期刊文献+
共找到329篇文章
< 1 2 17 >
每页显示 20 50 100
Implication of platelet-derived growth factor receptor alpha in prostate cancer skeletal metastasis 被引量:3
1
作者 Qingxin Liu Danielle Jernigan +1 位作者 Yun Zhang Alessandro Fatatis 《Chinese Journal of Cancer》 SCIE CAS CSCD 北大核心 2011年第9期612-619,共8页
Metastasis represents by far the most feared complication of prostate carcinoma and is the main cause of death for patients.The skeleton is frequently targeted by disseminated cancer cells and represents the sole site... Metastasis represents by far the most feared complication of prostate carcinoma and is the main cause of death for patients.The skeleton is frequently targeted by disseminated cancer cells and represents the sole site of spread in more than 80% of prostate cancer cases.Compatibility between select malignant phenotypes and the microenvironment of colonized tissues is broadly recognized as the culprit for the organ-tropism of cancer cells.Here,we review our recent studies showing that the expression of platelet-derived growth factor receptor alpha(PDGFR a) supports the survival and growth of prostate cancer cells in the skeleton and that the soluble fraction of bone marrow activates PDGFR a in a ligand-independent fashion.Finally,we offer pre-clinical evidence that this receptor is a viable target for therapy. 展开更多
关键词 前列腺癌细胞 生长因子受体 血小板 衍生 并发症 兼容性 微环境 殖民化
下载PDF
Distribution of platelet-derived growth factor-alpha receptor expressing oligodendrocyte precursor cells in the adult rat brain
2
作者 Hongjun Yu Jun Fei +1 位作者 Xue Luo Zhongxiang Yao 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第12期1093-1098,共6页
BACKGROUND: Studies have demonstrated that NG2-positive glial cells in the adult rats are predominantly located in the gray and white matter of the cerebral cortex and hippocampus. Platelet-derived growth factor-a re... BACKGROUND: Studies have demonstrated that NG2-positive glial cells in the adult rats are predominantly located in the gray and white matter of the cerebral cortex and hippocampus. Platelet-derived growth factor-a receptor (PDGF-αR) cells are a subset of oligodendrocytes, which are not as mature as NG2-positive cells. Distribution and migration of PDGF-αR-positive cells in the rat brain remain poorly understood. OBJECTIVE: Using immunohistochemical methods, the distribution of oligodendrocyte precursor cells (PDGF-αR-positive) was analyzed in the adult rat brain. DESIGN, TIME AND SETTING: Immunohistochemical study was performed at the Department of Histology and Embryology of the Third Military Medical University from September 2007 to September 2008. MATERIALS: Rabbit anti-PDGF-αR polyclonal antibody was purchased from Santa Cruz Biotechnology, USA. Streptomycin-avidin-biotin complex immunohistochemistry kit was purchased from Zhongshan Goldenbridge Biotechnology, China. METHODS: Whole brains from 5 healthy, adult, Wistar rats were collected for immunohistochemistry, and the mean value of PDGF-αR-expressing cells was quantified. The absolute values were translated to ranked data of high, moderate, and low grades (high grade: 10 positive cells; moderate grade: 5-9 cells; low grade: 〈 5 cells in a 400 × visual field). Based on the number of cell processes and branches, as well as the number of PDGF-αR-positive cells, in different regions, the cells were classified into three categories, i.e., type Ⅰ-Ⅲ. From type I to type Ill, the number of processes gradually increased. MAIN OUTCOME MEARSURES: The number and distribution of PDGF-αR-positive cells in different brain regions of adult rats. RESULTS: PDGF-αR-positive cells were located in the forebrain and midbrain, but not in the cerebellum or brainstem. In the olfactory bulb and hippocampus, a total of 60% PDGF-αR-positive cells were type Ⅰ and these cells were not mature as others. In the cerebral cortex, olfactory system, hippocampus, and optic chiasma, where neuronal bodies aggregated, approximately 40% of the PDGF-αR-positive cells were type Ⅱ, with few type Ⅲ cells. In the white matter, corpus callosum, basal nucleus, and thalamus, PDGF-αR-positive cell density was moderate. In the olfactory bulb and hippocampus, PDGF-αR-positive cell density was high. PDGF-αR-positive cells were not observed in the cerebellum or brainstem CONCLUSION: PDGF-αR-positive cells were aggregated in the olfactory bulb and hippocampus in the adult, rat brain, but few cells were detected in the cerebellum and brainstem. 展开更多
