立了超声萃取-高效液相色谱-串联质谱法分析土壤中三种硝基酚类化合物的方法。用二氯甲烷-正己烷(2∶1,V/V)混合溶剂超声萃取土壤中硝基酚类化合物,在超声萃取液中加入强碱性水溶液(pH>12),将下层有机相除去。将水溶液调至酸性(pH<2)后,用二氯甲烷-乙酸乙酯(4∶1,V/V)混合溶剂萃取三种硝基酚类化合物。萃取液浓缩后用10%乙腈-水溶液定容至10.0mL,高效液相色谱-串联质谱仪测定,外标法定量。性能评价的结果表明方法精密度(RSD<10%,n=6)和准确度都可以满足测定要求,三种硝基酚类化合物的方法检出限为0.1~0.2μg/kg,加标回收率为61.7%~90.8%。本方法具有前处理简单、检出限低等优点,可应用于土壤中硝基酚类化合物的测定和评估。