目的探讨血清可溶性白细胞介素-2受体(sIL-2R)、降钙素原、微小RNA(miR)-223与新生儿肺炎病情程度及转归的关系。方法回顾性选取2018年1月至2023年6月池州市第二人民医院新生儿科收治的80例新生儿肺炎患儿作为肺炎组,另外选取本院同期...目的探讨血清可溶性白细胞介素-2受体(sIL-2R)、降钙素原、微小RNA(miR)-223与新生儿肺炎病情程度及转归的关系。方法回顾性选取2018年1月至2023年6月池州市第二人民医院新生儿科收治的80例新生儿肺炎患儿作为肺炎组,另外选取本院同期出生的健康新生儿80名作为对照组。对比两组的血清sIL-2R、降钙素原、miR-223水平。并按照患儿病情程度、治疗转归进行分组,其中,轻度组患儿44例,重度组患儿36例;经过治疗,预后良好组患儿63例,预后不良组患儿17例。对比不同病情程度、不同转归新生儿肺炎患儿血清sIL-2R、降钙素原、miR-223水平。采用多因素Logistic回归模型分析血清sIL-2R、降钙素原、miR-223与患儿治疗转归结局的关系。结果肺炎组患儿血清sIL-2R、降钙素原、miR-223水平分别为(32.40±8.94)μg/mL、(10.40±2.91)ng/mL、9.84±2.77,均显著高于对照组[(15.48±3.84)μg/mL、(5.84±1.50)ng/mL、6.10±1.84],差异均有统计学意义(P<0.05)。重度组患儿的血清sIL-2R、降钙素原、miR-223水平分别为(36.63±8.70)μg/mL、(12.44±2.70)ng/mL、11.65±2.32,均显著高于轻度组[(28.94±6.13)μg/mL、(8.73±2.65)ng/mL、8.36±2.45],差异均有统计学意义(P<0.05)。预后不良组患儿的血清sIL-2R、降钙素原、miR-223水平分别为(40.85±6.92)μg/mL、(15.70±2.56)ng/mL、15.10±2.13,均显著高于预后良好组[(30.12±8.77)μg/mL、(8.97±2.43)ng/mL、8.42±2.65],差异均有统计学意义(P<0.05)。不同分娩孕周(≥37周vs.<37周)、不同出生体重(≥2500 g vs.<2500 g)、不同出生1 min Apgar评分(≥8分vs.<8分)的新生儿肺炎患儿的转归结局比较,差异均有统计学意义(P<0.05);Logistic回归模型分析,结果显示:分娩孕周<37周、出生体重<2500 g、出生1 min Apgar评分<8分及血清sIL-2R升高、降钙素原升高、miR-223升高是新生儿肺炎患儿治疗转归结局不良的独立危险因素(P<0.05)。结论新生儿肺炎患儿血清sIL-2R、降钙素原、miR-223水平显著提高,并且与患儿病情程度、不良预后结局关系密切。展开更多
Background: Cytokines are mediators of diseases. Expression levels in the blood could be of clinical relevance. Objective: sIL-2Rα is used as a marker for different malignancies in human medicine. The aim of this stu...Background: Cytokines are mediators of diseases. Expression levels in the blood could be of clinical relevance. Objective: sIL-2Rα is used as a marker for different malignancies in human medicine. The aim of this study was to show if sIL-2Rα is detectable and if there is any correlation to different diseases in dogs. Methods: For this purposes sIL-2Rα concentrations in the blood were measured in healthy dogs, in dogs with different non-neoplastic diseases and benign tumors and in dogs with malignant tumors. Serum levels of sIL-2Rα were measured by using a human specific enzyme linked immunosorbent assay (ELISA). Results: Measurement of sIL-2Rα was successful in most of the samples. Dogs with diseases have significantly increased serum levels of sIL-2Rα compared to healthy controls. sIL-2Rα serum levels are higher in patients with non-neoplastic diseases and benign tumors than in those with malignant neoplasia. There is a strong correlation between sIL-2Rα and leukocyte count. Conclusion: Measurements of sIL-2Rα in serum may be helpful in detecting stages and grades of inflammation in the progression of disease. sIL-2Rα could actually not be used as an indicator for malignant diseases in dogs like in humans. The strong correlation between sIL-2Rα and the leukocyte count indicates the inflammatory response to the disease. This could be helpful in giving a prognosis in some cases, because the inflammatory reaction is of prognostic relevance in different diseases including malignant and non-malignant neoplasia. Although the results of our research studies were very promising, further studies should be performed with a canine ELISA.展开更多