关键词 platelet-derived growth factor receptor oligodendrocyte progenitor cells brain DISTRIBUTION
下载PDF
Expression of NG2 and platelet-derived growth factor receptor alpha in the developing neonatal rat brain
3
作者 Ping Li Heng-xi Li +4 位作者 Hong-yan Jiang Lie Zhu Hai-ying Wu Jin-tao Li Jiang-hua Lai 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第11期1843-1852,共10页
Platelet-derived growth factor receptor alpha (PDGFRct) is a marker of oligodendrocyte precursor cells in the central nervous system. NG2 is also considered a marker of oligodendrocyte precursor cells. However, whet... Platelet-derived growth factor receptor alpha (PDGFRct) is a marker of oligodendrocyte precursor cells in the central nervous system. NG2 is also considered a marker of oligodendrocyte precursor cells. However, whether there are differences in the distribution and morphol- ogy of oligodendrocyte precursor cells labeled by NG2 or PDGFRa in the developing neonatal rat brain remains unclear. In this study, by immunohistochemical staining, NG2 positive (NG2+) cells were ubiquitous in the molecular layer, external pyramidal layer, internal pyramidal layer, and polymorphic layer of the cerebral cortex, and corpus callosum, external capsule, piriform cortex, and medial septal nucleus. NG2~ cells were stellate or fusiform in shape with long processes that were progressively decreased and shortened over the course of brain development. The distribution and morphology of PDGFRct positive (PDGFRa+) cells were coincident with NG2+ cells. The co- localization of NG2 and PDGFRu in the cell bodies and processes of some cells was confirmed by double immunofluorescence labeling. Moreover, cells double-labeled for NG2 and PDGFRa were predominantly in the early postnatal stage of development. The numbers of NG2+/PDGFRa+ cells and PDGFRa+ cells decreased, but the number of NG2+ cells increased from postnatal days 3 to 14 in the developing brain. In addition, amoeboid microglial cells of the corpus callosum, newborn brain macrophages in the normal developing brain, did not express NG2 or PDGFRu, but NG2 expression was detected in amoeboid microglia after hypoxia. The present results suggest that NG2 and PDGFRct are specific markers of oligodendrocyte precursor cells at different stages during early development. Additionally, the NG2 protein is involved in inflammatory and pathological processes of amoeboid microglial cells. 展开更多
关键词 nerve regeneration NG2 platelet-derived growth factor receptor alpha oligodendrocyte precursor cells amoeboid microglial cells OX-42 HYPOXIA cerebral cortex corpus callosum neural regeneration
下载PDF
Deficiency of platelet-derived growth factor receptor-α-positive cells in Hirschsprung's disease colon 被引量:3
4
作者 Anne-Marie O'Donnell David Coyle Prem Puri 《World Journal of Gastroenterology》 SCIE CAS 2016年第12期3335-3340,共6页
