Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver ...Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.展开更多
目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-m...目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-mock转染的细胞作为对照组。细胞增殖通过Edu细胞荧光染色法检测,细胞凋亡通过Hoechst 33342细胞染色法检测。PTEN、AKT及pAKT的表达采用westernblot法检测。结果:实验组中SOX11 mRNA的相对表达量显著高于对照组[(354±26.2)%vs(100±1.3)%,P<0.05]。实验组细胞在450nm吸光(OD)值低于对照组(2.8±0.3 vs4.5±0.3,P<0.05)。Edu免疫荧光染色结果证实,实验组细胞增殖比例较对照组低[(12.4±4.3)%vs(32.3±3.3)%,P<0.05]。Hoechst 33342细胞凋亡荧光染色结果表明,实验组细胞凋亡比例较对照组高[(10.3±2.3)%vs(5.3±1.4)%,P<0.05]。Western blot实验结果表明,实验组PTEN蛋白的相对表达量高于对照组(0.6±0.03 vs 0.3±0.03,P<0.05),磷酸化Akt的相对表达量低于对照组(0.4±0.02 vs 0.7±0.02,P<0.05)。结论:过表达SOX11基因显著抑制人视网膜母细胞Y79的增殖并促使细胞凋亡。PTEN/Akt信号通路的激活可能参与该过程。展开更多
基金supported by grants from Guizhou Nursing Vocational College Foundation(No.gzhlyj2023-04)Guizhou Nursing Vocational College Foundation(No.gzhlyj2021-02)+1 种基金Science and Technology Foundation of Guizhou Provincial Health Committee(No.gzwkj2022-518)Nature Science Foundation of Beijing,China(No.7214253).
文摘Objective:SOX11 is expressed in numerous malignancies,including hepatocellular carcinomas(HCC),but its oncogenic function has not been elucidated.Here,we performed a comprehensive bioinformatics analysis of the Liver Hepatocellular Carcinoma(LIHC)dataset to investigate the function of SOX11 in tumorgenesis.Methods:SOX11 expression data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)were validated by immunohistochemistry(IHC).Co-expression,differential expression,and functional analyses utilized TCGA-LIHC,Timer 2.0,Metascape,GTEx,and LinkedOmics databases.Associations with immune infiltration,ferroptosis,and immune checkpoint genes were assessed.Genetic changes were explored via CBioPortal.Logistic regression,receiver operating characteristic curve(ROC),Kaplan-Meier analysis,and nomogram modeling evaluated associations with HCC clinicopathological features.SOX11’s impact on proliferation and migration was studied in HepG2 and HuH7 cell lines.Results:SOX11 was significantly elevated in HCC tumors compared to controls.SOX11-associated genes exhibited differential expression in pathways involving extracellular membrane ion channels.Significant associations were found between SOX11 levels,immune infiltration,ferroptosis,and immune checkpoint genes in HCC tissue.SOX11 levels correlated with HCC stage,histologic grade,and tumor status,and independently predicted overall and disease-specific survival.SOX11 expression effectively distinguished between tumor and normal liver tissue.Spearman correlations highlighted a significant relationship between SOX11 and ferroptosis-associated genes.Decreased SOX11 levels in HepG2 and HuH7 cells resulted in reduced proliferation and migration.Conclusions:SOX11 was found to represent a promising biomarker within HCC diagnosis and prognosis together with being a possible drug-target.
文摘目的:探讨过表达SOX11(Sex determination region of Y chromosome 11)基因对人视网膜母细胞瘤Y79细胞增殖、凋亡的影响及参与该过程的细胞信号传到途径。方法:将用重组pc DNA3.1-SOX11质粒转染的Y79细胞作为实验组,以空载体pc-DNA3.1-mock转染的细胞作为对照组。细胞增殖通过Edu细胞荧光染色法检测,细胞凋亡通过Hoechst 33342细胞染色法检测。PTEN、AKT及pAKT的表达采用westernblot法检测。结果:实验组中SOX11 mRNA的相对表达量显著高于对照组[(354±26.2)%vs(100±1.3)%,P<0.05]。实验组细胞在450nm吸光(OD)值低于对照组(2.8±0.3 vs4.5±0.3,P<0.05)。Edu免疫荧光染色结果证实,实验组细胞增殖比例较对照组低[(12.4±4.3)%vs(32.3±3.3)%,P<0.05]。Hoechst 33342细胞凋亡荧光染色结果表明,实验组细胞凋亡比例较对照组高[(10.3±2.3)%vs(5.3±1.4)%,P<0.05]。Western blot实验结果表明,实验组PTEN蛋白的相对表达量高于对照组(0.6±0.03 vs 0.3±0.03,P<0.05),磷酸化Akt的相对表达量低于对照组(0.4±0.02 vs 0.7±0.02,P<0.05)。结论:过表达SOX11基因显著抑制人视网膜母细胞Y79的增殖并促使细胞凋亡。PTEN/Akt信号通路的激活可能参与该过程。