文摘目的探讨血清可溶性白细胞介素-2受体(sIL-2R)、降钙素原、微小RNA(miR)-223与新生儿肺炎病情程度及转归的关系。方法回顾性选取2018年1月至2023年6月池州市第二人民医院新生儿科收治的80例新生儿肺炎患儿作为肺炎组,另外选取本院同期出生的健康新生儿80名作为对照组。对比两组的血清sIL-2R、降钙素原、miR-223水平。并按照患儿病情程度、治疗转归进行分组,其中,轻度组患儿44例,重度组患儿36例;经过治疗,预后良好组患儿63例,预后不良组患儿17例。对比不同病情程度、不同转归新生儿肺炎患儿血清sIL-2R、降钙素原、miR-223水平。采用多因素Logistic回归模型分析血清sIL-2R、降钙素原、miR-223与患儿治疗转归结局的关系。结果肺炎组患儿血清sIL-2R、降钙素原、miR-223水平分别为(32.40±8.94)μg/mL、(10.40±2.91)ng/mL、9.84±2.77,均显著高于对照组[(15.48±3.84)μg/mL、(5.84±1.50)ng/mL、6.10±1.84],差异均有统计学意义(P<0.05)。重度组患儿的血清sIL-2R、降钙素原、miR-223水平分别为(36.63±8.70)μg/mL、(12.44±2.70)ng/mL、11.65±2.32,均显著高于轻度组[(28.94±6.13)μg/mL、(8.73±2.65)ng/mL、8.36±2.45],差异均有统计学意义(P<0.05)。预后不良组患儿的血清sIL-2R、降钙素原、miR-223水平分别为(40.85±6.92)μg/mL、(15.70±2.56)ng/mL、15.10±2.13,均显著高于预后良好组[(30.12±8.77)μg/mL、(8.97±2.43)ng/mL、8.42±2.65],差异均有统计学意义(P<0.05)。不同分娩孕周(≥37周vs.<37周)、不同出生体重(≥2500 g vs.<2500 g)、不同出生1 min Apgar评分(≥8分vs.<8分)的新生儿肺炎患儿的转归结局比较,差异均有统计学意义(P<0.05);Logistic回归模型分析,结果显示:分娩孕周<37周、出生体重<2500 g、出生1 min Apgar评分<8分及血清sIL-2R升高、降钙素原升高、miR-223升高是新生儿肺炎患儿治疗转归结局不良的独立危险因素(P<0.05)。结论新生儿肺炎患儿血清sIL-2R、降钙素原、miR-223水平显著提高,并且与患儿病情程度、不良预后结局关系密切。
文摘Background: Cytokines are mediators of diseases. Expression levels in the blood could be of clinical relevance. Objective: sIL-2Rα is used as a marker for different malignancies in human medicine. The aim of this study was to show if sIL-2Rα is detectable and if there is any correlation to different diseases in dogs. Methods: For this purposes sIL-2Rα concentrations in the blood were measured in healthy dogs, in dogs with different non-neoplastic diseases and benign tumors and in dogs with malignant tumors. Serum levels of sIL-2Rα were measured by using a human specific enzyme linked immunosorbent assay (ELISA). Results: Measurement of sIL-2Rα was successful in most of the samples. Dogs with diseases have significantly increased serum levels of sIL-2Rα compared to healthy controls. sIL-2Rα serum levels are higher in patients with non-neoplastic diseases and benign tumors than in those with malignant neoplasia. There is a strong correlation between sIL-2Rα and leukocyte count. Conclusion: Measurements of sIL-2Rα in serum may be helpful in detecting stages and grades of inflammation in the progression of disease. sIL-2Rα could actually not be used as an indicator for malignant diseases in dogs like in humans. The strong correlation between sIL-2Rα and the leukocyte count indicates the inflammatory response to the disease. This could be helpful in giving a prognosis in some cases, because the inflammatory reaction is of prognostic relevance in different diseases including malignant and non-malignant neoplasia. Although the results of our research studies were very promising, further studies should be performed with a canine ELISA.