AIM: To investigate whether the expression of platelet-derived growth factor receptor-&#x003b1;-positive (PDGFR&#x003b1;<sup>+</sup>)-cells is altered in Hirschsprung&#x02019;s disease (HD).MET... AIM: To investigate whether the expression of platelet-derived growth factor receptor-&#x003b1;-positive (PDGFR&#x003b1;<sup>+</sup>)-cells is altered in Hirschsprung&#x02019;s disease (HD).METHODS: HD tissue specimens (n = 10) were collected at the time of pull-through surgery, while colonic control samples were obtained at the time of colostomy closure in patients with imperforate anus (n = 10). Immunolabelling of PDGFR&#x003b1;<sup>+</sup>-cells was visualized using confocal microscopy to assess the distribution of these cells, while Western blot analysis was undertaken to quantify PDGFR&#x003b1; protein expression.RESULTS: Confocal microscopy revealed PDGFR&#x003b1;<sup>+</sup>-cells within the mucosa, myenteric plexus and smooth muscle in normal controls, with a marked reduction in PDGFR&#x003b1;<sup>+</sup>-cells in the HD specimens. Western blotting revealed high levels of PDGFR&#x003b1; protein expression in normal controls, while there was a striking decrease in PDGFR&#x003b1; protein expression in the HD colon.CONCLUSION: These findings suggest that the altered distribution of PDGFR&#x003b1;<sup>+</sup>-cells in both the aganglionic and ganglionic HD bowel may contribute to the motility dysfunction in HD. 展开更多
关键词 platelet-derived growth factor receptor alpha Hirschsprung’ s disease Gastrointestinal motility AGANGLIONOSIS Myenteric plexus
下载PDF
Vascular endothelial growth factor/platelet-derived growth factor receptor pathway is involved in bone marrow mesenchymal stem cell differentiation and directional migration toward gliomas 被引量:1
5
作者 Chaoshi Niu Yongfei Dong Ge Gao 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第13期993-998,共6页
BACKGROUND: Vascular endothelial growth factor (VEGF) induces bone marrow-derived mesenchymal stem cell (BMSC) differentiation into vascular endothelial-like cells and promotes BMSC migration toward gliomas. Howe... BACKGROUND: Vascular endothelial growth factor (VEGF) induces bone marrow-derived mesenchymal stem cell (BMSC) differentiation into vascular endothelial-like cells and promotes BMSC migration toward gliomas. However, the molecular mechanisms by which VEGF induces BMSC differentiation and migration remain poorly understood. OBJECTIVE; To investigate the role of platelet-derived growth factor (PDGF) receptor (PDGFR) in BMSC differentiation and migration induced by VEGE DESIGN, TIME AND SETTING: A parallel, controlled, in vitro experiment was performed at the Molecular Neurobiology & Neural Regeneration and Repairing Laboratory, Anhui Provincial Hospital of Anhui Medical University, China from June 2008 to March 2009. MATERIALS: U87 glioma cells were purchased from Shanghai Institutes for Biological Sciences; mouse anti-human PDGFR and VEGF receptor (VEGFR) monoclonal antibodies were purchased from Peprotech, USA. METHODS: Isolated BMSCs were precultured with neutralizing antibody for VEGFR-1, VEGFR-2, PDGFR-α, and PDGFR-β to block biological activity of related receptors, followed by induced differentiation with 50μg/L VEGF. BMSCs induced with 50μg/L VEGF alone served as the VEGF-induced group. The control group remained untreated. MAIN OUTCOME MEASURES: Cell surface markers were identified by flow cytometry; BMSC surface cytokine receptor expression was detected by reverse transcription-polymerase chain reaction; the Transwell model was used to observe cell migration. RESULTS: After blocking the PDGFR, VEGF did not induce BMSC cell surface marker CD-31 or von Willebrand factor (vWF) expression. However, inhibition with VEGF receptor blocking agents, VEGF induced BMSCs to express CD-31 and vWE Following inhibition of the PDGFR, the number of cells migrating through the polycarbonate membrane Transwell chamber was decreased, as well as the number of BMSCs migrating to glioma cells. However, through the use of VEGF receptor blocking agents, the number of migrating cells remained unchanged. VEGF preculture increased the number of BMSCs migrating to gliomas. CONCLUSION: VEGF interacts with PDGFRs on the BMSC surface to attract BMSC directional migration and induce BMSC differentiation. The VEGF/PDGFR pathway participates in BMSC directional migration to glioma. VEGF pretreatment increased efficiency of BMSC migration to glioma. 展开更多
关键词 vascular endothelial growth factor platelet-derived growth factor receptor bone marrow-derived mesenchymal stem cells GLIOMA IMMUNOFLUORESCENCE
下载PDF
KIT and platelet-derived growth factor receptor α wild-type gastrointestinal stromal tumor associated with neurofibromatosis type 1: Two case reports 被引量:1
6
作者 You-Wei Kou Ying Zhang +1 位作者 Ya-Ping Fu Zhe Wang 《World Journal of Clinical Cases》 SCIE 2019年第24期4398-4406,共9页
BACKGROUND Gastrointestinal stromal tumors(GISTs) associated with neurofibromatosis are uncommon compared to their gastrointestinal counterparts. Patients with neurofibromatosis type 1(NF-1) have an increased risk of ... BACKGROUND Gastrointestinal stromal tumors(GISTs) associated with neurofibromatosis are uncommon compared to their gastrointestinal counterparts. Patients with neurofibromatosis type 1(NF-1) have an increased risk of developing gastrointestinal tumors, including rare types such as GIST.CASE SUMMARY A 60-year-old male Chinese patient was diagnosed with NF-1 10 years ago and presented with upper abdominal discomfort and black stools. Endoscopic ultrasonography and an enhanced abdominal computed tomography scan revealed a mass located 4 cm from the muscular layer of the descending duodenum. A 59-year-old Chinese woman who was diagnosed with NF-1 25 years ago presented with sudden unconsciousness and black stools. Multiple masses in the duodenum were noted by echogastroscopy and an enhanced abdominal computed tomography scan. Both patients presented with cutaneous neurofibromas. The histologic examination of tumors from both patients revealed spindle cells and low mitotic activity. Immunohistochemically, the tumor cells showed strong positivity for KIT(CD117), DOG-1, CD34, and Dehydrogenase Complex Subunit B, and negativity for SMA, desmin, S-100, and β-catenin. None of the six tumors from two patients had KIT exon 9, 11, 13, or 17 or platelet-derived growth factor receptor α exon 12 or 18 mutation, which is a typical finding for sporadic GISTs. None of the six tumors from the two patients had a BRAFV600 E mutation. The patients were alive and well during the follow-up period(range:0.6-5 yr).CONCLUSION There have been only a few previous reports of GISTs associated with NF-1.Although GISTs associated with NF-1 have morphologic and immunohistochemical similarities with GISTs, the pathogenesis, incidence,genetic background, and prognosis are not completely known. A medical history of NF-1 in a patient who has gastrointestinal bleeding or anemia and an intraabdominal mass with nonspecific computed tomography features may help in diagnosing GIST by virtue of the well-known association of these two entities.Molecular genetic studies of cases indicated that GISTs in NF-1 patients have a different pathogenesis than sporadic GISTs. 展开更多
关键词 NEUROFIBROMATOSIS Gastrointestinal stromal KIT and platelet-derived growth factor receptorαwild type Molecular genetic studies Neurofibromatosis type 1 Case report
下载PDF
Association Study of a Proliferation-inducing Ligand, Spermatogenesis Associated 8, Platelet-derived Growth Factor Receptor-alpha, and POLB Polymorphisms with Systemic Lupus Erythematosus in Chinese Han Population 被引量:6
7
作者 Ping Li Yuan Li +7 位作者 Ai-Hong Zhou Si Chen Jing Li Xiao-Ting Wen Zi-Yan Wu Liu-Bing Li Feng-Chun Zhang Yong-Zhe Li 《Chinese Medical Journal》 SCIE CAS CSCD 2016年第17期2085-2090,共6页
Background: Systemic lupus eiLythenaatosus (SLE) is a prototypic autoimmune disease wida complex genetic inheritance. This study was conducted to examine whether the association ofa proliferation-inducing ligand (... Background: Systemic lupus eiLythenaatosus (SLE) is a prototypic autoimmune disease wida complex genetic inheritance. This study was conducted to examine whether the association ofa proliferation-inducing ligand (APRIL), spermatogenesis associated 8 (SPATA8), platelet-derived growth lhctor receptor-alpha (PDGFRA), and DNA polymerase beta (POLB) with SLE can be replicated in a Chinese Han population. Methods: Chinese SLE patients (n = 1247) and ethnically and geographically matched healthy controls (n 1440) were genotyped for the APRI L, SPATAS. PDGFRA, and POLB single-nucleotide polymorphisms (SN Ps), rs3803800 rs8023715, rs1364089 and rsl2678588 using the Sequenom MassARRAY System. Results: The Chinese Hart SLE patients and controls had statistically similar liequencies of alleles and genotypes of four gene polynlorphisms. Moreover, no association signal was detected on different genetic models (additive, dominant, and recessive, all, P〉 0.05) or in SLE subgroups stratified by various clinical nlanifestations (all, P 〉 0.05). Conclusions: Different genetic backgrounds from different ancestries and various populations may result in different genetic risk litctors for SLE. We did not detect any significant association with SNPs of APRIL SPATAS, PDGFRA, and POLB. 展开更多
关键词 A Proliferalion-inducing Ligand Genetic Association Spermatogenesis Associated 8 Systemic Lupus Erythelnatosus platelet-derivcd growth factor receptor-alpha POLB
原文传递
Basic fibroblast growth factor gene transfection in repair of internal carotid artery aneurysm wall
8
作者 Lei Jiao Ming Jiang +3 位作者 Jinghai Fang Yinsheng Deng Zejun Chen Min Wu 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第36期2915-2921,共7页
Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external ca... Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external carotid arteries of New Zealand rabbits. Following model induction, lentivirus carrying basic fibroblast growth factor was injected through the ear vein. We found that the longer the action time of the lentivirus, the smaller the aneurysm volume. Moreover, platelet-derived growth factor expression in the aneurysm increased, but smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression decreased. At 1,2, 3, and 4 weeks following model establishment, following 1 week of injection of lentivirus carrying basic fibroblast growth factor, the later the intervention time, the more severe the blood vessel damage, and the bigger the aneurysm volume, the lower the smooth muscle 22 aJpha and hypertension-related gene ~ mRNA expression. Simultaneously, platelet-derived growth factor expression decreased. These data suggest that recombinant lentivirus carrying basic fibroblast growth factor can repair damaged cells in the aneurysmal wall and inhibit aneurysm dynamic growth, and that the effect is dependent on therapeutic duration. 展开更多
关键词 basic fibroblast growth factor LENTIVIRUS ANEURYSM vascular smooth muscle cells hypertension-related gene 1 smooth muscle 22 alpha platelet-derived growth factor gene therapy brain injury neural regeneration
下载PDF
Effects of ribozyme targeting platelet-derived growth factor receptor β subunit gene on the proliferation and apoptosis of hepatic stellate cells in vitro 被引量:18
9
作者 CHENYue-xiang LUCui-hua +5 位作者 XIEWei-fen ZHANGXing-rong ZHANGZhong-bing WEILi-xin JINYou-xin GUOYa-jun 《Chinese Medical Journal》 SCIE CAS CSCD 2005年第12期982-988,共7页
Background Activation and proliferation of hepatic stellate cells (HSC) is essentially involved in the development and progression of hepatic fibrosis. The most potent growth factor for HSC is platelet-derived growth... Background Activation and proliferation of hepatic stellate cells (HSC) is essentially involved in the development and progression of hepatic fibrosis. The most potent growth factor for HSC is platelet-derived growth factor receptor (PDGF) and PDGF receptor β subunit (PDGFR-β) is the predominant signal transduction pathyway of PDGF which is overexpressed in activated HSC. This study investigated the cleavage activity of hammerhead ribozyme targeting PDGFR-β mRNA in HSC and the effect on biological characteristics of HSC.Methods Expression vector of anti-PDGFR-β ribozyme was constructed and transfected into rat activated HSC with lipofectamin. The positive cell clones were gained by G418 selection. The expression of PDGFR-β, α-smooth muscle actin, and typeⅠand type Ⅲ collagen were detected by using Northern blot, Western blot and immunocytochemical staining, respectively. The cell proliferation was determined with MTT colorimetric assay. The cell apoptosis was analyzed by using flow cytometry, acridine orange fluorescence vital staining and transmission electron microscopy.Results The expression of PDGFR-β at mRNA and protein level was markedly reduced in ribozyme-transfected HSC by 49%-57% ( P <0.05-0.01). The proliferation and α-smooth muscle actin expression of ribozyme-transfected HSC were significantly decreased ( P <0.05-0.01), and the type Ⅰ and type Ⅲ collagen synthesis were also reduced ( P <0.01). In addition, the proliferative response of ribozyme-transfected HSC to PDGF BB was significantly inhibited. Otherwise, the apoptotic cells were significantly increased in ribozyme-transfected HSC ( P <0.01), and typical apoptotic cells could be found under transmission electron microscopy.Conclusions The anti-PDGFR-β ribozyme effectively cleaved the target RNA and significantly inhibited its expression, which blocked the signal transduction of PDGF at receptor level, inhibited HSC proliferation and collagen synthesis, and induced HSC apoptosis. These results suggest that inhibiting PDGFR-β expression of HSC may be a new target for the therapy of liver fibrogenesis, and ribozyme may be a useful tool for inhibiting PDGFR-β expression. 展开更多
关键词 RIBOZYME receptor platelet-derived growth factor hepatic stellate cell liver fibrosis
原文传递
PDGFR-β/TGF-β/Smad2/3信号通路调控阿尔茨海默病血脑屏障完整性和学习记忆能力的分子机制研究 被引量:1
10
作者 申杰 姚雪榕 +1 位作者 刘越存 徐桂华 《神经损伤与功能重建》 2024年第1期1-7,共7页
目的:分析PDGFR-β/TGF-β/Smad2/3信号通路调控阿尔茨海默病(AD)血脑屏障(BBB)完整性和学习记忆能力的分子机制。方法:利用APP/PS1转基因AD小鼠模型,通过水迷路及觅食试验分析学习记忆能力;荧光免疫组织化学法检测海马区血管周细胞增殖... 目的:分析PDGFR-β/TGF-β/Smad2/3信号通路调控阿尔茨海默病(AD)血脑屏障(BBB)完整性和学习记忆能力的分子机制。方法:利用APP/PS1转基因AD小鼠模型,通过水迷路及觅食试验分析学习记忆能力;荧光免疫组织化学法检测海马区血管周细胞增殖率(ki67/desmin)、周细胞覆盖率(desmin/lectin);Western blot检测海马区血管周细胞TGF-βR1及其下游信号通路分子、紧密连接(TJs)蛋白的表达水平。经过外源性PDGF-BB脑室内注射和/或TGF-βR1激酶抑制剂SB431542腹腔内注射预处理后,分别进行上述分析。构建AD体外BBB模型,经过PDGF-BB和/或SB431542作用后,进行异硫氰酸荧光素-牛血清白蛋白(FITC-BSA)渗透性和跨细胞电阻检测。结果:与对照组相比,APP/PS1小鼠经过虚拟平台次数明显减少,达到终点所需时间明显延长(水迷路训练试验),投食区正确选择率明显下降(觅食训练试验);海马区desmin/lectin阳性细胞比例明显下降;TGF-βR1、p-Smad2、p-Smad3蛋白表达水平明显升高;TJs蛋白表达水平明显下降。外源性PDGF-BB可使APP/PS1小鼠经过虚拟平台次数明显增加、达到终点所需时间明显缩短(水迷路正式试验第28天)、投食区正确选择率明显提高(觅食正式试验第28天);海马区desmin/lectin阳性细胞比例明显增加;使TGF-βR1、p-Smad2、p-Smad3蛋白表达水平明显升高;使TJs蛋白表达水平明显升高。SB431542则可部分抑制上述作用。体外试验证明:外源性PDGF-BB可明显降低AD模型组的最终渗透系数,提高24 h时相对TEER值;SB431542则可部分抑制上述作用。结论:PDGFR-β/TGF-β/Smad2/3信号通路可通过诱导周细胞分化、覆盖,提高内皮细胞TJs的表达,调控AD血脑屏障完整性,以促进学习记忆能力恢复。 展开更多
关键词 阿尔茨海默病 血小板源性生长因子受体β 转化生长因子-β 血脑屏障完整性 学习记忆能力
下载PDF
电针通过PLC/IP3通路改善功能性消化不良大鼠胃肠动力障碍
11
作者 杨德茜 陈琪 +1 位作者 金舒文 徐派的 《实用医学杂志》 CAS 北大核心 2024年第16期2284-2290,共7页
目的确定电针是否调节了血小板衍生生长因子受体α阳性(PDGFRα+)细胞中磷脂酶C(PLC)/肌醇-1,4,5-三磷酸酯(PLC/IP3)通路,从而改善功能性消化不良(FD)胃肠动力障碍。方法将40只SD大鼠随机分为5组:空白组、模型组、电针组、U73122(PLC抑... 目的确定电针是否调节了血小板衍生生长因子受体α阳性(PDGFRα+)细胞中磷脂酶C(PLC)/肌醇-1,4,5-三磷酸酯(PLC/IP3)通路,从而改善功能性消化不良(FD)胃肠动力障碍。方法将40只SD大鼠随机分为5组:空白组、模型组、电针组、U73122(PLC抑制剂)组、U73122+电针组,每组8只。除空白组外所有大鼠采用多因素应激干预法建立FD大鼠模型。造模成功后,U73122组予以腹腔注射抑制剂,电针组取足三里和太冲穴,U73122+电针组在针刺前2 h注射抑制剂。10 d后行胃肠动力学检测;采用免疫印迹法检测PDGFRα、PLC、P-PLC、IP3的蛋白表达水平;用免疫荧光检测PDGFRα和PLC、IP3的平均荧光密度和共定位表达情况;电子显微镜观察胃窦区域缝隙连接(GJ)情况。结果造模后大鼠胃肠动力减弱,PDGFRα、PLC和IP3的蛋白表达水平显著降低,GJ增宽,细胞形态改变;与模型组比较,电针组、U73122组和U73122+电针组大鼠胃肠动力显著改善,胃窦PDGFRα、PLC、P-PLC、IP3表达水平上升,GJ稍紧密,细胞形态恢复;U73122组和U73122+电针组胃窦PDGFRα、PLC、P-PLC、IP3表达水平无明显差异;PDGFRα与PLC和IP3存在荧光共定位。结论电针通过激活PDGFRα+细胞中的PLC/IP3通路改善FD大鼠的胃肠动力障碍。 展开更多
关键词 功能性消化不良 胃肠动力障碍 血小板衍生生长因子受体α阳性细胞 磷脂酶C 肌醇-1 4 5-三磷酸酯
下载PDF
PDGFRα^(+)细胞上P2Y1-SK3通路对功能性消化不良大鼠胃肠动力的调控机制
12
作者 杨德茜 陈琪 +1 位作者 潘小丽 徐派的 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第5期599-607,共9页
目的探究血小板衍生生长因子受体α^(+)(PDGFRα^(+))细胞上P2Y1-小电导Ca^(2+)激活K^(+)(SK3)通道对功能性消化不良(FD)大鼠胃肠动力的影响。方法将30只SD大鼠随机分为空白组、模型组、P2Y1受体抑制剂(MRS2500)组,每组10只。除空白组外... 目的探究血小板衍生生长因子受体α^(+)(PDGFRα^(+))细胞上P2Y1-小电导Ca^(2+)激活K^(+)(SK3)通道对功能性消化不良(FD)大鼠胃肠动力的影响。方法将30只SD大鼠随机分为空白组、模型组、P2Y1受体抑制剂(MRS2500)组,每组10只。除空白组外,其余两组采用多因素干预法建立FD大鼠模型。造模成功后抑制剂组予以尾静脉注射P2Y1抑制剂MRS2500,其他组不采取干预措施。处理结束后进行行为学和胃肠动力学检测;用BL-420S生物信号系统采集并分析胃肠生物电信息;取胃窦组织评估病理变化;采用免疫印迹、实时荧光定量PCR技术检测各组大鼠胃窦PDGFRα、C-kit(卡介尔间质细胞特异性指标)、P2Y1和SK3的表达情况;采用免疫荧光法检测胃窦PDGFRα和C-kit、P2Y1、SK3的组织表达和共定位情况;用钙检测试剂盒检测胃窦组织中Ca^(2+)含量变化。结果FD模型建立后,大鼠活动度、体重增长速度和进食量都显著降低,胃肠动力减弱,胃窦内PDGFRα、C-kit、P2Y1和SK3表达水平降低。MRS2500干预后,P2Y1受体抑制剂组大鼠较模型组大鼠体重增长率和进食量升高,胃肠动力减弱情况改善,PDGFRα、P2Y1和SK3表达水平进一步降低,C-kit表达水平升高,Ca^(2+)含量降低。PDGFRα与C-kit在胃窦中不存在共表达,而PDGFRα与P2Y1、SK3共表达。结论长期的饮食和情绪失调会刺激肠神经系统释放抑制性神经递质,这一过程通过PDGFRα^(+)细胞上P2Y1受体引起SK3通道的Ca^(2+)敏感性降低,在多因素刺激诱导的FD模型大鼠胃肠动力障碍中起重要作用。 展开更多
关键词 功能性消化不良 血小板衍生生长因子受体α^(+)细胞 卡介尔间质细胞 P2Y1 小电导Ca^(2+)激活K^(+)通道
下载PDF
自发性高血压大鼠中血小板源生长因子-AA及其受体表达与血管平滑肌细胞增殖的关系 被引量:7
13
作者 晋军 祝善俊 +2 位作者 祝之明 杨永健 丁钢 《生理学报》 CAS CSCD 北大核心 2002年第2期145-148,共4页
为明确血小板源生长因子 AA(plateletderivedgrowthfactor AA ,PDGF AA)及PDGF α受体在自发性高血压大鼠 (spontaneouslyhypertensionrats,SHR)血管平滑肌细胞 (vascularsmoothmusclecells,VSMCs)增殖中的作用 ,采用Westernblot、[3 H]... 为明确血小板源生长因子 AA(plateletderivedgrowthfactor AA ,PDGF AA)及PDGF α受体在自发性高血压大鼠 (spontaneouslyhypertensionrats,SHR)血管平滑肌细胞 (vascularsmoothmusclecells,VSMCs)增殖中的作用 ,采用Westernblot、[3 H]TdR及 [3 H]Leu掺入率等方法 ,观察在SHR和WKY大鼠VSMC中PDGF AA及PDGF受体表达的差异 ;在PDGF AA刺激下VSMC增殖和肥大反应的变化。结果显示 ,SHR VSMC中PDGF AA、PDGF α受体蛋白表达明显高于WKY VSMC(P <0 0 1) ,而PDGF β受体蛋白表达在SHR VSMC与WKY VSMC无明显差异 ;在不同浓度PDGF AA刺激下 ,增殖细胞核抗原 (PCNA)及3 H掺入率在SHR VSMC明显增强且呈剂量依赖性增加 (P <0 0 1)。本研究表明PDGF A链及其α受体的自泌性增高 ,可能是导致SHR VSMC异常增殖和肥大 。 展开更多
关键词 自发性高血压 大鼠 血小板源生长因子-AA 受体表达 血管平滑机细胞增殖
下载PDF
血小板源性生长因子和受体在皮肤溃疡中表达特征及其对溃疡形成影响的研究 被引量:10
14
作者 陈伟 付小兵 +3 位作者 孙同柱 杨银辉 赵志力 盛志勇 《中国危重病急救医学》 CAS CSCD 2002年第9期515-517,F003,共4页
目的 :观察血小板源性生长因子 (PDGF)的两个亚基 (A、B)和两种受体 (PDGFRα和 PDGFRβ)在正常皮肤、溃疡边缘和溃疡组织中的分布、表达特征及其与溃疡形成的关系。方法 :2 4份被测标本来自 8例不同类型的溃疡患者 ,取其溃疡皮肤组织 ... 目的 :观察血小板源性生长因子 (PDGF)的两个亚基 (A、B)和两种受体 (PDGFRα和 PDGFRβ)在正常皮肤、溃疡边缘和溃疡组织中的分布、表达特征及其与溃疡形成的关系。方法 :2 4份被测标本来自 8例不同类型的溃疡患者 ,取其溃疡皮肤组织 8份、对应的溃疡边缘组织 8份及正常皮肤组织 8例。用常规病理技术和免疫组织化学方法确定这 4种蛋白在不同组织中的定位和表达量的变化规律。结果 :在正常皮肤组织中 ,PDGF A和 PDGF B的阳性颗粒主要见于表皮基底层细胞和内皮细胞中 ,两个亚基的阳性细胞率分别为(15 .2± 5 .6 ) %和 (2 2 .4± 7.4 ) % ;PDGFRα存在于表皮细胞和内皮细胞的细胞膜上 ,该受体表达较多 ,阳性表达率为 (36 .7± 4 .3) % ;PDGFRβ在正常皮肤中表达较弱 (15 .3± 4 .8) % ,蛋白颗粒主要集中于表皮基底层细胞膜上。从正常皮肤组织经溃疡边缘到溃疡中心 ,PDGF A和 PDGF B的蛋白表达呈升高趋势。在溃疡组织中 ,PDGF A和 PDGF B蛋白主要分布于角质形成细胞、单核细胞和巨噬细胞的胞质内 ,两者的阳性细胞率分别升至为正常皮肤的 1.4 1倍和 1.13倍。 PDGFRα主要分布于表皮基底层细胞和内皮细胞的细胞膜上 ,而含有 PDGFRβ阳性颗粒的细胞主要为表皮生发层细胞。与正常皮肤组织相比 ,α和β型受体表? 展开更多
关键词 溃疡形成 血小板源性生长因子 血小板源性生长因子受体 难愈性皮肤溃疡 皮肤组织 发病机制
下载PDF
PDGF和PDGFR与肿瘤的关系及其靶向治疗研究进展 被引量:21
15
作者 陈旭升 孙丹 姚欣 《中国肿瘤临床》 CAS CSCD 北大核心 2012年第15期1134-1137,共4页
血小板衍生生长因子(platelet-derived growth factor,PDGF)是从人的血小板中分离出来的促血管生成因子。血小板衍生生长因子受体(platelet-derived growth factor receptor,PDGFR)是酪氨酸蛋白激酶家族成员,能够促进细胞的趋化、分裂... 血小板衍生生长因子(platelet-derived growth factor,PDGF)是从人的血小板中分离出来的促血管生成因子。血小板衍生生长因子受体(platelet-derived growth factor receptor,PDGFR)是酪氨酸蛋白激酶家族成员,能够促进细胞的趋化、分裂与增殖;在机体生长发育、创伤修复等生理过程中起着积极重要的作用。PDGF/PDGFR作为血管生成因子之一与肿瘤的发生发展有密切关系,肿瘤细胞释放的PDGF能诱导血管内皮细胞、平滑肌细胞和肿瘤细胞的增殖和迁移,并抑制其凋亡。以PDGF/PDGFR为靶点的靶向治疗也已取得了较大进展。本文就PDGF/PDGFR在肿瘤发生、发展中的作用及靶向治疗方面的进展加以综述。 展开更多
关键词 血小板衍生生长因子 血小板衍生生长因子受体 肿瘤 靶向治疗
下载PDF
选择性PDGF受体酪氨酸激酶抑制剂对兔PVR的治疗作用 被引量:4
16
作者 任百超 权彦龙 +3 位作者 郑玉萍 孙乃学 生野恭司 田野保雄 《西安交通大学学报(医学版)》 CAS CSCD 北大核心 2003年第3期267-270,共4页
目的 评价一种新的血小板源性生长因子 (platelet derivedgrowthfactor,PDGF)受体酪氨酸激酶抑制剂AG12 95对兔增殖性玻璃体视网膜病变 (proliferativevitreoretinopathy ,PVR)的治疗作用。 方法 兔结膜成纤维细胞 (rabbitconjunctiva... 目的 评价一种新的血小板源性生长因子 (platelet derivedgrowthfactor,PDGF)受体酪氨酸激酶抑制剂AG12 95对兔增殖性玻璃体视网膜病变 (proliferativevitreoretinopathy ,PVR)的治疗作用。 方法 兔结膜成纤维细胞 (rabbitconjunctivalfibroblastscells,RCF)培养 ,用MTT分析法检测PDGF AA和 BB以及AG12 95对兔RCF的增殖状况的影响。建立PVR动物模型 ,玻璃体腔内给予AG12 95 ,用牵引性视网膜脱离 (tractionalretinaldetach ment,TRD)的发生率判断药物的体内疗效。眼视网膜电生理检查和HE染色分析药物的毒性。结果  10 μmol·L-1和 10 0 μmol·L-1两种浓度的AG12 95均可显著抑制由PDGF AA和 BB诱导的成纤维细胞的增生 ,10 0 μmol·L-1AG12 95可减缓兔TRD的发生 ,但其作用仅持续至第 2 1d。在AG12 95治疗组中 ,未发现明显的网膜毒性。结论 PDGF受体酪氨酸激酶抑制剂AG12 95可减缓兔TRD的发生。 展开更多
关键词 选择性PDGF受体 酪氨酸激酶抑制剂 PVR 治疗 血小板源性生长因子 增殖性玻璃体视网膜病变
下载PDF
血小板源生长因子受体介导的信号转导在自发性高血压大鼠心肌肥大中的作用(英文) 被引量:5
17
作者 刘洁 白桦 +2 位作者 邢东琦 孙银平 吴立玲 《生理学报》 CAS CSCD 北大核心 2002年第2期159-164,共6页
为了探索血小板源生长因子 (platelet derivedgrowthfactor,PDGF)受体介导的信号转导在自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)心肌肥大中的作用 ,实验采用Westernblot法检测SHR及其对照WKY大鼠心肌PDGF受体β和细胞外信... 为了探索血小板源生长因子 (platelet derivedgrowthfactor,PDGF)受体介导的信号转导在自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)心肌肥大中的作用 ,实验采用Westernblot法检测SHR及其对照WKY大鼠心肌PDGF受体β和细胞外信号调节激酶 (extracellularsignal regulatedkinase1/ 2 ,ERK 1/ 2 )的蛋白表达和ERK 1/ 2磷酸化水平的变化。结果显示 :4周龄SHR的收缩压、舒张压、±dp/dtmax和心肌肥大指数与同龄WKY大鼠相比均无明显差异 ,而 12周龄SHR上述指标与同龄WKY大鼠相比均明显升高 ,表明 12周SHR已发生高血压 ,心脏收缩功能代偿性增强 ,并出现心肌肥大。 4周龄SHR心肌PDGF受体 β和ERK1/ 2的磷酸化水平以及ERK 1/ 2的蛋白表达水平与同龄对照相比均无明显变化 ,12周时SHR心肌PDGF受体 β的蛋白表达较同龄WKY增加 32 77% (P <          0 0 5 ) ,PDGF受体介导的信号转导通路的下游信号分子ERK 1/ 2的磷酸化水平较同龄WKY升高 19 6 % (P =0 0 1) ,表明ERK 1/ 2的活化增加 ,但ERK 1/ 2的蛋白表达水平尚无变化。为进一步明确PDGF受体 β在心肌细胞生长中的作用及其与ERK 1/ 2活性的关系 ,采用PDGF BB刺激培养的乳鼠心肌细胞 ,发现 [3 H]亮氨酸掺入量明显增加 ,ERK 1/ 展开更多
关键词 血小板源生长因子受体 介导 信号转导 自发性高血压 大鼠 心肌肥大
下载PDF
血小板源性生长因子受体α在兔增殖性玻璃体视网膜病变中的作用(英文) 被引量:6
18
作者 熊蕾 权颜龙 +3 位作者 郑玉萍 张利敏 张璐琰 熊全臣 《国际眼科杂志》 CAS 2008年第1期6-9,共4页
目的:血小板源性生长因子(platelet-derived growth factor,PDGF)可引起增殖性玻璃体视网膜病变。酪氨酸激酶的激活对生长因子介导的细胞增殖起着重要作用。本研究评价特异性的PDGF-α受体酪氨酸激酶阻断剂AG1296和β受体酪氨酸激酶阻断... 目的:血小板源性生长因子(platelet-derived growth factor,PDGF)可引起增殖性玻璃体视网膜病变。酪氨酸激酶的激活对生长因子介导的细胞增殖起着重要作用。本研究评价特异性的PDGF-α受体酪氨酸激酶阻断剂AG1296和β受体酪氨酸激酶阻断剂AG1295对兔PVR的治疗作用。方法:兔结膜成纤维细胞(rabbit conjunctival fibroblasts,RCF)培养,用MTT法检测PDGF-AA和-BB以及AG1295和AG1296对兔RCF增殖状况的影响。眼视网膜电图检查和HE染色分析药物的毒性。建立PVR动物模型,玻璃体腔内分别给予AG1295和AG1296,用牵引性视网膜脱离(tractional retinal detachment, TRD)的发生率评价药物的体内疗效。结果:体外10μmol/L的AG1295和AG1296均可显著抑制由PDGF-AA和-BB诱导的成纤维细胞的增生,体内100μmol/LAG1295和AG1296均减缓了兔TRD的发生,但AG1295的作用仅持续至14d。相同浓度的AG1296和AG1295相比,作用更持久。在两个治疗组中,均未发现明显的视网膜毒性。结论:特异性的PDGF-α受体酪氨酸激酶抑制剂AG129可显著抑制兔TRD的发生,其作用明显强于PDGF-β受体酪氨酸激酶抑制剂AG1295,提示PDGF对PVR的促进作用主要由α受体介导,这一通路的阻断可能成为治疗PVR的一种方法。 展开更多
关键词 血小板源性生长因子 受体酪氨酸激酶 AG1295 AG1296 增殖性玻璃体视网膜病变
下载PDF
VEGF、EGFR、PDGF在人脑胶质瘤中的表达及其与恶性程度和预后的关系 被引量:12
19
作者 李连进 佟建洲 +2 位作者 崔敬 吴红纪 王惠宾 《海南医学》 CAS 2016年第14期2251-2254,共4页
目的探讨血管内皮生长因子(VEGF)、表皮生长因子受体(EGFR)、血小板衍生生长因子(PDGF)在人脑胶质瘤中的表达及其与肿瘤恶性程度和患者预后的关系。方法选取我院神经外科2010年11月至2013年8月手术切除并经病理证实的脑胶质瘤标本68例... 目的探讨血管内皮生长因子(VEGF)、表皮生长因子受体(EGFR)、血小板衍生生长因子(PDGF)在人脑胶质瘤中的表达及其与肿瘤恶性程度和患者预后的关系。方法选取我院神经外科2010年11月至2013年8月手术切除并经病理证实的脑胶质瘤标本68例及同期正常脑组织标本12例,采用SP免疫组化法检测所有标本中VEGF、EGFR、PDGF的表达,分析其与胶质瘤各临床特征的关系,采用Spearman等级相关分析方法分析VEGF、EGFR、PDGF三者间的关系。结果胶质瘤组织中VEGF、EGFR和PDGF的表达阳性率分别为63.24%(43/68)、51.47%(35/68)和52.94%(36/68),均明显高于正常脑组织的0(0/12)、8.33%(1/12)和33.33%(4/12),差异均有统计学意义(P<0.05);在生存率方面,VEGF阳性患者低于VEGF阴性患者(23.26%vs 60.00%),EGFR阳性患者低于EGFR阴性患者(28.57%vs 51.52%),PDGF阳性患者低于PDGF阴性患者(27.78%vs 62.50%),差异均有统计学意义(P<0.05);Spearman等级相关分析显示,VEGF、EGFR及PDGF的表达与胶质瘤WHO分级呈显著正相关(r VEGF=0.428,r EGFR=0.407,r PDGF=0.431,P<0.05)。结论 VEGF、EGFR、PDGF在人脑胶质瘤发生发展中起重要调节作用,可作为判断胶质瘤恶性程度和预后的重要生物学参考指标。 展开更多
关键词 胶质瘤 血管内皮生长因子 表皮生长因子受体 血小板衍生生长因子 预后 相关性
下载PDF
血小板衍化生长因子β受体在人冠状动脉组织中的表达 被引量:8
20
作者 任雨笙 陈强 +2 位作者 贾国良 金岩 董绍忠 《第四军医大学学报》 1998年第6期652-654,共3页
目的:观察血小板衍化生长因子(PDGF)β受体在人冠状动脉(COA)组织中的分布与表达,研究PDGF及其受体在动脉粥样硬化形成过程中的作用机制.方法:年龄分别为21岁、47岁和64岁的男性尸检标本,取其COA前降支或左主干,以抗入PDGFβ... 目的:观察血小板衍化生长因子(PDGF)β受体在人冠状动脉(COA)组织中的分布与表达,研究PDGF及其受体在动脉粥样硬化形成过程中的作用机制.方法:年龄分别为21岁、47岁和64岁的男性尸检标本,取其COA前降支或左主干,以抗入PDGFβ受体多克隆抗体,应用免疫组化方法,观察其组织结构和PDGFβ受体的表达.结果:21岁的COA内皮细胞和平滑肌细胞无明显的PDGFβ受体阳性染色,47岁的COA可见内皮细胞和内膜下增生的平滑肌细胞有大量的阳性染色,64岁的COA内膜及内膜下病变区有集中的强阳性染色.不同年龄的COA血管其外膜结缔组织的成纤维细胞均呈阳性染色.结论:在COA受到损伤、修复或过度修复等不同状态下血管内皮细胞、平滑肌细胞膜上的PDGFβ受体的分布及表达亦有相应的改变.提示PDGF及PDGF受体在COA的粥样硬化形成过程中起着十分重要的作用. 展开更多
关键词 动脉粥样硬化 冠状动脉 PDGF 受体
下载PDF
上一页 1 2 17 下一页 到第
使用帮助 返回顶部